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Northern Illinois University

Theses/Dissertations

1994

Cloned geminivirus DNA

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Restriction Mapping And Infectivity Of Cloned Geminivirus Dna, Jay C. Olaszek Jan 1994

Restriction Mapping And Infectivity Of Cloned Geminivirus Dna, Jay C. Olaszek

Honors Capstones

The bipartite ‘geminivirus -Squash Leaf Curl Virus (SLCV) has been the subject of several recent studies. Occuring in several strains, the strain known as SLCV-C is the object of this study. Since the virus is familiar to the discipline of plant virology but has not been largely considered in an experimental capacity when present in this isolate, this study endeavored to determine to which of the previously-studied strains SLCV-C appears most similar. Major aspects of the study were concerned with the determination of the physical nature of the strain, including the construction of a restriction endonuclease map and a test …