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Degradation Of Estrogens In Dairy Waste Solids: Effects Of Acidification And Temperature, D. Raj Raman, Alice C. Layton, Lara B. Moody, James P. Easter, Gary S. Sayler, Robert T. Burns
Degradation Of Estrogens In Dairy Waste Solids: Effects Of Acidification And Temperature, D. Raj Raman, Alice C. Layton, Lara B. Moody, James P. Easter, Gary S. Sayler, Robert T. Burns
D. Raj Raman
Manure–borne estrogens are increasingly recognized as a potential ecological hazard. However, sample–handling protocols for these compounds are not clearly delineated in the literature, nor are comparisons between assays for estrogens. A study was conducted to explore the degradation of estrogen in separated dairy manure waste solids (press cake), using three popular assay types. Estrogens were measured by enzyme–linked immunosorbent assay (ELISA), gas–chromatography mass–spectroscopy (GC–MS) and a recombinant yeast estrogen reporter assay. As measured by GC–MS, background estrone concentrations were approximately 100 ppb, while 17β–estradiol concentrations were one–third of the estrone concentration, and 17β–estradiol concentrations were below the detection limit (10 …