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Spouse Violence In Case Of Police Officers In Puerto Rico: Violence In Police Families, Jorge Ismael Suarez

Theses and Dissertations

This applied dissertation was designed to explore the relationship between factors related to police work and whether these factors help predict domestic violence in a sample of officers of the Puerto Rico Police. The following factors were explored in this study: external burnout, alcohol abuse, department withdrawal, and authoritarian spillover. This study was based on a previous study by Johnson et al. (2005), which revealed that violence exposure and domestic violence among police officers are linked according to four mediation chains. The mediation chain was a model implemented by the authors to determine the influence of burnout, authoritarian spillover, alcohol …

Catharanthine Modulates Mesolimbic Dopamine Transmission: A Potential Treatment For Alcohol Use Disorder, Benjamin M. Williams

Theses and Dissertations

Catharanthine is derived from the Catharanthus roseus plant and is an analog to ibogaine, a drug that reduces opioid and alcohol withdrawal symptoms and decreases drug self-administration in both animals and humans. Catharanthine has promise to be an alternative pharmacological treatment for addiction without the adverse side effects associated with ibogaine. The objective of this study was to evaluate catharanthine’s effects on dopamine (DA) transmission in the mesolimbic DA system as well as determine its effects on both ethanol withdrawal induced anxiety and drug-seeking behaviors in mice. We hypothesized that catharanthine would inhibit evoked DA release in the nucleus accumbens …

Perfectionism, Alcohol Intoxication, And Deliberate Self-Harm In Men And Women, Lissa N. Mandell

Theses and Dissertations

Previous research has demonstrated associations between deliberate self-harm (DSH) and perfectionism, although most of that research used retrospective self-report measures of DSH, which are prone to various cognitive biases. Although perfectionism has been associated with alcohol abuse, no research has examined how alcohol intoxication may moderate the relation between perfectionism and DSH. The aims of this experimental study were to determine if perfectionism is associated with a laboratory analogue of DSH (the Self-Aggression Paradigm) and examine the role of alcohol intoxication as a potential moderator. Using archival data, blood alcohol content (BAC) was manipulated by randomly assigning participants to reach …

The Relation Between Adhd Symptoms And Alcohol And Marijuana Use Outcomes Among College Students: The Mediating Role Of Experiential Avoidance, William Joshua Bradley

Theses and Dissertations

Individuals with attention-deficit/hyperactivity disorder (ADHD) are at significantly greater risk for alcohol and marijuana misuse and abuse compared to their peers. College students with ADHD may be particularly vulnerable to problematic alcohol and marijuana use, given the widespread use and abuse of these substances by college students generally and liability associated with ADHD specifically. Experiential avoidance, or avoidance of unwanted thoughts, feelings, and other internal experiences, is positively associated with ADHD symptoms and substance use problems. However, it is unclear what role experiential avoidance plays in the relation between ADHD and alcohol and marijuana use. This study examined whether experiential …

Evaluation Of Bmi, Diet Quality, Alcohol Intake, And Physical Activity In Cancer Survivors: Is There An Association With Primary Source Of Nutrition Advice?, Zachary J. Heindl

Theses and Dissertations

Objective: The purpose of this study was to determine if cancer survivors are meeting nutrition and physical activity recommendations presented by the ACS. The association between a survivor’s primary source of nutrition advice and these factors was also assessed. Method: A sample of 132 cancer survivors was recruited through social media. Inclusion criteria included: United States citizenship, at least 18 years of age, and diagnosis of cancer or malignancy by a health care professional. A one-time, self-administered survey was distributed via Qualtrics. The Dietary Screener Questionnaire was utilized to determine average daily consumption of fruits and vegetables and the weekly …

Alcohol And Other Addictive Behaviors Among Bariatric Surgery Patients, Rachel M. Arnold

Theses and Dissertations

Research has repeatedly highlighted a high rate of comorbidity of addictive behaviors in both clinical and non-clinical samples. While polydrug use has received significant attention in recent years, less is known about rates of comorbidity with behavioral addictions such as eating, work, exercise, or sex. Individuals with a history of bariatric surgery may provide a unique opportunity to examine the potential co-occurrence of addictive behaviors. High rates of food addiction symptoms and changes in alcohol use patterns post-surgically have highlighted a potential gap in our understanding of bariatric patients and their needs post-surgically. Using a composite measure of addictive behaviors, …

Direct And Indirect Effects Of Alcohol Craving And Demand On Obsessive And Compulsive Drinking Characteristics: The Role Of Impulsivity, James V. Turner Iv

Theses and Dissertations

This study’s goal was to test a novel model of obsessive-compulsive thoughts about drinking. Young adults completed tasks measuring potential drinking characteristics. Findings indicated demand was related to higher OCTD, a significant effect of CIC on OCTD via demand, and that the relationship was particularly strong for impulsive individuals.

The Role Of Alcohol Use, Drinking Context, And Alcohol Expectancies In Sexual Assault Perpetration Among College Men, Joseph D. Censor

Theses and Dissertations

Sexual assault is a major public health and criminal justice problem in our society. The high prevalence rate of sexual assault victimization and perpetration among college students is even more disturbing. Additionally, the prevalence of alcohol use among college students is higher than in the general population, and alcohol use is associated with more than half of sexual assaults. The goal of the present study was to gain a deeper understanding of the role of various alcohol related factors in sexual assault perpetration among college men. Specifically, this study assessed the association of perpetration with general problematic alcohol use, general …

Genetics And Alcohol Interventions In Youth, Zoe E. Neale

Theses and Dissertations

Alcohol is the most commonly used substance among youth, and risky alcohol use is associated with harmful consequences such as accidents, academic consequences, and physical and emotional health problems. Alcohol use disorders are approximately 50% heritable, yet most efforts to prevent and intervene upon youth alcohol use focus only on environmental factors. Furthermore, current prevention and intervention programs tend to have modest effects and are not uniformly effective for all individuals. Gene-by-intervention (GxI) studies offer an opportunity to expand current understanding of interventions by examining whether underlying genetic risk may contribute to differential program effects. Much of the current GxI …

Identifying Genes Downstream Of Mef2 That Influence Ethanol Sedation In Drosophila Melanogaster, Ananya Talikoti

Theses and Dissertations

Alcohol use disorder is a global public health issue that affects millions across the world. It can result in negative physical and mental health outcomes, and currently treatment options are limited and rates of relapse are high. Identifying genes that affect aspects of ethanol behaviors in model organisms, such as Drosophila melanogaster, can serve to eventually develop more robust therapeutic interventions for those experiencing alcohol dependence. Previous studies have identified a relationship between a person's initial sensitivity to alcohol and their abuse potential for the drug in later life. Therefore, we can study sedation behaviors in Drosophila melanogaster to …

The Effects Of Alcohol On Bdnf And Cd5 Dependent Pathways, Andrew Jordan Payne

Theses and Dissertations

Alcohol represents the third leading cause of preventable death in the United States. Yet, despite its prevalent role in impeding human health, there is much to understand about how it elicits its effects on the body and how the body and brain change when an individual becomes physiologically dependent upon alcohol. The work presented herein represents an effort to elucidate the acute and chronic effects of alcohol on the nervous system. We investigate two specific protein pathways and their role in alcohol's effects on the body. The first begins with brain-derived neurotrophic factor (BDNF), which acts on TrkB, and ends …

Alcohol Affects The Reward Pathway Of The Brain Via A6-Containing Nicotinic Acetylcholine Receptors In The Nucleus Accumbens, Elizabeth Qiufeng Anderson

Theses and Dissertations

The prevailing view is that enhancement of dopamine (DA) transmission in the mesolimbic system consisting of DA neurons in the ventral tegmental area (VTA) that project to the nucleus accumbens (NAc) underlies the rewarding properties of ethanol (EtOH) and nicotine (NIC). Although the dogma is that EtOH enhancement of DA neural activity contributes to enhancement of DA transmission, DA neurons are not sensitive to rewarding levels of EtOH. However, VTA GABA neurons are sensitive to low-dose EtOH. We have shown previously that EtOH modulation of DA release in the NAc is mediated by α6-containing nicotinic receptors (α6*-nAChRs), that α6*-nAChRs mediate …

Aerobic Exercise Alters Opioid Receptors Following Chronic Alcohol Exposure, James N. Brundage

Theses and Dissertations

Opioid receptors have been a target of pharmacological manipulation in alcohol use disorder (AUD) recovery protocols for many years. Aerobic exercise, a common adjunct in AUD recovery, is known to modulate opioid receptors (ORs) both during both acute and long term exposure. The three subtypes of ORs: mu (MOR), delta (DOR), and kappa (KOR) are all expressed on neurons in the mesocorticolimbic circuitry. Kappa-opioid receptors are expressed directly on dopamine (DA) neuron terminals in the nucleus accumbens (NAc). Mu and Delta ORs are expressed on cholinergic interneurons (CINs) and GABA neurons in the NAc. In alcohol dependent rodents, KORs are …

Choline Supplementation And Dna Methylation In The Hippocampus And Prefrontal Cortex Of Rats Exposed To Alcohol During Development, Nicha K. H. Otero

Theses and Dissertations

Fetal Alcohol Spectrum Disorders (FASD) refers to the effects of alcohol exposure during development on individuals. Some of the most frequent deficits seen in children with FASD and in animal models of FASD are spatial memory impairments and impaired executive functioning, which are likely related to alcohol-induced alterations of the hippocampus and medial prefrontal cortex (mPFC), respectively. Choline, a nutrient supplement, has been shown in a rat model to ameliorate some of alcohol‟s teratogenic effects and this effect may be mediated through choline‟ effects on DNA methylation. This study used a rat model of FASD to investigate the underlying mechanisms …

The Effect Of Hurricane Sandy On Mental Health Outcomes Of New Yorkers, Kacper Perkowski

Theses and Dissertations

This study’s objective is to evaluate the effect of Hurricane Sandy on the long-term mental health of New Yorkers. Health data spanning from 2010-2014 was obtained through the Community Health Survey. Difference-in-differences regressions were employed to evaluate the relationship. The results show Hurricane Sandy had no statistically significant effect on any key mental health variables.

The Interaction Between Caffeine Consumption, Alcohol Use, And Amount Of Sleep On Bone Health, Haley Davis-Martin

Theses and Dissertations

Using cross-sectional data from the 2009-2010 and 2013-2014 NHANES surveys we examined potential interaction between caffeine consumption, alcohol use, and sleep and its relation to bone health, measured using bone mineral density (BMD). The study population consisted of 2,405 individuals at least 40 years old who were not taking any medications or hormones that would affect bone mineral density. Several factors such as smoking status, exercise, dietary intake of calcium, dietary intake of vitamin D, dietary intake of phosphorus, presence of arthritis, age, gender and race were considered in this project. We found a statistically significant negative linear relationships between …

Attitudes About Date Rape: Context And Consent, Hannah Mae Strom

Theses and Dissertations

Sexual assault, and date rape specifically, is a prevalent problem among college students. Many factors influence students’ attitudes about these situations such as the closeness of the relationship and alcohol consumed at the time of the incident. These same factors similarly influence attitudes about reporting an assault. The development of mobile proximity-based dating applications (dating apps) has added a new, and largely under-researched, factor of influence to attitudes about perceptions of sexual consent. The current study used vignettes to examine college students’ perceptions of sexual consent and attitudes about reporting in a date rape scenario. The vignettes varied to explore …

Mef2-Bound Genes May Influence Ethanol Sedation In Drosophila Melanogaster, Katlyn M. Myers

Theses and Dissertations

Alcohol Use Disorder (AUD) is a global health issue that affects millions of people every year. This disorder has serious negative mental and physical consequences. Currently, treatment options for this disorder are largely limited to psychological therapy, with very few medications available to treat it. Being able to identify the environmental and genetic components that influence AUD can help improve diagnosis and treatment options. Previous studies in humans have shown a link between initial sensitivity and risk for alcohol abuse. Our laboratory uses Drosophila melanogaster as a model to study the genetic and environmental components of alcohol-related behaviors. Previous lab …

Transcriptomic Profiling Of Postmortem Prefrontal Cortex And Nucleus Accumbens From Chronic Alcohol Abusers., Eric S. Vornholt

Theses and Dissertations

Alcohol use disorder (AUD) is a debilitating psychiatric illness that develops from a combination of genetic and environmental factors. While it is well documented that AUD is heritable, the shift from recreational alcohol use to abuse/dependence is poorly understood. In this dissertation, using postmortem brain tissue from individuals with alcohol dependence (AD), we profiled the genome-wide expression of circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) to better understand the impact of gene expression on the development of AUD. To achieve this, we performed two independent studies that explore transcriptome differences between AD cases and controls. The first of …

An Examination Of Impaired Driving: The Integral Role Of Cognitive And Behavioral Predictors, Andrew Tatch

Theses and Dissertations

Despite modest reductions over previous decades, improvements in impaired driving prevalence have stalled at problematic levels in recent years. Recent self-report data indicate that 20 percent of driving age individuals acknowledge operating a vehicle within two hours of alcohol consumption within the previous year and there are approximately 121 million episodes of impaired driving annually. Extant research has consistently identified specific subgroups, including men, young adults, and individuals with less education, as being high risk for driving under the influence. Additionally, researchers of impaired driving have discerned certain impaired driving-related attitudes and behaviors as important predictors of impaired driving. Despite …

Mechanoreceptor Activation In The Treatment Of Drug-Use Disorders: Mechanism And Outcome, Kyle Bills

Theses and Dissertations

The therapeutic benefits attributed to activation of peripheral mechanoreceptors are poorly understood. There is growing evidence that mechanical stimulation modulates substrates in the supraspinal central nervous system (CNS) that are outside the canonical somatosensory circuits. This work demonstrates that activation of peripheral mechnoreceptors via mechanical stimulation (MStim) is sufficient to increase dopamine release in the nucleus accumbens (NAc), alter neuron firing rate in the ventral tegmental area (VTA) and increase membrane translocation of delta opioid receptors (DORs) in the NAc. Further, we demonstrate that these effects are dependent on DORs and acetylcholine receptors. Additionally, MStim can block neuronal markers of …

Tuning Size And Light Emission Characteristics Of Cdses/Zns Alloyed Core-Shell Quantum Dots By Alcohol, John Casey Marnocha

Theses and Dissertations

In this thesis, a one-step isotropic down-tuning of the size of alloyed CdSe1−xSx/ZnS core- shell quantum dots is developed by vapor and liquid-phase etching using a ∼ 10 : 1 isopropyl alcohol to water solution. The results of spectroscopic studies confirm continuous variation of the excitonic emission color from red to green for samples treated for up to 15 mins using a vapor-based method. According to HRTEM characterizations, the shift is consistent with a diameter reduction of quantum dots that proceeds at the average rate of ∼ 1.3 ̊A/min. The emission intensity while seen to undergo an initial drop due …

Tuning Size And Light Emission Characteristics Of Cdses/Zns Alloyed Core-Shell Quantum Dots By Alcohol, John Casey Marnocha

Theses and Dissertations

In this thesis, a one-step isotropic down-tuning of the size of alloyed CdSe1−xSx/ZnS core- shell quantum dots is developed by vapor and liquid-phase etching using a ∼ 10 : 1 isopropyl alcohol to water solution. The results of spectroscopic studies confirm continuous variation of the excitonic emission color from red to green for samples treated for up to 15 mins using a vapor-based method. According to HRTEM characterizations, the shift is consistent with a diameter reduction of quantum dots that proceeds at the average rate of ∼ 1.3 ̊A/min. The emission intensity while seen to undergo an initial drop due …

Glial Cell Mechanisms Regulate Alcohol Sedation In Drosophila Melanogaster, Kristen M. Lee

Theses and Dissertations

Approximately 16 million people in America are diagnosed with Alcohol Use Disorder (AUD) but no efficacious medical treatments exist. Alcohol-related behaviors can be studied in model organisms, and changes in these behaviors can be correlated with either (i) a risk for alcohol dependence or (ii) a symptom/feature of AUD itself. Although AUD is a disease of the central nervous system, a majority of research has focused on the neuronal underpinnings, leaving glial contributions largely undescribed. We used Drosophila melanogaster (fruit fly) to identify genes whose expression in glia regulates alcohol sedation. Mammals and Drosophila have conserved behavioral responses to alcohol …

Genome-Wide Systems Genetics Of Alcohol Consumption And Dependence, Kristin Mignogna

Theses and Dissertations

Widely effective treatment for alcohol use disorder is not yet available, because the exact biological mechanisms that underlie this disorder are not completely understood. One way to gain a better understanding of these mechanisms is to examine the genetic frameworks that contribute to the risk for developing this disorder. This dissertation examines genetic association data in combination with gene expression networks in the brain to identify functional groups of genes associated with alcohol consumption and dependence.

The first study took advantage of the behavioral complexity of human samples, and experimental capabilities provided by mouse models, by co-analyzing gene expression networks …

Cellular Mechanisms By Which Alcohol Promotes Hiv Protease Inhibitor-Induced Hepatotoxicity, Michael Hinton

Theses and Dissertations

CELLULAR MECHANISMS BY WHICH ALCOHOL PROMOTES HIV PROTEASE INHIBITOR-INDUCED HEPATOTOXICITY

Michael Hinton, B.S.

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University

Virginia Commonwealth University, 2019

Major Director: Huiping Zhou

Professor, Department of Microbiology and Immunology

The development of highly-active-antiretroviral therapy(HAART) has allowed management of HIV and extended the lives of those infected. Alcohol abuse, which is very common in HIV-1 infected patients, is one of the most important co-morbid risk factors for liver injury and has been associated with the occurrence of serious metabolic syndrome and subsequent discontinuation …

Baked And Buzzed: Investigating The Influence Of Co-Use Of Cannabis And Alcohol On White Matter Integrity In Emerging Adults, Natasha E. Wright

Theses and Dissertations

Objective: Growing evidence suggests alcohol and cannabis use independently alter neural structure and functioning, particularly during sensitive developmental time periods such as adolescence and emerging adulthood. However, there has been minimal investigation into the effects co-occurring use of these two substances, despite preliminary evidence of unique acute and psychopharmacological changes due to using alcohol and cannabis together.

Method: Data drawn from the IDEAA Consortium was utilized to assess white matter integrity as measured by FreeSurfer’s TRACULA in emerging adults (n=192; 16-27 years old). Timeline Follow-Back was used to calculate past month cannabis use, alcohol use, co-use days, binge alcohol episode, …

Neuroimmune-Mediated Alcohol Effects On Ventral Tegmental Area Microglia And Infiltrating Leukocytes, Travis Jonathan Clarke

Theses and Dissertations

Microglia are the primary immune cell in the central nervous system and are known as the “resident” macrophages in the central nervous system (CNS). While microglia are classically known as the immune cells of the CNS, their role has more recently been shown to extend far beyond immunity. The effects of ethanol on the brain are closely linked to neuroimmune responses mediated by microglia that are present in the healthy brain from the time of development. Though microglia have been classified as the “resident” immune cells of the CNS, new research suggests that other immune cells may be implicated in …

The Effects Of Instructors Discussing Alcohol In The Classroom On Student Perceptions Of Their Instructor, Nathaniel Swiech Brophy

Theses and Dissertations

This thesis is comprised of two studies. Study I is a qualitative exploratory analysis which attempts to uncover students’ experiences with instructors discussing alcohol in the university classroom and how the appropriateness of this behavior is determined. How this mention of alcohol affects the students’ perception of the instructor, as well as the relationship between perceived appropriateness of the behavior and change in perception, are also investigated. Study I found that students have experienced their instructors mentioning alcohol in the classroom as normative student behavior, part of the formal curriculum, and their instructor disclosing personal alcohol use. Participants determined appropriateness …

Neuroimmune-Mediated Alcohol Effects On Ventral Tegmental Area Neurons, Stephanie Bair Williams

Theses and Dissertations

Dopamine (DA) transmission is a key player in the rewarding aspects of ethanol as well as ethanol dependence. The current dogma is that DA transmission is increased during ethanol via the inhibition of ventral tegmental area (VTA) GABA neurons and that excitation of VTA GABA neurons during withdrawal results in decreased DA transmission. Microglia, the major neuroimmune effector in the brain, may be a key mediator in this process by releasing cytokines following activation. We evaluated the effect of ethanol on cytokine concentrations in the VTA and NAc using a cytometric bead array, and found that low dose ethanol (1.0 …