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Full-Text Articles in Education
Transitioning Cell Culture Cure Labs From Campus To Online: Novel Strategies For A Novel Time, Jaime L. Sabel, Kendra Wright, Jacob J. Adler, Gary Bates, La Shall Bates, Sumali Pandey, Amanda M. Simons, Sarah J. Swerdlow, Nathan S. Reyna, Lori Hensley
Transitioning Cell Culture Cure Labs From Campus To Online: Novel Strategies For A Novel Time, Jaime L. Sabel, Kendra Wright, Jacob J. Adler, Gary Bates, La Shall Bates, Sumali Pandey, Amanda M. Simons, Sarah J. Swerdlow, Nathan S. Reyna, Lori Hensley
Articles
Course-based undergraduate research experiences (CUREs) provide a way for students to gain research experience in a classroom setting. Few examples of cell culture CUREs or online CUREs exist in the literature. The Cell Biology Education Consortium (CBEC) provides a network and resources for instructors working to incorporate cell-culture based research into the classroom. In this article, we provide examples from six instructors from the CBEC network on how they structure their cell-culture CUREs and how they transitioned the labs to online in the spring semester of 2020. We intend for these examples to provide instructors with ideas for strategies to …
Rapid Verification Of Terminators Using The Pgr-Blue Plasmid And Golden Gate Assembly, Jace C. Bradshaw, Allea Belle Gongola, Nathan S. Reyna
Rapid Verification Of Terminators Using The Pgr-Blue Plasmid And Golden Gate Assembly, Jace C. Bradshaw, Allea Belle Gongola, Nathan S. Reyna
Articles
The goal of this protocol is to allow for the rapid verification of bioinformatically identified terminators. Further, the plasmid (pGR-Blue) is designed specifically for this protocol and allows for the quantification of terminator efficiency. As a proof of concept, six terminators were bioinformatically identified in the mycobacteriophage Bernal13. Once identified, terminators were then made as oligonucleotides with the appropriate sticky ends and annealed together. Using Golden Gate Assembly (GGA), terminators were then cloned into pGR-Blue. Under visible light, false positive colonies appear blue and positively transformed colonies are white/yellow. After induction of an arabinose inducible promoter (pBad) with arabinose, colony …