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Protein Sequence-Structure-Function Relationship: Testing Ke-50 Modification On Recombinant Green Fluorescent Protein (Acgfp), Anna E. Burkey
Protein Sequence-Structure-Function Relationship: Testing Ke-50 Modification On Recombinant Green Fluorescent Protein (Acgfp), Anna E. Burkey
Williams Honors College, Honors Research Projects
Protein modification shows promise for improving the effectiveness of protein therapy, including the use of Interferon-beta-1b for the treatment of multiple sclerosis. The addition of a chain of 50 lysine and glutamic acid residues (also called a KE50 modification) was tested on green fluorescent protein (AcGFP) to determine its effect on the bioactivity of the protein. Standard transformation protocol was used to grow the normal and modified proteins in E.Coli, and the bioactivity of the protein was measured using quantum yield and extinction coefficients to determine the reduction in brightness from the native form to the modified form. The …