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Acute High-Intensity Exercise And Skeletal Muscle Mitochondrial Respiratory Function: Role Of Metabolic Perturbation, Matthew T. Lewis, Gregory M. Blain, Corey R. Hart, Gwenael Layec, Matthew J. Rossman, Song-Young Park, Joel D. Trinity, Jayson R. Gifford, Simranjit K. Sidhu, Joshua C. Weavil, Russell S. Richardson

Health and Kinesiology Faculty Publications

Recently it was documented that fatiguing, high-intensity exercise resulted in a significant attenuation in maximal skeletal muscle mitochondrial respiratory capacity, potentially due to the intramuscular metabolic perturbation elicited by such intense exercise. With the utilization of intrathecal fentanyl to attenuate afferent feedback from group III/IV muscle afferents, permitting increased muscle activation and greater intramuscular metabolic disturbance, this study aimed to better elucidate the role of metabolic perturbation on mitochondrial respiratory function. Eight young, healthy males performed high-intensity cycle exercise in control (CTRL) and fentanyl-treated (FENT) conditions. Liquid chromatography-mass spectrometry and high-resolution respirometry were used to assess metabolites and mitochondrial respiratory …

Vasodilatory And Vascular Mitochondrial Respiratory Function With Advancing Age: Evidence Of A Free Radically Mediated Link In The Human Vasculature, Soung Hun Park, Oh-Sung Kwon, Song-Young Park, Joshua C. Weavil, Jay R. Hydren, Van Reese, Robert H. I. Andtbacka, John R. Hyngstrom, Russell S. Richardson

Health and Kinesiology Faculty Publications

Recognizing the age-related decline in skeletal muscle feed artery (SMFA) vasodilatory function, this study examined the link between vasodilatory and mitochondrial respiratory function in the human vasculature. Twenty-four SMFAs were harvested from young (35 ± 6 yr, n = 9) and old (71 ± 9 yr, n = 15) subjects. Vasodilation in SMFAs was assessed, by pressure myography, in response to flow-induced shear stress, acetylcholine (ACh), and sodium nitroprusside (SNP) while mitochondrial respiration was measured, by respirometry, in permeabilized SMFAs. Endothelium-dependent vasodilation was significantly attenuated in the old, induced by both flow (young: 92 ± 3, old: 45 ± 4%) …

Mitochondrial Function In Heart Failure: The Impact Of Ischemic And Non-Ischemic Etiology, Song-Young Park, Joel D. Trinity, J. R. Gifford, Nikolaos A. Diakos, Lauren Mccreath, Stavros Drakos, Russell S. Richardson

Health and Kinesiology Faculty Publications

Background

Although cardiac mitochondrial dysfunction is associated with heart failure (HF), this is a complex syndrome with two predominant etiologies, ischemic HF (iHF) and non-ischemic HF (niHF), and the exact impact of mitochondrial dysfunction in these two distinct forms of HF is unknown.

Methods and results

To determine the impact of HF etiology on mitochondrial function, respiration was measured in permeabilized cardiac muscle fibers from patients with iHF (n = 17), niHF (n = 18), and healthy donor hearts (HdH). Oxidative phosphorylation capacity (OXPHOS), assessed as state 3 respiration, fell progressively from HdH to niHF, to iHF (Complex I + …