Engineering Commons^™

Production Of Recombinant Human Coagulation Factor Ix By Transgenic Pig, Weijie Xu

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Hemophilia B is the congenital bleeding disorder caused by deficiency in functional coagulation factor IX (FIX) and about 28,000 patients worldwide in 2012. And current treatment is restricted to protein-replacement therapy, which required FIX concentrates for patients’ life-time. Approximately 1 billion units FIX were consumed in 2012. However, still about 70-80% patients, mostly in developing countries, received inadequate or no treatment because of the unavailable and/or unaffordable FIX concentrates. Considering safety reasons, e.g. transmission of blood-borne diseases, the recombinant human FIX (rFIX) is recommended other than the plasma-derived FIX. However, only one rFIX is currently available on the market. The …

Pharmacokinetic Characterization Of Procoagulation Proteins, Nicholas C. Vanderslice

Department of Chemical and Biomolecular Engineering: Theses and Student Research

The cessation of bleeding in mammals occurs due to a well-conserved sequence of protein activation known as the coagulation cascade. However, people who have a deficiency in one or more proteins in this cascade, whether due to genetics or blood loss, struggle to maintain hemostasis. In order to aid patients in the restoration of hemostasis, exogenous proteins are often administered in response to bleeding events. However, these proteins are limited and costly due to limited supply of donor blood available for the production of plasma-derived proteins and the high cost of mammalian cell bio-reactors required for the production of recombinant …

Development And Utilization Of A Pair Of Sol-Gel Entrapped Lipases For Biodiesel Production From High Free Fatty Acid Oils, Cory M. Schwartz

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Biodiesel, which consists of fatty acid alkyl esters, is one of the most widely adopted and successful renewable fuels. Traditional physiochemical biodiesel production methods require high cost refined feedstocks, and so alternative methods of catalysis and feedstocks have been explored. This research investigated the use of polysiloxane entrapped lipases to catalyze the production of biodiesel from low cost feedstocks.

In this work, lipase from Burkholderia cepacia (lipase PS) and lipase B from Candida antarctica (CalB) were separately entrapped using sol-gel chemistry. Optimal reaction conditions for the esterification of free fatty acids by immobilized CalB with methanol were determined. Immobilized CalB …