Digital Commons Network^™

The Unseen World: Environmental Microbial Sequencing And Identification Methods For Ecologists, Naupaka Zimmerman, Jacques Izard, Christian Klatt, Jizhong Zhou, Emma Aronson

Department of Food Science and Technology: Faculty Publications

Archaea, bacteria, microeukaryotes, and the viruses that infect them (collectively “microorganisms”) are foundational components of all ecosystems, inhabiting almost every imaginable environment and comprising the majority of the planet’s organismal and evolutionary diversity. Microorganisms play integral roles in ecosystem functioning; are important in the biogeochemical cycling of carbon (C), nitrogen (N), sulfur (S), phosphorus (P), and various metals (eg Barnard et al. 2005); and may be vital to ecosystem responses to large-scale climatic change. Rarely found alone, microorganisms often form complex communities that are dynamic in space and time. For these and other reasons, ecologists and environmental scientists have become …

Breeding Time In A Migratory Songbird Is Predicted By Drought Severity And Group Size, Charles R. Brown, Mary Bomberger Brown

School of Natural Resources: Faculty Publications

Global climate change is altering the breeding phenology of many organisms, and one reported consequence of warmer average temperatures is earlier breeding times in migratory songbirds of north temperate latitudes. Less studied are the potential interactions between earlier breeding and social behavior in colonial species. We investigated how breeding time, as measured by colony initiation dates across the entire summer, in Cliff Swallows (Petrochelidon pyrrhonota) of southwestern Nebraska, USA, changed over a 30-year period and could be predicted by climatic variables, year, and colony size. Mean colony initiation date became earlier over the study, with variation best predicted …

Sympatric Prey Responses To Lethal Top-Predator Control: Predator Manipulation Experiments, Benjamin L. Allen, Lee R. Allen, Richard M. Engeman, Luke K.-P. Leung

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction: Many prey species around the world are suffering declines due to a variety of interacting causes such as land use change, climate change, invasive species and novel disease. Recent studies on the ecological roles of top-predators have suggested that lethal top-predator control by humans (typically undertaken to protect livestock or managed game from predation) is an indirect additional cause of prey declines through trophic cascade effects. Such studies have prompted calls to prohibit lethal top-predator control with the expectation that doing so will result in widespread benefits for biodiversity at all trophic levels. However, applied experiments investigating in situ …

Mate Replacement And Alloparental Care In Ferruginous Hawk (Buteo Regalis), Shubham Datta, Will M. Inselman, Jonathan A. Jenks, Kent C. Jensen, Christopher C. Swanson, Robert W. Klaver, Indrani Sasmal, Troy W. Grovenburg

The Prairie Naturalist

Alloparental care (i.e., care for unrelated offspring) has been documented in various avian species (Maxson 1978, Smith et al. 1996, Tella et al. 1997, Lislevand et al. 2001, Literak and Mraz 2011). A male replacement mate that encounters existing broods has options, which include alloparental care or infanticide. Infanticide may be beneficial in some species (Rohwer 1986, Kermott et al. 1990), but in long-lived avian species, like the ferruginous hawk (Buteo regalis) that do not renest within a season, infanticide might be detrimental. Adoption and rearing success likely provide direct evidence of competence of replacement mates as potential parents for …

Diurnal Raptors Of Buffalo County, Nebraska, Thomas L. Freeman

The Prairie Naturalist

Diurnal birds of prey are apex predators, which often are utilized as indicators of potential environmental change (Newton 1979, Rodriguez-Estrella et al. 1998). While the Platte River Valley of Nebraska and the central plains are recognized as important winter habitat for numer- ous bird of prey species (United States Fish and Wildlife Service [USFWS] 1981, Root 1988), there are few published studies describing the distribution and abundance of raptors in Nebraska (Mathisen and Mathisen 1968, Craighead and Craighead 1969, USFWS 1981). In addition, studies summarizing the long-term distribution or density of Falconi- formes in Nebraska are limited due to the …

Precipitation And Fire Impacts On Small Mammals In Shortgrass Prairie, Whitney J. Priesmeyer, Raymond S. Matlack, Richard T. Kazmair

The Prairie Naturalist

The southern Great Plains and the northern part of the Texas Panhandle have received less attention from a biological perspective than other parts of the state. Although there is substantial information on the effects of fire on small mammals in the tallgrass and mixed-grass prairies, there is a lack of understanding of fire influences in the shortgrass prairie, specifically on small mammals. We conducted our study on the Cross Bar Cooperative Management Area (CMA), a 4,856 ha shortgrass prairie within the Texas panhandle. Our objective was to determine the effect of three different fire return frequencies and precipitation on diversity …

Pore Water Extraction For Unsaturated Zone Isotope Research: An Investigation Using An Immiscible Displacement Fluid And A Centrifuge, Caitlin Burnett Weaver

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Accurate and reliable pore water extraction techniques are important to an array of scientific fields including, but not limited to, hydrogeology, soil science, and paleoenvironmental research. The aim of the current project is to test the applicability of an immiscible displacement extraction technique for stable isotopes of water under a range of textural, hydrologic, and chemical conditions. In this study, laboratory experiments were conducted to establish the extent to which the proposed method 1) achieves sufficient yield for laboratory isotopic analyses, 2) results in isotopic exchange between water and the displacement fluid, 3) conserves initial isotopic compositions of spike test …

Development Modeling Of Lucilia Sericata And Phormia Regina (Diptera: Calliphoridae), Amanda L. Roe

School of Natural Resources: Dissertations, Theses, and Student Research

The relationship between insect development and temperature has been well established and has a wide range of uses, including using blow flies for postmortem (PMI) interval estimations in death investigations. To use insects in estimating PMI, we must be able to determine the insect age at the time of discovery and backtrack to time of oviposition. Unfortunately, existing development models of forensically important insects are only linear approximations and do not take into account the curvilinear properties experienced at extreme temperatures. A series of experiments were conducted with two species of forensically important blow flies (Lucilia sericata and Phormia …

Evolutionary Pressures On Primate Intertemporal Choice, Jeffrey R. Stevens

Department of Psychology: Faculty Publications

From finding food to choosing mates, animals must make intertemporal choices that involve fitness benefits available at different times. Species vary dramatically in their willingness to wait for delayed rewards. Why does this variation across species exist? An adaptive approach to intertemporal choice suggests that time preferences should reflect the temporal problems faced in a species' environment. Here, I use phylogenetic regression to test whether allometric factors (relating to body size), relative brain size, and social group size predict how long 13 primate species will wait in laboratory intertemporal choice tasks. Controlling for phylogeny, a composite allometric factor that includes …

Measuring Soil Frost Depth In Forest Ecosystems With Ground Penetrating Radar, John R. Butnor, John L. Campbell, James B. Shanley, Stanley J. Zarnoch

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Soil frost depth in forest ecosystems can be variable and depends largely on early winter air temperaturesand the amount and timing of snowfall. A thorough evaluation of ecological responses to seasonallyfrozen ground is hampered by our inability to adequately characterize the frequency, depth, durationand intensity of soil frost events. We evaluated the use of ground penetrating radar to nondestructivelydelineate soil frost under field conditions in three forest ecosystems. Soil frost depth was monitoredperiodically using a 900 MHz antenna in South Burlington, Vermont (SB), Sleepers River Watershed,North Danville, Vermont (SR) and Hubbard Brook Experimental Forest, New Hampshire (HBEF) duringwinter 2011–2012 on …

A Global Database Of Soil Nematode Abundance And Functional Group Composition, Johan Hoogen, Peter Mullen, 72 Other Scholars

Department of Plant Pathology: Faculty Publications

As the most abundant animals on earth, nematodes are a dominant component of the soil community. They play critical roles in regulating biogeochemical cycles and vegetation dynamics within and across landscapes and are an indicator of soil biological activity. Here, we present a comprehensive global dataset of soil nematode abundance and functional group composition. This dataset includes 6,825 georeferenced soil samples from all continents and biomes. For geospatial mapping purposes these samples are aggregated into 1,933 unique 1-km pixels, each of which is linked to 73 global environmental covariate data layers. Altogether, this dataset can help to gain insight into …

History Of The University Of Nebraska–Lincoln

2016 Accreditation Materials

The University of Nebraska–Lincoln is a public research university located in Lincoln, Nebraska. It is the state's oldest and largest university and the flagship university of the University of Nebraska system.

Minute Pirate Bug (Orius Insidiosus Say) Populations On Transgenic And Non-Transgenic Maize Using Different Sampling Techniques, Santiago A. Palizada, Bamphitlhi Tiroesele, Difabachew B. Kondidie, Muhammad Irfan Ullah, Fatima Mustafa, Thomas E. Hunt, Pete L. Clark, Jaime Molina-Ochoa, Steven R. Skoda, John E. Foster

Department of Entomology: Faculty Publications

Field experiments were conducted to evaluate the populations of minute pirate bug [Orius insidiosus (Say)] using visual, sticky cards and destructive sampling techniques in transgenic and non-transgenic maize in three locations in Nebraska (Mead, Clay Center. and Concord) United States of America. during 2007 and 2008 . All sampling methods revealed significant counts of O. insidiosus on CP4 EPSPS maize plus an insecticide application for control of first generation O. nubilalis at R2 (blister) sampling period. Similarly, visual observations of O. insidiosus on Cry1Ab x Cry3Bb1 x CP4 EPSPS maize yielded significantly higher mean adult counts at R2 (blister) …

Identification Of A Fourth Haplotype Of Bactericera Cockerelli (Hemiptera: Triozidae) In The United States, Kylie D. Swisher, Donald C. Henne, James M. Crosslin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a pest of potato and other solanaceous crops in North and Central America and New Zealand. Previous genotyping studies have demonstrated the presence of three different haplotypes of B. cockerelli in the United States corresponding to three geographical regions: Central,Western, and Northwestern. These studies utilized psyllids collected in the western and central United States between 1998 and 2011. In an effort to further genotype potato psyllids collected in the 2012 growing season, a fourth B. cockerelli haplotype was discovered corresponding to the Southwestern United States geographical region. High-resolution melting analyses identified …

Water Resources And The Historic Wells Of Barbuda: Tradition, Heritage And Hope For A Sustainable Future, Rebecca Boger, Sophia Perdikaris, Amy E. Potter, John Mussington, Reginald Murphy, Louise Thomas, Calvin Gore, Dwight Finch

Department of Anthropology: Faculty Publications

The island of Barbuda has a relatively unique history, land tenure and geography. Unlike its Caribbean counterparts, the island is not suited to large-scale agriculture due to its arid climate and relatively thin soils. Instead, the enslaved and eventually free people of Barbuda developed a complex herding ecology centered on common land ownership. As a result, carefully designed historic wells are strategically located around the island. With the challenges brought about by climate change, an interdisciplinary, international team led by the Barbuda Research Complex is investigating the state of existing water and food resources and examining how the availability and …

Transdisciplinary Research On Environmental Governance: A View From The Inside, Katherine Mattor, Michele Betsill, Ch'aska Huayhuaca, Heidi Huber-Stearns, Theresa Jedd, Faith Sternlieb, Patrick Bixler, Matthew Luizza, Antony S. Cheng

School of Natural Resources: Faculty Publications

No abstract provided.

Temporal Ecology In The Anthropocene, E. M. Wolkovich, B. I. Cook, K. K. Mclauchlan, T. J. Davies

United States National Aeronautics and Space Administration: Publications

Two fundamental axes – space and time – shape ecological systems. Over the last 30 years spatial ecology has developed as an integrative, multidisciplinary science that has improved our understanding of the ecological consequences of habitat fragmentation and loss. We argue that accelerating climate change – the effective manipulation of time by humans – has generated a current need to build an equivalent framework for temporal ecology. Climate change has at once pressed ecologists to understand and predict ecological dynamics in non-stationary environments, while also challenged fundamental assumptions of many concepts, models and approaches. However, similarities between space and time, …

The Role Of Reserves And Anthropogenic Habitats For Functional Connectivity And Resilience Of Ephemeral Wetlands, Daniel R. Uden, Michelle L. Hellman, David G. Angeler, Craig R. Allen

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Ecological reserves provide important wildlife habitat inmany landscapes, and the functional connectivity of reserves and other suitable habitat patches is crucial for the persistence and resilience of spatially structured populations. To maintain or increase connectivity at spatial scales larger than individual patches, conservation actions may focus on creating andmaintaining reserves and/or influencing management on non-reserves. Using a graph-theoretic approach, we assessed the functional connectivity and spatial distribution of wetlands in the Rainwater Basin of Nebraska, USA, an intensively cultivated agricultural matrix, at four assumed, but ecologically realistic, anuran dispersal distances. We compared connectivity in the current landscape to the historical …

Complexity Versus Certainty In Understanding Species’ Declines, Shana M. Sundstrom, Craig R. Allen

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Aim Our understanding of and ability to predict species declines is limited, despite decades of study. We sought to expand our understanding of species declines within a regional landscape by testing models using both traditional hypotheses and those derived from a complex adaptive systems approach.

Location Our study area was the dry mixed grassland of south-eastern Alberta, Canada, one of the largest remnants of native grassland in North America, and the adjacent grassland in Saskatchewan.

Methods We used the breeding birds of the grassland to test the relationship between species declines and a suite of traits associated with decline (such …

Status Of Native Stream Fishes Within Selected Protected Areas Of The Niobrara River In Western Nebraska, Jonathan J. Spurgeon, Richard H. Stasiak, George R. Cunningham, Kevin L. Pope, Mark A. Pegg

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Lotic systems within the Great Plains are characterized by highly fl uctuating conditions through both space and time. Fishes inhabiting these systems have adopted specifi c life- history strategies to survive in such environments; however, anthropogenic disturbance to prairie streams has resulted in declines and extirpation of many native stream fi shes. Terrestrial protected areas (i.e., parks and reserves) are designated to support native fl ora and fauna and, it is assumed, to provide protection to native fi shes. We assessed the presence and relative abundance of stream fi sh populations within protected areas along the Niobrara River in western …

Overwintering Biology And Tests Of Trap And Relocate As A Conservation Measure For Burying Beetles, W. Wyatt Hoback, Adrienne Conley

Nebraska Department of Transportation: Research Reports

Burying beetles are carrion beetles and utilize dead animal carcasses for feeding and reproductive efforts. They assist with decomposition, prevent the spread of disease, and reduce the number of pest species. The largest species of carrion beetle, the American burying beetle, is a federally endangered insect and its distribution has been reduced by 90%. The conservation of this species is important in maintaining a healthy ecosystem. Overwintering biology and trap and relocation were studied to determine how this beetle survives freezing temperatures and to find whether trap and relocation could be a suitable conservation management measure.

Trap and relocation is …

Characterization Of Management And Environmental Factors Associated With Regional Variations In Potato Zebra Chip Occurrence, F. Workneh, D. C. Henne, J. A. Goolsby, J. M. Crosslin, S. D. Whipple, J. D. Bradshaw, A. Rashed, L. Paetzold, R. M. Harveson, C. M. Rush

Panhandle Research and Extension Center

Potato zebra chip (ZC), caused by the bacterial pathogen ‘Candidatus

Liberibacter solanacearum’, which is vectored by the potato psyllid

(Bactericera cockerelli), has caused widespread damage to U.S. potato

production ever since its first discovery in south Texas in 2000. To

determine the influence of environmental factors and management

practices on ZC occurrence, data on management and meteorological

variables, field locations, and psyllid counts were collected over a 3-year

period (2010 to 2012) from six locations across the central United States

(south Texas to Nebraska). At these locations, ZC-symptomatic plants

were counted in 26 fields from systematically established …

Spring Migration Ecology Of The Mid-Continent Sandhill Crane Population With An Emphasis On Use Of The Central Platte River Valley, Nebraska, Gary L. Krapu, David A. Brandt, Paul J. Kinzel, Aaron T. Pearse

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We conducted a 10-year study (1998–2007) of the Mid-Continent Population (MCP) of sandhill cranes (Grus canadensis) to identify spring-migration corridors, locations of major stopovers, and migration chronology by crane breeding affiliation (western Alaska–Siberia [WA–S], northern Canada–Nunavut [NC–N], west-central Canada–Alaska [WC–A], and east-central Canada–Minnesota [EC–M]). In the Central Platte River Valley (CPRV) of Nebraska, we evaluated factors influencing staging chronology, food habits, fat storage, and habitat use of sandhill cranes. We compared our findings to results from the Platte River Ecology Study conducted during 1978–1980. We determined spring migration corridors used by the breeding affiliations (designated subpopulations for management …

Quantitative Analysis Of Woodpecker Habitat Using High-Resolution Airborne Lidar Estimates Of Forest Structure And Composition, James E. Garabedian, Robert Mcgaughey, Stephen E. Reutebuch, Bernard R. Parresol, John C. Kilgo, Christopher E. Moorman, M. Nils Peterson

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Light detection and ranging (LiDAR) technology has the potential to radically alter theway researchers and managers collect data onwildlife–habitat relationships. To date, the technology has fostered several novel approaches to characterizing avian habitat, but has been limited by the lack of detailed LiDAR-habitat attributes relevant to species across a continuum of spatial grain sizes and habitat requirements. We demonstrate a novel three-step approach for using LiDAR data to evaluate habitat based on multiple habitat attributes and accounting for their influence at multiple grain sizes using federally endangered red-cockaded woodpecker (RCW; Picoides borealis) foraging habitat data fromthe Savannah River Site (SRS) …

Effects Of Winter Recreation On Northern Ungulates With Focus On Moose (Alces Alces) And Snowmobiles, Grant Harris, Ryan M. Nelson, Todd Rinaldi, Thomas Lohuis

United States Fish and Wildlife Service: Publications

Winter recreation can displace ungulates to poor habitats, which may raise their energy expenditure and lower individual survivorship, causing population declines.Winter recreation could be benign, however, if animals habituate. Moreover, recreation creates trails. Traveling on them could reduce energy expenditure, thereby increasing ungulate survivorship and generating population benefits. Balancing recreation use with wildlife stewardship requires identifying when these effects occur. This task would be simpler if guidelines existed to inform assessments. We developed and tested such guidelines using two approaches. First, we synthesized literature describing the effects of winter recreation—motorized and nonmotorized—on northern ungulates. This synthesis enabled formulating six guidelines, …

In Memoriam: Robert Lloyd Rausch—A Life In Nature And Field Biology, 1921–2012, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

During his distinguished career, Robert Rausch, a man of great integrity and principles, served in his lifetime as a singular ambassador for parasitology in a broad international arena. Robert was a friend to many, was honored to be considered an equal among the nomadic bands of Nunamiut traversing the Brooks Range 60 years ago, and will be long remembered for the strength of his science and his perceptive views of the natural world.

Finding Them Before They Find Us: Informatics, Parasites, And Environments In Accelerating Climate Change, Daniel R. Brooks, Eric P. Hoberg, Walter A. Boeger, Scott Lyell Gardner, Kurt E. Galbreath, David Herczeg, Hugo H. Mejía-Madrid, S. Elizabeth Rácz, Altangerel Tsogtsaikhan Dursahinhan

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Parasites are agents of disease in humans, livestock, crops, and wildlife and are powerful representations of the ecological and historical context of the diseases they cause. Recognizing a nexus of professional opportunities and global public need, we gathered at the Cedar Point Biological Station of the University of Nebraska in September 2012 to formulate a cooperative and broad platform for providing essential information about the evolution, ecology, and epidemiology of parasites across host groups, parasite groups, geographical regions, and ecosystem types. A general protocol, documentation–assessment–monitoring–action (DAMA), suggests an integrated proposal to build a proactive capacity to understand, anticipate, and respond …

Suicide Prevention, Deborah M. Stone, Alex E. Crosby

Public Health Resources

Suicide is a public health problem affecting people across the lifespan. It is currently the 10th leading cause of death, with rates having remained relatively flat for the past century. This article summarizes the problem of suicide and suicidal behavior along with suicide prevention efforts in the United States. Part 1 provides an overview of the epidemiology of suicide, including groups most at risk of suicide and suicidal behavior. Part 2 provides a review of common risk factors, organized by developmental life stage. A brief discussion of the lesser well-researched area of protective factors follows. Part 3 provides an overview …

Pentastomids Of Wild Snakes In Australia, Crystal Kelehear, David M. Spratt, Denis O'Meally, Richard Shine

Harold W. Manter Laboratory: Library Materials

Pentastomids are endoparasites of the respiratory system of vertebrates, maturing primarily in carnivorous reptiles. Adult and larval pentastomids can cause severe pathology resulting in the death of their intermediate and definitive hosts. The study of pentastomids is a neglected field, impaired by risk of zoonoses, difficulties in species identification, and life cycle complexities. We surveyed wild snakes in the tropics of Australia to clarify which host species possess these parasites, and then sought to identify these pentastomids using a combination of morphological and molecular techniques. We detected pentastomid infections in 59% of the 81 snakes surveyed. The ubiquity of pentastomid …

Morphologic And Molecular Identifications Of Digenetic Trematodes In Double-Crested Cormorants (Phalacrocorax Auritus) From The Mississippi Delta, Usa, Mary O'Hear, Linda Pote, Marlena Yost, Cynthia Doffitt, D. Tommy King, Carla Panuska

United States Department of Agriculture Wildlife Services: Staff Publications

Increasing numbers of Double-crested Cormorants (Phalacrocorax auritus) in the Mississippi River Delta, USA, have been observed over the past few decades. This piscivorous bird is a definitive host for numerous digenetic trematodes, some of which may cause pathology in a fish host. We conducted a 2-yr survey of intestinal trematodes in 35 Double-crested Cormorants collected in the Mississippi Delta. We counted gastrointestinal trematodes, identified them to species using morphometric and molecular techniques, and sequenced the 18S and cytochrome oxidase I (COI) genes. We collected 4,909 trematodes, representing five digenetic species: Drepanocephalus spathans, Hysteromorpha triloba, Pseudopsilostoma varium, Austrodiplostomum ostrowskiae, and Ascocotyle …