Digital Commons Network^™

Effects Of The Antimicrobial Tylosin On The Microbial Community Structure Of An Anaerobic Sequencing Batch Reactor, Toshio Shimada, Xu Li, Julie L. Zilles, Eberhard Morgenroth, Lutgarde Raskin

Department of Civil and Environmental Engineering: Faculty Publications

The effects of the antimicrobial tylosin on a methanogenic microbial community were studied in a glucosefed laboratory-scale anaerobic sequencing batch reactor (ASBR) exposed to stepwise increases of tylosin (0, 1.67, and 167 mg/L). The microbial community structure was determined using quantitative fluorescence in situ hybridization (FISH) and phylogenetic analyses of bacterial 16S ribosomal RNA (rRNA) gene clone libraries of biomass samples. During the periods without tylosin addition and with an influent tylosin concentration of 1.67 mg/L, 16S rRNA gene sequences related to Syntrophobacter were detected and the relative abundance of Methanosaeta species was high. During the highest tylosin dose of …

An Evaluation Of Novel Approaches To Antimicrobial Control Of Oral Biofilms, Manisha Jindal

Digitized Theses

An Evaluation of Novel Approaches to Antimicrobial Control of Oral Biofilms

Introduction: Streptococcus mutans (SM) and Haemophilus actinomycetemcomitans (HA) are important pathogens in the development of caries and periodontitis. This thesis investigated several novel strategies aimed at preventing their growth and biofilm forming capacity.

Methods: SM and HA biofilms were challenged with numerous antimicrobial solutions and assessed for biofilm retention and bacterial survival. Antibiotic susceptibility profiling was performed and the ability of antimicrobial-impregnated silicone disks and Streptococcus salivarius probiotic strains K12 and M18 to inhibit the pathogens was investigated.

Results: Silicone oil effectively disrupted HA biofilms but did not affect …

Modelling The Growth Inhibition Of Common Food Spoilage And Pathogenic Micro-Organisms In Presence Of Solvent Extract From Irish York Cabbage, Shilpi Gupta, Amit Jaiswal, Nissreen Abu-Ghannam

Articles

Preservatives are required to maintain the quality, extend shelf life, and ensure safety of fresh and processed food products. Although chemical preservatives form an essential part in food preservation, legislation has restricted their use in different foods. Vegetables in the Brassica family (Cabbage, Broccoli, Brussels sprouts) are a rich source of a number of bioactive compounds such as flavonoids, glucosinolates and their breakdown products which may have antimicrobial, antioxidant and anticancer properties. The present study investigates the antimicrobial activities of solvent extract from Irish York cabbage, Brocolli and Brussels Sprouts on the growth inhibition of common food spoilage (Listeria monocytogenes …

The Legal Ecology Of Resistance: The Role Of Antibiotic Resistance In Pharmaceutical Innovation, Kevin Outterson

Faculty Scholarship

Antibiotic effectiveness is a common pool resource that can be prematurely depleted through resistance. Some experts warn that we may face a global ecological collapse in antibiotic effectiveness. Conventional wisdom argues for more intellectual property rights to speed the creation of new antibiotics. Recent theoretical literature suggests that conservation-based approaches may yield superior results. This Article describes a novel typology for organizing these emerging theories, and provides an early empirical test of these models, using proprietary data on the sales of vancomycin, an important hospital antibiotic for the last three decades.

The results challenge the assumptions in several models, and …

Inhibitors Of Bacterial N-Succinyl-L,L-Diaminopimelic Acid Desuccinylase (Dape) And Demonstration Of In Vitro Antimicrobial Activity, Danuta Gillner, Nicola Armoush, Richard C. Holz, Daniel P. Becker

Chemistry: Faculty Publications and Other Works

The dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE) is a critical bacterial enzyme for the construction of the bacterial cell wall. A screen biased toward compounds containing zinc-binding groups (ZBG’s) including thiols, carboxylic acids, boronic acids, phosphonates and hydroxamates has delivered a number of micromolar inhibitors of DapE from Haemophilus influenzae, including the low micromolar inhibitor l-captopril (IC50 = 3.3 μM, Ki = 1.8 μM). In vitro antimicrobial activity was demonstrated for l-captopril against Escherichia coli.

Inhibitors Of Bacterial N-Succinyl-L,L-Diaminopimelic Acid Desuccinylase (Dape) And Demonstration Of In Vitro Antimicrobial Activity, Danuta Gillner, Nicola Armoush, Richard C. Holz, Daniel P. Becker

Chemistry Faculty Research and Publications

The dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) is a critical bacterial enzyme for the construction of the bacterial cell wall. A screen biased toward compounds containing zinc-binding groups (ZBG’s) including thiols, carboxylic acids, boronic acids, phosphonates and hydroxamates has delivered a number of micromolar inhibitors of DapE from Haemophilus influenzae, including the low micromolar inhibitor L-captopril (IC₅₀ = 3.3 μM, K_i = 1.8 μM). In vitro antimicrobial activity was demonstrated for l-captopril against Escherichia coli.

Studies On The Antimicrobial Properties And Phytochemical Screening Of Garlic (Allium Sativum) Extracts, M. J. Olusanmi, J. E. Amadi

Ethnobotanical Leaflets

This study was carried out to evaluate the antimicrobial properties of garlic (Allium sativum) extracts on three fungi namely Aspergillus flavus, Curvularia lunata and Fusarium moniliforme using the pour plate method. A phytochemical screening of the extracts was also carried out to determine the constituents in garlic. Water, ethanol and acetone were the extractants used. Results showed that radial growth in all the three test organisms was impaired by the addition of the extracts in the culture medium used. The test organisms differed in their reaction to the different extracts but on the whole, growth inhibition increased with the concentration …

In-Vitro Evaluation Of Anti-Bacterial And Anti-Fungal Activity Of Vitex Nigundo (Verbenaceae), P. B. Aswar, S. S. Khadabadi, B. S. Kuchekar, R. M. Rajurkar, S. S. Saboo, R. D. Javarkar

Ethnobotanical Leaflets

Vitex negundo belongs to the family Verbenaceae. It is a large aromatic shrub distributed throughout the greater part of India up to an altitude of 1500 m in the outer Himalayas. It is widely planted as a hedge plant along the roads and between the roads. Traditionally it is having the flok claims like useful in treatment of rheumatism, insecticidal, antimicrobial, anticancer, tranquillizer, tonic, febrifuge, expectorant and diuretic properties. In the present study an attempt had been made to evaluate comparative antibacterial and antifungal principles from Vitex nigundo with some therapeutically used antibiotics. Different extracts of Vitex negundo leaves were …

Death From The Public Domain?, Kevin Outterson

Faculty Scholarship

In his recent article in the Texas Law Review, Ben Roin advances the claim that pharmaceutical innovation and the public’s health are harmed by the doctrines of non-obviousness and novelty. He does not mince words, labeling the nonobvious requirement as “perversity” with a “pernicious” effect on drug development. In his view, these standards pose an insurmountable barrier for drug companies seeking to commercialize inventions already in the public domain. He claims that valuable, life-saving drug ideas languish in the public domain because the companies face high barriers to entry from the FDA, but potential free riders are encouraged through the …

Cationic Polypeptides Contribute To The Anti-Hiv-1 Activity Of Human Seminal Plasma, Julie A. Martellini, Amy L. Cole, Nitya Venkataraman, Gerry A. Quinn, Pavel Svoboda, Bhushan K. Gangrade, Jan Pohl, Ole E. Sorensen, Alexander M. Cole

Public Health Resources

Mucosal surfaces of the reproductive tract as well as their secretions have important roles in preventing sexual transmission of HIV-1. In the current study, the majority of the intrinsic anti-HIV-1 activity of human seminal plasma (SP) was determined to reside in the cationic polypeptide fraction. Antiviral assays utilizing luciferase reporter cells and lymphocytic cells revealed the ability of whole SP to prevent HIV-1 infection, even when SP was diluted 3200-fold. Subsequent fractionation by continuous flow acid-urea (AU)- PAGE and antiviral testing revealed that cationic polypeptides within SP were responsible for the majority of anti-HIV-1 activity. A proteomic approach was utilized …

Antimicrobial Activity Of Plant Essential Oils Using Food Model Media: Efficacy, Synergistic Potential And Interaction With Food Components, Jorge Gutierrez, Catherine Barry-Ryan, Paula Bourke

Articles

The aim of this study was to optimise the antimicrobial efficacy of plant essential oils (EOs) for control of Listeria spp. and spoilage bacteria using food model media based on lettuce, meat and milk. The EOs evaluated were lemon balm, marjoram, oregano and thyme and their minimum inhibitory concentrations (MIC) were determined against Enterobacter spp., Listeria spp., Lactobacillus spp., and Pseudomonas spp. using the agar dilution method and/or the absorbance based microplate assay. MICs were significantly lower in lettuce and beef media than in TSB. Listeria strains were more sensitive than spoilage bacteria, and oregano and thyme were the most …

Antibacterial Activities Of Extracts From Some Turkish Endemic Plants On Common Fish Pathogens, Hakan Türker, Arzu Bi̇ri̇nci̇ Yildirim, Fatma Pehli̇van Karakaş, Hi̇lal Köylüoğlu

Turkish Journal of Biology

Antibacterial activities of 24 alcoholic and aqueous extracts from 8 endemic Turkish plants (Crocus abantensis, Crocus ancyrensis, Galanthus plicatus subsp. byzantinus, Paronychia chionaea, Astragalus gymnolobus, Trifolium pannonicum subsp.elongatum, Eryngium bithynicum, and Convolvulus galaticus) of 7 different families were screened. Antibacterial activity was carried out with 5 different fish pathogens (Aeromonas hydrophila, Yersinia ruckeri, Streptococcus agalactia, Lactococcus garvieae, and Enterococcus faecalis). Crude extracts of endemic plant extracts were applied against the bacteria using the disc diffusion method under in vitro conditions. A. hydrophila was the only inhibited bacteria from all alcoholic and aqueous extracts of C. ancyrensis, G. plicatus, T. pannonicum, …

Biological Screening Of Vitis Vinifera L. Leaf Fractions, Di̇dem Deli̇orman Orhan, Ni̇lüfer Orhan, Berri̇n Özçeli̇k, Fatma Ergun

Turkish Journal of Biology

The fractions of different polarity, namely chloroform, ethylacetate, n-butanol, and remaining water fractions, were fractionated from an aqueous extract of V. vinifera leaves. The antibacterial, antifungal, antiviral, antioxidant activities, and total phenolic content of these fractions were investigated. The chloroform fraction showed a promising effect against Herpes simplex virus type-1 (HSV-1) and Parainfluenza viruses (PIV). The results indicated that all the fractions were more pronounced against gram-positive bacteria (S. aureus and E. faecalis) than against gram-negative bacteria (E. coli and P. aeruginosa). On the other hand, the fractions showed equal antifungal activity against the genus Candida (C. albicans and C. …

The Chemical Ecology Of Antarctic Sponges, Kevin J. Peters

All ETDs from UAB

Sponges were collected from shallow waters along the western Antarctic Peninsula near Palmer Station on Anvers Island. The majority (78%) of the sponges were found to have outer tissues defended against the omnivorous, Antarctic sea star Odontaster validus. Of the species that had outer tissues defended, 62% of them also had internal tissues that inhibited feeding by O. validus. Lipophilic or hydrophilic extracts coated on artificial food pellets were found to be unpalatable for all of the sponge species tested. These data provide evidence that defenses are common survival strategies in sessile macroinvertebrates from Antarctica but that the allocation of …

Antimicrobial Effect Of Yogurt Lactic Acid Bacteria And Muscadine Products On Enterobacter Sakazakii, Weiien Weng

Theses and Dissertations

Enterobacter sakazakii has been associated with powdered infant formula outbreaks which caused high mortality rate illnesses in infants in recent years. Current research was mainly focused on searching for natural antimicrobial agents which may be incorporated into baby foods to control this emerging pathogen. Yogurt and muscadine products were used in this study. The antimicrobial effects of yogurt were evaluated on agar plates and in a simulated gastrointestinal model. In the agar spot tests, diluted yogurt sample containing lactic acid bacteria at 106 CFU/mL was antagonistic toward E. sakazakii. However, the antimicrobial effect of yogurt on E. sakazakii in the …

Design And Synthesis Of Carbohydrate Based Derivatives As Antimicrobial Compounds, Aoife Smith

Doctoral

Investigations into the design of analogues of GlcNAc-Ins, the substrate for the enzyme GlcNAc-Ins deacetylase (mshB), a therapeutic target on the pathway to mycothiol biosynthesis in Mycobacterium tuberculosis are described. Initial studies directed towards the design of a substrate analogue were based on the 3-D structure and a proposed mechanism of action of mshB (deduced by Dr. Andrew McCarthy, EMBL). The compounds were designed with the aim to produce an analogue which could better mimic the natural substrate for mshB (GlcNAc-Ins) for crystallisation and mechanistic studies to further improve the knowledge of this enzyme. A series of fatty acid ester …

Steam, Dielectric Heating And Copper Sulfate Treatment Of Inshell Pecans, Mandar Ranchhod Patel

Theses and Dissertations

Water and a copper sulfate (CSL) solution at 27, 57 and 88°C; steam pasteurization and dielectric heating were analyzed on their effect in reducing microbial load (APC) and Salmonella Typhimurium on inshell pecans (Carya illinoinensis). The CSL solution (more effective than water alone) reduced APC by 1.5 (300 s), 2 (60 s) and 4.0 (60 s) log CFU/g at 27, 57 and 88°C, respectively; and Salmonella by 3.0 log at 27°C in 60 s. Steam reduced APC by 3.7 log in 180 s and Salmonella by 4.0 log CFU/g in 30 s. Dielectric treatment reduced APC by 3.0 log and …

The Anti-Microbial Efficacy Of Plant Essential Oil Combinations And Interactions With Food Ingredients, Jorge Gutierrez, Catherine Barry-Ryan, Paula Bourke

Articles

The objective of this study was to evaluate the efficacy of plant essential oils (EOs) in combination and to investigate the effect of food ingredients on their efficacy. The EOs assessed in combination included basil, lemon balm, marjoram, oregano, rosemary, sage and thyme. Combinations of EOs were initially screened against Bacillus cereus, Escherichia coli, Listeria monocytogenes and Pseudomonas aeruginosa using the spot-on-agar test. The influence of varying concentrations of EO combinations on efficacy was also monitored using E. coli. These preliminary studies showed promising results for oregano in combination with basil, thyme or marjoram. The checkerboard method was then used …

Uji Aktivitas Antimikroba Dan Uji Sitotoksik Ekstrak Etanol Akar Tanaman Akar Kucing (Acalypha Indica Linn), Daging Buah Mahkota Dewa (Phaleria Macrocarpa (Sheff) Boerl) Dan Sari Buah Merah (Pandanus Conoideus Lam), Maksum Radji, Ratna Chandra Sari, Atiek Sumiati

Majalah Ilmu Kefarmasian

The antimicrobial activity and cytotoxic effect of ethanol extract of Acalypha indica Linn, Phaleria macrocarpa (Sheff) Boerl and Pandanus conoideus Lam, had been carried out. The results of the research showed that those ethanol extracts had antimi-crobial activities against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosae ATCC 27853 and Candida albicans. The determi-nation of cytotoxic effects of those ethanol extracts by brine shrimp lethality test (BSLT) using Artemia salina Leach showed that LC50 of Acalypha indica Linn, Phaleria macrocarpa (Sheff) Boerl and Pandanus conoideus Lam were 1,279 ug/ml, 0.123 ug/ml and 0.054 ug/ml respectively.

Food Colloids As Carrier Systems For Antimicrobials, Sarisa Suriyarak

Masters Theses 1911 - February 2014

Colloidal dispersions such as oil-in-water or water-in-oil emulsions have found widespread use in the food industry. Oil-in-water emulsions consist of three principal components i.e. oil dispersed in the form of droplets, water surrounding the droplets as the continuous phase, and emulsifiers comprising the interface. Because of the complicated interaction among components, it is often difficult to predict the physicochemical properties and final functionalities of emulsions. Nevertheless, the structural and functional features of emulsions allow scientists to create many unique emulsions that may serve as suitable carriers for lipophilic functional compounds. These functional compounds may include antioxidants, flavors, colors and antimicrobials, …

Questioning The Justifiability Of Innovation Protection In Antimicrobial Drugs: A Law And Economics Perspective, Ankur Sood, Vardaan Ahluwalia

Northwestern Journal of Technology and Intellectual Property

No abstract provided.

Regulation Of The Lactoperoxidase System In The Airway, Miryam A. Fragoso

Open Access Dissertations

The lactoperoxidase (LPO) antimicrobial system has been shown to play an important role in maintaining sterile conditions in several tissues including the mammary gland, the salivary gland, and the airway. The LPO system in the airway consists of the enzyme LPO and its substrates hydrogen peroxide and an anion. LPO catalyzes the oxidation of a halide or pseudohalide ion for example SCN-or I- by hydrogen peroxide producing a product, OSCN- or OI- which have antibacterial, antifungal, and antiviral properties. In order to have a functional antimicrobial system all the components need to be present at appropriate concentrations. The LPO system …

The Antibacterial Properties Of Brookite Phase Titanium Dioxide Nanoparticles Against Methicillin-Resistant Staphylococcus Aureus, Rupal Shah

All Theses

Staphylococcus aureus (S. aureus), a major human pathogen, is a common cause of infections worldwide due to its high virulence intensity. By adapting to rapidly changing and uniformly hostile environments, strains of S. aureus acquire resistance to antimicrobial agents shortly after their exposure. For example, within a year of its introduction, S. aureus developed resistance to methicillin which triggered the development of other antimicrobial treatments. In spite of the various antibiotics currently used to treat methicillin-resistant S. aureus (MRSA) infections, antimicrobial resistance is an unavoidable consequence due to the selective pressure of antibiotic exposure. Thus, other prevention modalities are warranted …

Colloidal Particles As Antimicrobial Carrier Systems, Dustin W. Carnahan

Masters Theses 1911 - February 2014

The objective of this thesis is to develop a method by which antimicrobials are delivered into a food product as a concentrated dose to the specific area in which the microorganism is growing without interference from the food matrix. More specifically, we plan to achieve this by delivering the antimicrobials nisin and lysozyme attached to nanoparticles and emulsion droplets. We hypothesize that (a) the attachment to a delivery vessel may increase the local concentration of the antimicrobial in the vicinity of the bacterial pathogens and (b) that the size and charge of the nanoparticle following attachment of the antimicrobials will …

Gyra Mutations In Fluoroquinolone Resistant Clostridium Difficile Pcr 027, Denise Drudy, Lorraine Kyne, Rebecca O’Mahony, Séamus Fanning

Articles

No abstract provided.

Evaluating The Effect Of Two Commercial Antimicrobial Products On Salmonella Spp. In The Aquatic Habitat Of The Red-Eared Slider Turtle (Trachemys Scripta Elegans), Trevor Theadore Zachariah

LSU Master's Theses

Turtle-associated salmonellosis was recognized as a public health concern in the 1960’s, particularly due to an increase in the incidence of disease among children. In response to the public health threat, the United States Food and Drug Administration (FDA) implemented regulations in 1975 restricting the sale of turtle eggs and turtles with a carapace length less than 10.2 cm. Since that time, attempts to eliminate Salmonella from turtles using antibiotics have been unsuccessful and lead to antibiotic resistant strains of Salmonella on turtle farms. Recent work has focused on identifying non-antibiotic products to suppress or eliminate Salmonella and reverse the …

Antimicrobial Properties Of Syringopeptin 25a And Rhamnolipids, Prerak T. Desai

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The increasing bacterial resistance to available antibiotics requires the search for new antibacterial compounds to be broadened. This study investigated the antimicrobial properties of two secondary metabolites from fluorescent pseudomonads -- syringopeptin 25A, a lipodepsipeptide produced by Pseudomonas syringae pv. syringae, and a rhamnolipid mixture produced by Pseudomonas aeruginosa. The rate of antimicrobial action was determined by monitoring the rate of uptake of propidium iodide during exposure to the compounds. Inhibition was also confirmed by the microbroth dilution method to determine the MI Cs. Both the compounds inhibited growth of Gram-positive organisms, including Mycobacterium smegmatis, staphylococci, and …

Antimicrobial Effects Of Copper And Brass Ions On The Growth Of Listeria Monocytogenes At Different Temperatures, Ph And Nutrients, Aisha Abushelaibi

LSU Doctoral Dissertations

Listeria monocytogenes has been recognized as a human pathogen since 1929. This pathogen is found in many foods and listeriosis infections affect approximately 2,500 people in the United States each year, according to the Centers for Disease Control and Prevention. Of those infected with L. monocytogenes approximately 500 die as a result of the illness. Listeria monocytogenes is a bacterium, commonly found in water, soil, plant material, animals and human. Today, different methods are used by food manufacturers, to reduce the risk of Listeria monocytogenes, such as antimicrobial agents, heating, irradiation, and fermentation. The ability of the bacteria to grow …

Antimicrobial Effect Of Cetylpyridinium Chloride Against Listeria Monocytogenes Growth On The Surface Of Raw And Cooked Shrimp, Tracie Michelle Dupard

LSU Master's Theses

Listeria monocytogenes has emerged as a major foodborne pathogen for the seafood industry due to its psychrotrophic nature and its ubiquitous presence. It has been isolated from soil, sewage, dead vegetative matter, aquatic environments, fecal material, fish, crustaceans, and domesticated animals. As a result, L. monocytogenes has been responsible for several shrimp recalls and has been epidemiologically linked to human listeriosis. Fresh seafood products are highly perishable and their shelf-life is limited by microbiological spoilage. Therefore, when pathogenic microorganisms are involved, it poses a health threat to the general public. The situation is further complicated because seafood processing plants are …

Pharmacokinetics Of Once-Daily Amikacin In Healthy Foals And Therapeutic Drug Monitoring In Hospitalized Equine Neonates, Erica Paige Bucki, Steeve Giguère, Margo Macpherson, Rachel E. Davis

Faculty Publications

The objectives of this study were to investigate the pharmacokinetics of once-daily amikacin in healthy neonates, to determine amikacin concentrations in hospitalized foals, and to determine the minimum inhibitory concentrations (MICs) of amikacin against gram-negative isolates from blood cultures in septic foals. Median half-life, clearance, and volume of distribution of amikacin in healthy 2- to 3-day-old foals after administration of an intravenous bolus of amikacin (25 mg/kg) were 5.07 hours (4.86-5.45 hours), 1.82 mL/min/kg (1.35-1.97 mL/min/kg), and 0.785 L/kg (0.638-0.862 L/kg), respectively. Statistically significant (P or = 3 microg/mL between the 2 groups. An initial dose at 25 mg/kg is …