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Synchronization And Media Exchange In Large-Scale Caenorhabditis Elegans Cultures, Jason D. Brown
Synchronization And Media Exchange In Large-Scale Caenorhabditis Elegans Cultures, Jason D. Brown
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The nematode Caenorhabditis elegans is a model organism for understanding sensory molecules of multicellular organisms. Ovulating hermaphrodites produce putative pheromone(s) that cause male attraction. Because pheromones are produced in such small quantities, adult conditioned-media from large-scale synchronous culture is necessary to analyze these pheromones. Current protocols for culture synchronization have volume constraints that limit large-scale synchronous cultures and current methodology for adult conditioned-media production is impractical.
Modification of Tangential Flow Filtration (TFF) systems was investigated for use as a method to increase the volume limits of bleach egg harvest for C. elegans culture synchronization. Also, an adult retention device built …