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Functional, Proteomic And Bioinformatic Analyses Of Nrf2- And Keap1- Null Skeletal Muscle, Lie Gao, Vikas Kumar, Neetha Nanoth Vellichirammal, Song-Young Park, Tara L. Rudebush, Li Yu, Won-Mok Son, Elizabeth J. Pekas, Ahmed M. Wafi, Juan Hong, Peng Xiao, Chittibabu Guda, Han-Jun Wang, Harold D. Scultz, Irving H. Zucker

Health and Kinesiology Faculty Publications

Key points

• Nrf2 is a master regulator of endogenous cellular defences, governing the expression of more than 200 cytoprotective proteins, including a panel of antioxidant enzymes.
• Nrf2 plays an important role in redox haemostasis of skeletal muscle in response to the increased generation of reactive oxygen species during contraction.
• Employing skeletal muscle-specific transgenic mouse models with unbiased-omic approaches, we uncovered new target proteins, downstream pathways and molecular networks of Nrf2 in skeletal muscle following Nrf2 or Keap1 deletion.
• Based on the findings, we proposed a two-way model to understand Nrf2 function: a tonic effect through a Keap1-independent mechanism under basal …

The Effect Of Acute And Chronic Thermotherapy On Type 2 Diabetic Skeletal Muscle Cell Viability And Gene Expression: Pilot Study, Louay Bachnak, Jean Sparks, Xavier F. Gonzales, Daniel Newmire, Felix Omoruyi

International Journal of Exercise Science: Conference Proceedings

Diabetes is a chronic metabolic disease affecting millions of people globally. Type 2 diabetes is associated with insulin resistance or a defective secretion of insulin from the pancreas. The skeletal muscle system accounts for 80% of glucose uptake and is a vital player in healthy aging and muscle mass maintenance. PURPOSE: The purpose of this study was to investigate the effects of thermotherapy on gene expression and cell viability in Type 2 Diabetic skeletal muscle. METHODS: Human skeletal muscle myoblast (HSMM) and Diabetic Type 2 human skeletal muscle myoblast (D-HSMM) (Lonza Inc, Walkersville, MD) were cultured until 90% confluency was, …

Disuse Atrophy Occurs Without A Change In Mitochondrial Respiratory Control Ratio During Hindlimb Unloading In Mice, Indu Reddy Enukonda, Megan E. Rosa-Caldwell, David E. Lee, Jacob L. Brown, Richard A. Perry, Tyrone A. Washington, Nicholas P. Greene, Michael P. Wiggs

International Journal of Exercise Science: Conference Proceedings

Skeletal muscle atrophy commonly occurs during prolonged periods of inactivity, however, the precise mechanisms that cause muscle atrophy have yet to be determined. Specifically, a controversy exists on whether mitochondrial dysfunction is a cause or consequence of disuse muscle atrophy. PURPOSE: The purpose of this study was to determine if a change in the respiratory control ratio, which is a ratio of maximal O₂ respiration to leak respiration, could be detected prior to muscle atrophy in a time-course study in mice. METHODS: Disuse atrophy was induced using hindlimb unloading (HU) in adult, C57BL/6J male mice for 0 …

Exploring The Role Of Bmp7 Gene Expression In An In Vitro Model Of Aging Human Skeletal Muscle., Dreanna M. Mcadams, Emily L. Zumbro, Chase M. White, Ryan A. Gordon, Anthony A. Duplanty

International Journal of Exercise Science: Conference Proceedings

Sarcopenia is an age-related condition associated with rapid loss of skeletal muscle (SKM) tissue that affects mobility and quality of life of geriatric individuals. Mechanistic Target of Rapamycin (mTOR) and Protein Kinase B (AKT) have significant roles in SKM hypertrophy with responses to DNA damage and repair within SKM. However, mTOR and AKT expression is significantly decreased with age. Upstream of AKT, Bone Morphogenetic Protein (BMP7) is a member of the TGF-β signaling family that has been reported as a positive regulator of muscle hypertrophy through the Bmp–Smad1/5/8 signaling axis. PURPOSE: To use an in vitro model of aging …

Ubiquitin Proteasome System Activity Is Suppressed By Curcumin Following Exercise-Induced Muscle Damage In Human Skeletal Muscle, Thomas D. Cardaci, Steven B. Machek, Dylan T. Wilburn, Paul S. Hwang, Darryn Willoughby Phd

International Journal of Exercise Science: Conference Proceedings

PURPOSE: Curcumin is a natural polyphenolic compound with antioxidant and anti-inflammatory properties. In vitro and in vivo animal studies have demonstrated that exposure to curcumin leads to dysregulation of the ubiquitin-proteasome system (UPS). However, to date, no study has investigated curcumin’s ability to influence UPS activity in a human model. Therefore, the purpose of this study was to investigate the effects of curcumin supplementation on markers of UPS activity in the presence of elevations in UPS activity due to exercise-induced muscle damage. METHODS: Twenty-three recreationally active male and female participants between the ages of 18-30 were randomized into …