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The Effects Of Decellularized Adipose Tissue Constructs On Mesenchymal Stromal/Stem Cell Phenotype And Pro-Angiogenic Secretory Function., Yehia Moharrem

Electronic Thesis and Dissertation Repository

Due to limited treatment options for critical limb ischemia (CLI), cellular-based therapies have been investigated to induce blood vessel regeneration. Bone marrow-mesenchymal stromal/stem cells (BM-MSC) have shown pre-clinical success in animal models of CLI as they possess pro-angiogenic and immunomodulatory functions. However, clinical translation has been hindered by inadequate expansion and delivery strategies. This project aimed to characterize the phenotype and pro-angiogenic secretory function of BM-MSC on decellularized adipose tissue (DAT) bioscaffolds as expansion platforms. Compared to cells grown on tissue-culture plastic, DAT substrates supported BM-MSC growth, regenerative marker expression, and pro-angiogenic secretory function. Conditioned media generated by BM-MSC cultured …

The Co-Delivery Of Syngeneic Adipose-Derived Stromal Cells And Macrophages On Decellularized Adipose Tissue Bioscaffolds For In Vivo Soft Tissue Regeneration, Hisham A. Kamoun

Electronic Thesis and Dissertation Repository

Decellularized adipose tissue (DAT) bioscaffolds are a promising platform for the delivery of pro-regenerative cell populations with the goal of promoting adipose tissue regeneration. The current study investigated the effects of seeding DAT bioscaffolds with syngeneic bone marrow-derived macrophages and/or adipose-derived stromal cells (ASCs) on in vivo soft tissue regeneration. Methods were established to derive the macrophages from MacGreen mice, which were dynamically seeded onto the DAT scaffolds alone or in combination with ASCs. Seeded and unseeded scaffolds were implanted subcutaneously into C57Bl/6 mice. At 2 and 4 weeks, cell infiltration, angiogenesis, and adipogenesis were analyzed through histology and immunohistochemistry. …

Functional Co-Substituted Poly[(Amino Acid Ester)Phosphazene] Biomaterials, Amanda L. Baillargeon

Electronic Thesis and Dissertation Repository

The development of new and improved biomaterials is essential for tissue engineering and regenerative medicine applications. Amino acid-based polyphosphazenes are being explored as scaffold materials for tissue engineering applications due to their non-toxic degradation products and tunable material properties. This work focuses on the synthesis of non-functional and novel functional poly[(amino acid ester)phosphazene]s using a facile method of thermal ring opening polymerization followed by one-pot room temperature substitution. The family of polyphosphazenes developed in this work is based on L-alanine (PNEAs), L-phenylalanine (PNEFs), and L-methionine (PNEMs) with L-glutamic acid imparting the functionality. Characterization of these materials demonstrated that the one-pot …

Calcium Phosphate-Based Resorbable Biomaterials For Bone Regeneration, Daniel O. Costa

Electronic Thesis and Dissertation Repository

Bone defects are a prevalent problem in orthopedics and dentistry. Calcium phosphate-based coatings and nanocomposites offer unique solutions towards producing scaffolds with suitable physical, mechanical and biological properties for bone regeneration.

We developed a novel method to synthesize hydroxyapatite (HA) particles with high aspect ratio using sol-gel chemistry and hydrothermal treatment. We obtained tunable pure-phase carbonated-HA in the form of micro/nanorods and nanowires (diameters 25-800 nm). To mimic the structure of bone, HA nanowires were homogenously mixed within poly(ε-caprolactone) (PCL) to produce nanocomposites with improved mechanical properties as determined by uniaxial tensile testing.

Surface chemistry and topography of biomaterials play …