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Abundance And Diversity Of Ammonia-Oxidizing Archaea And Bacteria In Sediments Of Trophic End Members Of The Laurentian Great Lakes, Erie And Superior, Annette Bollmann, George S. Bullerjahn, Robert Michael Mckay May 2014

Abundance And Diversity Of Ammonia-Oxidizing Archaea And Bacteria In Sediments Of Trophic End Members Of The Laurentian Great Lakes, Erie And Superior, Annette Bollmann, George S. Bullerjahn, Robert Michael Mckay

Great Lakes Institute for Environmental Research Publications

Ammonia oxidation is the first step of nitrification carried out by ammonia-oxidizing Archaea (AOA) and Bacteria (AOB). Lake Superior and Erie are part of the Great Lakes system differing in trophic status with Lake Superior being oligotrophic and Lake Erie meso- to eutrophic. Sediment samples were collected from both lakes and used to characterize abundance and diversity of AOA and AOB based on the ammonia monooxygenase (amoA) gene. Diversity was accessed by a pyro-sequencing approach and the obtained sequences were used to determine the phylogeny and alpha and beta diversity of the AOA and AOB populations. In Lake Erie copy …


Developing Molecular Tools To Assess The Biogeochemical/Microbial Community Structure Of Oil Sand Processed Waste Material, Sabari Prakasan Mullapulli Raveendran Jan 2014

Developing Molecular Tools To Assess The Biogeochemical/Microbial Community Structure Of Oil Sand Processed Waste Material, Sabari Prakasan Mullapulli Raveendran

Electronic Theses and Dissertations

Microbial communities can dominate Fluid Fine Tailings (FFT) in the presence of electron acceptors (e.g. Sulfate). Sulfate reduction can produce hydrogen sulfide, one of several chemical constituents responsible for sediment oxygen demand (SOD). The preservation of RNA is a crucial step to study active microbial populations and their activity in FFT and hence understand the biological factors contributing to SOD. In our study different RNA preservation methods were tested to preserve microbial RNA in FFT sample. The results confirmed that LifeGuard(TM) Soil Preservation Solution (MO BIO Laboratories, Inc, California) is the best preservative method for RNA preservation. Through T-RFLP analysis …


Individual-Based Modeling And Nonlinear Analysis For Complex Systems With Application To Theoretical Ecology, Abbas Ghadri Golestani Jan 2014

Individual-Based Modeling And Nonlinear Analysis For Complex Systems With Application To Theoretical Ecology, Abbas Ghadri Golestani

Electronic Theses and Dissertations

One approach to understanding the behaviour of complex systems is individual-based modeling, which provides a bottom-up approach allowing for the consideration of the traits and behaviour of individual organisms. Ecosystem models aim to characterize the major dynamics of ecosystems, in order to synthesize the understanding of such systems and to allow predictions of their behaviour. Moreover, ecosystem simulations have the potential to help scientists address theoretical questions as well as helping with ecological resource management. Because in reality biologists do not have much data regarding variations in ecosystems over long periods of time, using the results of ecological computer simulation …


Spatial And Temporal Variability In The Carrion Insect Community: Using Blow Flies (Family: Calliphoridae) As A Model System To Study Coexistence Mechanisms At Multiple Scales, Jennifer Yvonne Rosati Jan 2014

Spatial And Temporal Variability In The Carrion Insect Community: Using Blow Flies (Family: Calliphoridae) As A Model System To Study Coexistence Mechanisms At Multiple Scales, Jennifer Yvonne Rosati

Electronic Theses and Dissertations

Resource partitioning can lead to species coexistence. In a field study, temporal and spatial partitioning were examined by testing the effects of season and habitat on the structure of the blow fly community on domestic pig carcasses, Sus scrofa domesticus in southwestern Ontario, Canada. Blow fly communities did not differ between field and forest habitats, however there were seasonal differences. Fall was characterized by having more species and higher levels of species evenness, diversity, and niche overlap than spring and summer. On a finer scale, effects of arrival order were examined in laboratory experiments with three blow fly species: Phormia …


Genomic And Behavioural Evolution In The Artificial Ecosystem Simulation Ecosim, Marwa Fouad Khater Jan 2014

Genomic And Behavioural Evolution In The Artificial Ecosystem Simulation Ecosim, Marwa Fouad Khater

Electronic Theses and Dissertations

Artificial life evolutionary systems facilitate addressing lots of fundamental questions in evolutionary genetics. Behavioral adaptation requires long term evolution with continuous emergence of new traits, governed by natural selection. We model organism's genomes coding for their behavioral model and represented by fuzzy cognitive maps (FCM), in an individual-based evolutionary ecosystem simulation (EcoSim). The emergent of new traits (genes) in EcoSim is examined by studying their effect on individual's fitness and well being. We examine how the new traits are used to predict the value of fitness using machine learning techniques. A comparison between the genomic evolution of EcoSim and a …