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Biochemical and Biomolecular Engineering

Electronic Thesis and Dissertation Repository

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Clostridium beijerinckii

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Full-Text Articles in Engineering

Biobutanol Production From Cellulosic And Sugar-Based Feedstock From The Corn Plant, Reyna Gomez-Flores Apr 2018

Biobutanol Production From Cellulosic And Sugar-Based Feedstock From The Corn Plant, Reyna Gomez-Flores

Electronic Thesis and Dissertation Repository

In this thesis, biobutanol production by biological fermentation was investigated from the corn plant, integrating two approaches. The first one was to utilize corn cobs, a cellulosic-based material. The second, using a new sugar-based material, sugarcorn juice. Utilizing suitable Clostridia strains for each substrate, these approaches converged into a biorefinery concept to produce renewable biofuels in Ontario, Canada. The corn cob pretreatment was carried out by a dilute acid method resulting in temperature as the variable with most significant effect towards glucose liberation. The enzymatic hydrolysis was performed utilizing a very low concentration of an enzymatic stock solution with approximately …


Biohydrogen Production From Cellulose By Clostridium Termitidis And Clostridium Beijerinckii., Maritza Gomez-Flores Sep 2015

Biohydrogen Production From Cellulose By Clostridium Termitidis And Clostridium Beijerinckii., Maritza Gomez-Flores

Electronic Thesis and Dissertation Repository

The purpose of this study was to determine Clostridium termitidis microbial kinetics on glucose, cellobiose, and cellulose, and to assess its co-culture with Clostridium beijerinckii for hydrogen production. Microbial kinetics parameters of the mesophilic, cellulolytic, and hydrogen producer C. termitidis were determined on glucose and cellobiose using MATLAB for modelling biomass growth and substrate consumption. Hydrogen yields on these substrates were 1.99 and 1.11 mol H2 mol-1 hexose equivalent, respectively. C. termitidis microbial kinetics in mono-culture and in co-culture with mesophilic hydrogen producer C. beijerinckii were also investigated under agitated and non-agitated conditions, with hydrogen yields of 1.46 …