Digital Commons Network^™

Effects Of Ambient Air Pollution On Asthma-Related Emergency Department Visits Within The Las Vegas Metropolitan Area, Loiren E. Monardes

UNLV Theses, Dissertations, Professional Papers, and Capstones

The objective of this research was to evaluate the risk for asthma-related Emergency Department visits and their association with ambient air pollution within the Las Vegas metropolitan area. All data were aggregated by date and ZIP Code. The association was analyzed by applying the distributed lag non-linear model in an attempt to identify elevated concentrations of specific air pollutants

as triggers and their delayed effects (lag days). Relative Risk (RR) and 95% confidence intervals were produced, while adjusting for socioeconomic status. This ecological population-based study analyzed daily asthma counts of Emergency Department visits from January 1st, 2009 to December 31st, …

Comparison Of Cervical Angle During Different Equipment Removal Techniques Used In Ice Hockey For Suspected Cervical Injuries, Kendell Sarah Galor

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of the study was to compare cervical movement between two different methods of removal of the helmet and shoulder pads. Seven college age male subjects (height: 172.54 ± 7.98 cm; weight: 85.70 ± 15.75 kg) with hockey experience volunteered to participate in the study. Each subject agreed to and signed the institutional review board approved informed consent before articipation. Subjects used their own game fit hockey helmet and were fitted with a pair of shoulder pads (CCM Tacks 1052 Sr Shoulder Pads). Cervical spine motion was measured using 3D kinematics (Vicon Motion Systems Ltd., UK). Reflective markers affixed …

Examining Environmental Hazards In Rental Homes And Habitability Laws In Clark County, Nevada, Jorge Luis Bertran

UNLV Theses, Dissertations, Professional Papers, and Capstones

It is well established that home conditions are linked to the health outcomes of occupants. There are over 880,000 housing units in Clark County, Nevada; nearly half of those are renter-occupied units (ROUs). Currently, there is limited research on the characteristics of environmental hazards found in Clark County ROUs and the strength of habitability statutes created to protect tenants from substandard housing. Understanding how renters in Clark County are affected by environmental hazards in ROUs and the processes by which landlords and tenants resolve grievances related to those hazards would benefit public health. It would enhance the ability to quickly …

Prokaryotic Diversity And Aqueous Geochemistry Of Subsurface Environments Of The Death Valley Regional Flow System, Joshua David Sackett

UNLV Theses, Dissertations, Professional Papers, and Capstones

This dissertation summarizes over four years of effort towards the completion of a Ph.D. in Biological Sciences. The work presented in this document covers a broad range of topics, but a central unifying theme is prokaryotic life in the continental subsurface. The work presented in each chapter relied heavily on cultivation-independent methods for assessing prokaryotic communities, including prokaryotic community structure reconstruction from high-throughput sequencing of 16S rRNA gene libraries and single-cell genome analysis of novel uncultivated bacteria.

Chapter 2 examines the aqueous geochemistry and prokaryotic diversity of Devils Hole, a cavernous limnocrene and sole natural habitat for the critically endangered …

Cocoa, Postprandial Lipoproteins, And Inflammation In Type 2 Diabetes: A Secondary Data Analysis, Rickelle Tallent

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: Type 2 Diabetes (T2D) is associated with dyslipidemia and chronic low-grade inflammation, which greatly increases the risk of cardiovascular disease (CVD) in this population. Hyperlipidemia and inflammation in the postprandial state substantially contribute to atherosclerosis development and CVD risk, and have shown to be exacerbated by high-fat meals. Dietary cocoa is a rich source of flavanols that is associated with favorable effects on cardiovascular health, such as improvements in the lipid profile and chronic inflammation, in non-diabetic adults.

Objective: To conduct a secondary data analysis using unpublished data from a previously conducted clinical study to determine whether acute cocoa …

Mob-Ndr Kinase Signaling Components Are Required For Epithelial Tube Formation In The Drosophila Follicular Epithelium, Juan Carlos Duhart

UNLV Theses, Dissertations, Professional Papers, and Capstones

A major goal of developmental biology is to understand how a single fertilized cell can give rise to the many functional tissues and organs, of specific sizes and shapes, that make up the adult body plan. Over the last 25 years, developmental geneticists have uncovered much concerning the cell-to-cell communication systems that are necessary to build complex tissues and organs. For example, throughout development, cells communicate with their neighbors using specialized signaling molecules. These signals are instructive and provide “signal-receiving” cells with information about space and time. That is, signal-receiving cells “learn” precisely where they are located, and, how far …

Expression Of Line-1 In Human Somatic Tissues And The Factors Correlated With Line- 1 Expression, G. M. Jonaid

UNLV Theses, Dissertations, Professional Papers, and Capstones

Despite the long-held assumption that transposons are normally only expressed in the germline, recently we discovered that full length or partial transcripts of LINE1 are frequently found in the somatic cells. However, the extent of variation in LINE1 levels across different tissues and different individuals, and the genes and pathways that are co-expressed with LINE1 are unknown. Co-expressed genes may be candidate genes that are functioning in transposon silencing. Here, we report the extent of variation in L1HS expression levels across cancer tissues and healthy tissues collected for The Cancer Genome Atlas (TCGA). L1HS is overexpressed in most of the …

Handgrip Fatigue And Forearm Girth In Intermediate Sport Rock Climbers, Grace Antoinette Macdonald

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: Rock climbing has been increasing in popularity both recreationally and competitively. Indoor sport rock climbing is a type of climbing where the climber ascends a wall using artificial rocks (hand and foot holds) and is attached to a safety rope. Despite this increase in popularity of the sport, the physiological responses to sport climbing as an exercise to specific muscle groups are not well defined in literature. Purpose: The purpose of this study was to quantify the change in handgrip strength over a 30-minute bout of continuous climbing, specifically in intermediate sport climbers. An additional aim of this study …

An Evaluation Of Alzheimer's Disease-Related Pathology In Two Different Models Of Diabetes In Immune-Challenged Mice, Andrew Scott Murtishaw

UNLV Theses, Dissertations, Professional Papers, and Capstones

Obesity, type 2 diabetes mellitus (T2DM), and metabolic syndrome are related disorders with wide-ranging and devastating effects that can be observed throughout the body. One important and understudied organ of damage is the brain. Clinical and epidemiological studies have found that T2DM, and more specifically hyperinsulinemia, significantly increases the risk of cognitive decline and increases the likelihood of Alzheimer’s disease (AD) and other forms of dementia in the elderly. Insulin has slightly different functions in the peripheral body than in the central nervous system and the dysregulation of these functions may contribute to the onset and progression of late-life neurodegenerative …

Biomechanical Analysis Of Jumping: The Influence Of External Load And Countermovement Depth On Deceleration Strategies And Performance, Leland Adam Barker

UNLV Theses, Dissertations, Professional Papers, and Capstones

Jumping performance has traditionally been measured by jump height alone. In recent years, the reactive strength index (RSI = Jump height / jump time)) has been used as another measure of jump performance. According to RSI, which was developed to assess eccentric force production, jump performance can improve by increasing jump height, decreasing jump time, or both simultaneously. However, it is not clear how force production correlates to RSI variables. If RSI is meant to be a practical measure of eccentric force production, it should correlate strongly to eccentric and amortization force production during jumping. Thus, the purpose of the …

Prevalence Of Cariogenic Microbial Flora Among Scardovia Wiggsiae-Positive And Negative Patients, Amy Tam

UNLV Theses, Dissertations, Professional Papers, and Capstones

The formation of dental caries (cavities) is a complex, multi-dimensional process that necessarily involves many risk factors – including the acquisition and colonization of cariogenic oral bacteria. The most frequently associated oral pathogens are the acid-producing and acid-tolerant oral streptococcus species, such as Streptococcus mutans (S. mutans or SM). Many studies have established and confirmed the critical role of the formation of biofilm in the virulence of S. mutans, and the critical role this may play in determining the balance of the oral microbiome towards health or disease.

More recent efforts have discovered a novel cariogenic pathogen, Scardovia wiggsiae (S. …

Larval-Ant Interactions In The Mojave Desert: Communication Brings Us Together, Alicia Mellor

UNLV Theses, Dissertations, Professional Papers, and Capstones

Butterflies are a diverse and essential group of pollinators whose abundance is predominantly determined by growth and survival of their larvae. In the family Lycaenidae, many species participate in a larval-ant mutualism where ants feed on nutrient-rich nectar produced by larvae and, in turn, protect those larvae from predators. Emerging evidence indicates larval scent in the form of cuticular hydrocarbons (CHCs), not nectar production, drives this interaction. This study takes two approaches to investigate CHCs as the driver behind the larval-ant mutualism in three butterfly species native to southern Nevada: Euphilotes bernardino martini (Martin’s blue), Brephidium exilis (western pygmy blue), …

Effect Of Exercise And Hypoxia On Plasma Telomerase, Charli D. Aguilar

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Telomerase reverse transcriptase (TERT) is the enzyme that adds telomeric sequences to the end of linear chromosomes. Exercise has shown to upregulate acutely leukocyte TERT after just 30 minutes of running on a treadmill at 80% of VO2max (Denham et al., 2016). Hypoxia inducible factor 1 (HIF-1) is also a mediator of TERT in in vitro (Nishi et al., 2004). Moderate acute exposure to hypoxia was associated with substantial increases in plasma TERT in a recent study on rats (Wang et al., 2014). The specific aim of the current study was to identify if acute hypoxia upregulates plasma TERT …

Money Matters: Exploring The Financial Resources For Sports Medicine Programs In Public And Private Secondary Schools--A Qualitative Study, Natalie Mercedes Aviles Flores

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Institutional funding and strained school budgets are a barrier to placing certified athletic trainers (ATCs) in secondary schools. The size and location of a school appear to be coinciding limitations (Mazerolle et al, 2015), but what is lacking in the literature is an

evaluation of how schools manage to provide adequate medical coverage. Resources are available, but there is question as to whether these schools consider or even utilize them.

Objective: The aim of this qualitative study is to explore the funding techniques and budget sources of high school athletic training clinics from the perspective of both private and …

Movement Efficiency Through Autonomy Support, Takehiro Iwatsuki

UNLV Theses, Dissertations, Professional Papers, and Capstones

Supporting performer autonomy has been consistently been shown to enhance motor learning (for reviews, see Sanli, Patterson, Bray, & Lee, 2013; Wulf, 2007; Wulf & Lewthwaite, 2016). Autonomy-supportive situations are those in which learners are given control over aspects of the practice conditions or are provided with other choices, including small and incidental choices that are not necessarily related to the task at hand. Providing autonomy support also benefits immediate motor performance, as demonstrated by enhanced punching velocity and impact forces in a study involving skilled kick boxers (Halperin, Chapman, Martin, Lewthwaite, & Wulf, 2016). Autonomy support is a key …

Transcriptional Silencing And Anti-Silencing Of Virulence Genes In The Bacterial Pathogen Shigella Flexneri: Virb, Dna Supercoiling, And The Histone-Like Nucleoid Structuring Protein, Michael Adam Picker

UNLV Theses, Dissertations, Professional Papers, and Capstones

Transcriptional silencing and anti-silencing affect many aspects of bacterial physiology, including virulence in bacterial pathogens. In Shigella species, a group of gram-negative pathogens that cause bacillary dysentery in humans, the histone-like nucleoid structuring protein (H-NS) transcriptionally silences virulence genes found on the large virulence plasmid while VirB anti-silences these genes. However, the mechanistic details of their interplay are not fully understood. To elucidate their regulatory mechanisms, I use the icsP virulence locus, which shares a long intergenic region with the divergently transcribed ospZ gene (1535 bp from TSS to TSS). Prior to this work, two discrete H-NS binding regions had …

The Acute Effects Of Cupping Therapy On Hamstring Range Of Motion Compared To Sham, Matthew Schafer

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Flexibility is an important aspect of physical performance and when deficient can result in an increased opportunity for injury. Cupping therapy is an ancient technique that has recently seen a growth in popularity in Western Orthopedic medicine as a soft tissue mobilization technique. Most cupping therapy research explores the use of cupping therapy for treating headache, herpes zoster, asthma, cough, and other non-orthopedic pathologies. Cupping therapy has had positive results on an injured population for increasing flexibility. Objective: To identify if cupping therapy applied passively for 10 minutes results in an increase in flexibility, and to identify if there …

Screening For The Novel Cariogenic Pathogen Scardovia Wiggsiae Among Orthodontic Patients, Adam Whiteley

UNLV Theses, Dissertations, Professional Papers, and Capstones

Screening for the Novel Cariogenic Pathogen Scardovia wiggsiae among Orthodontic Patients

by

Adam Whiteley

Dr. Karl Kingsley, Examination Committee Chair Professor of Biomedical Sciences University of Nevada, Las Vegas School of Dental Medicine

The recent discovery of a novel cariogenic pathogen Scardovia wiggsiae has led many scientists and oral health researchers to re-evaluate and re-examine existing saliva repositories to determine the prevalence among patient populations. Recent efforts at this institution have used existing saliva samples to determine the prevalence among both adults and pediatric patients. These studies have revealed this organism may be found in approximately one-quarter of all samples …

Effect Of Bmp Treatment On Periostin Gene Expression In Pre-Osteoblastic Mc3t3-E1 Mouse Cells, Vincent Lee Khang

UNLV Theses, Dissertations, Professional Papers, and Capstones

Periostin is a secreted, extracellular matrix (ECM) protein widely expressed within collagen-rich fibrous connective tissues of the body including the periodontal ligament (PDL), bone, skin, heart, and cornea. Periostin has been shown to serve many important regulatory functions including cell adhesion, cell motility, wound healing and of particular importance to the dental field, differentiation of osteoblasts. The deletion of periostin compromises osteoblast attachment to bone matrix and induces a reduction in mineralization and expression of bone markers, including type I collagen, osteocalcin, osteopontin and alkaline phosphatase. Periostin has also been shown to play a significant role in collagen fibrillogenesis by …

Changes In Igf-1 Levels Post Deer Antler Velvet Supplementation, Nicole Alexandra Marmillo

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Deer antler velvet has been used in traditional Chinese medicine for thousands of years but has recently gained popularity in Western medicine as an ergogenic aid. According to Oriental medicine deer antler velvet can be attributed to enhancing immune system function, improving athletic performance, increasing muscle recovery, enhancing sexual function, improving disease recovery, and enhancing cardiovascular function. Deer antler velvet is orally taken in pill, powder or spray form. Although a number of health and human performance effects have been attributed to deer antler velvet the scientific rationale behind these beliefs is ambiguous Objective: The purpose of this study …

Digging Biomechanics In Geomyoid Rodents, Alexis Moore Crisp

UNLV Theses, Dissertations, Professional Papers, and Capstones

Subterranean digging behaviors provide opportunities for protection, access to prey, and predator avoidance for a diverse array of vertebrates, yet studies of the biomechanics of burrowing have been limited by the technical challenges of measuring kinetics and kinematics of animals moving within a substrate. Prior studies of burrowing have recorded a single axis of x-ray video and/or force. However, empirical observations show that burrowing is not restricted to a single axis or plane. I describe a new system called a ‘tunnel-tube’ for measuring 3D reaction force during burrowing. This tunnel-tube has two separate tubes, one ‘entry tube’ that has no …

An Examination Of The Community And Environmental Impact Of A Las Vegas Convention Food Rescue Program, Samantha H. To

UNLV Theses, Dissertations, Professional Papers, and Capstones

Food insecurity affects millions of Americans each year, inflicting negative consequences on the physical and mental health outcomes at all ages. Concurrently, millions of tons of leftover edible foods are sent to landfill each year. Not only does this pose a question of ethics, but increasingly harms the environment by misusing resources and increasing the release of

greenhouse gases.

Food rescue advisory meetings highlighted the need for more focused research to be conducted that assessed the impacts of donating rescued convention food on local environmental and community health. A mixed-method approach was used to detail the food rescue process between …

The Caspase Cascade During Hibernation In The Golden-Mantled Ground Squirrel, Spermophilus Lateralis, Michael David Treat

UNLV Theses, Dissertations, Professional Papers, and Capstones

In several human pathologies like heart attack, stroke, neurodegenerative diseases, and autoimmune disorders, widespread cell death, or apoptosis, is a major cause of organ dysfunction and death. Hibernating golden-mantled ground squirrels, Spermophilus lateralis, experience numerous conditions during the winter that are known to be pro-apoptotic in other mammal systems (e.g. extreme hypothermia, ischemia and reperfusion, acidosis, increased reactive oxygen species, bone and muscle disuse). However, studies suggest that hibernators may invoke a protective phenotype to limit widespread cell damage and loss during the hibernation season. Could regulating apoptosis provide protection against the harmful conditions experienced during the hibernation season? Could …

Does Distance Climbed In A 30-S Maximal Effort Test On A Simulated Climbing Machine Correlate With Wingate Variables?, Kayla Wright

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: The Wingate Anaerobic Test (WAnT) is used to assess anaerobic capacity by measuring total work. In addition to total work, the WAnT measures peak power, mean power, relative peak power, and fatigue. There are lower-, upper-, and full-body alternatives to the WAnT that can be used to measure anaerobic capacity, but there is little data on these alternatives. It may be beneficial to have a full-body assessment of anaerobic capacity, as many sporting events have full-body anaerobic demands. Simulated climbing machines are becoming popular modes of exercise. They have been compared to treadmill running and cycling in terms of …