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Theses/Dissertations

2008

Earth Sciences

AC/TC Ratio

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The Mobility Of Fecal Indicator Microorganisms Within A Karst Groundwater Basin In The Inner Bluegrass Region, Kentucky, James Wade Ward Jan 2008

The Mobility Of Fecal Indicator Microorganisms Within A Karst Groundwater Basin In The Inner Bluegrass Region, Kentucky, James Wade Ward

University of Kentucky Doctoral Dissertations

This project implemented novel approaches to assess the source, age, concentration and mobility of fecal indicator microorganisms within a karst groundwater system. Research was conducted in the well-characterized Blue Hole Spring karst groundwater basin in Versailles, Woodford County, Kentucky. At this site the AC/TC ratio and fecal coliform (FC) bacteria counts were used to delineate sources of fecal inputs and determine relative age of the fecal matter. An aging experiment using indicator bacteria (total coliform (TC) and atypical colonies (AC)), which approximated subsurface conditions, indicated that changes in the AC/TC ratio are likely to be retarded during bacterial transport through …