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Alcohol And Other Addictive Behaviors Among Bariatric Surgery Patients, Rachel M. Arnold

Theses and Dissertations

Research has repeatedly highlighted a high rate of comorbidity of addictive behaviors in both clinical and non-clinical samples. While polydrug use has received significant attention in recent years, less is known about rates of comorbidity with behavioral addictions such as eating, work, exercise, or sex. Individuals with a history of bariatric surgery may provide a unique opportunity to examine the potential co-occurrence of addictive behaviors. High rates of food addiction symptoms and changes in alcohol use patterns post-surgically have highlighted a potential gap in our understanding of bariatric patients and their needs post-surgically. Using a composite measure of addictive behaviors, …

Genetics And Alcohol Interventions In Youth, Zoe E. Neale

Theses and Dissertations

Alcohol is the most commonly used substance among youth, and risky alcohol use is associated with harmful consequences such as accidents, academic consequences, and physical and emotional health problems. Alcohol use disorders are approximately 50% heritable, yet most efforts to prevent and intervene upon youth alcohol use focus only on environmental factors. Furthermore, current prevention and intervention programs tend to have modest effects and are not uniformly effective for all individuals. Gene-by-intervention (GxI) studies offer an opportunity to expand current understanding of interventions by examining whether underlying genetic risk may contribute to differential program effects. Much of the current GxI …

Identifying Genes Downstream Of Mef2 That Influence Ethanol Sedation In Drosophila Melanogaster, Ananya Talikoti

Theses and Dissertations

Alcohol use disorder is a global public health issue that affects millions across the world. It can result in negative physical and mental health outcomes, and currently treatment options are limited and rates of relapse are high. Identifying genes that affect aspects of ethanol behaviors in model organisms, such as Drosophila melanogaster, can serve to eventually develop more robust therapeutic interventions for those experiencing alcohol dependence. Previous studies have identified a relationship between a person's initial sensitivity to alcohol and their abuse potential for the drug in later life. Therefore, we can study sedation behaviors in Drosophila melanogaster to …

Mef2-Bound Genes May Influence Ethanol Sedation In Drosophila Melanogaster, Katlyn M. Myers

Theses and Dissertations

Alcohol Use Disorder (AUD) is a global health issue that affects millions of people every year. This disorder has serious negative mental and physical consequences. Currently, treatment options for this disorder are largely limited to psychological therapy, with very few medications available to treat it. Being able to identify the environmental and genetic components that influence AUD can help improve diagnosis and treatment options. Previous studies in humans have shown a link between initial sensitivity and risk for alcohol abuse. Our laboratory uses Drosophila melanogaster as a model to study the genetic and environmental components of alcohol-related behaviors. Previous lab …

Transcriptomic Profiling Of Postmortem Prefrontal Cortex And Nucleus Accumbens From Chronic Alcohol Abusers., Eric S. Vornholt

Theses and Dissertations

Alcohol use disorder (AUD) is a debilitating psychiatric illness that develops from a combination of genetic and environmental factors. While it is well documented that AUD is heritable, the shift from recreational alcohol use to abuse/dependence is poorly understood. In this dissertation, using postmortem brain tissue from individuals with alcohol dependence (AD), we profiled the genome-wide expression of circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) to better understand the impact of gene expression on the development of AUD. To achieve this, we performed two independent studies that explore transcriptome differences between AD cases and controls. The first of …

Genome-Wide Systems Genetics Of Alcohol Consumption And Dependence, Kristin Mignogna

Theses and Dissertations

Widely effective treatment for alcohol use disorder is not yet available, because the exact biological mechanisms that underlie this disorder are not completely understood. One way to gain a better understanding of these mechanisms is to examine the genetic frameworks that contribute to the risk for developing this disorder. This dissertation examines genetic association data in combination with gene expression networks in the brain to identify functional groups of genes associated with alcohol consumption and dependence.

The first study took advantage of the behavioral complexity of human samples, and experimental capabilities provided by mouse models, by co-analyzing gene expression networks …

Glial Cell Mechanisms Regulate Alcohol Sedation In Drosophila Melanogaster, Kristen M. Lee

Theses and Dissertations

Approximately 16 million people in America are diagnosed with Alcohol Use Disorder (AUD) but no efficacious medical treatments exist. Alcohol-related behaviors can be studied in model organisms, and changes in these behaviors can be correlated with either (i) a risk for alcohol dependence or (ii) a symptom/feature of AUD itself. Although AUD is a disease of the central nervous system, a majority of research has focused on the neuronal underpinnings, leaving glial contributions largely undescribed. We used Drosophila melanogaster (fruit fly) to identify genes whose expression in glia regulates alcohol sedation. Mammals and Drosophila have conserved behavioral responses to alcohol …

Cellular Mechanisms By Which Alcohol Promotes Hiv Protease Inhibitor-Induced Hepatotoxicity, Michael Hinton

Theses and Dissertations

CELLULAR MECHANISMS BY WHICH ALCOHOL PROMOTES HIV PROTEASE INHIBITOR-INDUCED HEPATOTOXICITY

Michael Hinton, B.S.

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University

Virginia Commonwealth University, 2019

Major Director: Huiping Zhou

Professor, Department of Microbiology and Immunology

The development of highly-active-antiretroviral therapy(HAART) has allowed management of HIV and extended the lives of those infected. Alcohol abuse, which is very common in HIV-1 infected patients, is one of the most important co-morbid risk factors for liver injury and has been associated with the occurrence of serious metabolic syndrome and subsequent discontinuation …

Role Of Mitochondrial Beta-Oxidation In Ethanol Response: A Candidate Gene Study Using Caenorhabditis Elegans, Harini Pallikarana Tirumala

Theses and Dissertations

Alcohol use disorder (AUD) is the fourth leading cause of preventable death in the United States, and the fifth leading risk factor for premature death and disability, globally. There are currently very few treatment options for AUD and there is a need for effective preventive and treatment strategies for this condition. AUD risk has a significant hereditary component, with the contribution of genetic factors being estimated to be about 50%. The Davies-Bettinger laboratory uses C. elegans as a model organism to study the contribution of genetic factors in modulating neuronal responses to ethanol. In this project, we examined the role …

Alpha6 Beta2 Subunit Containing Nicotinic Acetylcholine Receptor Contributions To Abuse-Related Effects Of Nicotine And Alcohol, Alexandra M. Stafford

Theses and Dissertations

Pharmacotherapies for tobacco and alcohol cessation are only modestly successful, so it is important to better understand mechanisms underlying their use and abuse. The overarching goal of this research is to assess a6b2 subunit containing nicotinic acetylcholine receptor (a6b2*nAChR; *denotes possible assembly with other subunits) contributions to abuse-related effects of nicotine and alcohol. In the absence of a6b2*nAChR-selective agonists, a6b2*nAChR gain-of-function (a6L9’S) mice provide a tool for selective activation of a6b2*nAChRs. Using the a6L9’S mice together with nicotine doses sub-threshold for stimulation of native nAChRs, these studies tested the hypothesis that activation of a6b2*nAChRs is sufficient to promote neurochemical and …

Identifying Misconceptions Associated With Inaccurate Survey Reporting In The Combined Use Of Caffeine And Alcohol, Kathryn Polak

Theses and Dissertations

Research on college student use of caffeine combined with alcohol (CAC) and public health concern over such use has been hampered by the absence of psychometrically sound measures of caffeine and CAC use. The present study examined agreement between survey (CAS) and interview (TLFB) methods for collecting data on caffeine, alcohol and CAC use. Participants were N=50 college students randomized to complete CAS followed by TLFB or the reverse. Qualitative follow-up interviews with N=15 participants were used to identify factors contributing to CAS-TLFB discrepancies. Responses varied by method of administration, with largest discrepancy magnitudes found for CAC, followed …

Evaluating A Brief Web-Based Prevention Intervention For Risky Alcohol Use Among College Students, Zoe Neale

Theses and Dissertations

The purpose of this study was to evaluate a brief, web-based alcohol prevention intervention program as a universal approach to addressing the range of alcohol behaviors present on college campuses. The sample of freshman college students recruited from Spit for Science (Dick et al., 2014) included 153 intervention participants, and 151 control participants matched on demographics and baseline alcohol variables. Hierarchical multiple regression, logistic regression, and moderated multiple regression were used to compare intervention and control participants on post-intervention alcohol variables. Treatment predicted lower alcohol use disorder (AUD) symptoms, particularly among baseline drinkers. For non-drinkers, the intervention was associated with …

System Genetic Analysis Of Mechanisms Underlying Excessive Alcohol Consumption, Maren L. Smith

Theses and Dissertations

Increased alcohol consumption over time is one of the characteristic symptoms of Alcohol Use Disorder (AUD). The molecular mechanisms underlying this escalation in intake is still the subject of study. However, the mesocortical and mesolimbic dopamine pathways, and the extended amygdala, because of their involvement in reward and reinforcement are believed to play key roles in these behavioral changes. Multiple gene expression studies have shown that alcohol affects the expression of thousands of genes in the brain. The studies discussed in this document use the systems biology technique of co-expression network analysis to attempt to find

patterns within genome-wide expression …

Examining Genetic And Environmental Influences On Alcohol Use And Externalizing Behaviors In African American Adolescents, Neeru Goyal

Theses and Dissertations

The aim of this thesis is to expand our understanding of the etiology of alcohol misuse and related disorders in African Americans using genetically informative study designs. Specifically, we take advantage of the candidate-gene approach and polygenic score analysis to extend the literature specific to African American populations. Chapter 2 explores gene x environment (GxE) interactions through the candidate gene approach to explore the relationship between two genes chosen on their potential relevance to stress response and adolescent alcohol use and misuse, among African American youth living in highly impoverished neighborhoods, as moderated by stressful life events. Chapter 3 implements …

Alcohol And Medication Use In Community-Dwelling Older Adults: Understanding The Effect Of Alcohol And Central Nervous System-Acting Medications On The Risk For Falls, Maitreyee Mohanty

Theses and Dissertations

Introduction: Aging, comorbid conditions, and use of medications render older adults more susceptible to alcohol-disease or alcohol-drug interactions that may lead to harmful outcomes. In this dissertation project the risk profile of alcohol and medications use among older adults was investigated. Considering the rise in CNS-acting medication use and the adverse effect profile linked to CNS-acting medications, it was also of interest to find if older adults were at risk of falling due to interactions between alcohol and CNS-acting medication. Objectives: The objectives were as follows: 1) to determine the prevalence, pattern and factors associated with at-risk drinking, 2) to …

Behavioral And Genomic Characterization Of Scheduled Ethanol Deprivation, Jonathan Warner

Theses and Dissertations

Alcoholism is a persistent substance abuse disorder that is associated with negative health, social, and economic outcomes. Treatment strategies for alcohol use disorders are limited, and only three drugs have been approved by the FDA for treatment. Although behavioral therapy and drug combination strategies improve abstinence outcomes, the majority of those in treatment will not achieve long-term abstinence. Therefore, better treatment strategies are needed. While much progress has been made toward understanding the neurobiology of alcoholism, this knowledge has not been effectively translated into treatment strategies. Animal models of alcohol drinking have been crucial to this research effort, but until …

Genetic Dissection Of Quantitative Trait Loci For Substances Of Abuse, Jo Lynne Harenza

Theses and Dissertations

It has been reported that an individual’s initial level of response to a drug might be predictive of his or her future risk of becoming dependent, thus basal gene expression profiles underlying those drug responses may be informative for both predicting addiction susceptibility and determining targets for intervention. This dissertation research aims to elucidate genetic risk factors underlying acute alcohol and nicotine dependence phenotypes using mouse genetic models of addiction. Phenotyping, brain region-specific mRNA expression profiling, and genetic mapping of a recombinant inbred panel of over 25 mouse strains were performed in order to identify quantitative trait loci (QTL) harboring …

The Effects Of Sleep Problems And Depression On Alcohol-Related Negative Consequences Among College Students, Mcgann Amanda Wattenmaker

Theses and Dissertations

Previous literature provides an overview of the multiple relationships between alcohol use, protective behavioral strategies (PBS), alcohol-related negative consequences, depression, and sleep problems among college students, as well as differences by individual level characteristics, such as age, gender, and race/ethnicity. Several studies have found that specific demographic groups of students are more likely to reach a higher blood alcohol content (BAC) when “partying” or socializing (Turner, Bauerle, & Shu, 2004; Crotty, 2011). A variety of studies have also confirmed the positive relationship between high blood alcohol content and experiencing alcohol-related negative consequences (Turner, et al., 2004; Martens, Taylor, Damann, Page, …

Reducing Recidivism In Returning Offenders With Alcohol And Drug Related Offenses: Contracts For The Delivery Of Authentic Peer Based Recovery Support Services, Sarah Scarbrough

Theses and Dissertations

In collaboration with Sheriff C. T. Woody, the Deputies and other jail personnel, Kingdom Life Ministries (KLM) operates in the City of Richmond Jail. Aimed at serving individuals who suffer from alcoholism and other drug addictions, KLM’s programs offer peer-to-peer recovery support services; meaning people who are successful in their recovery deliver the recovery message. On any given day, rehabilitation and recovery services are provided to 120 men in what used to be the worst tier of the Richmond City Jail. A large portion of these men battle substance abuse disorders and have exhibited habitual criminal behavior over an extended …

Effects Of Early Alcohol Exposure On Ocular Dominance Plasticity, Crystal Lantz

Theses and Dissertations

Fetal alcohol spectrum disorder is the leading cause of mental retardation in the western world. It is associated with learning and sensory deficits. Some of these deficits are a result of faulty neuronal plasticity. Previously our lab has used ferrets to demonstrate that alcohol exposure during the third trimester of human gestation results in impaired ocular dominance plasticity (ODP). Here we have transferred this model to mice. Mice, treated with 5 mg/kg of ethanol on postnatal days 5, 7 and 9, exhibit a lack of ODP plasticity after 10 days of monocular deprivation (MD) during the critical period of visual …

Identifying Modulators Of The Development Of Acute Functional Tolerance To Ethanol In Caenorhabditis Elegans., Ka-Po Leung

Theses and Dissertations

Alcohol abuse is a problem in our society. There are few treatments available, in part due to the unclear molecular mechanisms of ethanol’s effects. Human studies indicate that there is a genetic component influencing disease susceptibility, and that an individual’s initial response to alcohol can predict their development of addiction. We have taken a forward genetics approach to study one component of initial response, acute functional tolerance (AFT), in Caenorhabditis elegans. We identified bet11, a mutation that causes animals to be defective in the development of AFT. Genetic analysis suggested that the gene that bet11 disrupts participates in a synthetic …

Parent-Child Communication About Substance Use: Experiences Of Latino Emerging Adults, Kathryn Reid-Quiñones

Theses and Dissertations

The purpose of the current studies was to identify messages that Latino parents communicate to their offspring about the use of legal and illegal drugs and to determine associations between parental messages and substance use outcomes. Previous research has identified parent-child communication as protective against tobacco, alcohol, and other drug use. However, most of these studies have failed to examine the specific messages communicated and those that have focused almost exclusively on non-Hispanic Caucasians. Study 1 identified messages that Latino parents communicate to their offspring regarding legal and illegal drugs through two focus groups with Latino college students (N = …

Forgiveness And The Bottle: Promoting Self-Forgiveness With Alcohol Misuse, Michael Scherer

Theses and Dissertations

Forgiveness research has seldom been directed toward alcohol use, misuse, and abuse. To date, forgiveness research in the realm of alcohol use, misuse, and abuse has focused on interpersonal consequences (e.g., Lin, Mack, Enright, Krahn, & Baskin, 2005; Scherer, Worthington, Hook, Campana, West, & Gartner, 2009; Worthington, Scherer, & Cooke, 2006), but has paid minimal attention to intrapersonal consequences. Psychologists today are just beginning to explore the complex and murky waters of self-forgiveness (or lack of it) and the alcohol misuser (e.g., Webb, Robinson, Brower, & Zucker, 2006). In the current dissertation, I review the literature on self-forgiveness, drinking refusal …

Differences In Peer Perception Of Alcohol Use, Personal Alcohol Use, And Levels Of Intoxication Among Students At Virginia Commonwealth University From 2002 To 2004., William Evans

Theses and Dissertations

This study involves the examination of National Collegiate Health Assessment (NCHA) data collected by the VCU Wellness Resource Center. This study will compare trends in college student health behavior perceptions and personal activity regarding alcohol use, as self-reported via the NCHA data, with a particular focus on a comparison between 2002, which is the year that the Wellness Resource Center (then known as the Office of Health Promotion) first implemented an alcohol education campaign based upon a “social norms” theoretical framework, and 2004, after 18 months of intensive campaigning. Thus, the aim of the project is to examine the changes …

Behavioral And Molecular Analysis Of Individual Variation In Ethanol Drinking, Jennifer Wolstenholme

Theses and Dissertations

A majority of Americans regularly consume alcohol, but the risk factors leading to excessive drinking and alcohol abuse are unevenly distributed throughout the population. Genetic differences can account for only 40-60% of this variability. While variations in ethanol preference drinking in rodent models have been reported, the neurobiological factors underlying these behaviors are still not completely understood. Thus, these studies were designed to determine behavioral and molecular factors associated with the initiation of ethanol drinking preference in an inbred mouse model. We harnessed the power of inter-individual variation of ethanol drinking within an inbred mouse strain to essentially eliminate genetic …

Religiosity, Parental Support And Adult Support Coping As Protective Factors For Drug Refusal Efficacy And Use Among African American Adolescents, Raymond H. Tademy

Theses and Dissertations

This study examined whether religiosity, parental and adult support coping would moderate the influence of neighborhood risks and friends' drug use upon drug refusal efficacy and drug use among African American adolescents. One hundred and thirteen African American urban adolescents (77 females and 36 males) aged 11-17 (M=14.17) participated in this study. This study used the God Support and Religious Support scales to assess religiosity; the parental support coping subscale of the Wills Coping measure; Center for Substance Abuse Prevention's Special Event Drug Refusal Efficacy and Friends' Drug use scales; the Exposure to Neighborhood Risk Scale; and a one-item measure …

The Motives And Experiences Of College Students Who Choose To Abstain From Drinking Alcohol, Lisa Proakis-Stone

Theses and Dissertations

Objective: Numerous studies trying to find the causes and implications of binge drinking on college campuses have focused their attention on the heavy drinkers. The purpose of this study was to understand why and how college students choose to abstain from drinking. The study also examined the experiences of the abstaining college students on a campus where 83% of the student body drinks. Methods: Twelve undergraduate students from the University of Richmond participated in this qualitative study. Individual interviews using open-ended questions were conducted to ascertain the reasons for their abstinence and their experiences as college students. After the interviews, …

Panic Disorder, Trait Anxiety, And Risk Drinking In Pregnant And Non-Pregnant Women, Sarah Meshberg-Cohen

Theses and Dissertations

Anxiety disorders, including Panic Disorder, and alcohol problems co-occur at greater rates than chance in the general population. It has also been suggested that alcohol is used to cope with anxiety symptoms, such as trait anxiety. While pregnancy may be a protective period against Panic Disorder and panic symptoms, trait anxiety remains relatively stable during pregnancy. The purpose of the present study was to examine differences in rates of current Panic Disorder, panic attacks, and trait anxiety in pregnant and non-pregnant women receiving care at an urban OB/GYN clinic. The study also examined correlates and differences in alcohol use and …

Recovery From Substance Abuse: The Role Of Unsupportive Social Interactions, Michelle Marie Schmitt

Theses and Dissertations

ABSTRACTRECOVERY FROM SUBSTANCE ABUSE: THE ROLE OF UNSUPPORTIVE SOCIAL INTERATIONSBy Michelle M. Schmitt, M.A., M.S. A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University.Virginia Commonwealth University, 2003Major Director: Kathleen M. Ingram, Ph.D., Assistant Professor, Department of Psychology It has been estimated that over 20 million individuals struggle with substance abuse and/or dependence each year. Theories of addiction and recovery have long incorporated the notion of social support and helping relationships to facilitate adjustment. However, the literature investigating the role of social support is fraught with contradictions. In addition, in …