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Full-Text Articles in Education
Wnt5a-Jnk Regulation Of Vascular Insulin Resistance In Human Obesity, Melissa G. Farb, Shakun Karki, Song-Young Park, Samantha M. Saggese, Brian Carmine, Donald T. Hess, Caroline Apovian, Jessica L. Fetterman, Rosa Breton-Romero, Naomi M. Hamburg, Jose J. Fuster, Maria A. Zuriaga, Kenneth Walsh, Noyan Gokce
Wnt5a-Jnk Regulation Of Vascular Insulin Resistance In Human Obesity, Melissa G. Farb, Shakun Karki, Song-Young Park, Samantha M. Saggese, Brian Carmine, Donald T. Hess, Caroline Apovian, Jessica L. Fetterman, Rosa Breton-Romero, Naomi M. Hamburg, Jose J. Fuster, Maria A. Zuriaga, Kenneth Walsh, Noyan Gokce
Health and Kinesiology Faculty Publications
Obesity is associated with the development of vascular insulin resistance; however, pathophysiological mechanisms are poorly understood. We sought to investigate the role of WNT5A-JNK in the regulation of insulin-mediated vasodilator responses in human adipose tissue arterioles prone to endothelial dysfunction. In 43 severely obese (BMI 44±11 kg/m2) and five metabolically normal non-obese (BMI 26±2 kg/m2) subjects, we isolated arterioles from subcutaneous and visceral fat during planned surgeries. Using videomicroscopy, we examined insulin-mediated, endothelium-dependent vasodilator responses and characterized adipose tissue gene and protein expression using real-time polymerase chain reaction and Western blot analyses. Immunofluorescence was used to quantify endothelial nitric oxide …