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Full-Text Articles in Engineering
Effects Of Protein-Coated Nanofibers On Conformation Of Gingival Fibroblast Spheroids: Potential Utility For Connective Tissue Regeneration, Gili Kaufman, Ryan A. Whitescarver, Laiz Nunes, Xavier-Lewis Palmer, Drago Skrtic, Wojtek Tutak
Effects Of Protein-Coated Nanofibers On Conformation Of Gingival Fibroblast Spheroids: Potential Utility For Connective Tissue Regeneration, Gili Kaufman, Ryan A. Whitescarver, Laiz Nunes, Xavier-Lewis Palmer, Drago Skrtic, Wojtek Tutak
Engineering Technology Faculty Publications
Deep wounds in the gingiva caused bytrauma or surgery require a rapid and robust healing of connective tissues. Wepropose utilizing gas-brushed nanofibers coated with collagen and fibrin for that purpose. Our hypotheses are that protein-coated nanofibers will: (i) attract and mobilize cells in various spatial orientations, and (ii) regulate the expression levels ofspecific extracellular matrix (ECM)-associated proteins, determining the initial conformational nature ofdense and soft connective tissues. Gingival fibroblast monolayers and3D spheroids were cultured onECMsubstrate and covered with gas-blown poly-(DL-lactide-co-glycolide)(PLGA) nanofibers (uncoated/coated with collagen and fibrin). Cell attraction and rearrangement was followed byF-actin staining and confocal microscopy. Thicknesses ofthe cell …
Patient-Derived Hydrogel As A Sacrificial Matrix For Efficient Cell Loading, Shalmli U. Joshi
Patient-Derived Hydrogel As A Sacrificial Matrix For Efficient Cell Loading, Shalmli U. Joshi
Honors Scholar Theses
The field of tissue engineering focuses on delivering patient-derived stem cells to the body through the use of degradable biomaterials, such as hydrogels, which are infused into engineered scaffolds. Hydrogels act as templates to support and guide cells towards the regeneration of new tissue. In this study, we introduce a completely intraoperative procedure for obtaining a fibrin hydrogel for tissue engineering applications. In the past, fibrin hydrogel has been commonly formed by combining fibrinogen protein with animal-derived thrombin. Instead, we have developed an automated, and therefore reproducible, protocol to isolate and form fibrin hydrogel without the use of animal-derived thrombin. …
Investigation Of A New Material For Heart Valve Tissue Engineering, Claire Brougham, Nian Shen, Allison Cudsworth, Thomas Flanagan, Stefan Jockenhoevel, Fergal O'Brien
Investigation Of A New Material For Heart Valve Tissue Engineering, Claire Brougham, Nian Shen, Allison Cudsworth, Thomas Flanagan, Stefan Jockenhoevel, Fergal O'Brien
Conference Papers
No abstract provided.