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A Temperature-Sensitive Mutant Of Escherichia Coli Affected In The Alpha Subunit Of Rna Polymerase, Majid Mehrpouyan

Electronic Theses and Dissertations

A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase has been investigated. Gene mapping and complementation experiments placed the mutation to temperature-sensitivity within the alpha operon at 72 min on the bacterial chromosome. The rate of RNA synthesis in vivo and the accumulation of ribosomal RNA were significantly reduced in the mutant at 44$\sp\circ$C. The thermostability at 44$\sp\circ$C of the purified holoenzyme from mutant cells was about 20% of that of the normal enzyme. Assays with T7 DNA as a template showed that the fraction of active enzyme competent for transcription was reduced as a …

The Differences Between Industrial And Municipal Executives' Practices And Perceptions Of An Npdes Water Pollution Control System In Tennessee, Anthony L. Fulkerson

Electronic Theses and Dissertations

The purpose of this study was to determine the differences between industrial and municipal executives' practices and perceptions toward the administrative requirements of a water pollution control system as set forth in a National Pollutant Discharge Elimination System permit. This was a descriptive study that utilized a two-part attitudinal survey designed to obtain the perceptions of the executives (N = 171) of all major industries and all major municipalities in the State of Tennessee who had direct responsibility for the administrative requirements of an NPDES permitted water pollution control system. Descriptive statistics were used for the analysis of data with …

Electrophysiology, Cell Calcium, And Mechanisms Of Hepatocyte Volume Regulation, Walid E. Khalbuss

Electronic Theses and Dissertations

The electrophysiologic technique (Reuss, L., Proc. Natl. Acad. Sci. USA 82:6014, 1985) was modified to measure changes in steady-state hepatocyte volume during osmotic stress. Hepatocytes in mouse liver slices were loaded with tetramethylammonium ion (TMA$\sp{+}$) during transient exposure of cells to nystatin. Intracellular TMA$\sp{+}$ activity (a$\sp{\rm i}\sb{\rm TMA}$) was measured with TMA$\sp{+}$-sensitive, double-barreled microelectrodes. Loading hepatocytes with TMA$\sp{+}$ did not change their membrane potential (V$\sb{\rm m}$), and under steady-state conditions a$\sp{\rm i}\sb{\rm TMA}$ remained constant over 4 min in single impalements. Hyperosmotic solutions (50, 100, & 150 mM sucrose added to media) and hyposmotic solutions (sucrose in media reduced by …

The Rise And Fall Of The Great Music Festivals And The Hippie Culture Of The 1960’S: Monterey, Woodstock, And Altamont, Charles Anthony Gates

Electronic Theses and Dissertations

[No abstract provided.]

A Comparison Of The Effectiveness Of Civilian Conservation Centers And Contract Centers From 1984 To 1988, Robert P. Slaughter

Electronic Theses and Dissertations

The purpose of this study was to determine whether the continuous instability of policy decisions relating to the effectiveness of Civilian Conservation Centers and Contract Centers was a result of measurable differences in performance between the two models. Six hypotheses tested differences between types of centers on six quantitative variables which were: average length of stay, job placement, hourly starting wages, educational gains in reading, math, and General Educational Development (GED). To investigate the problem, 30 Civilian Conservation Centers and 17 comparably sized Contract Centers were selected. Reports from the Department of Labor's Automated Management System provided data for comparison …

Minerva At Large: A Study Of The Leadership Styles And Educational Philosophies Of The Department Of Education Secretaries, Mark Y. Herring

Electronic Theses and Dissertations

In 1979, President James E. Carter signed into law the thirteenth Cabinet-level office, the Department of Education. Although the United States has had a department, bureau, or office of education since 1867, the newly established Department of Education gave education a seat at the Cabinet table. Since that time, four individuals have served as secretaries: Shirley M. Hufstedler, Terrel H. Bell, William J. Bennett, and Lauro F. Cavazos. Examined in this study were the importance of leadership styles and educational philosophies as exhibited by the four secretaries. A tertiary concern viewed the role of the federal government in education. Leadership …

Knowledge Of And Response To Copyright Law, School Copyright Policy, And Copyright-Related Issues: Survey Of Secondary School Principals And Librarians, Koleta B. Tilson

Electronic Theses and Dissertations

The problem of this study was that, with the impact of new media and delivery systems, principals and librarians must respond to copyright issues in order to remain informed about the copyright law and the legal use of media. The purpose of this study was to gather and evaluate educator response to issues related to copyright. The study was conducted with a sample of regionally accredited secondary schools in the following states: Alabama, Florida, Georgia, Kentucky, Louisiana, Mississippi, North Carolina, South Carolina, Tennessee, Texas, and Virginia. A total of 1008 questionnaires were mailed to the principal and the librarian of …

The Relationship Of Personality Type To Leader Style And Perceived Effectiveness Among Dental Hygiene School Administrators, Susan J. Willette

Electronic Theses and Dissertations

The purpose of this study was to identify personality types among dental hygiene school administrators and faculty and to determine if correlations existed between leader style, ideologies of leader style, perceived effectiveness, and personality type. Selected demographic variables were also examined. The dimensions of personality investigated were derived from the Myers-Briggs Type Indicator: extroversion, introversion, sensing, intuition, thinking, feeling, judging, and perceiving. The leadership behavior dimensions were the two dimensions of the real and ideal Leadership Behavior Description Questionnaires: consideration and initiating structure. Effectiveness was measured by a 39 item Likert-type instrument based on Tucker's (1981) categorical listing of chairperson …

Dopamine Agonists Modify The Development Of Brain D1 And D2 Receptor Responsiveness, Anwar A. Hamdi

Electronic Theses and Dissertations

Dopamine (DA) agonist-induced behavioral supersensitivity in the adult rat has served as the standard model for certain of the motor and behavioral side effects associated with long-term exposure to DA agonists in humans. The mechanisms relating receptor events with behavior mediation, however, remain unclear. The striatum of rats progresses through a prolonged and varied postnatal developmental period. In order to examine the relative contribution of D1 and D2 receptor-mediated mechanisms to behavioral changes which follow chronic dopamine agonist exposure, developing rats were treated daily from birth with a D1 agonist, SKF 38393 hydrochloride (3.0 mg/kg $\times$ 32d, i.p.), or a …

The Use Of Orientalism In The Drama Of Elizabeth Inchbald, Valerie Denise Adams

Electronic Theses and Dissertations

Throughout the eighteenth century, interest in the East continued to flourish and incentives to travel there increased. As colonial expansion grew, the middle class began touring abroad with their entire families. Returning home full of excitement from their adventures in exotic places, they felt compelled to publish travel books and to share what they had seen. The materials and interpretations of the East that appeared in the travel books captured the imagination of numerous writers in England and created a large body of literature in which the Orient was the background.

One of these writers was Elizabeth Simpson Inchbald. This …

The Effect Of Repetitive Midazolam Treatment On Acoustical Startle In Rats, Robert Francis Mirabella

Electronic Theses and Dissertations

Benzodiazepines are a class of drugs that have several actions including hypnotic features, anti-convulsant features, and anxiolytic features. Midazolam (MZ), a short acting benzodiazepine, is of special interest because of its probable high abuse potential and its efficacy as a preanesthetic.

Tolerance to drug effect has been found for both the hypnotic and anti-convulsant activity of benzodiazepines. Tolerance to the anxiolytic activity of benzodiazepines, however, is less clear. Evidence suggests that the time course for the development of tolerance associated with the anxiolytic action of MZ is slower than the time course associated with tolerance to the sedative and anticonvulsant …

Dopamine Agonists Modify The Development Of Brain D1 And D2 Receptor Responsiveness, Anwar A. Hamdi

Electronic Theses and Dissertations

Dopamine (DA) agonist-induced behavioral supersensitivity in the adult rat has served as the standard model for certain of the motor and behavioral side effects associated with long-term exposure to DA agonists in humans. The mechanisms relating receptor events with behavior mediation, however, remain unclear. The striatum of rats progresses through a prolonged and varied postnatal developmental period. In order to examine the relative contribution of D1 and D2 receptor-mediated mechanisms to behavioral changes which follow chronic dopamine agonist exposure, developing rats were treated daily from birth with a D1 agonist, SKF 38393 hydrochloride (3.0 mg/kg $\times$ 32d, i.p.), or a …

Purification And Characterization Of Enoyl-Acp Reductase From Euglena Gracilis, Margie M. Tucker

Electronic Theses and Dissertations

Enoyl-(acyl-carrier-protein) reductase was purified from the phytoflagellate Euglena gracilis. Its purification employed DEAE-Sephacel chromatography, Matrex Orange chromatography, and affinity chromatography using acyl carrier protein (ACP) covalently bound to Sepharose as the affinity ligand. Matrex Orange chromatography resolved two different enoyl-ACP reductases having different characteristics. Euglena gracilis appears to resemble higher plants in the possession of two isoforms of this enzyme. Antibodies specific for the cofactor binding site of NADP (H)-requiring dehydrogenases were obtained. They were isolated from a polyclonal population of antibodies directed against yeast glucose-6-phosphate dehydrogenase by affinity chromatography using chicken liver malic enzyme as the affinity ligand. The …

Teachers’ Perceptions Of Principal Effectiveness In Selected Secondary Schools In Tennessee, Henry S. Williams

Electronic Theses and Dissertations

The purpose of this study was to compare teachers' perceptions of principal effectiveness in secondary schools nominated for the National Secondary School Recognition Program and a randomly selected sample of schools not nominated for the National Secondary School Recognition Program in Tennessee. Evaluation of principal effectiveness was based upon teachers' perceptions as measured by the Audit of Principal Effectiveness, (APE). The data were gathered by mail survey. The Audit of Principal effectiveness were sent to participating secondary schools in the state of Tennessee with enrollment of 1000 and more. The teachers were asked to participate by answering a questionnaire regarding …

The Everyday World As Violent: A Feminist Exploration Of The Social Constructions Of Violent Female Offenders., Julie Catherine. Hunt

Electronic Theses and Dissertations

This research explores violent crimes by women utilizing an interactionist and feminist framework. Detailed interviews were conducted with women serving time for offenses of violence at Huron Valley Women's Facility; a maximum security penitentiary located in Ypsilanti, Michigan. In accordance with other interactionist research on violence, emphasis is on situational definitions and the impact of self concept on the development of violent interactions. This thesis attempts to move beyond the predominant theoretical models developed in this area by researchers such as Athens (1977; 1980), Dietz (1983), Felson (1978), Felson and Steadman (1983), and Luckenbill (1977) by stressing that the concept …

The Federal Government, Ontario And Medical Care Insurance: A Study In Federal-Provincial Relations., Stephen T. Holloran

Electronic Theses and Dissertations

This is a history of the medical care insurance system in Ontario. I undertook this study in order to help explain a part of Canadian history that has been severely neglected. This is an historical account of the events that led to Ontario's acceptance of the national medical care insurance plan, and the consequent introduction of the Ontario Health Services Insurance Plan (OHSIP). After introducing OHSIP (1969) Ontario soon amended the program to the current OHIP (1972). A history of these programs would certainly be a logical extension of this study. At one time or another medical care treatment is …

Ultimate Strength Of Schifflerized Angles., Seshu Madhava Rao. Adluri

Electronic Theses and Dissertations

Latticed triangular-base steel towers have been used as communication structures for a long time. Since these triangular-base towers are economical, they are also being increasingly used as electrical transmission line towers. The legs of these towers generally consist of 60$\sp\circ$ equal leg angles (either 90$\sp\circ$ rolled angles bent to 60$\sp\circ$ or rolled 60$\sp\circ$ angles) which are primarily compression members. Results of experimental investigation on eighteen equal-leg schifflerized angles (90$\sp\circ$ angles bent to 60$\sp\circ$) under concentric axial compressive loading with hinge-hinge end conditions are presented. Five sizes of angles viz. 5 x 5 x 5/16, 4 x 4 x 1/4, 3$1\over2$ …

Values Projected In Native-Oriented National Film Board Of Canada Productions., David Andrew. Brown

Electronic Theses and Dissertations

This study examined the portrayal of native Canadians in films of the National Film Board of Canada. The issue was cast in the context of the historical conflicts between the federal government and aboriginal people in Canada over issues of power, culture, language, economics, spirituality, land claims and the environment. It also examined how this adversarial relationship affects, and is affected by, past and present public opinion and past and present media portrayals of natives. Because the literature suggests that the dominant values of the society of which a film-maker is a member will be projected in his or her …

Vocational Bias: Students Entering Nontraditional Careers., Brenda A. Tomini

Electronic Theses and Dissertations

Vocational bias can be defined as an implicit assumption that certain careers are more appropriate for one gender than another. Study 1 examined vocational bias from a student's perspective. More specifically, this study addressed the careers to which Canadian undergraduates currently aspire, individuals who influence these choices, students' career confidence, and principally, recent experiences with high school counsellors. In particular, the occupational choices of female students were focused on, especially in the context of previous experience with counsellors. As well, the types of extracurricular activities students participate in were examined. Study 2 examined vocational bias from the perspective of teachers. …

A Computer Model For The Management Of Contaminant Plumes And Site Dewatering., Robert J. Maclean Hyde

Electronic Theses and Dissertations

The computer model presented here utilizes a finite difference approximation of the groundwater flow continuity equation in an aquifer to predict water table elevations and the Todd (1980) equation to calculate velocity dependent capture zones for pumping wells. The model presented in this thesis has a series of wells, with variable pumping rates, advance through designated areas of a finite difference grid system discretizing the aquifer. Wells advance through the grid to new nodal configurations and an optimum well configuration solution is found when prescribed conditions are fulfilled. In a dewatering simulation, these conditions are in the form of predesignated …

Second Phase Particles In Zirconium Alloys., Xianying Meng

Electronic Theses and Dissertations

Transmission electron microscopy, scanning electron microscopy, energy dispersive x-ray spectroscopy, and x-ray diffraction have been used to study the zirconium alloys, Zr-1wt%Nb. Zr-2.5wt%Nb, Zircaloy-2, Zircaloy-4, and bulk stoichiometric $\rm Zr(Fe\sb{1-x}Cr\sb{x})\sb2$ alloys, where x = 0, 0.25, 0.45, and 0.75. Both Zr-2.5Nb alloy pressure tubing and Zr-1Nb fuel sheathing contain two main phases, $\alpha$-Zr and $\beta$-Zr. Zr(FeCr)$\sb2$ Laves phase precipitates and Zr$\sb3$Fe intermetallic phase were found in cold-worked Zr-1Nb alloy. Only Zr$\sb2$Fe precipitates were found, however, in Zr-2.5Nb pressure tubing, which contains more Fe and Cr than Zr-1Nb. The volume fraction of Zr$\sb2$Fe was much smaller than the volume fraction of …

Sulfhydryl Modification Of The Calmodulin-Stimulated Phosphatase, Calcineurin., Jerome David. Wagner

Electronic Theses and Dissertations

Calcineurin (CaN), a calmodulin stimulated protein phosphatase is a heterodimer composed of a 61kD catalytic subunit and a 19kD regulatory subunit. The larger subunit has three binding sites for transition metal ions and also contains the intrinsic metal ions, Zn$\sp{2+}$ and Fe$\sp{3+}$. The smaller subunit contains four "EF-hand" Ca$\sp{2+}$ binding sites. All ten of calcineurin's cysteine residues are found on the catalytic subunit. This study explores the metal ion dependence of CaN using Ca$\sp{2+}$, Mg$\sp{2+}$, Mn$\sp{2+}$ and Ni$\sp{2+}$ as well as its thiol chemistry using Ellman's reagent, iodoacetate, iodoacetamide, glutathione and a novel thiol reagent introduced by this study, 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one …

Surface Enhanced Spectroscopy Of Perylene Derivatives., Urmi. Guhathakurta

Electronic Theses and Dissertations

Surface enhanced Raman (SERS), surface enhanced Resonance Raman (SERRS) of mono and multilayer films of three N,N$\sp\prime$-unsymmetrical dialkyl-3,4:9,10-perylenebis (dicarboximides)s, namely N-octyl-3,4:9,10-perylenetetracarboxylic monoanhydride(Oc-PTCDO), N-octyl-3,4:9,10-perylenetetracarboxylic monoimide(Oc-PTCDNH) and N-octyl, N$\sp\prime$-isobutyl-3,4:9,10-perylene-bis (dicarboximide) (PBDC) have been studied and vibrational analyses have been attempted. SERS and SERRS of evaporated thin films of perylene, 3,4:9,10 perylene tetracarboxylic dianhydride (PTCDA) and 3,4:9,10 perylene tetracarboxylic N,N$\sp\prime$ bis (methyl) diimide (PTCDIMe) on tin sphere, Ag island and Au island films are used to characterize the vibrations of the three unsymmetrical perylene derivatives. It was concluded that the LB layers were physisorbed onto the SERS active surfaces. All three samples form …

An Examination Of The Force-Interval Relationship In Chronic Diabetic Rat Papillary Muscle., Tina Ann. Mccomb

Electronic Theses and Dissertations

The purpose of the present study was to fully characterize the force-interval relationship of the right ventricular papillary muscle from chronic diabetic rats, and, to more closely examine the alterations in the beta phase reported previously. The condition of chronic diabetes resulted in several alterations in the force-interval relationship, primarily in the beta phase of force development. Alterations in the percent contribution of beta to the total amplitude and the rate and time constants of the beta phase were identified and suggest alterations in the capacity of the diabetic tissue to transport calcium through the exchange compartment of the sarcolemma …

The Petrology And Geochemistry Of The Minor Intrusions In The Central Metasedimentary Belt, Grenville Province, Southern Ontario, Canada., Sarah E. Kennedy. Stanford

Electronic Theses and Dissertations

The study area lies within the western section of the Elzevir terrane, in the Central Metasedimentary Belt. A supracrustal sequence of carbonates and clastic metasediments, interdigitating with metavolcanics occur in this area, intruded by a series of dykes. The majority of minor intrusions are mafic in composition; a minor number of felsic samples have been identified. The dykes have been metamorphosed to middle and upper amphibolite facies of low-pressure type, transitional into moderate pressures. Major and trace element contents were determined by X-Ray Fluorescence Analysis. Four distinct geochemical suites have been recognized: a Low Zirconium Suite of tholeiitic basalts, an …

The Performance Of Language-Impaired Preschoolers On The K-Abc Scales., Jo-Ann Hilda. Birt

Electronic Theses and Dissertations

This study examined the effects of language impairment and age on the pattern of performance on the scales of the Kaufman Assessment Battery for Children (K-ABC). Four groups of preschool children were examined: 14 four-year-old children and 10 five-year-old children with a clinical diagnosis of expressive and receptive language impairments; 14 four-year-old children and 19 five-year-old children referred for reason other than language impairment. Subjects were matched on age, sex, SES, and presence of behavior problems. The results provided some support for Telzrow's (1984) predicted pattern of performance. The language-impaired children did perform better on the Simultaneous Processing than the …

The Role Of Epistemic Style In The Processing Of Style Consistent And Style Inconsistent Counterattitudinal Editorials., David Martin. Day

Electronic Theses and Dissertations

The present study examined epistemic style predispositions associated with individual differences in responsiveness to style-consistent and style-inconsistent persuasive communications. Two hundred and fifty-seven undergraduate students participated. In Session 1, subjects were given (a) a questionnaire measuring their attitudes toward two relevant issues, student access to course evaluations and equal automobile insurance rates for men and women in Ontario, (b) the Psycho-Epistemological Profile (PEP) to assess their characteristic epistemic styles (rational, empirical, or metaphorical), and (c) a brief demographic survey. In the second session, subjects in the experimental group (n = 200) read two counterattitudinal, persuasive editorials on the two issues …

The Selection Of Juries: Lawyers' Perspectives, Rudolf Kreis

Electronic Theses and Dissertations

Juries have been a long standing institution within the Canadian criminal justice system. However, there is to be found very little research in the area. This exploratory study seeks to examine the selection of juries from the lawyer's perspective, both Crown and defence, and the factors that the lawyers find as being significant to them. The research is conducted from a symbolic interactionist perspective of the study of the definition of the situation, of selecting a Jury for a criminal trial. The research methodology combines a multi-faceted approach which combines observations, interviews and questionnaires to arrive at its findings. It …

Predicting The Psychosocial Outcome Of Traumatic Brain-Injured Adults., Andree. Tellier

Electronic Theses and Dissertations

The serious nature of the psychosocial maladjustment of many traumatic brain-injured (TBI) patients has led researchers to attempt to predict the eventual level of recovery of these patients. The ability to predict the psychosocial adjustment would be invaluable for several reasons: it would allow professionals to provide patients with more accurate information concerning their likely level of recovery and also bring their family's expectations to realistic levels; it could also help professionals identify sooner patients in greatest need of counseling. Numerous studies have demonstrated the predictive superiority of neuropsychological variables over that of the initial indices of severity such as …

Preservice Teacher Beliefs Related To Mathematics And Language Arts., David W. Kellenberger

Electronic Theses and Dissertations

This study investigated the relationship between preservice teachers' mathematical and language arts learning histories and beliefs about their (1) attribution of students' achievement, (2) self-efficacy as future teachers, and (3) other subject-/topic-related perceptions. The belief most closely linked to the learning history was the self-efficacy as future teachers. The group with a less favourable mathematical learning history and a more favourable language arts learning history believed they were significantly less able to influence students' effort, interest, and achievement in mathematics compared to language arts. In addition, when groups were compared, preservice teachers with a less favourable mathematical learning history believed …