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Light Sheet Microscopy Incubation Design And Control Scheme, Venkatanathan Kidambi
Light Sheet Microscopy Incubation Design And Control Scheme, Venkatanathan Kidambi
Honors Scholar Theses
The focus of the following paper is the development of a light sheet or selective plane illumination microscopy (LSM / SPIM) platform to support long duration three dimensional imaging of various cells and tissues. This paper describes the complete design and fabrication process behind the prototype, including a definition of light sheet microscopy and its differences from confocal microscopy, as well as existing open source selective plane microscopy literature and designs. The complete engineering design process as applied to the microscope design is described in detail. Specifically, this paper will focus on the development of an incubation chamber for light …