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Proteome Characterizations Of Microbial Systems Using Ms-Based Experimental And Informatics Approaches To Examine Key Metabolic Pathways, Proteins Of Unknown Function, And Phenotypic Adaptation, Suresh Poudel
Doctoral Dissertations
Microbes express complex phenotypes and coordinate activities to build microbial communities. Recent work has focused on understanding the ability of microbial systems to efficiently utilize cellulosic biomass to produce bioenergy-related products. In order to maximize the yield of these bioenergy-related products from a microbial system, it is necessary to understand the molecular mechanisms.The ability of mass spectrometry to precisely identify thousands of proteins from a bacterial source has established mass spectrometry-based proteomics as an indispensable tool for various biological disciplines. This dissertation developed and optimized various proteomics experimental and informatic protocols, and integrated the resulting data with metabolomics, transcriptomics, and …
Genetic Diversity, Genetic Variation And Identification Of Quantitative Trait Loci (Qtl) Associated With Biomass Yield And Establishment-Related Traits In Lowland Switchgrass (Panicum Virgatum L.), Cheryl Ontolan Dalid
Genetic Diversity, Genetic Variation And Identification Of Quantitative Trait Loci (Qtl) Associated With Biomass Yield And Establishment-Related Traits In Lowland Switchgrass (Panicum Virgatum L.), Cheryl Ontolan Dalid
Doctoral Dissertations
Switchgrass is a warm-season C4 grass used for biofuel production. The primary goal of this study is biomass yield improvement for use as a bioenergy feedstock. The research plan was partitioned into three main objectives: (i) evaluate the genetic diversity among lowland switchgrass populations using microsatellite markers; (ii) assess genetic variation in an Alamo half-sib (AHS) population developed through phenotypic selection; (iii) and identify quantitative trait loci (QTL) associated with biomass yield and establishment related seed traits using a Nested Association Mapping (NAM) population. The genetic diversity study on lowland switchgrass showed significant phenotypic variations (P<0.05) among and within germplasm accessions. The Analysis of Molecular Variance (AMOVA) revealed that among genotypes within populations and among populations explained 84 and 16% of molecular genetic variations. The Principal Coordinate Analysis (PCoA) and cluster analysis separated switchgrass populations according to the ancestral background. The study on genetic variation showed significant variation (P<0.05) among AHS for biomass yield, tillering ability, and spring vigor, suggesting the importance of additive genetic variation in these traits. Results also showed great potential for biomass yield improvement through selection based on family performance. Using 10% selection intensity, parental control of two, and a narrow-sense heritability estimate of 0.11, gain per cycle selection from half-sib family selection is estimated to be 23%. The study on identification of QTLs associated with biomass yield and establishment related traits showed significant variation among NAM families and genotypes within families for biomass yield, seed weight, and germination (P<0.05). A total of 785 significant markers for biomass yield were detected (P<0.05) of which 176 were for seed weight and 131 for germination. Composite interval mapping revealed 21 QTLs for biomass yield across locations in 2 years the highest LOD score of 7.6 detected in chromosome Ia that explained a 12.5% of the phenotypic variation. Two QTLs located in chromosome VIIIb for seed weight explained 5.2% and 4.6% phenotypic variation. One QTL for germination located in chromosome IXa explained 5.2% of the phenotypic variation. The results of these studies will be useful for future breeding efforts in switchgrass and other perennial grasses.
Identification, Characterization, And Genetic Comparison Of Fusarium Species Isolated From Switchgrass (Panicum Virgatum L.), Tamara Annette Collins
Identification, Characterization, And Genetic Comparison Of Fusarium Species Isolated From Switchgrass (Panicum Virgatum L.), Tamara Annette Collins
Masters Theses
Fusarium spp. are ubiquitous, soilborne and seedborne pathogens that affect over 100 economically important plant hosts annually, resulting in billions of dollars in economic losses. They cause a variety of plant disease symptoms including, crown, root, and stem rots, wilts, and foliar necrosis. Furthermore, they produce harmful mycotoxins that are detrimental to the health of animals and humans. Members of this genus have been isolated from diseased tissues of switchgrass. The objective is to correctly identify and classify Fusarium isolates to species using both classic phenotypic characterization and current genetic technologies of DNA extraction and polymerase chain reaction and create …