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An Investigation Of The Effect Of Dietary Sulfate On Bile Acid Conjugation And Kinetic Studies Of Bile Acyl Transferase In Rat Liver Microsomes, Ramesh S. Verma

Doctoral Dissertations

This investigation was undertaken to study the effect of varying levels of dietary sulfate on the relative conjugation of ¹⁴C-cholic acid with taurine and glycine in rat liver microsomal preparations with a view to evaluating the factors controlling glycocholic:taurocholic acid (G:T) ratio at the cellular level. Pure microsomal preparations, obtained after centrifuging at high speed, were incubated with the optimum concentrations of ¹⁴C-cholic acid, glycine, taurine, coenzyme A, magnesium sulfate, ATP and sodium fluoride for 90 minutes at 37°. The radioactive cholic acid conjugates fonned were separated with thin layer chromatography and visualized by spraying with phosphomolybdic acid. …