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Full-Text Articles in Engineering

Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek Jan 2020

Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek

CHEMISTRY AND CHEMICAL ENGINEERING

The article discusses use of the insect cell cultures for biotechnological purposes. Optimal conditions for the cultiva-tion of Spodoptera exigua and Lymantria dispar cell lines were selected.


Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek Jan 2020

Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek

CHEMISTRY AND CHEMICAL ENGINEERING

The article discusses use of the insect cell cultures for biotechnological purposes. Optimal conditions for the cultiva-tion of Spodoptera exigua and Lymantria dispar cell lines were selected.


Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek Oct 2019

Selection Of Optimal Conditions For The Cultivation Of Insect Cells For Use In Biotechnology, Khasanоv Shuhrat, Sasmakоv Sobir, Abdurakhmanоv Jaloliddin, Ashirоv Оybek

CHEMISTRY AND CHEMICAL ENGINEERING

The article discusses use of the insect cell cultures for biotechnological purposes. Optimal conditions for the cultiva-tion of Spodoptera exigua and Lymantria dispar cell lines were selected.


Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Rnacrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma Jul 1999

Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Rnacrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma

Papers in Biotechnology

To test for tumour necrosis-like factor (TNF) of chickens, supernatants of a lipopolysaccharide (LPS)-stimulated chicken macrophage cell line MQ-NCSU were analysed. A sequence of ion-exchange and gel-permeation chromatography was utilised to isolate TNF-like activity from the culture supernatant. The peak of TNF-like cytotoxic activity corresponded to the fractions with a nlolecular weight of 81 kDa or higher. Polyclonal anti-human TNF-a antiserum cross-reacted by Western blotting with a 17 kDa protein in the TNF-containing fraction ~ ~ n d e denaturing conditions. This result indicated that chicken TNF-like factor in the biologically active form may be a protein multimer of monomers …


Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Macrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma Jul 1999

Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Macrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma

Papers in Biotechnology

To test for tumour necrosis-like factor (TNF) of chickens, supernatants of a lipopolysaccharide (LPS)-stimulated chicken macrophage cell line MQ-NCSU were analysed. A sequence of ion-exchange and gel-permeation chromatography was utilised to isolate TNF-like activity from the culture supernatant. The peak of TNF-like cytotoxic activity corresponded to the fractions with a nlolecular weight of 81 kDa or higher. Polyclonal anti-human TNF-a antiserum cross-reacted by Western blotting with a 17 kDa protein in the TNF-containing fraction ~ ~ n d e denaturing conditions. This result indicated that chicken TNF-like factor in the biologically active form may be a protein multimer of monomers …