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Full-Text Articles in Engineering
Vitreoscilla Globin Promoter Cloning And Testing In Escherichia Coli, Lauren J. Coffey
Vitreoscilla Globin Promoter Cloning And Testing In Escherichia Coli, Lauren J. Coffey
Rose-Hulman Undergraduate Research Publications
No abstract provided.
Predicting The Dynamic Binding Capacities For Adsorbents Using Bsa As A Model Protein, Gaoshan Li
Predicting The Dynamic Binding Capacities For Adsorbents Using Bsa As A Model Protein, Gaoshan Li
Rose-Hulman Undergraduate Research Publications
Membrane adsorbers are one alternative to traditional bead-based chromatography that receive attention in the bioseparation process because of their ability to process material with lower residence times. However some properties of membranes make membrane adsorbers unrealistic for some applications. For example, some membrane adsorbers exhibit lower binding capacities compared with the traditional bead-based chromatography counterparts. The dynamic binding capacity (DBC) in this study is defined as the amount of protein bound in the column when the outlet reaches a certain concentration, which is 10% [2], and the equilibrium binding capacity (EBC) is the binding capacity under the equilibrium conditions, when …
Advancement Of Oxygen Biosensor In Escherichia Coli, Caitlyn Meiser
Advancement Of Oxygen Biosensor In Escherichia Coli, Caitlyn Meiser
Rose-Hulman Undergraduate Research Publications
Microbial biosensors can be used to provide information about the cells’ environment in large-scale fermentations. In this project an oxygen sensitive biosensor is being developed in Escherichia coli to determine what kind of conditions cells are growing in: aerobic or anaerobic. This project specifically studies expression from the fumarate and nitrate reductase (FNR) promoter (PFNR) under aerobic and anaerobic conditions. In fluorescence experiments, the expression levels of green fluorescent protein (GFP) were used to quantify the effectiveness of the PFNR in the DH5α, MG1655 and BL21 strains of E. coli. The negative control of the experiments, …
Development And Characterization Of Oxygen Sensitive Microbial Biosensors, Jonathan Lee
Development And Characterization Of Oxygen Sensitive Microbial Biosensors, Jonathan Lee
Rose-Hulman Undergraduate Research Publications
Microbial biosensors are useful in detecting suboptimal conditions in a bioreactor. This detection can help in development of improved mixing methods to reduce the occurrence of these conditions. In this research, the activities of 3 promoters known to have activity under anaerobic conditions, the gadB promoter, an FNR protein activated promoter, and the pfl promoter, were tested for activity under anaerobic conditions by connecting them to a green fluorescent protein (GFP) gene. Using two types of media, Luria-Bertani (LB) and Wilms-Reuss media, the activity of these promoters under aerobic and anaerobic conditions were tested. Results indicate that while the FNR …