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Characterization Of Invariant Membrane Proteins Of Trypanosoma (Duttonella) Vivax, Barbara Anne Burleigh
Characterization Of Invariant Membrane Proteins Of Trypanosoma (Duttonella) Vivax, Barbara Anne Burleigh
Digitized Theses
Monoclonal antibodies (mAbs), raised against whole, fixed, uncoated, culture forms of Trypanosoma (Duttonella) vivax, were used to identify two invariant membrane proteins of this protozoan parasite. Since non-variant membrane proteins of the cell surface, flagellar pocket and endocytic pathway are potential targets for the control of trypanosomiasis of livestock by immunization, the identification and characterization of invariant membrane proteins is a necessary preliminary step.;A 65 kDA invariant membrane glycoprotein (gp65), identified using mAb 4E1, was the main focus of this study. Immunolocalization studies using the monoclonal antibody (mAb 4E1) for immunofluorescence staining and immunoelectron microscopy, demonstrated that the 65 kDa …
Host And Viral Determinants Influencing The Pathogenesis Of Coronavirus-Induced Neurological Disease In Rodents, John M. Pasick
Host And Viral Determinants Influencing The Pathogenesis Of Coronavirus-Induced Neurological Disease In Rodents, John M. Pasick
Digitized Theses
Host and viral determinants influencing the outcome of coronavirus JHM infections of the CNS of rodents were investigated using intact animals and primary neural cell cultures. Two problems pertinent to the process of pathogenesis were addressed. The first was concerned with elucidating host factors responsible for controlling the age dependent nature for induction of the demyelinating form of disease in suckling rats. In the second, the basis of the relative resistance exhibited by inbred SJL/J mice to JHMV was examined in relation to both host and viral determinants.;Using primary, dissociated neural cultures from perinatal rats, several interesting aspects of host …
Isolation And Characterization Of A Genetic Locus Involved In Growth And Pathogenicity On Tomato Plants By Pseudomonas Syringae Pv Tomato, Dennis Paul Jackson
Isolation And Characterization Of A Genetic Locus Involved In Growth And Pathogenicity On Tomato Plants By Pseudomonas Syringae Pv Tomato, Dennis Paul Jackson
Digitized Theses
Pseudomonas syringae pv. tomato is the causal agent of bacterial speck on tomato. An essential feature of this organism, in the pathogenic process, is its ability to enter the intercellular spaces of its host and use the available plant substrates for growth. Tomato leaves contain quantities of carbon energy in the form of carboxylates. A Tn5 mutant, DC3481, cannot utilize carboxylates as a source of carbon energy for growth (CA{dollar}\sp-{dollar}) and is nonpathogenic (Path{dollar}\sp-{dollar}) on tomato. The focus of this thesis was to utilize DC3481 to isolate and characterize P. syringae pv. tomato DNA sequences involved with growth and pathogenicity …