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Aboveground Herbivory Influences Belowground Defense Responses In Maize, Lise Pingault, Saumik Basu, Prince Zogli, W. Paul Williams, Nathan Palmer, Gautam Sarath, Joe Louis

Department of Entomology: Faculty Publications

The European corn borer (ECB; Ostrinia nubilalis) is an economically damaging insect pest of maize (Zea mays L.), an important cereal crop widely grown globally. Among inbred lines, the maize genotype Mp708 has shown resistance to diverse herbivorous insects, although several aspects of the defense mechanisms of Mp708 plants are yet to be explored. Here, the changes in root physiology arising from short-term feeding by ECB on the shoot tissues of Mp708 plants was evaluated directly using transcriptomics, and indirectly by monitoring changes in growth of western corn rootworm (WCR; Diabrotica virgifera virgifera) larvae. Mp708 defense responses …

Effect Of Cry1f Maize On The Behavior Of Susceptible And Resistant Spodoptera Frugiperda And Ostrinia Nubilalis, Ana María Vélez, Analiza P. Alves, Erin E. Blankenship, Blair Siegfried

Department of Entomology: Faculty Publications

Understanding the behavior of pests targeted with Bacillus thuringiensis Berliner (Bt) crops is important to define resistance management strategies. Particularly the study of larval movement between plants is important to determine the feasibility of refuge configurations. Exposure to Bt maize, Zea mays L. (Poaceae), has been suggested to increase larval movement in lepidopteran species but few studies have examined the potential for resistance to interact with behavioral responses to Bt toxins. Choice and no-choice experiments were conducted with Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) and Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) to determine whether Cry1F resistance influences neonate movement. Leaf discs …

Choosing A Bt Transgenic Corn Hybrid, R. J. Wright, Thomas E. Hunt, J. F. Witkowski, Blair Siegfried, John E. Foster

John E. Foster

Describes traits of various Bt corn hybrids and factors to consider when selecting and using these hybrids.

Transcriptional Analysis Of Susceptible And Resistant European Corn Borer Strains And Their Response To Cry1f Protoxin, Neetha Nanoth Vellichirammal, Haichuan Wang, Seong-Il Eyun, Estuko N. Moriyama, Brad Coates, Nicholas Miller, Blair Siegfried

Department of Entomology: Faculty Publications

Background: Despite a number of recent reports of insect resistance to transgenic crops expressing insecticidal toxins from Bacillus thuringiensis (Bt), little is known about the mechanism of resistance to these toxins. The purpose of this study is to identify genes associated with the mechanism of Cry1F toxin resistance in European corn borer (Ostrinia nubilalis Hübner). For this, we compared the global transcriptomic response of laboratory selected resistant and susceptible O. nubilalis strain to Cry1F toxin. We further identified constitutive transcriptional differences between the two strains.

Results: An O. nubilalis midgut transcriptome of 36,125 transcripts was assembled de novo from …

Estimating The Frequency Of Cry1f Resistance In Field Populations Of The European Corn Borer (Lepidoptera: Crambidae), Blair D. Siegfried, Murugesan Rangasamy, Haichuan Wang, Terence A. Spencer, Chirakkal V. Haridas, Brigitte Tenhumberg, Douglas V. Sumerford, Nicholas P. Storer

School of Biological Sciences: Faculty Publications

Background: Transgenic corn hybrids that express toxins from Bacillus thuringiensis (Bt) have suppressed European corn borer populations and reduced the pest status of this insect throughout much of the US corn belt. A major assumption of the high-dose/refuge strategy proposed for insect resistance management and Bt corn is that the frequency of resistance alleles is low so that resistant pests surviving exposure to Bt corn will be rare. Results: The frequency of resistance to the Cry1F Bt toxin was estimated using two different screening tools and compared with annual susceptibility monitoring based on diagnostic bioassays and LC_{50 …}

Use Of Spectral Vegetation Indices Derived From Airborne Hyperspectral Imagery For Detection Of European Corn Borer Infestation In Iowa Corn Plots, Matthew W. Carroll, John A. Glaser, Richard L. Hellmich, Thomas E. Hunt, Thomas W. Sappington, Dennis Calvin, Ken Copenhaver, Jon Fridgen

Department of Entomology: Faculty Publications

Eleven spectral vegetation indices that emphasize foliar plant pigments were calculated using airborne hyperspectral imagery and evaluated in 2004 and 2005 for their ability to detect experimental plots of corn manually inoculated with Ostrinia nubilalis (Hübner) neonate larvae. Manual inoculations were timed to simulate infestation of corn, Zea mays L., by first and second fiights of adult O. nubilalis. The ability of spectral vegetation indices to detect O. nubilalis-inoculated plots improved as the growing season progressed, with multiple spectral vegetation indices able to identify infested plots in late August and early September. Our findings also indicate that for …

Effect Of Age And Mating Status On Adult European Corn Borer (Lepidoptera: Crambidae) Dispersal From Small-Grain Aggregation Plots, Brendon J. Reardon, Thomas W. Sappington

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), is often controlled with genetically modified corn, Zea mays L., hybrids (Bacillus thuringiensis [Bt] corn) in the United States. If Bt-resistant insects are detected in the field, mitigation-remediation tactics must be implemented to sustain the efficacy of insecticidal, transgenic corn. Mass releasing laboratory reared, susceptible adults near aggregation sites to mate with locally emerging resistant adults is a possible remediation tactic, but it is imperative that the former remain in or near the release site long enough to mate. Understanding adult dispersal behavior relative to the timing of mating is …

G07-1782 First Generation European Corn Borer Scouting And Treatment Decisions, R. J. Wright, Robert J. Wright

University of Nebraska-Lincoln Extension: Historical Materials

The decision to treat for European corn borer is complex and affected by many variables such as weather, plant maturity, borer survival and development, anticipated corn prices, insecticide efficacy, and costs versus anticipated returns. This 2007 NebGuide discusses the factors growers need to consider when assessing the need for control of first generation European corn borers in non-Bt corn.

G07-1783 Second Generation European Corn Borer Scouting And Treatment Decisions, Robert J. Wright

University of Nebraska-Lincoln Extension: Historical Materials

The decision to treat for European corn borer is complex and affected by many variables, such as weather, plant maturity, borer survival and development, anticipated corn prices, insecticide efficacy, and costs versus anticipated returns. This 2007 NebGuide discusses the factors growers need to consider when assessing the need for control of second generation European corn borers in non-Bt corn.

Dispersal Of Newly Eclosed European Corn Borer Adults (Lepidoptera: Crambidae) From Corn Into Small-Grain Aggregation Plots, Brendon J. Reardon, Douglas V. Sumerford, Thomas W. Sappington

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Genetically modified, insecticidal Bacillus thuringiensis (Bt) corn, Zea mays L., hybrids are used throughout the Corn Belt for European corn borer, Ostrinia nubilalis(Hübner) (Lepidoptera: Crambidae), control. To slow development of Bt corn resistance, the Environmental Protection Agency requires growers to plant a refuge. Determining the appropriate distance between a refuge and Bt corn, and development of mitigation-remediation strategies such as mass releases of susceptible moths, requires an understanding of adult dispersal and mating behavior. However, much remains unknown about these behaviors. Because mating often occurs in grass near cornfields where adult O. nubilalis aggregate, we planted small-grain plots as …

Adult Dispersal Of Ostrinia Nubilalis Hübner (Lepidoptera: Crambidae) And Its Implications For Resistance Management In Bt-Maize, J. A. Qureshi, L. L. Buschman, James E. Throne, S. B. Ramaswamy

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dispersal of European corn borer, Ostrinia nubilalis Hubner was examined by release and recapture of the dye marked adults and by capture of the feral adults in and around the large 50 ha center pivot irrigated fields of Bacillus thuringiensis (Bt) maize. Pheromone and black light traps were used to catch the adults. In 1999, 15 094 marked males and 7993 marked females were released, and in 2001, 13 942 marked males and 9977 marked females were released. In 1999, maximum mean recapture beyond the release point was 1.95 and 1.67% for males and females, but in 2001, the recapture …

Ec05-1572 Corn Insects I, Robert J. Wright, Terry A. Devries, James A. Kalisch

University of Nebraska-Lincoln Extension: Historical Materials

This two-page, 4-color extension circular covers the identification and management of Nebraska corn insects. These include: European corn borer, Western bean cutworm, corn earworm, armyworm, fall armyworm, common stalk borer, and black cutworm.

Cross-Resistance Of Cry1ab-Selected Ostrinia Nubilalis (Lepidoptera: Crambidae) To Bacillus Thuringiensis Δ -Endotoxins, Herbert A.A. Siqueria, Daniel J. Moellenbeck, Terrence A. Spencer, Blair D. Siegfried

Department of Entomology: Faculty Publications

Corn plants expressing the toxin from Bacillus thuringiensis (Berliner) have proven to be effective in controlling lepidopteran pests such as the European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae). Several Bt toxins are being tested and incorporated into crop genomes, although tests for cross-resistance among different toxins have been limited by a lack of resistant colonies. Four different colonies of O. nubilalis selected with full-length Cry1Ab incorporated into artificial diet developed significant levels of resistance (2.0- to 10-fold) within 10 generations. Additionally, selection with Cry1Ab resulted in decreased susceptibility to a number of other toxins to which the selected colonies …

In Vivo And In Vitro Metabolism Of Fi Pronil By Larvae Of The European Corn Borer Ostrinia Nubilalis, Eric W. Durham, Blair D. Siegfried, Michael E. Scharf

Department of Entomology: Faculty Publications

In vivo and in vitro metabolism of [₁₄C]fi pronil was examined in a susceptible European corn borer (Ostrinia nubilalis, Hübner) laboratory strain. [₁₄C]Fipronil penetrated the larval integument slowly, with 71.5% of the applied radioactivity recovered from surface rinses 24 h after topical application. Despite this slow penetration, radioactivity was detected in both the excrement and internal organo-soluble fractions. Radioactivity in the internal aqueous fraction and tissue pellet accounted for less than 0.8% of total radioactivity. The in vivo studies suggest that fipronil oxidation to its sulfone metabolite is the major route of metabolic conversion. …

Geostatistical Analysis Of The Small-Scale Distribution Of European Corn Borer (Lepidoptera: Crambidae) Larvae And Damage In Whorl Stage Corn, Robert J. Wright, Terry A. Devries, Linda J. Young, Keith J. Jarvi, Ronald C. Seymour

Department of Entomology: Faculty Publications

The small-scale spatial distribution of European corn borer, Ostrinia nubilalis (Hübner), larvae and damage in whorl stage corn, Zea mays L., was characterized using geostatistics. Spatial distribution of O. nubilalis larval feeding damage was studied at Clay Center, North Platte, and Concord, NE, during June-July 1992-1994, and spatial distribution of O. nubilalis larvae and damage was studied at Clay Center in June 1997. Semivariograms were calculated to model the change in spatial correlation with increasing distance between samples. Spatial distribution of larval damage during 1992-1994 was best described using a spherical model. Damage was spatially correlated among plants at distances …

Development Of European Corn Borer Larvae On Event 176 Bt Corn: Influence On Survival And Fitness, Blair D. Siegfried, Amelia C. Zoerb, Terrence A. Spencer

Department of Entomology: Faculty Publications

European corn borer larvae, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) that have completed development on Event 176 Bt corn hybrids have survived exposure to sublethal doses of the Cry1Ab Bt toxin or are exploiting plant tissues that do not express the toxin. To evaluate the impact of such exposure, diapausing larvae were collected from Event 176 and conventional hybrids and compared for rates of pupation, parasitism, fitness (pupal weight, longevity, and fecundity) and susceptibility to the Cry1Ab toxin. Larvae completing development on Event 176 corn exhibited approximately 10% higher survival rates and correspondingly lower parasitism rates than larvae completing development on …

Development Of Diagnostic Concentrations For Monitoring Bacillus Thuringiensis Resistance In European Corn Borer (Lepidoptera: Crambidae), Paula C.R.G. Marcon, Blair D. Siegfried, Terrence A. Spencer, W. D. Hutchinson

Department of Entomology: Faculty Publications

Two candidate diagnostic concentrations of the Cry1Ab and Cry1Ac toxins from Bacillus thuringiensis corresponding to the LC₉₉ and EC₉₉ (effective concentration that causes 99% growth inhibition) for European corn borer, Ostrinia nubilalis (Hubner), were determined based on previously obtained baseline data. Validation experiments using field-collected European corn borer populations from across North America showed that for Cry1Ab, a concentration corresponding to the upper limit of the 95% confidence interval of the LC₉₉, produced mortality .99% for all populations tested. However, for Cry1Ac, adjustments and further validation are probably necessary. Development of B. thuringiensis resistance monitoring programs …

Comparison Of Bt (Bacillus Thuringiensis Berliner) Maize And Conventional Measures For Control Of The European Corn Borer (Lepidoptera: Crambidae), Thomas L. Clark, J. E. Foster, Shripat T. Kamble, Elvis A. Heinrichs

Department of Entomology: Faculty Publications

Field experiments were conducted in 1997 to compare the efficacy Bt (Bacillus thuringiensis Berliner) maize hybrids and two conventional measures for control of the European corn borer, Ostrinia nubilalis (Hubner). Treatments consisted of transgenic Bt-maize hybrids and their non-Bt isolines, and isolines treated with a formulated Bt or permethrin insecticide. All control measures significantly reduced O. nubilalis damage in terms of tunnels per plant, length of tunneling, and larvae per plant. The following hierarchy in terms of O. nubilalis efficacy was observed: transgenic Bt > permethrin > formulated Bt > control. In most cases, transgenic Bt maize was most effective in preventing …

Choosing A Bt Transgenic Corn Hybrid, R. J. Wright, Thomas E. Hunt, J. F. Witkowski, Blair Siegfried, John E. Foster

University of Nebraska-Lincoln Extension: Historical Materials

Describes traits of various Bt corn hybrids and factors to consider when selecting and using these hybrids.

Random Amplified Polymorphic Dna Used To Identify Genetic Variation In Ecotypes Of The European Corn Borer (Lepidoptera: Pyralidae), Saowaluck Pornkulwat, Steven R. Skoda, Gustave D. Thomas, John E. Foster

Department of Entomology: Faculty Publications

The European corn borer, Ostrinia nubilalis (Hubner), has 3 morphologically indistinguishable voltinism ecotypes. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to discriminate ecotypes of the European corn borer. Genomic DNA samples from the European corn borer were screened with a total of 120 random primers. Ten of these primers yielded 21 clear and reproducible RAPD markers after agarose gel electrophoresis. Dendrograms constructed using the Nei and Li distance matrix of the phylogenetic relationships, among and within ecotypes, of the European corn borer showed the multivoltine ecotype to be genetically separated from univoltine and bivoltine ecotypes. The dendrogram of …

Nf98-365 Second Generation European Corn Borer Scouting And Treatment Decisions, Robert J. Wright, John F. Witkowski

University of Nebraska-Lincoln Extension: Historical Materials

This NebFact discusses the factors growers need to consider when assessing the need for control of second generation European corn borers in non-Bt corn.

G96-1300 Insects That Feed On Corn Ears, David L. Keith, J. F. Witkowski

University of Nebraska-Lincoln Extension: Historical Materials

How to identify mature and immature insects that infest and damage ears of corn.

Several kinds of insects infest the developing ears of corn, raising farmers' concerns and sometimes causing economic damage. Some of these insects can reduce both yield and quality of seed corn, pop corn, sweet corn and field corn. Control decisions depend in part on the economics of the corn crop produced. In many cases, particularly in field corn where the value of the crop per acre is somewhat lower, the insects may not be sufficiently damaging to require control. The higher value of seed corn compared …

First Generation European Corn Borer Management, Murdick J. Mcleod

SDSU Extension Extra Archives

The European corn borer is a destructive pest of corn in the Midwest. Larvae feed on leaves and tunnel into stalks and ear shanks. Stalk tunneling often interferes with nutrient transport and predisposes these plants to lodging. Additionally, yields may be impacted form damage to corn shanks thus leading to late-season ear drop. Although the corn borer damages corn each year, it is largely ignored by many South Dakota growers.

Second Generation European Corn Borer Management, Murdick J. Mcleod

SDSU Extension Extra Archives

The European corn borer is a destructive pest that reduces corn yields each year in the Midwest. Larvae feed on leaves and tunnel into stalks and ear shanks. Stalk tunneling often interferes with nutrient transport and predisposes these plants to lodging. Additionally, yields may be impacted from damage to corn shanks thus leading to late-season ear drop.

Ec91-1554 European Corn Borer Larval Size Card

University of Nebraska-Lincoln Extension: Historical Materials

This extension circular shows the following statistics of the European Corn Borer Ostrinia nubilalis (Hubner): larval instar, body length range, prothoracic shield width, and head capsule width. The larva shown is larger than life size.

Ec86-1541 Corn Insects — Above Ground

University of Nebraska-Lincoln Extension: Historical Materials

This 4-color extension circular was prepared by Extension entomologists of the North Central States in cooperation with the Federal Extension Service, U.S. Department of Agriculture. It shows the following corn insects:

1. European corn borer (early leaf feeding and mature borers)

2. Southwestern corn borer

3. Common stalk borer

4. Chinch bug

5. Corn earworm

6. Armyworm

7. Corn rootworm beetles (lefet to right: Northern, Western, and Southern)

8. Grasshopper

9. Corn leaf aphid

10. Corn flea beetle and damage

Monitoring And Control Strategies For The European Corn Borer, Ostrinia Nubilalis (Hubner), In Massachusetts Sweet Corn., Gordon Howard Fletcher-Howell

Masters Theses 1911 - February 2014

No abstract provided.

European Corn Borer Parasitoids; Distribution In Southern Minnesota, W. V. Winnie, D. D. Sreenivasam, H. C. Chiang

Journal of the Minnesota Academy of Science

During the 1940's and 1950's seven exotic parasitoids of the European corn borer (ECB) Ostrinia nubilalis (Hubner), were released in Minnesota. This study was done to determine which introduced parasitoids became established and their distribution in Minnesota. The experiment was conducted during the summer of 1977 and fall of 1977, 78, 79- and 80 in conjunction with the annual ECB population surveys in southern Minnesota by the State Department of Agriculture, Division of Plant Industry. Three introduced parasitoids, Macrocentrus grandii Goidanich, Eriborus Terebrans (Gravenhorst), and Sympiesis viridula (Thomson) and two native parasitoids, lshnus inquisitorius artricollaris (Walsh) and Aplomya caesar (Aldrich) …

Seasonal Abundance Of The European Corn Borer Ostrinia Nubilalis (Hbn.) Within The North Central United States, R. E. Hill, H. C. Chiang, A. J. Keaster, W. B. Showers, G. L. Reed

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Annual changes in corn borer populations in the North Central States were measured as a phase of a Regional Project, NC-20, entitled "Factors Influencing Corn Borer Populations" in Minnesota, Iowa, Kansas, Nebraska, Missouri and Ohio over a period of years. Standardized procedures were followed to estimate the seasonal abundance of the European corn borer, Ostrinia nubilalis (Hbn.), under cropping procedures in different locations within the North Central States. The primary purpose of the present bulletin is to present results for the 1965 to 1969 period and to compare these results with the previous 10 years and thus attempt to analyze …

European Corn Borer, Ostrinia Nubilalis (Hbn.) Populations In Field Corn, Zea Mays (L.) In The North Central United States, R. E. Hill, A. N. Sparks, C. C. Burkhardt, H. C. Chiang, M. L. Fairchild, W. D. Guthrie

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

A long-range study of the annual changes in corn borer populations in the North Central States was started in Minnesota, Iowa, Kansas and Nebraska in 1955 and in Missouri and Ohio in 1956. This investigation was a phase of a broader Regional Project, NC-20, entitled "Factors Influencing Corn Borer Populations" and was undertaken to measure by standardized procedures the seasonal changes in abundance of the European corn borer, Ostrinia nubilalis (Hbn.), under cropping procedures in different locations within, the North Central States. Much valuable information has been accumulated on the abundance and effects of various physical and biotic factors on …