Digital Commons Network^™

A Study Of The Urban Red Fox (Vulpes Vulpes) Population In Baton Rouge, Louisiana Using Social Media, Ahsennur Soysal

LSU Master's Theses

Foxes are timid yet resourceful animals that are integrated into many urban environments. Because they are elusive, collecting information about the number of urban foxes, their diet and spatial distribution, their interactions with the ecological community in their urban habitat, as well as residents’ response to them, is difficult. Involving stakeholders to participate in the data collection on wildlife via citizen science on social media is one way to overcome this complication, while simultaneously engaging residents in the ecology happening around them. Therefore, we used social media as the platform to engage the public to document and map the foxes …

Growth And Development Of Two Broiler Strains With Low Protein And Crystalline Amino Acid Supplemented Diets, Chaoyang Li

LSU Master's Theses

The objective of this research was to compare the growth performance of broilers from two commercial breeds with control, low protein and low protein supplemented with crystalline amino acids diets. This was a randomized block design, and identical experiments were conducted on successively in two years. In each experiment, day-old chicks, Ross 708 broilers and Cobb 405 broilers, were randomly assigned into three dietary treatments: 1) positive control, 2) low crude protein (LP), and 3) LP + crystalline amino acids (CAA). A three phase feeding program was used. Feed and water were provided ad-libitum. On d 12, 19, 26, 33, …

Patterns In Abundance Of Louisiana’S Avifauna Revealed By The Louisiana Bird Atlas Project, Matthew L. Brady

LSU Master's Theses

The Louisiana Bird Atlas Project is a multi-year citizen science effort designed to quantify bird distribution and abundance for all species regularly occurring in the state of Louisiana during two time periods: summer and winter. Data were collected by volunteers in a citizen science framework across eight years, from 2007 to 2014. A total of 351 species was recorded (254 during the Summer Bird Atlas and 315 during the Winter Bird Atlas). Maps showing distribution and relative abundance for each species were generated within a Geographical Information System, and were accompanied by short descriptions of status, distribution, abundance, and other …

Occurrence Of Putative Endornaviruses In Non-Cultivated Plant Species And Characterization Of A Novel Endornavirus In Geranium Carolinianum, Rachel Herschlag

LSU Master's Theses

Endornaviruses are RNA viruses, which can infect plants yet cause no apparent symptoms. To date, most descriptions of endornaviruses infecting plants have been in cultivated species. A survey for endornaviruses in non-cultivated plants was initiated in 2015 and continued through 2017 in Baton Rouge, Louisiana. Two hundred and seven plant species were tested for distinctive dsRNA profiles by selective extraction and gel electrophoresis, of which seven contained endornavirus-like dsRNA. RT-PCR amplification of an endornavirus-specific sequence supported the endornavirus nature of six of the seven samples. Of the six host species, one species, Geranium carolinianum was confirmed as being infected with …

Distinguishing Isolates Of Rotylenchulus Reniformis Endemic In Louisiana On The Basis Of Root-Associated Females And Egg Masses, Benjamin Mcinnes

LSU Master's Theses

The reniform nematode Rotylenchulus reniformis is a major pathogen of soybean and cotton in Louisiana. Previous studies have shown that populations of reniform nematode throughout the southern United States vary in reproduction and pathogenicity. Limited studies have been conducted to evaluate the reproduction and pathogenicity of populations of R. reniformis endemic in Louisiana. Studies with isolates of the nematode from eight cotton-producing parishes focused solely on reproduction of the root-associated infective and swollen female life stages with and without attached egg masses on the cotton genotypes MT2468 Ren3, M713 Ren5, and Stoneville 4946GLB2 and the soybean genotypes PI 548316, PI …

Characterization Of Aspergillus Flavus Soil And Corn Kernel Populations From Eight Mississippi River States, Jorge A. Reyes Pineda

LSU Master's Theses

Aspergillus flavus is a saprophytic ascomycete that can also actively invade the seed of crops and potentially contaminate them with harmful aflatoxins. Management of A. flavus currently relies mostly on biocontrol. However, there is still a lot to learn about its biology and ecology so the current approach can be improved. An A. flavus population survey was undertaken during the fall of 2014 across eight Mississippi River states to determine population diversity and geographic distribution of VCGs. Isolates from corn and soil were also characterized for VCG, sclerotial morphotype, mating type, cyclopiazonic acid, and aflatoxin production in order to determine …

The Impact Of Co2-Related Ocean Acidification On The Molecular Regulation Of Shell Development In The Eastern Oyster (Crassostrea Virginica)., Mackenzie L. Richards

LSU Master's Theses

Eastern oysters (Crassostrea virginica), native to the Gulf of Mexico, are keystone species in estuarine ecosystems and are economically valued. Current research indicates that ocean acidification adversely affects the physiology and morphology of larval oysters, but the molecular mechanisms of this impact remain unstudied. Ocean acidification is contributed to by elevated atmospheric CO₂ due to increased anthropogenic activities, causing heightened partial pressure of CO₂ (pCO₂), and eutrophication from land-based runoff in the Gulf. The objective of this work was to determine the genomic response of the eastern oyster in Louisiana to simulated ocean acidification. …

Effects Of Soil Silicon Amendment And Nitrogen Levels On Rice Insect Pest Complex, James Michael P. Villegas

LSU Master's Theses

Rice plants are consistently subjected to various pressures by insect pests throughout the growing season. The main insect pest complex reported in Louisiana are the rice water weevil (major early-season insect pest), fall armyworm (sporadic early-season pest), sugarcane borer and rice stalk borer (long- established but sporadic stem boring pests), Mexican rice borer (an invasive stem-boring pest), and rice stink bug (major late-season pest). Soil silicon amendment has been shown to enhance plant resistance against herbivorous pests. Rice is a typical silicon-accumulating graminaceous crop. Field and greenhouse experiments were conducted from 2015 to 2017 to investigate the effects of soil …

Effect Of Hot Water Conditioning On Microbial Safety And Quality Of In-Shell Pecans, Karuna Kharel

LSU Master's Theses

Hot water conditioning of in-shell pecans is one common practice followed by industries to facilitate shelling. It also acts as a preventive control for potential microbiological contamination that might occur during pre- and post-harvest processes. However, heat treatment may have an effect on the eating quality of nuts. The main objectives of the study were to develop a post-harvest hot water treatment intervention as a kill step to destroy foodborne pathogens on in-shell pecans and evaluate the effect of treatments on physicochemical properties, consumer acceptance and purchase intent of dehulled and roasted pecans. The time (1-5 min) and temperature (70, …

The Characterization Of Alpha-Carbonic Anhydrases Through Mutagenesis And Subcellular Localization In Arabidopsis Thaliana, Lance E. Pounds

LSU Master's Theses

Carbonic anhydrases (CAs) are zinc metalloenzymes which catalyze the interconversion of carbon dioxide and bicarbonate. It is hypothesized that CA activity facilitates CO₂ flux in leaf mesophyll cells to maintain optimal rates of photosynthesis or to maintain a pool of inorganic carbon for other metabolic processes. There are three classes of CAs (α, β, and γ) in the model plant Arabidopsis thaliana. The βCAs and γCAs have been well studied, but the αCAs have received relatively little study. There are eight αCA genes in Arabidopsis. This study investigates which αCAs are expressed in shoots, their tissue-specific expression, their …

Incidence Of Aphelenchoides Besseyi In Rice In Louisiana And Host Status Of The Most Widely Planted Cultivars, Felipe Mendes Carvalho Godoy

LSU Master's Theses

Aphelenchoides besseyi, the causal agent of white tip disease of rice, has been considered a minor pest of rice during the past 50 years in the United States. Recently this nematode has been found in a number of quarantine samples in Louisiana and Arkansas. Objectives of this research were to determine incidence of this nematode in commercial seed sold to producers in Louisiana and to determine the host status of major cultivars currently produced in the state. During 2015-2016, a total of 216 seed samples representing 3 medium grain, 18 long grain, and 4 long grain hybrid cultivars were …

Heat, Acid And Osmotic Stress Tolerance Of Leuconostoc Mesenteroides As Influenced By Prior Exposure To Various Mild Stress Conditions, Ingrid C. Osorio Rodriguez

LSU Master's Theses

The importance of Leuconostoc mesenteroides is recognized for its contribution to taste in cultured dairy products. It has been cited for its potential role as a probiotic. Consumer demand exists for new dairy products with health benefits. In the manufacture of probiotic-products survival of Leuconostoc mesenteroides would depend on its ability to tolerate challenging processing and storage conditions. Improved viability by prior exposure to mild stresses can increase stress tolerance toward a more severe stress. Furthermore, it can result in cross-protection due to connection of several proteins in response to stresses. Objective was to evaluate the effect of prior …

Evaluation Of Winter Management Methods To Enhance Survival Of The Giant Salvinia Weevil, Cyrtobagous Salviniae, Lori Robyn Moshman

LSU Master's Theses

Giant salvinia (Salvinia molesta Mitchell) is a noxious aquatic weed in the southeastern United States. Dense plant mats create ecological and economic consequences by displacing native species and impacting freshwater industries. Biological control programs using the salvinia weevil (Cyrtobagous salviniae Calder and Sands, Coleoptera: Curculionidae) have limited efficacy in giant salvinia’s northernmost range due to the weevil’s low cold tolerance and long-term establishment rates. Spunbonded polypropylene (SBP) fabrics and manipulation of plant density were evaluated as two strategies for managing giant salvinia mats during winter. Greenhouse, laboratory, and field experiments were conducted to determine the suitability of these …

Multigeneration Effects Of Chronic Exposure To Polycyclic Aromatic Hydrocarbons In Gulf Killifish (Fundulus Grandis), Andrea Yammine

LSU Master's Theses

The goal of this study was to examine potential adaptation within two generations of Gulf killifish exposed to polycyclic aromatic hydrocarbons (PAH) while also investigating the use of mucus as an effective and non-lethal alternative to determine concentrations of 11-ketotestosterone (11-KT) and vitellogenin (VTG) of F. grandis. Multiple biomarkers were used to assess adaptation within these fish, such as lysozyme (enzyme critical in innate immunity), and 11-ketotestosterone and vitellogenin (endocrine hormones often affected by PAHs).

Mucal concentrations of 11-KT and VTG were determined and evaluated for effectiveness through comparative assays with blood plasma. This method was then applied to …

Interaction Of Cocoa Powder With Intestinal Microbiota, Martha M. Escoto Sabillon

LSU Master's Theses

Cocoa is the fully fermented and dried seed of the cacao tree (Theobroma cacao L.) which has prebiotic properties, due to their high concentration of polyphenols. Therefore, the ingestion of cocoa could cause changes in the proportions of the intestinal microbiota that can influence the intestinal immune response. The objective of this study was to determine the effect of alkalization process of the cocoa bean in the diversity of the gut microbiota. The samples were “lavado” unprocessed cocoa powder, “natural” unprocessed cocoa powder, “D-11-S” as alkalized cocoa powder, “D-11-B” heavily alkalized cocoa powder, and raw cocoa “shells” and a control …

Giant Salvinia, Salvinia Molesta (Salviniaceae): Evaluation Of Sub-Optimum Temperatures On Survival Of The Giant Salvinia Weevil, Cyrtobagous Salviniae (Coleoptera: Curculionidae) And Integration Of Management Practices With Aquatic Herbicides, Lauren W. Cozad

LSU Master's Theses

Salvinia molesta is one of the world’s worst aquatic weeds and has cost Louisiana nearly $7 million worth of damage and economic impact each year. While aquatic herbicides and the biological control agent, Cyrtobagous salviniae, are the most efficacious control methods, there are limitations with each technology. Therefore, studies were conducted to evaluate insect mortality at upper and lower lethal temperatures and investigate integrated pest management (IPM) with insects and herbicides. Three Louisiana populations of C. salviniae were tested to evaluate cold tolerance and found that at 0°C, the Bayou Nicholas population was 1.3- and 1.4-times more cold tolerant …

Impact Of Preemergent Herbicides, Mowing Height, And Fertilization On Centipedegrass Root Architecture And Drought Tolerance, Mike J. Adams

LSU Master's Theses

Mowing, N fertilization and the application of preemergence herbicides (PRE) are cultural practices performed on centipedegrass to improve overall turf quality. It is posited that increasing mowing height and application of fertilizers can lead to greater turfgrass rooting and increase drought survival; while the application of PRE is reported to potentially have negative impacts on rooting. At two locations in Louisiana, studies examining the effects of these practices on centipedegrass rooting and drought tolerance were conducted.

In the first 11-week study conducted, centipedegrass was treated with dithiopyr, pendimethalin, prodiamine, simazine, or indaziflam at the manufacturers’ labeled rates. During the 11-wk …

Optimization Of Overhead Irrigation In Relation To Irrigation Water Use Efficiency In The Louisiana Nursery Industry, Mark A. Wilson

LSU Master's Theses

Irrigation efficiency is an essential component of nursery production in the United States. To increase productivity of Louisiana growers, a series of studies were conducted to evaluate current nursery water practices. The objectives were to determine current irrigation practices; evaluate actual water usage; and compare nursery media for water utilization. A mailed survey determined that the majority of growers used can yard production (63%), overhead irrigation (79%), had no irrigation manager (82%) and did not collect/reuse water(61%); 50% do not test/calibrate their irrigation system. Based on this information, five nurseries using overhead irrigation were selected to determine water application and …

Modifiable And Non-Modifiable Factors Related To The Macular Pigment Optical Density (Mpod) In A Population Of College-Aged Adults, Genesis Gisselle Guerra Gaitan

LSU Master's Theses

Age-related macular degeneration (AMD) is one of the leading causes of blindness among the elderly worldwide. Retinal problems, specifically in the macula, hold potential for development of AMD. Macular pigment optical density (MPOD) gives a measure of the thickness of the macula and therefore health of the macula. Some of the populations at risk for development of AMD include being white, female, and having light eye color. Factors related to the development of the disease have been divided into modifiable and non-modifiable; non-modifiable include a genetic predisposition. Control of modifiable factors, including body mass index (BMI) and diet [dietary intake …

Impact Of Pre-Bloom Square Loss On Yield And Lint Quality In Louisiana Cotton Cropping Systems, Cory L. Cole

LSU Master's Theses

During the 2016 growing season, research was conducted at three locations in the state of Louisiana to evaluate the impact of pre-bloom square loss on cotton lint yield and fiber quality. Two cotton varieties, Phytogen 499 WRF and Phytogen 222 WRF were chosen up which to imitate early season square loss due to tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) and/or cotton fleahopper, Pseudatomoscelis seriatus (Reuter) feeding or unfavorable weather conditions. Thirty plants within each plot were selected and squares were counted. Squares were assigned numbers, and numbers were then randomized using a computerized number generator. To simulate intervals …

Effects Of Elicitor Induced Host Plant Resistance On Lepidopteran Insecticide Efficacy, Abigail Cox

LSU Master's Theses

Soybean looper (SBL), Chrysodeixis includens (Walker), is an important defoliating Lepidopteran pest of southern U.S. soybean and utilizes other agronomic crops and weeds as hosts. With increasing resistance to insecticides, alternative control strategies such as induced host plant resistance were evaluated against SBL. Jasmonic acid (JA) is an elicitor of host plant resistance, and was selected to determine its fit in an IPM plan for SBL. JA was applied to the top of meridic SBL diet and fed to SBL; no effects were found. JA applied as an exogenous elicitor to cotton, sweet potato, okra, cowpea, and soybean did result …

A Comparison Of Soils And Their Associated Microbial Communities As Affected By Sugarcane Cultivation, Adam Francis Bigott

LSU Master's Theses

In Louisiana, sugarcane has been grown in the same soils for over 200 years. A phenomenon wherein soils with a long-term sugarcane cropping history produce decreased yields compared to adjacent land without a recent history of sugarcane cultivation has been documented in multiple sugarcane growing regions. Research in both Louisiana and internationally has shown positive plant growth responses when soils with a long-term cultivation history are sterilized or treated with selective biocides, suggesting there is a biological component to the underlying soil health issue. In this study, soil microbial ecology was compared for paired sites with short and long-term sugarcane …

Impacts Of Coastal Acidification On Louisiana Plankton, Amy Mallozzi

LSU Master's Theses

Anthropogenic input of carbon dioxide (CO₂) has an increasingly acidifying effect on the world’s oceans with the potential to impact biogeochemical cycles and food web dynamics. Coastal Louisiana is an area highly vulnerable to changing climate, but the unique local water chemistry created by input from the Mississippi and Atchafalaya Rivers makes the manifestation of elevated CO₂ and its biological implications difficult to predict. Louisiana estuaries are highly productive and support a large commercial market for the eastern oyster, Crassostrea virginica. This study explores the use of experimental microcosms to expose natural phytoplankton assemblages and larval …

Comparative Viability Of Spray Dried Lactobacilli Affected By Different Protective Agents And Storage Conditions, Vondel Vandeker Reyes Ortega

LSU Master's Theses

Because of their health benefits, probiotics are a significant part of the functional food industry. Spray drying is reported as the most common method used in the food industry to encapsulate probiotics. The objectives of this study were to investigate the effects of protective agents on the viability of lactobacilli after spray drying and during storage at different conditions and to evaluate spray drying conditions to produce these probiotic powders. Lactobacillus plantarum NRRL B-4496 (LP) and Lactobacillus acidophilus NRRL B-4495 (LA) were separately grown (~109 CFU/ml) and suspended in a 200 g/L solution of high maize starch (HM); maltodextrin (MD); …

Effects Of Timing Of Local Anesthesia On Physiological Responses In Calves After Dehorning, Amanda Jane Mathias

LSU Master's Theses

The American Veterinary Medical Association has stated the importance of minimizing pain associated with dehorning to limit the amount of distress and changes in the animal’s behavior and physiological states (AVMA, 2014b). The following studies aim to determine the effect of timing of lidocaine administration on physiological responses in calves after dehorning. The first study compared cortisol and substance P levels in calves dehorned immediately after administration of lidocaine and meloxicam, calves dehorned 10 minutes after the administration of lidocaine and meloxicam, and calves that were administered lidocaine and meloxicam and not dehorned. It was concluded waiting 10 minutes after …

Preferences And Values For The Gulf Coast Ocean Observing System, Cody Lynn Plummer

LSU Master's Theses

Integrated Ocean Observing Systems (IOOS) provide real time oceanic data and sea state forecasting information that is utilized by numerous public and private sectors engaging in maritime activities. The U.S. Gulf Coast constituent of this system (GCOOS) consists of 321 platforms, buoys, and sensors that provide measurements of wind speed, wave height, water quality, and other parameters. Government entities have proposed an expansion of this infrastructure by 40% at an estimated cost of $35 million for installation and $33 million annually for maintenance. As part of a larger project commissioned to estimate monetized benefits of this expansion, this study applied …

Role Of Varietal Resistance In Managing The Rice Water Weevil, A Major Insect Pest Of Louisiana Rice, Maisarah Mohamad Saad

LSU Master's Theses

Resistance of several commercial rice varieties widely grown in Louisiana was assessed against the rice water weevil (Lissorhoptrus oryzophilus Kuschel), the major insect pest of rice in the United States. A five-year field study was conducted to identify resistant genotypes among conventional inbred, herbicide tolerant, conventional hybrid, and herbicide tolerant hybrid varieties. Resistance was evaluated in two different locations to enable selection of rice varieties with wide adaptability over diverse environments. There were no large differences in the resistance found amongst commercial rice varieties over the five-year field study. Jefferson was frequently found to support lower larval densities than other …

Descriptive Aanalysis Of Agritourism In Louisiana: Motivation. Marketing. Limitations., Denys Maksymov

LSU Master's Theses

This study aims to present information on the existing Louisiana agritourism industry and create a descriptive profile of agritourism operators, addressing specifically what motivates people to engage in agritourism. A subsequent focus of the study is to document marketing approaches used in promoting agritourism operations and concerns and limitations faced by Louisiana agritourism operators. These will be achieved through the following objectives: 1. Use a survey instrument to collect information on the demographics of farmers interested and/or engaged in agritourism, farm characteristics, types of activities offered in the agritourism operations, as well as motivators for engaging in agritourism. 2. Analyze …

Design And Humoral Analysis Of Two Epitope-Based Brucella Abortus Dna Vaccines, Michael Mcgee

LSU Master's Theses

Brucella is a genus of Gram negative, facultative intracellular pathogens which cause brucellosis, one of the most wide spread zoonotic diseases. Brucellosis causes a severe economic burden due to reproductive loss in animals and human infection. Vaccination of animals has proven to be the most effective means of controlling brucellosis; however the current live-attenuated vaccines are not considered ideal. The live-attenuated vaccines Brucella abortus Strain 19 and RB51 remain pathogenic to humans, and the former interferes with diagnostic tests due to induction of antibodies against the O-polysaccharide. DNA vaccination with single antigens has proven to be successful at protecting mice …

Nealley's Sprangletop (Leptochloa Nealleyi Vasey) Management And Interference In Rice Production, Eric Allen Bergeron

LSU Master's Theses

A glasshouse study was conducted on the Louisiana State University campus in Baton Rouge to evaluate herbicide activity on Nealley's sprangletop. Herbicides were applied to Nealley’s sprangletop plants in the one-two tiller stage with height of 20-30 cm. Nealley's sprangletop control, leaf number, height, tiller number, and fresh weight biomass were evaluated. Nealley's sprangletop treated with glyphosate, quizalofop, fenoxaprop, and clethodim was controlled 89 to 99%. A field study was conducted at the LSU AgCenter Rice Research Station (RRS) and a grower location (GL) to evaluate herbicide rates and timings for control of Nealley's sprangletop in drill-seeded rice. Herbicide treatments …