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Design And Synthesis Of Beta-Hairpin Peptidomimetics For Modulating Integrin Mediated Cell Adhesion, Abeta Fibrillogenesis And P53-Mdm2 Protein-Protein Interactions, Priyesh Jain

USF Tampa Graduate Theses and Dissertations

Inhibiting therapeutically important protein-protein interactions has been a tremendous challenge for medicinal chemists. The folded 3D structures of peptides and proteins, mainly comprise secondary structural elements i.e α-helices and β-sheet have created an opportunity to design small molecules and peptidomimetic inhibitors of protein-protein interaction (PPI). Hence, information about the formation and stabilization of these secondary structures is vital for designing future drugs. In this dissertation, several cyclic beta-hairpin peptidomimetics that mimic the recognition surface have been designed and synthesized as inhibitors for different targets such as integrin mediated extracellular matrix -cell adhesion in multiple myeloma, p53-MDM2 PPI, amyloid beta fibrillogenesis …

Advanced Signal Processing Techniques For Single Trial Electroencephalography Signal Classification For Brain Computer Interface Applications, Kun Li

USF Tampa Graduate Theses and Dissertations

Brain Computer Interface (BCI) is a direct communication channel between brain and computer. It allows the users to control the environment without the need to control muscle activity [1-2]. P300-Speller is a well known and widely used BCI system that was developed by Farwell and Donchin in 1988 [3]. The accuracy level of the P300-BCI Speller as measured by the percent of communicated characters correctly identified by the system depends on the ability to detect the P300 event related potential (ERP) component among the ongoing electroencephalography (EEG) signal. Different techniques have been tested to reduce the number of trials needed …

A Framework For Determining The Reliability Of Nanoscale Metallic Oxide Semiconductor (Mos) Devices, Wilkistar Otieno

USF Tampa Graduate Theses and Dissertations

An increase in worldwide investments during the past several decades has pro-pelled scienti c breakthroughs in nanoscience and technology research to new and exciting levels. To ensure that these discoveries lead to commercially viable prod-ucts, it is important to address some of the fundamental engineering and scientific challenges related to nanodevices. Due to the centrality of reliability to product integrity, nanoreliability requires critical analysis and understanding to ensure long-term sustainability of nanodevices and systems. In this study, we construct a relia-bility framework for nanoscale dielectric lms used in Metallic Oxide Semiconductor (MOS) devices. The successful fabrication and incorporation of metallic …

Dna Methylation And Its Association With Prenatal Exposures And Pregnancy Outcomes, Jennifer Straughen

USF Tampa Graduate Theses and Dissertations

Altered DNA methylation may lead to suboptimal fetal programming, increasing the risk of adverse pregnancy outcomes such as small for gestational age (SGA); however, few studies have examined the associations between DNA methylation, prenatal exposures, and fetal outcomes. Cross-sectional data from a larger, ongoing study were used to assess the impact of prenatal smoking on gene specific methylation of umbilical cord blood derived DNA and to investigate the association between gene-specific methylation and risk of SGA. The association between gene-specific DNA methylation and birthweight was also assessed. Maternal and infant covariates were abstracted from medical records, cigarette smoke exposure was …

Evaluation Of Common Inherited Variants In Mitochondrial-Related And Microrna-Related Genes As Novel Risk Factors For Ovarian Cancer, Jennifer Permuth Wey

USF Tampa Graduate Theses and Dissertations

Epithelial ovarian cancer (EOC) is a leading cause of morbidity and mortality among women in the United States, and the etiology is incompletely understood. Common, low penetrant genetic variants such as single nucleotide polymorphisms (SNPs) likely contribute to a significant proportion of EOC. We examined whether SNPs in two understudied yet biologically important types of genes, mitochondrial-related and miRNA-related genes, may contribute to EOC susceptibility using data from a large, homogeneous study population of 1,815 EOC cases and 1,900 controls (frequency-matched on age-group and race/ethnicity) genotyped through stage 1 of an ongoing genome-wide association study. Inter-individual variation in genes involved …

Epidemiological Study Of Contributing Factors In The Development Of Peptic Ulcer And Gastric Cancer Initiated By Helicobacter Pylori Infection In India, Rahul Suresh Mhaskar

USF Tampa Graduate Theses and Dissertations

Background: Helicobacter pylori (H. pylori) infection is a significant risk factor for peptic ulcer (PU) and gastric cancer (GC). Apart from the virulent CagA genotype of H. pylori environmental and dietary factors influence disease outcomes. There have been no studies addressing these factors in Western India. Hence, we conducted a case control study enrolling PU, GC patients and controls at Pune, India.

Methods: Risk factors for PU and H. pylori infection were assessed in participant interview. H. pylori status was assessed from stool by monoclonal antigen detection. To understand treatment effect, we followed 100 H. pylori positive patients.

Results: We …

Corrole Synthesis And Catalytic Applications Toward Cobalt(Iii)-Catalyzed Epoxidation, N-H Insertion, And Cyclopropanation, Chung Sik Kim

USF Tampa Graduate Theses and Dissertations

A variety of bromocorroles, useful precusors for Pd-based cross coupling, were sucessfully synthesized in moderate to good yields. Chiral corroles were also synthesized through use of chiral amides in the aforementioned cross coupling reactions. Cobalt complexes bearing π-acceptor (CNtBu) and σ-donor (PPh3) ligands were also prepared in excellent yields. In this dissertation, cobalt (III) corrole complexes were applied to three different reaction areas: epoxidation, N-H insertion, and cyclopropanation. Cobalt(III) corroles were found to be efficient catalysts for epoxidation reactions. The epoxidation reactions can be carried out using ethyl phenyldiazoacetate, an acceptor/donor diazo compound, as a carbenoid precusor.The reaction provided highly …

Florida's Adolescent Literacy Policy: An Alternative Reading And Response, Diane Kroeger

USF Tampa Graduate Theses and Dissertations

In this qualitative case study, I constructed interpretations of the meanings conveyed by state-level discourse communities as they were manifested in the primary and secondary speech genres of Florida’s adolescent literacy policy. Meanings (or values, beliefs and feelings) are highly tacit understandings embedded in the language, actions and objects of policy (Yanow, 2000), and are conveyed through informal and formal speech (Bakhtin, 1986). Results revealed (a) state policy meanings convey multiple versions of literacy with a heavy focus on receptive aspects of literate practice; (b) a typology of students and their fit within the institutional system; and (c) an emphasis …

Aspects Of The Life History Of The Snowy Grouper, Epinephelus Niveatus, In The Gulf Of Mexico, Kelley Kowal

USF Tampa Graduate Theses and Dissertations

Knowledge of many life history parameters are essential to properly assess and manage fish species. Although the snowy grouper, Epinephelus niveatus, is a commercially valuable fish, which is harvested throughout the Gulf of Mexico, little is known about its age, growth, and reproduction from this region. In this study, snowy grouper from the northern and eastern regions of the Gulf of Mexico were examined primarily using commercially-derived samples that were collected between 1984 and 2004. A total of 1,200 snowy grouper with fork lengths between 242 and 1,190 mm were collected. Sectioned saggital otoliths were used to determine the age …

Discovery Of Novel Viruses From Animals, Plants, And Insect Vectors Using Viral Metagenomics, Terry Fei Fan Ng

USF Tampa Graduate Theses and Dissertations

Understanding emerging viruses is critical for disease monitoring and prediction; however, surveys of novel viruses are hindered by the lack of a universal assay for viruses. Viral metagenomics, consisting of viral particle purification and shotgun sequencing, is a powerful technique for discovering viruses in a wide variety of sample types. However, current protocols are not effective on tissue samples (e.g., lungs, livers and tumors), where they are hindered by the high amount of host nucleic acids which limits the percentage of sequences that originate from viruses. In this dissertation, a modified viral metagenomics protocol was developed and utilized to effectively …

Antecedents And Consequences Of Channel Alienation: An Empirical Investigation Within Franchised Channels Of Distribution, Ivan Lapuka

USF Tampa Graduate Theses and Dissertations

Investigating an important overlooked phase of interorganizational relationship evolution, which is currently hypothesized to progress through five stages of awareness, exploration, expansion, commitment, and dissolution, this dissertation proposes that in the long road between commitment and dissolution, the quintessential interfirm relationship is likely to be characterized by aprolonged period of relationship alienation, which then becomes the immediate precursor to the dissolution stage.

The dissertation utilizes social learning theory, behavior constraint theory, and alienation theory to explain apathetic behaviors of franchisees. The principal proposition is that certain characteristics of the franchise system’s operating environment inadvertently condition franchisee estrangement and failure, and …

Down, But Not Out: An Ethnographic Study Of Women Who Struggled With And Overcame Methamphetamine Addiction, Jodi Nettleton

USF Tampa Graduate Theses and Dissertations

Women suffer methamphetamine (meth) addiction at a rate much higher than rates for addiction to other drugs. Female meth users are susceptible and predisposed to gender-related risks: high rates of unprotected vaginal and anal sex, sex-work, and sexual coercion. Precursors for addiction (e.g., abuse, body dysphasia) put females in a difficult position for recovery and highlight the need for gender-specific research and treatment.

Methamphetamine (a synthetically derived stimulant) creates psychological and physical dependency that affects every neuron of the brain and damages the body immediately. Women ingest meth for initial effects that allay social pressures: feeling euphoric, connecting with others …

African American Athletes And The Negotiation Of Public Spaces: An Examination Of Athletic Capital And African American Perceptions Of Success, Keona Lewis

USF Tampa Graduate Theses and Dissertations

This dissertation explores the culture of sport among African American male football players as well as African American perspectives on sport and success. A case study of six African American, Division 1 FBS (Football Bowl Subdivision) collegiate student athletes was conducted along with seventeen supplemental interviews with community members, parents, coaches and former athletes and fans. The participants answered questions that explored education, success, identity construction, ethnicity and sport. Archival data was also reviewed framing the discussion on football in Florida, links between education and sport participation and African American male academic achievement. While many perspectives varied, there were collective …

Comparison Of Safety Performance By Design Types At Freeway Diverge Areas And Exit Ramp Sections, Hongyun Chen

USF Tampa Graduate Theses and Dissertations

The primary objective of the study is to evaluate the safety performance of different freeway exit types used in current practical designs. More specific, the research objectives include the following two parts: 1) to compare the safety performance of different design types at freeway diverge areas and exit ramp sections; and 2) to identify the impact factors contributing to the crashes happening at these two specific segments.

The study area includes four subjects, the freeway widely-spaced diverge areas; the freeway closely-spaced diverge areas; the left-side off-ramps and the exit ramp sections. For the freeway diverge areas, design types were defined …

A Multi-Level Study Investigating The Impact Of Workplace Civility Climate On Incivility And Employee Well-Being, Raymond Charles Ottinot

USF Tampa Graduate Theses and Dissertations

This study used Zohar‟s (2000) multi-level model of climate to examine the extent to which shared perceptions of workplace civility climate relate to teacher job satisfaction, affective commitment, and counterproductive work behaviors (CWB-abuse) towards other teachers. Workplace civility climate is defined as employee perceptions of how management uses policies, procedures, and practices to maintain a civil workplace. An online-survey was used to assess a cross-sectional sample of K-12 teachers (N = 2222) nested in 207 schools in a large US school district. There was adequate agreement among teacher perceptions of school civility climate for aggregation and between-group variance of civility …

Ambiguous Recognition: Recursion, Cognitive Blending, And The Problem Of Interpretation In Twenty-First-Century Fiction, Christopher David Kilgore

Doctoral Dissertations

This dissertation uses theories of cognitive conceptual integration (as outlined by Gilles Fauconnier and Mark Turner) to propose a model of narrative reading that mediates between narratology and theories of reception. I use this model to demonstrate how new experimental narratives achieve a potent balance between a determinate and open story-form. Where the high postmodernists of the 1970s and 80s created ironic, undecidable story-worlds, the novels considered here allow readers to embrace seemingly opposite propositions without retreating into ironic suspension, trading the postmodernist “neither/nor” for a new “both/and.” This technique demands significant revision of both descriptions of radical experimentation in …

Composite Of Complexity: Manifestations Of Whiteness And Class Among Las Vegas Italian Americans, Danielle Nicole Axt

UNLV Theses, Dissertations, Professional Papers, and Capstones

Conventional terminology and conventional boundaries, in regard to ethnicity, are no longer applicable to the ever shifting population of the United States. In specific, the various degrees of White ethnic identity does not easily transition into convenient all encompassing categories such as Caucasian or more simply, White. Whiteness studies have been at the forefront of this critique, most recently asserting that White ethnic identity is heavily influenced by context. However, although many studies are now recognizing the impact of multiple layers of White ethnic identity (along the lines of gender, locale, socioeconomic level etc.) many still neglect to identify a …

Your Presence Is Requested At Suvanto, Maile Chapman

UNLV Theses, Dissertations, Professional Papers, and Capstones

This creative dissertation is a novel set in a fictional private hospital on the southwestern coast of Finland. The main character, Sunny Taylor, is an American nurse whose loneliness and isolation give the novel its distant emotional atmosphere and outsider’s perspective on life in Finland (a densely forested country long perceived as linguistically, culturally, and geographically remote from the rest of Europe). Other main characters include a reserved, chronically ill Finnish woman born before independence from Russia and educated as an architect; an unpleasant expatriate Danish woman who once gave ballroom dance lessons in Finnish cafés; an American obstetrician who …

Must Pay Now, David C. Perkins

UNLV Theses, Dissertations, Professional Papers, and Capstones

These poems attempt to stand amidst the towering shadows of Enlightenment. One of these pillars involves the newfound land from a collective western European vantage and these lands are called the Americas. This space is where these poems are located. They suckle at the monolithic breasts of Enlightened Romance as did Romulus and Remus to the She-Wolf. The poems in their own originality engage with writers such as Jonathan Edwards, Alice Notley, Susan Howe, Frank O’Hara, William Carlos Williams, Walt Whitman, Christina Rossetti, William Blake, and John Cage. If there ever was such a thread in tradition, these people might …

History And Transnational Identities In Junot Díaz’S The Brief Wondrous Life Of Oscar Wao, Brian Joseph Flores

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of my thesis is to analyze Junot Díaz’s The Brief Wondrous Life of Oscar Wao and evaluate the role literature plays within the larger context of the relationship among the different countries and cultures in the Western Hemisphere, as well as the place historical events play within this understanding. In Díaz’s novel, there is an understanding of the presence of multiple cultural identities. This awareness of multiple cultural identities leads to the difficulty the characters encounter when trying understanding themselves as individuals. On a much larger scale, the characters also try to understand their cultural, social, and historical …

The Crooked Median, Monica Zarazua

UNLV Theses, Dissertations, Professional Papers, and Capstones

Words search. There are specific points designated by written language, where one might stand for just a little while until the satisfaction of a pattern is revealed. In this collection of stories, one of the forces that serves as a catalyst for this search is the outside gaze. The gaze exerts itself onto characters. The characters may or may not be conscious of it, may or may not welcome it, but they must grapple with it. The gaze projects its needs and desires onto the characters. It seeks to control them, and it desires to be viewed with admiration, lowered …

The Noble Savage And Ecological Indian: Cultural Dissonance And Representations Of Native Americans In Literature, Brooke D. Mcnaughton

Undergraduate Honors Capstone Projects

This thesis provides a unique approach to understanding the historical origins and contemporary social ramifications of the use of the concepts of the Noble Savage and the Ecological Indian within literature. I first examine the history of the Noble Savage concept in literature by examining relevant social movements, and then its eventual transition into its modern counterpart, the Ecological Indian. Authors who employ the use of these concepts typically portray Natives in a way which provides an idealized alternative for white cultural woes. Consequently, this idealization creates problems with modern Native identity. In the second half of this project I …

"Their Past In My Blood": Paule Marshall, Gayl Jones, And Octavia Butler's Response To The Black Aesthetic, Williamenia Miranda Walker Freeman

Dissertations

Paule Marshall’s The Chosen Place, The Timeless People (1969), Gayl Jones’ Corregidora (1975), and Octavia Butler’s Kindred (1979) enhance our conceptualization of black aestheticism and black nationalism as cultural and political movements. The writers use the novel as genre to question the ideological paradigm of a black nationalist aesthetic by providing alternative definitions of community, black women’s sexuality, and race relations. Because of the ways in which these writers respond to black aestheticism and black nationalism, they transform our understanding of movements often perceived as sexist, racist, homophobic, and anti-Semitic. An examination of their works reveals the need for additional …

Before The Storm: Evacuation Intention And Audience Segmentation, Homer J. Rice

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to describe the predictors of evacuation intention among coastal residents in the State of Florida and to determine if there are meaningful segments of the population who intend to evacuate when told to do so by governmental officials because of a major hurricane. In the America’s and the Caribbean, 75,000 deaths have been attributed to hurricanes in the 20

th century. A well planned evacuation can reduce injury and death, yet many people do not have an evacuation plan and do not intend to evacuate when told to do so. The study used secondary …

The Economically Important Nitrogen Pathways Of Southwest Florida, Elon M. Malkin

USF Tampa Graduate Theses and Dissertations

The global phenomenon of burgeoning coastal population growth has led to coastal watershed landscape transformation and ecosystem degradation, prompting policy-makers to set limits on freshwater withdrawals and labile nutrient loads. Important components of Florida’s economies lie in the state’s expansive coastal zone; the organisms driving the billion-dollar recreational fishing industry are rooted in coastal habitats, while the agriculture and real-estate industries sprawl throughout numerous coastal watersheds. This study aimed to identify the connections between anthropogenic land use and essential juvenile fish nursery habitats within the coastal zone, which is the first critical step for sustaining the ecology and related economies …

Modern Variation In Predation Intensity: Constraints On Assessing Predator-Prey Relationships In Paleoecologic Reconstructions, James Funderburk

USF Tampa Graduate Theses and Dissertations

The complex interaction between predators and their prey is rarely preserved in the fossil record. However, predation of marine mollusks by drilling gastropods leaves a diagnostic hole in the shell of the prey, possibly allowing for quantitative analysis of this ecological interaction. Drilling frequency, as measured in marine mollusks both in the Modern and fossil record, has been heralded as a potential opportunity to quantify these ecological interactions and use these values in the testing of hypotheses.

This study employed the collection, tallying, and analysis of bulk samples derived from shelly deposits on 45 Modern beaches along the contiguous coast …

Comparative Study Of Intentional Communities, Jessica Merrick

USF Tampa Graduate Theses and Dissertations

Moving to Florida to retire has become commonplace among American elders, though we have seldom addressed how lesbians and gay men navigate sexual identity in retirement. I undertake ethnography of three suburban, retirement-aged residential communities in Florida in which lesbians and gay men make community in order to understand how identities are produced by and within communities, the significance of suburban gay communities in the post-gay community era, and how residents from each community engage dominant discourse. Sanctuary Cove

1 is a ―gay and lesbian‖ retirement community; Bayside Park is a ―women‘s-only‖ (lesbian) community; and Heritage Estates is a heteronormative …

Chromatin Unfolding By Cdt1 Regulates Mcm Loading Via Opposing Functions Of Hbo1 And Hdac11-Geminin, Philip G. Wong

USF Tampa Graduate Theses and Dissertations

The efficiency of metazoan origins of DNA replication is known to be enhanced

by histone acetylation near origins. Although this correlates with increased MCM

recruitment, the mechanism by which such acetylation regulates MCM loading is

unknown. We show here that Cdt1 induces large-scale chromatin decondensation that is

required for MCM recruitment. This process occurs in G1, is suppressed by Geminin, and

requires HBO1 HAT activity and histone H4 modifications. HDAC11, which binds Cdt1

and replication origins during S-phase, potently inhibits Cdt1-induced chromatin

unfolding and re-replication, suppresses MCM loading, and binds Cdt1 more efficiently

in the presence of Geminin. We also …

Design, Synthesis & Biological Activity Of Novel Protein Tyrosine Phosphatase (Ptp) Mimetics, Sridhar Reddy Kaulagari

USF Tampa Graduate Theses and Dissertations

Protein phosphorylation is a post translational modification of proteins in which a serine, a threonine or a tyrosine residue is phosphorylated by an enzyme, kinase. Phosphorylation of proteins is a reversible and very important regulatory mechanism that occurs in both prokaryotes and eukaryotes. Phosphorylation turns many protein enzymes on and off, preventing or causing many diseases such as diabetes, cancer and rheumatoid arthritis. The phosphorylation on tyrosine residues of proteins is essential for transmission of signals for cell growth, proliferation and differentiation. Protein tyrosine phosphatases (PTPs) in concert with protein tyrosine kinases (PTKs) regulate many signal transduction pathways by controlling …

New Peptide-Pair Screening Strategy And Peptidylglycine A-Hydroxylating Monooxygenase (Phm) Based Enrichment Method For The Discovery Of Novel A-Amidated Peptides, Zhenming An

USF Tampa Graduate Theses and Dissertations

Peptide a-amidation is known as a signature of bioactivity due to the fact that half of the bioactive peptides found in the nervous and endocrine systems are a-amidated and that most known a-amidated peptides are bioactive. a-Amidated peptides are produced by the oxidative cleavage of glycine-extended precursors. Peptidylglycine a-amidating monooxygenase (PAM) is the only known enzyme responsible for catalyzing this reaction and its sole physiological function is to convert glycine extended prohormones to their a-amidated forms. High levels of PAM are found in certain tissues with no corresponding level of amidated products suggesting the presence of undiscovered a-amidated peptide hormones. …