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Photo-Thermal Coherent Confocal Microscope, Sean Sullivan, Charles Dimarzio
Photo-Thermal Coherent Confocal Microscope, Sean Sullivan, Charles Dimarzio
Charles A. DiMarzio
Confocal microscopy has been shown to be useful in imaging skin slightly below the junction of the dermis and epidermis. However, the depth of imaging is a significant limitation. We present a novel concept designed both to improve the depth of penetration and to increase the information content of images obtained with a reflectance confocal microscope. Using an approach similar to optoacoustics, we plan to explore the use of laser heating to generate tissue expansion, which will be measured by the microscope. The microscope will incorporate a pulsed heating laser along the same optical path as the imaging laser in …
Multi-Spectral Reflectance Confocal Microscopy On Skin, Tianchen Shi, Charles Dimarzio
Multi-Spectral Reflectance Confocal Microscopy On Skin, Tianchen Shi, Charles Dimarzio
Charles A. DiMarzio
Reflectance confocal microscopy is a powerful in-vivo modality for imaging superficial layers of biological tissue, especially for human skin. Three dimensional imaging capability enables confocal microscopy to resolve structures of upper skin layer cells. However, sub-celluar structure and corresponding functional organelles play more important parts in skin diseases diagnosis and monitoring. We present a new multi-spectral reflectance confocal microscopy to achieve sub-celluar functional imaging in skin by utilizing our unique Keck multi-modality microscope. Spectral information and a modified Mie scattering model are incorporated to identify distribution of melanin and mitochondria in cells. Ex-vivo and phantom experimental results are presented. Further …