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Full-Text Articles in Engineering
Enzyme Optimization For Lignocellulose Hydrolysis Using Mechanistic Modeling, Adam S. Marlowe
Enzyme Optimization For Lignocellulose Hydrolysis Using Mechanistic Modeling, Adam S. Marlowe
Dissertations, Master's Theses and Master's Reports - Open
A novel mechanistic model for the saccharification of cellulose and hemicellulose is utilized to predict the products of hydrolysis over a range of enzyme loadings and times. The mechanistic model considers the morphology of the substrate and the kinetics of enzymes to optimize enzyme concentrations for the enzymatic hydrolysis of cellulose and hemicellulose simultaneously. Substrates are modeled based on their fraction of accessible sites, glucan content, xylan content, and degree of polymerizations. This enzyme optimization model takes into account the kinetics of six core enzymes for lignocellulose hydrolysis: endoglucanase I (EG1), cellobiohydrolase I (CBH1), cellobiohydrolase II (CBH2), and endo-xylanase (EX) …
Virus Purification, Detection And Removal, Khrupa Saagar Vijayaragavan
Virus Purification, Detection And Removal, Khrupa Saagar Vijayaragavan
Dissertations, Master's Theses and Master's Reports - Open
The biopharmaceutical industry has a growing demand and an increasing need to improve the current virus purification technologies, especially as more and more vaccines are produced from cell-culture derived virus particles. Downstream purification strategies can be expensive and account for 70% of the overall manufacturing costs. The economic pressure and purification processes can be particularly challenging when the virus to be purified is small, as in our model virus, porcine parvovirus (PPV). Our efforts are focused on designing an easy, economical, scalable and efficient system for virus purification, and we focused on aqueous two-phase systems. Industry acceptable standards for virus …