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The Mechanism Of The Fixation Of Tissue Components By Osmium Tetroxide, Robert Barry Litman

Yale Medicine Thesis Digital Library

Virtually all electron density observable in membranous elements in thin sections of buffered OsO4 -fixed tissue with electron microscopy was removed by the action of K4Fe(CN)6 in acetic acid. This reagent reacted only with Os(VIII) and not with lower oxidation states of osmium to give an insoluble crystalline precipitate, K2Fe0s(CN)6. The osmate esters originally synthesized by Criegree (8,9) were unreactive with acetic ferrocyanide. Acetic acid alone decreased membrane density of fixed sections but did not remove osmium from osmate esters.

Further, fresh tissue incubated in a saturated urea solution, then fixed in buffered OsO4 not only failed to blacken, as …

Penicillin Allergy And Hypersensitivity Fever, Michael Joseph Chusid

Yale Medicine Thesis Digital Library

[From the Summary] Rabbits immunized to benzylpenicillin G were found to develop circulating anti-benzylpenicillin antibodies. After immunization, challenge of these animals with intravenous aqueous penicillin was ineffective in eliciting fever. However, intravenous challenge with a penicillin-rabbit serum protein conjugate led to typical hypersensitivity fevers which were roughly correlated in magnitude with titers of benzylpenicillin antibody. Febrile tolerance developed after one or two challenges with the conjugate. This form of hypersensitivity appeared to be transferable with plasma of immunized donors to normal rabbits. Finally, blood leukocytes of immunized rabbits incubated with penicillin-protected conjugate and hypersensitive serum released pyrogen in vitro while …

Metabolic Acidosis And Respiratory Compensation In Uremia During Hemodialysis, Robert D. Gilbert

Yale Medicine Thesis Digital Library

[From the Introduction] In chronic renal insufficiency one of the products of metabolism which accumulates in the body is the hydrogen ion. The phosphoric, sulfuric, and organic acids usually eliminated in the urine accumulate. The respiratory system can and does decrease the hydrogen ion content of the body by decreasing the amount of carbonic acid present. The accumulating hydrogen ions also react with various bases throughout the body. The net result of metabolic production, respiratory elimination, and body buffering is reflected in the blood as chronic, partially compensated, metabolic acidosis. As expected the carbonic acid and block buffer concentrations are …

Sharing: The Dying Patient, His Family, And Those Caring For Him., Bruce Fabric

Yale Medicine Thesis Digital Library

The abstract for this thesis is unavailable at the moment.