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University of Nebraska - Lincoln

2002

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Loading Microcolumns For The Separation Of Analytes From A Sample In The Millisecond Time Scale, David S. Hage, William A. Clarke Dec 2002

Loading Microcolumns For The Separation Of Analytes From A Sample In The Millisecond Time Scale, David S. Hage, William A. Clarke

David Hage Publications

The present invention generally relates to a microcolumn capable of separating an analyte from a sample in the millisecond time domain. The microcolumn is capable of such rapid separation by employing small column volumes that can tolerate medium to high flow rates. The invention also relates to a method of loading a microcolumn capable of separating an analyte from a sample in the millisecond time domain using plural injections of the packing material.

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Search For Radiative B,-Hadron Decays In Pp̅ Collisions At √S=1.8 Tev, Darin Acosta, Kenneth A. Bloom, Collider Detector At Fermilab Collaboration Dec 2002

Search For Radiative B,-Hadron Decays In Pp̅ Collisions At √S=1.8 Tev, Darin Acosta, Kenneth A. Bloom, Collider Detector At Fermilab Collaboration

Kenneth Bloom Publications

We have performed a search for radiative b-hadron decays using events produced in pp̅ collisions at √s=1.8 TeV and collected by the Collider Detector at Fermilab. The decays we considered were B̅d0→K*0(→K-π+) γ, B̅s0→φ(→K+K-) γ, Λb0→ Λ(→pπ-) γ, and their charge conjugates. Two independent methods to identify photons from such decays were employed. In the first method, the photon was detected in the electromagnetic calorimeter. In the second method, the photon was identified …

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Limits On Extra Dimensions And New Particle Production In The Exclusive Photon, Darin Acosta, Kenneth A. Bloom, Collider Detector At Fermilab Collaboration Dec 2002

Limits On Extra Dimensions And New Particle Production In The Exclusive Photon, Darin Acosta, Kenneth A. Bloom, Collider Detector At Fermilab Collaboration

Kenneth Bloom Publications

The exclusive γE/T signal has a small standard model cross section and is thus a channel sensitive to new physics. This signature is predicted by models with a superlight gravitino or with large extra spatial dimensions. We search for such signals at the Collider Detector at Fermilab, using 87   pb-1 of data at √s=1.8   TeV, and extract 95% C.L. limits on these processes. A limit of 221 GeV is set on the scale |F|1/2 in supersymmetric models. For 4, 6, and 8 extra dimensions, model-dependent limits on the fundamental mass scale MD of 0.55, 0.58, and …

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Mylanodon Rosei, A New Metacheiromyid (Mammalia: Palaeanodonta) From The Late Tiffanian (Late Paleocene) Of Northwestern Wyoming, Ross Secord, Philip D. Gingerich, Jonathan I. Bloch Dec 2002

Mylanodon Rosei, A New Metacheiromyid (Mammalia: Palaeanodonta) From The Late Tiffanian (Late Paleocene) Of Northwestern Wyoming, Ross Secord, Philip D. Gingerich, Jonathan I. Bloch

Department of Earth and Atmospheric Sciences: Faculty Publications

Mylanodon rosei is a new genus and species of late Paleocene metacheiromyid palaeanodont from a new late Tiffanian locality, Y2K Quarry, in the Clarks Fork Basin, Wyoming. The type is an adult dentary with P4 and a molariform double-rooted M1. This provides the first evidence that molariform teeth were retained in early Metacheiromyidae. A second specimen is a juvenile dentary with a partial P3 and an unerupted P4. This is the first juvenile dentition known for a Paleocene metacheiromyid. The new specimens enable determination of dental homologies. Reduction of teeth in early metacheiromyids took …

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Perturbative Calculation Of The Triply Differential Cross Section For Photo-Double-Ionization Of He, Andrei Y. Istomin, N. L. Manakov, Anthony F. Starace Dec 2002

Perturbative Calculation Of The Triply Differential Cross Section For Photo-Double-Ionization Of He, Andrei Y. Istomin, N. L. Manakov, Anthony F. Starace

Anthony F. Starace Publications

Single-photon, two-electron ionization of He is analyzed, taking into account electron correlation using lowest-order perturbation theory and including all individual electron angular momenta in the final two-electron continuum. Perturbative account of electron correlation in the final state, which describes the so-called TS-1 mechanism of double photoionization, combined with a variational account of electron screening, is found to provide results for the triply differential cross section at an excess energy of 20 eV that are in excellent agreement with both absolute experimental data and results of non-perturbative calculations, for all kinematics of the process in which the TS-1 mechanism is expected …

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Market Reports Dec 2002

Market Reports

Cornhusker Economics

No abstract provided.

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Differential Lipid Biosynthesis Underlies A Tradeoff Between Reproduction And Fl Ight Capability In A Wing-Polymorphic Cricket, Zhangwu Zhao, Anthony J. Zera Dec 2002

Differential Lipid Biosynthesis Underlies A Tradeoff Between Reproduction And Fl Ight Capability In A Wing-Polymorphic Cricket, Zhangwu Zhao, Anthony J. Zera

Anthony Zera Publications

The biochemical basis of life-history tradeoffs is a poorly studied aspect of life-history evolution. We used radiotracer and endocrine approaches to investigate the extent to which morphs of a wing-polymorphic insect differ in the biosynthesis of lipid classes important for dispersal capability vs. reproduction (ovarian growth). The flightcapable genotype of Gryllus firmus biosynthesized a greater amount of total lipid and triglyceride (main flight fuel), which was preferentially allocated to somatic tissue during early adulthood. By contrast, the fl ightless genotype biosynthesized a significantly greater amount of phospholipid (important in egg development), which was preferentially allocated to ovaries. Topical application of …

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Search For The Production Of Single Sleptons Through R-Parity Violation In Pp̅ Collisions At √S = 1.8 Tev, V. M. Abazov, Gregory R. Snow, D0 Collaboration Dec 2002

Search For The Production Of Single Sleptons Through R-Parity Violation In Pp̅ Collisions At √S = 1.8 Tev, V. M. Abazov, Gregory R. Snow, D0 Collaboration

Gregory Snow Publications

We report the first search for supersymmetric particles via s-channel production and decay of smuons or muon sneutrinos at hadronic colliders. The data for the two-muon and two-jets final states were collected by the D0 experiment and correspond to an integrated luminosity of 94 ± 5 pb-1. Assuming that R parity is violated via the single coupling λ′211, the number of candidate events is in agreement with expectation from the standard model. Exclusion contours are given in the (m0; m½) and (mΧ̅,mν planes for λ′ …

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A New Genus Of Flea Beetles From The Greater Antilles (Coleoptera: Chrysomelidae), Alexander S. Konstantinov Dec 2002

A New Genus Of Flea Beetles From The Greater Antilles (Coleoptera: Chrysomelidae), Alexander S. Konstantinov

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The new genus Normaltica and two new species (N. obrieni from Puerto Rico and N. iviei from the Dominican Republic) are described and illustrated. A key and illustrations of these taxa are pro- vided. Morphological consequences of wing reduction are discussed. Variability of N. obrieni is illustrated and discussed.

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Two-Photon Bremsstrahlung Processes In Atoms: Polarization Effects And Analytic Results For The Coulomb Potential, A. A. Krylovetskiĭ, N. L. Manakov, S. I. Marmo, Anthony F. Starace Dec 2002

Two-Photon Bremsstrahlung Processes In Atoms: Polarization Effects And Analytic Results For The Coulomb Potential, A. A. Krylovetskiĭ, N. L. Manakov, S. I. Marmo, Anthony F. Starace

Anthony F. Starace Publications

The partial wave analysis of two-photon free–free (bremsstrahlung) electron transition cross sections during scattering by a static potential U(r), as well as by an atom with a nonzero angular momentum, is carried out. The dipole interaction with radiation is taken into account in the second order of perturbation theory for the general case of elliptic polarization of photons. The polarization and angular dependences of the twophoton potential scattering amplitude is presented as a combination of the scalar product of electron momenta and photon polarization vectors and five atomic parameters containing Legendre polynomials of the scattering angle as …

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Almagest, Randall Snyder Dec 2002

Almagest, Randall Snyder

Randall Snyder Compositions

For Flute, Oboe, Bb Clarinet, Eb Alto Saxophone, Horn in F, Bassoon.

1975/2002

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Search For Minimal Supergravity In Single-Electron Events With Jets And Large Missing Transverse Energy In Pp̅ Collisions At √S =1.8 Tev, V. M. Abazov, Gregory R. Snow, D0 Collaboration Dec 2002

Search For Minimal Supergravity In Single-Electron Events With Jets And Large Missing Transverse Energy In Pp̅ Collisions At √S =1.8 Tev, V. M. Abazov, Gregory R. Snow, D0 Collaboration

Gregory Snow Publications

We describe a search for evidence of minimal supergravity (MSUGRA) in 92.7 pb-1 of data collected with the DØ detector at the Fermilab Tevatron pp̅ collider at√s =1.8 TeV. Events with a single electron, four or more jets, and large missing transverse energy were used in this search. The major backgrounds are from W +jets, misidentified multijet, tt̅, and WW production. We observe no excess above the expected number of background events in our data. A new limit in terms of MSUGRA model parameters is obtained.

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Livestock Checkoff Cases, J. David Aiken Dec 2002

Livestock Checkoff Cases, J. David Aiken

Cornhusker Economics

Over the past thirty-five years, Congress has authorized generic promotion programs, known as checkoff programs, for a variety of agricultural commodities. Within the past year, the beef checkoff has been invalidated, the pork checkoff has been invalidated, and the legality of the beef checkoff has been upheld. The two cases invalidating checkoff programs relied on U.S. Supreme Court rulings that checkoff programs violated the producers free speech rights. The one case ruling the beef checkoff constitutional characterized the checkoff advertising program as government speech, which is exempt from constitutional free speech protections. This article takes a look at legal issues …

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Dec 2002, Tractor Museum Dec 2002

Dec 2002, Tractor Museum

Lester F. Larsen Tractor Museum: Planning and Development Documents

Report from Director on Leave Development Projects Liason Report

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Lake-Catchment Interactions With Climate In The Low Arctic Of Southern West Greenland, N. John Anderson, Sherilyn C. Fritz, Christopher E. Gibson, Bent Hasholt, Melanie J. Leng Dec 2002

Lake-Catchment Interactions With Climate In The Low Arctic Of Southern West Greenland, N. John Anderson, Sherilyn C. Fritz, Christopher E. Gibson, Bent Hasholt, Melanie J. Leng

Department of Earth and Atmospheric Sciences: Faculty Publications

Arctic hydrology plays a central role in the earth’s heat balance and ocean circulation (Vörösmarty et al. 2001). Future changes associated with human influence on the climate system are also predicted to cause major changes in the energy and hydrologic mass balance of Arctic catchments. Climate change will likely affect permafrost and snowmelt, which dominate Arctic hydrology and control the chemistry of surface runoff (and hence streams and lakes) as water percolates through the active layer. However, the controls and dynamic impact of snowmelt are poorly understood, because this critical timeframe is often missed by sampling programs. In the Søndre …

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Milesian Decrees Of Isopoliteia And The Refoundation Of The City, Ca. 479 Bce, Vanessa B. Gorman Dec 2002

Milesian Decrees Of Isopoliteia And The Refoundation Of The City, Ca. 479 Bce, Vanessa B. Gorman

Department of History: Faculty Publications

In summary, the events described in Herodotus 6.19.2–22.1 are not only plausible, but entirely consistent with the physical and epigraphic evidence from Miletos. Persia sacked the city and killed or relocated the entire population. For the next fifteen years, while Persia still clung to Ionia, some settlers may have returned from among those who fled the sack or were absent abroad at the time. They settled cautiously on Kalabaktepe, awaiting events. When the Battle of Mykale and subsequent events pushed the Persian authority out of Ionia, the settlers looked to restore their once-famous city to something of its former size …

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Taxonomy And Distribution Of Butterflies (Papilionoidea) Of The Skardu Region, Pakistan, Muhammad Abbas, Muhammad Ather Rafi, Mian Inayatullah, Muhammad Rafique Khan, Harry Pavulaan Dec 2002

Taxonomy And Distribution Of Butterflies (Papilionoidea) Of The Skardu Region, Pakistan, Muhammad Abbas, Muhammad Ather Rafi, Mian Inayatullah, Muhammad Rafique Khan, Harry Pavulaan

The Taxonomic Report of the International Lepidoptera Survey

A study was conducted in six different localities around Skardu, Pakistan to document the butterfly fauna of that region. The study revealed that 16 species in 5 families and 14 genera occur in the area. The families include: Papilionidae (represented only by the genus Parnassius); Pieridae (genera Pieris, Pontia, and Colias are represented); Lycaenidae (genera Lycaena, Everes, Aricia, Plebejus, Zizeeria, and Zizina are represented); Nymphalidae (only two genera, Aglais and Cynthia are represented); and Satyridae (represented by the genera Pararge and Maniola).

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Breed Comparisons Of Angus, Charolais, Hereford, Jersey, Limousin, Simmental, And South Devon For Weight, Weight Adjusted For Body Condition Score, Height, And Body Condition Score Of Cows, Jesus Arango, L. V. Cundiff, L. Dale Van Vleck Dec 2002

Breed Comparisons Of Angus, Charolais, Hereford, Jersey, Limousin, Simmental, And South Devon For Weight, Weight Adjusted For Body Condition Score, Height, And Body Condition Score Of Cows, Jesus Arango, L. V. Cundiff, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Breed means and differences for weight (CW, n = 19,851), height (CH, n = 14,553), and condition scores (CS, n = 19,536) recorded in four seasons per year were evaluated for 881 cows ranging from 2 to 7 yr of age from Cycle I of the Germplasm Evaluation Program at the U.S. Meat Animal Research Center. Cows were straightbred Herefords and Angus and top-crosses from mating of Hereford, Angus, South Devon, Jersey, Simmental, Limousin, and Charolais sires to Hereford and Angus dams. The model included cow age, season of measurement, and their interactions, with year of birth, pregnancy-lactation (PL) code, …

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Comparisons Of Angus-, Braunvieh-, Chianina-, Hereford-, Gelbvieh-, Maine Anjou-, And Red Poll-Sired Cows For Weight, Weight Adjusted For Body Condition Score, Height, And Body Condition Score, Jesus Arango, L. V. Cundiff, L. Dale Van Vleck Dec 2002

Comparisons Of Angus-, Braunvieh-, Chianina-, Hereford-, Gelbvieh-, Maine Anjou-, And Red Poll-Sired Cows For Weight, Weight Adjusted For Body Condition Score, Height, And Body Condition Score, Jesus Arango, L. V. Cundiff, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Data from Angus, Hereford, and topcross cows (n = 641) from 2- to 8-yr-old daughters of seven breeds of sires included in Cycle II of the Germplasm Evaluation Program at the U.S. Meat Animal Research Center, comprising cow weight (CW, n = 15,698), height (CH, n = 15,676), and condition score (CS, n = 15,667), were used to estimate breed-group differences. Data were recorded in four seasons of each year (1975 to 1982). The mixed model included cow age, season of measurement, and their interactions, year of birth, pregnancy-lactation code (PL), and breed-group as fixed effects for CW and CS. …

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Estimates Of Genetic Parameters And Genetic Change For Reproduction, Weight, And Wool Characteristics Of Columbia Sheep, Kathryn J. Hanford, L. Dale Van Vleck, G. D. Snowder Dec 2002

Estimates Of Genetic Parameters And Genetic Change For Reproduction, Weight, And Wool Characteristics Of Columbia Sheep, Kathryn J. Hanford, L. Dale Van Vleck, G. D. Snowder

Department of Animal Science: Faculty Publications

Genetic parameters from both single-trait and bivariate analyses for prolificacy, weight and wool traits were estimated using REML with animal models for Columbia sheep from data collected from 1950 to 1998 at the U.S. Sheep Experiment Station (USSES), Dubois, ID. Breeding values from both single-trait and seven-trait analyses calculated using the parameters estimated from the single-trait and bivariate analyses were compared with respect to genetic trends. Number of observations were 31,401 for litter size at birth and litter size at weaning, 24,741 for birth weight, 23,903 for weaning weight, 29,572 for fleece weight and fleece grade, and 2,449 for staple …

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Genetic Parameters For Weight, Weight Adjusted For Body Condition Score, Height, And Body Condition Score In Beef Cows, Jesus Arango, Larry V. Cundiff, L. Dale Van Vleck Dec 2002

Genetic Parameters For Weight, Weight Adjusted For Body Condition Score, Height, And Body Condition Score In Beef Cows, Jesus Arango, Larry V. Cundiff, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Weight (CW, n = 61,798), weight adjusted for condition score (WA), hip height (CH, n = 56,494), and condition score (CS, n = 61,434) of cows (2 through 8 yr of age) produced by crosses of 22 sire breeds with Angus and Hereford dams in the first four cycles of the Germplasm Evaluation (GPE) Program at the U.S. Meat Animal Research Center were used to estimate genetic parameters with REML. The model included sire breed, dam breed, age in years, season of measurement (1 to 4) and their interactions, and year of birth and pregnancy-lactation code (PL) as fixed effects …

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Breed Comparisons Of Angus, Brahman, Hereford, Pinzgauer, Sahiwal, And Tarentaise For Weight, Weight Adjusted For Condition Score, Height, And Body Condition Score, Jesus Arango, L. V. Cundiff, L. Dale Van Vleck Dec 2002

Breed Comparisons Of Angus, Brahman, Hereford, Pinzgauer, Sahiwal, And Tarentaise For Weight, Weight Adjusted For Condition Score, Height, And Body Condition Score, Jesus Arango, L. V. Cundiff, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Data from topcross cows (n = 468) from six breeds of sire (Angus, Brahman, Hereford, Pinzgauer, Sahiwal, Tarentaise) and two breeds of dam (Angus and Hereford) of Cycle III of the Germplasm Evaluation (GPE) program at the U.S. Meat Animal Research Center (MARC) comprising cow weight (CW, n = 9,012), height (CH, n = 9,010), and condition score (CS, n = 8,991) recorded in four seasons per year from 2 to 6 yr of age were used to estimate breed-group differences. The mixed models included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and …

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Estimates Of Parameters Between Direct And Maternal Genetic Effects For Weaning Weight And Direct Genetic Effects For Carcass Traits In Crossbred Cattle, R. K. Splan, Larry V. Cundiff, M. E. Dikeman, L. Dale Van Vleck Dec 2002

Estimates Of Parameters Between Direct And Maternal Genetic Effects For Weaning Weight And Direct Genetic Effects For Carcass Traits In Crossbred Cattle, R. K. Splan, Larry V. Cundiff, M. E. Dikeman, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Estimates of heritabilities and genetic correlations were obtained for weaning weight records of 23,681 crossbred steers and heifers and carcass records from 4,094 crossbred steers using animal models. Carcass traits included hot carcass weight; retail product percentage; fat percentage; bone percentage; ribeye area; adjusted fat thickness; marbling score, Warner- Bratzler shear force and kidney, pelvic and heart fat percentage. Weaning weight was modeled with fixed effects of age of dam, sex, breed combination, and birth year, with calendar birth day as a covariate and random direct and maternal genetic and maternal permanent environmental effects. The models for carcass traits included …

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Blackbirds And Corn In Ohio, Richard A. Dolbeer Dec 2002

Blackbirds And Corn In Ohio, Richard A. Dolbeer

USDA Wildlife Services: Staff Publications

Damage to corn by blackbirds (Icteridae) has been an economic problem throughout historical times in North America. Ohio, with the highest nesting season population density of red-winged blackbirds (Agelaius phoeniceus) in North America and large acreages of corn, has been a key State in this conflict. Surveys of damage from 1968 to 1979 revealed that blackbirds annually destroyed less than 1% of the corn crops in Ohio, a 4- to 6-million dollar loss at 1979 prices. This total dollar loss is somewhat misleading because of the uneven distribution of damage among fields. Over 97% of the cornfields in Ohio receive …

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Evaluation Of Lasers To Disperse American Crows, Corvus Brachyrhynchos, From Urban Night Roosts, W.P. Gorenzel, B. F. Blackwell, G.D. Simmons, T.P. Salmon, R.A. Dolbeer Dec 2002

Evaluation Of Lasers To Disperse American Crows, Corvus Brachyrhynchos, From Urban Night Roosts, W.P. Gorenzel, B. F. Blackwell, G.D. Simmons, T.P. Salmon, R.A. Dolbeer

USDA Wildlife Services: Staff Publications

American crows (Corvus brachyrhynchos) have a long history of causing agricultural damage in North America. Shooting and bombing at crow night roosts have been employed to reduce such damage. Most roosts were located in rural locations, but in the latter half of the 1900s crows began to roost in urban locations. Urban crow roosts are presently a nationwide problem in the United States. Thousands of crows at a roost create problems for businesses and residents. Improved control techniques are needed. Lasers have been used in Europe to scare and disperse birds but the technique has only recently received formal testing. …

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A New Twist To Regional Economic Development, Cheryl A. Burkhart-Kriesel Dec 2002

A New Twist To Regional Economic Development, Cheryl A. Burkhart-Kriesel

Cornhusker Economics

Regional collaboration has always sounded like a good idea. The challenge was that it was hard to implement. From a business perspective, it was easier and more cost effective to make adjustments to your own business plan without asking for added input from other regional businesses, agencies and institutions. When times are good, you have the luxury of working independently. However, when you are faced with a general downturn in the economy and increased global competition, as many of the business sectors are today, you look hard for other ways of increasing efficiency. Some methods that could be explored may …

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The Insulation Of Genes From External Enhancers And Silencing Chromatin, Bonnie Burgess-Beusse, Catherine Farrell, Miklos Gaszner, Michael Litt, Vesco Mutskov, Felix Recillas - Targa, Melanie Simpson, Adam West, Gary Felsenfeld Dec 2002

The Insulation Of Genes From External Enhancers And Silencing Chromatin, Bonnie Burgess-Beusse, Catherine Farrell, Miklos Gaszner, Michael Litt, Vesco Mutskov, Felix Recillas - Targa, Melanie Simpson, Adam West, Gary Felsenfeld

Department of Biochemistry: Faculty Publications

Insulators are DNA sequence elements that can serve in some cases as barriers to protect a gene against the encroachment of adjacent inactive condensed chromatin. Some insulators also can act as blocking elements to protect against the activating influence of distal enhancers associated with other genes. Although most of the insulators identified so far derive from Drosophila, they also are found in vertebrates. An insulator at the 5′ end of the chicken β -globin locus marks a boundary between an open chromatin domain and a region of constitutively condensed chromatin. Detailed analysis of this element shows that it possesses …

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Initiative 300 And The Structure Of Nebraska’S Cattle Feeding Industry: Did The Law Make A Difference?, Azzeddine Azzam Dec 2002

Initiative 300 And The Structure Of Nebraska’S Cattle Feeding Industry: Did The Law Make A Difference?, Azzeddine Azzam

Cornhusker Economics

In a recently completed study on the effect of Initiative 300 on the structure of Nebraska's cattle feeding industry, agricultural economists, Azzeddine M. Azzam from the University of Nebraska-Lincoln and John R. Schroeter from Iowa State University, in cooperation with UNL Ag Law specialist, David Aiken, found no statistical difference between how the size of feedlots has evolved in Nebraska and how they've evolved in other major cattle feeding states (Colorado, Kansas and Texas) that have no restrictions on corporate investment in cattle feeding. All four states studied have seen a trend of larger feedlots supplanting small-scale operations. The study …

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Selection For Maternal Behavior In Mice—Direct And Correlated Responses, Chung-Feng Chiang, Rodger K. Johnson, Merlyn K. Nielsen Dec 2002

Selection For Maternal Behavior In Mice—Direct And Correlated Responses, Chung-Feng Chiang, Rodger K. Johnson, Merlyn K. Nielsen

Department of Animal Science: Faculty Publications

Three generations of divergent selection for a maternal care index (MCI) in mice were practiced in each of three replicates. MCI was recorded for 29–32 dams and litters in each replicate/line subclass. Pups of the top (or bottom) 6– 10 dams in each replicate, depending on number of females in the litters, were selected in high (H) and low (L) MCI lines. At birth, litters with more than 10 pups were standardized to 10 pups whereas litters with no more than 10 pups remained intact. On day 4, 8, 12, and 16 of lactation, time budgets during a 30-min session …

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Diagnostic Assays Based On Esterase-Mediated Resistance Mechanisms In Western Corn Rootworms (Coleoptera: Chrysomelidae), Xuguo Zhou, Michael E. Scharf, Srinivas Parimi, Lance J. Meinke, Robert J. Wright, Laurence D. Chandler, Blair D. Siegfried Dec 2002

Diagnostic Assays Based On Esterase-Mediated Resistance Mechanisms In Western Corn Rootworms (Coleoptera: Chrysomelidae), Xuguo Zhou, Michael E. Scharf, Srinivas Parimi, Lance J. Meinke, Robert J. Wright, Laurence D. Chandler, Blair D. Siegfried

Department of Entomology: Faculty Publications

Resistance to methyl-parathion among Nebraska western corn rootworm, Diabrotica virgifera virgifera LeConte, populations is associated with increased hydrolytic metabolism of an organophosphate insecticide substrate. An electrophoretic method to identify resistant individuals based on the staining intensity of esterase isozymes on nondenaturing polyacrylamide gels was developed. Three groups of esterases (I, II, and III) were visible on the gels, but only group II esterase isozymes were intensified in resistant populations. A total of 26 and 31 field populations of western corn rootworms from Nebraska (in 1998 and 1999, respectively) were assessed with nondenaturing polyacrylamide gel electrophoresis (PAGE) assays and diagnostic concentration …

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