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Aspects Of Searching And Tunneling Behavior In Resource Utilization By The Formosan Subterranean Termite, Coptotermes Formosanus Shiraki., Justin Carl Hedlund
Aspects Of Searching And Tunneling Behavior In Resource Utilization By The Formosan Subterranean Termite, Coptotermes Formosanus Shiraki., Justin Carl Hedlund
LSU Historical Dissertations and Theses
Formosan subterranean termites, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), were acclimated with different masses of pine or no wood (control) and then allowed access to a thin sand filled tunneling chamber. As available pine increased, termite survival and food consumption increased but search tunnel volume decreased. Increased food size resulted in termites excavating fewer exploratory tunnels due to decreases in secondary and tertiary branch tunnels. An increase in food size also decreased the total length of a search tunnel network, due to decreases in primary and secondary branch order lengths. Search tunnel networks had proportionally more length devoted to primary tunnels …
Reproductive Isolation Between Two Species Of Topminnow, Fundulus Olivaceus And F. Euryzonus., Thomas Allen Blanchard
Reproductive Isolation Between Two Species Of Topminnow, Fundulus Olivaceus And F. Euryzonus., Thomas Allen Blanchard
LSU Historical Dissertations and Theses
Fundulus olivaceus and F. euryzonus are sister species that commonly occur syntopically in the Amite and Tangipahoa River drainages in Louisiana and Mississippi. Although the habitats of the two species are quite similar, and the timing of their reproductive activity overlaps extensively, no natural hybrids between them have been documented. I examined physiological, as well as behavioral, factors that could potentially serve as effective isolating mechanisms between them. The courtship and spawning behaviors of the two species were relatively generalized and showed a number of similarities. Under forced mating conditions in which females were allowed to associate with heterospecific males …
The Interactions Among The Subunits Of The Mcrbc Restriction System In E. Coli., Loganayaki Ganesh
The Interactions Among The Subunits Of The Mcrbc Restriction System In E. Coli., Loganayaki Ganesh
LSU Historical Dissertations and Theses
The McrBC system of Escherichia coli K12 restricts DNA containing modified cytosines within specific sites. The modified cytosines recognized may be 5-hydroxymethylcytosine, N$\sp4$-methylcytosine or 5-methylcytosine. In the mcrBC operon, the mcrB gene is expressed to produce the McrB$\sb{\rm L}$ protein (51 kDa) and the McrB$\sb{\rm S}$ protein (33kDa), which is translated from an inframe internal translation start site and lacks the N-terminal 161 amino acids of the long product. The McrC protein is a 39kDa protein and is required for the cleavage of most methylated substrates. Using a MalE-McrB$\sb{\rm L}$ fusion protein, the endonucleolytic McrB$\sb{\rm L}$ subunit was shown to …
Intraspecific Variation In Three Marsh Grasses In Response To Increased Flooding And Salinity., Jeannine Marie Lessmann
Intraspecific Variation In Three Marsh Grasses In Response To Increased Flooding And Salinity., Jeannine Marie Lessmann
LSU Historical Dissertations and Theses
Intraspecific variation in response to moderate levels of flooding and salinity stress was identified in Spartina patens, S. alterniflora, and Panicum hemitomon. This analysis enabled genotypes of each species to be selected for desired traits to enhance their function in restored systems, such as increased belowground biomass to stabilize soil substrates or increased aboveground biomass to enhance sedimentation. It was also determined that increased flood tolerance conferred a greater advantage for plant growth at moderate stress levels than did increased salt tolerance. However, this growth advantage was overridden by high salinity stress, and was greatly reduced by excessive flooding stress. …
Pest Status Of The Fall Armyworm, Spodoptera Frugiperda (J. E. Smith), On Conventional And Transgenic Cotton Expressing The Bacillus Thuringiensis Cryia (C) Delta-Endotoxin: Development, Survival, And Host-Strain Influence., John Joseph Adamczyk Jr
Pest Status Of The Fall Armyworm, Spodoptera Frugiperda (J. E. Smith), On Conventional And Transgenic Cotton Expressing The Bacillus Thuringiensis Cryia (C) Delta-Endotoxin: Development, Survival, And Host-Strain Influence., John Joseph Adamczyk Jr
LSU Historical Dissertations and Theses
The pest status of the fall armyworm, Spodoptera frugiperda (J. E. Smith), including development and survival on conventional and transgenic Bacillus thuringiensis (Bt) cotton and host-strain influences, was examined in laboratory and field studies. Neonates (1st instars) were fed leaves from conventional (cv. DP 5415 and cv. DP 5690) and transgenic Bt (cv. NuCOTN 33$\rm\sp{B}$ and cv. NuCOTN 35$\rm\sp{B})$ cotton. There were no significant differences $(P>0.05)$ between cultivars in larval mortality and survival to the pupal and adult stages. However, times to pupation and adult eclosion were significantly longer $(P<0.05)$ for larvae fed transgenic Bt cotton compared to conventional cotton. In addition, larvae weighed significantly less $(P<0.05)$ when fed transgenic Bt cotton compared to conventional cotton. A study was conducted to determine when cotton bolls become tolerant to fall armyworm injury. Fifth instars were caged individually on bolls of various ages from conventional (cv. DP 5415) and transgenic Bt (cv. NuCOTN 33$\rm\sp{B})$ cotton to define the period of boll susceptibility to larval injury. For DP 5415 bolls, there was no significant linear relationship $(P>0.05)$ describing larval mortality or successful boll penetration as …
Identification Of Antigens Of Edwardsiella Ictaluri, The Causative Agent Of Enteric Septicemia Of Catfish (Esc)., Michelle Marie Moore
Identification Of Antigens Of Edwardsiella Ictaluri, The Causative Agent Of Enteric Septicemia Of Catfish (Esc)., Michelle Marie Moore
LSU Historical Dissertations and Theses
Edwardsiella ictaluri, causative agent of enteric septicemia of catfish (ESC), is the primary bacterial pathogen of commercially produced channel catfish (Ictalurus punctatus). The purpose of this study was to make progress towards development of a successful ESC vaccine by generating and characterizing a pool of E. ictaluri antigens and determining if they were protective against ESC in catfish. Antigenic protein expression was evaluated in E. ictaluri cells under different conditions of growth using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and western blotting with pooled convalescent catfish serum (CCS). Results showed protein bands separated by one-dimensional PAGE may consist of one or …
Comparative Structure And Function Of Intrinsic Gill Muscles In Freshwater Bivalve Molluscs., Scott Medler
Comparative Structure And Function Of Intrinsic Gill Muscles In Freshwater Bivalve Molluscs., Scott Medler
LSU Historical Dissertations and Theses
The lamellibranch gill is used for various vital functions ranging from food capture to ion regulation. The foundation for many of these functions is the transport of water through the gill. This dissertation examines the organization of intrinsic gill muscles and the associated connective tissue in the freshwater bivalve Dreissena polymorpha with comparisons made to Corbicula fluminea and Toxolasma texasensis. Gill muscles can be divided into two groups: those associated with the connective tissue sheets that underlie external and internal gill epithelia; and those encased in connective tissue bands oriented perpendicular to the bases of gill filaments. The sets of …
Sample-Based Forest Landscape Diversity Indices., Bernard Ross Parresol
Sample-Based Forest Landscape Diversity Indices., Bernard Ross Parresol
LSU Historical Dissertations and Theses
Studies of spatial patterns of landscapes are useful to quantify human impact, predict wildlife effects, or describe various landscape features. A robust landscape index should quantify two distinct components of landscape diversity: composition and configuration. One category of landscape index is the contagion index. A generalized measure of contagion is defined as a function of concentration. From this definition two contagion indices, $\Gamma\sb1$ (a new index) and $\Gamma\sb2$ (an entropy formulation), are derived from expected values of geometric random variables. A widely used relative contagion index, $RC\sb2,$ is shown to be a scaled version of $\Gamma\sb2.$. Distributional properties of $\\Gamma\sb1,\ …
Behavior Of Red Snapper, Lutjanus Campechanus, In Relation To Trawl Modifications To Reduce Shrimp Trawler Bycatch., Donna Reeve Rogers
Behavior Of Red Snapper, Lutjanus Campechanus, In Relation To Trawl Modifications To Reduce Shrimp Trawler Bycatch., Donna Reeve Rogers
LSU Historical Dissertations and Theses
Behavioral responses of young-of-the-year red snapper, Lutjanus campechanus, to alterations in water flow were examined in the laboratory and the field in an effort to facilitate their release from shrimp trawls. Experiments with red snapper, collected by divers, were conducted in a racetrack flume. Four vertical and two horizontal mesh and solid panel types were examined. Four snapper (out of 96) escaped with vertical panels and thirteen fish (out of 48) exited with horizontal panels. Escapes occurred primarily with solid panels containing 0.95-cm holes drilled at 5-cm intervals. However, snapper that escaped with solid horizontal panels frequently went back into …
Materials Fluxes Across Different Scales Of Time And Space In The Rhone Delta, France., Philippe Francois Hensel
Materials Fluxes Across Different Scales Of Time And Space In The Rhone Delta, France., Philippe Francois Hensel
LSU Historical Dissertations and Theses
A study of materials fluxes across different scales of time and space was conducted in the Rhone Delta, France, to test the applicability of a conceptual model of deltaic ecosystem function in a scenario of accelerated sea-level rise to a mediterranean delta which has been heavily altered by human activities. Natural pulsing events such as tides, river floods and storms cause sediment surfaces to maintain elevation in the face of relative sea level rise through the process of soil formation. These pulses also enhance ecosystem function through habitat creation, food production, materials transformation, water quality and storm and flood protection. …
Cloning And Identification Of Chlamydomonas Reinhardtii Genes And Proteins Upregulated Under Low Carbon Dioxide Conditions., Aravind Somanchi
Cloning And Identification Of Chlamydomonas Reinhardtii Genes And Proteins Upregulated Under Low Carbon Dioxide Conditions., Aravind Somanchi
LSU Historical Dissertations and Theses
A mechanism that concentrates CO$\sb2$ in cells of Chlamydomonas reinhardtii, is induced when cells are grown at low CO$\sb2$ conditions. This mechanism increases the CO$\sb2$ concentration at the site of RuBisCO, and thereby improves the efficiency of C$\rm\sb{i}$ uptake and fixation. Cells adapting to low CO$\sb2$ show significant physiological changes. Few of the genes encoding proteins needed to produce these changes have been identified. A cDNA library has been constructed from C. reinhardtii to identify genes involved in these changes. The cloning and identification of several cDNAs that are upregulated under low CO$\sb2$ conditions are reported here. The possible roles …
The Role Of Hyperinsulinemia, The Sympathetic Nervous System And Nitric Oxide In Cardiovascular Function During The Development Of Obesity-Induced Hypertension., Agatha T. Borne
LSU Historical Dissertations and Theses
The interaction of hyperinsulinemia, the sympathetic nervous system (SNS) and nitric oxide (NO) was examined in lean and obese unilaterally lumbar sympathectomized dogs. Six lean and six obese dogs were studied while on a treadmill, during hyperinsulinemic euglycemic clamp (HIEC) procedures with and without concurrent nitric oxide synthase (NOS) inhibition with nitro-L-arginine (LNA). Changes in plasma insulin, femoral vascular resistance (FVR), heart rate (HR) and mean arterial pressure (MAP) were measured prior to and during a 7-week overfeeding period, and response to LNA administration was examined in the lean and obese state. Body weight increased from 19.9 $\pm$ 0.70 to …
Detection Of Mechanisms Of Profenofos Resistance In The Tobacco Budworm, Heliothis Virescens (F.)., John Ambrose Harold
Detection Of Mechanisms Of Profenofos Resistance In The Tobacco Budworm, Heliothis Virescens (F.)., John Ambrose Harold
LSU Historical Dissertations and Theses
Biochemical and physiological mechanisms of profenofos resistance in laboratory and field populations of the tobacco budworm, Heliothis virescens (F.) were studied. High frequencies of profenofos resistance were recorded in all field-collected strains and were strongly correlated with esterase (EST) activity and moderately correlated with glutathione S-transferase activity. Mean levels of acetylcholinesterase (AChE) sensitivity to inhibition by chlorpyrifos oxon were lower in some field strains than levels in an insecticide-susceptible strain; however, AChE sensitivity and resistance were poorly correlated in all field strains. There was no correlation between resistance and the activities of P450-monooxygenase or glutathione S-transferase measured. Studies with laboratory-selected …
The Role Of Alginate Lyase In Pseudomonas Syringae Pathovar Phaseolicola., Charlie Mark Ott
The Role Of Alginate Lyase In Pseudomonas Syringae Pathovar Phaseolicola., Charlie Mark Ott
LSU Historical Dissertations and Theses
The plant pathogen responsible for halo blight in legumes, Pseudomonas syringae pathovar phaseolicola, produces an alginate exopolysaccharide which prevents desiccation and enhances the "water soaking" effect associated with pathogenesis. These bacteria demonstrated elevated extracellular alginate lyase activity during log growth and stationary phases in batch cultures. An analysis of the substrate specificity indicated enzymatic activity on both acetylated and non-acetylated alginate. The release of the enzyme(s) in log growth phase and the subsequent decrease in production in stationary phase have not been previously reported. Extracellular activity during rapid cell growth was confirmed by continuous culture in both nutrient broth and …
Temporal Variation In Larval Fish Condition Indices., Michael John Burger
Temporal Variation In Larval Fish Condition Indices., Michael John Burger
LSU Historical Dissertations and Theses
A sensitive methodology for the determination of RNA and DNA concentrations was developed which allowed for the quantification of nanogram amounts of both DNA and RNA. The method uses a combination of thiazole orange, a non-specific nucleic acid stain, and the RNA degrading enzyme RNase. Thiazole orange had high fluorescence yield. The fluorescence was additive for combinations of RNA and DNA. The addition of proteinase-K increased the fluorescence of the tissue sample by 100%. Recovery of nucleic acid standards added to tissue extracts yielded $\sim$100% recovery. The method is compatible with assays for protein concentration and electron transport system (ETS) …
Development Of A Quantitative Polymerase Chain Reaction Procedure For Analysis Of Cytokine Responses To Strongylus Vulgaris In Ponies., Cyprianna Ellen Swiderski
Development Of A Quantitative Polymerase Chain Reaction Procedure For Analysis Of Cytokine Responses To Strongylus Vulgaris In Ponies., Cyprianna Ellen Swiderski
LSU Historical Dissertations and Theses
The effect of oral vaccination with radiation-attenuated Strongylus vulgaris larvae on the cytokine responses of ponies to oral challenge with S. vulgaris was examined. Message was quantified using RT-PCR by interpolation against a plasmid-derived standard curve. PCR product was detected using the Perkin-Elmer QPCR System 5000. Standard curves were analyzed with a two parameter exponential rise to maximum fit of the data. By altering the cycling parameters to maintain $\beta$-actin amplification within its exponential phase, $\beta$-actin was used as a normalization factor. The assay resolves 2 fold changes in message, has a linear dynamic range on the order of 3 …
Characterization Ofp53 Gene Sequence In Exons 5-8 Of The Western Mosquito Fish, Gambusia Affinis., Neeraja Kondapi Erraguntla
Characterization Ofp53 Gene Sequence In Exons 5-8 Of The Western Mosquito Fish, Gambusia Affinis., Neeraja Kondapi Erraguntla
LSU Historical Dissertations and Theses
Cancer is a multistage disease that involves both genetic and epigenetic factors. The number of physical and chemical agents with which human beings come in contact on a regular basis is increasing everyday. Somatic mutations can result due to such exposures and trigger uncontrolled cell division resulting in cancer. The p53 tumor suppressor gene has been identified in several vertebrate species ranging from man to fish. In addition, inactivation of p53 has been observed in a wide variety of tumors. Fish models are becoming increasingly popular for assessing environmental exposure. Low background incidence of mutations, relatively low cost tumor studies, …
Ecology And Loss Assessment Of Selected Homopteran Pests On Sugarcane: Interactions With The Fire Ant, Solenopsis Wagneri Santschi., Andrew Evans Woolwine
Ecology And Loss Assessment Of Selected Homopteran Pests On Sugarcane: Interactions With The Fire Ant, Solenopsis Wagneri Santschi., Andrew Evans Woolwine
LSU Historical Dissertations and Theses
Field studies in 1995, 1996, and 1997, using insecticides to alter population densities of yellow sugarcane aphid, Sipha flava (Forbes), West Indian canefly, Saccharosydne saccharivora (Westwood) and the pink sugarcane mealybug, Saccharicoccus sacchari (Cockerell), indicated that sugarcane infested by these insects did not suffer sugar quality loss (as measured by brix, polarity, sucrose, purity, and theoretical recoverable sugar (TRS)). There was no apparent indication that Homoptera stressed sugarcane (indicated by concentrations of free amino acids in extracted sugarcane juice). A very slight, but significant increase in the percent brix was observed in sugarcane juice extracted from stalks washed free of …
Purification Of Porphobilinogen Deaminase And The Sequencing And Expression Of Thehemc Gene Of Rhodobacter Capsulatus., Keith A. Canada
Purification Of Porphobilinogen Deaminase And The Sequencing And Expression Of Thehemc Gene Of Rhodobacter Capsulatus., Keith A. Canada
LSU Historical Dissertations and Theses
Rhodobacter capsulatus uses the common tetrapyrrole pathway to synthesize heme, bacteriochlorophyll, siroheme and vitamin B-12. As part of our studies on the regulation of this pathway, the product of the hemC gene, porphobilinogen deaminase was purified approximately 200-fold. The molecular weight, isoelectric point, pH optimum and Km of the enzyme were all similar to other porphobilinogen deaminases. The stoichiometry of the enzyme was found not to be the expected four porphobilinogens used per one uroporphyrin formed. Rather a 9:1 ratio was typically seen. Further study of this atypical ratio uncovered evidence of possible in vivo regulation of porphobilinogen deaminase dependant …
Moth Diversity In Louisiana Longleaf Pine Savannas And Mixed Mesophytic Hardwood Forests: Species Composition And Management Effects., Deborah Landau
Moth Diversity In Louisiana Longleaf Pine Savannas And Mixed Mesophytic Hardwood Forests: Species Composition And Management Effects., Deborah Landau
LSU Historical Dissertations and Theses
Two habitats, a mixed hardwood forest and a longleaf pine savanna, were selected for analysis of moth composition. Three studies assessed differences between moth diversity, efficiency of sampling intensities, and effects of management practices in these habitats. The first study compared diversity and community composition between the two habitats based on 4,337 moths representing 394 species. The mixed hardwood habitat contained 314 species and the longleaf pine 208. Species overlap between two longleaf pine sites was as low as overlap between longleaf pine and mixed hardwood habitats (38%). Differences in moth richness between habitats may be a reflection of differences …
Airbirds: Adaptative Strategies To The Aerial Lifestyle From A Life History Perspective., Manuel Marin-Aspillaga
Airbirds: Adaptative Strategies To The Aerial Lifestyle From A Life History Perspective., Manuel Marin-Aspillaga
LSU Historical Dissertations and Theses
"Airbirds" are defined as those birds that are adapted to almost constant life on the wing and do not perch, except for breeding and roosting. They use the air as the only medium in which to find and gather food. One taxon meets that definition: the swifts (Apodidae). Foraging zones of airbirds can be segregated in terms of clutch size, commuting foraging distance, nestling period, and nestling feeding rate. This study looked at foraging behavior and nestling diet, incubation patterns and mode of development in cypseloidine swifts, that as a group has extreme life history patterns. In a temperate one-egg …
Biology And Ecology Of The Anopheles Quadrimaculatus Sibling Species Complex In Louisiana., Cynthia Roxanne Rutledge
Biology And Ecology Of The Anopheles Quadrimaculatus Sibling Species Complex In Louisiana., Cynthia Roxanne Rutledge
LSU Historical Dissertations and Theses
A polymerase chain reaction method for identifying individuals in the Anopheles quadrimaculatus Say sibling species complex was validated for wild mosquitoes from Louisiana and Mississippi. This method distinguished An. quadrimaculatus species A, B, C, and D by detecting species-specific differences in the 2nd internal transcribed spacer of ribosomal DNA and was 100% specific and 95% sensitive. A polymerase chain reaction assay that detects differences in the second internal transcribed spacer of ribosomal DNA was tested for its usefulness in identifying all immature stages of two representatives in the An. quadrimaculatus sibling species complex. The technique was successful in amplifying DNA …
The Effects Of Pah-Contaminated Sediments On Benthic-Feeding Fishes And Their Associated Prey., Candace Gay Hinkle-Conn
The Effects Of Pah-Contaminated Sediments On Benthic-Feeding Fishes And Their Associated Prey., Candace Gay Hinkle-Conn
LSU Historical Dissertations and Theses
Subtidal sediments near Port Fourchon, Louisiana, USA, have been contaminated with produced water, as indicated by the levels of polycyclic aromatic hydrocarbons (PAH) for over four decades. Studies were undertaken to examine the effect of produced water/PAH on the potential for trophic transfer (from meiofauna and small macrofauna to benthic-feeding fishes) of sediment-bound contaminants. A survey of meiofaunal communities along a putative pollution gradient from the produced water outfall to a distance of 1 km found that meiofauna densities were significantly higher at the most contaminated sites ($\sim$10,000 ng PAH g$\sp{-1}$ dry sediment). The community at the most contaminated sites, …
Serological And Molecular Biological Studies Of Marine Predator-Prey Systems., Marilyn J. Hoyt
Serological And Molecular Biological Studies Of Marine Predator-Prey Systems., Marilyn J. Hoyt
LSU Historical Dissertations and Theses
Two aspects of species identification were addressed; a molecular approach to identify anchovy larval and serological methods for prey detection in predator gut washes. Analysis of the pattern of restriction fragments of mitochondrial DNA (mtDNA) from adult anchovies from the Gulf of Mexico was employed to speciate four anchovy species. MtDNA isolated from white muscle of adult Anchoa mitchilli, A. hepsetus, A. nasuta and Engraulis eurystole produced an amplified 2.4 Kb DNA product when primers for NADH dehydrogenase 5 and 6 genes were used in polymerase chain reactions (PCR). Digestion of the amplified sequence with Dpn II produced a unique …
Characterization Of Seven Transmembrane Domain Receptor Gene Expression In Olfactory Receptor Neurons., Kathryn Medler
Characterization Of Seven Transmembrane Domain Receptor Gene Expression In Olfactory Receptor Neurons., Kathryn Medler
LSU Historical Dissertations and Theses
The expression of 7 transmembrane domain receptors in olfactory receptor neurons of the channel catfish, Ictalurus punctatus was characterized. Due to disagreements in the literature as to how many odorant receptor genes are expressed in olfactory neurons, this study directly measured the number of odorant receptor gene transcripts expressed in single olfactory neurons. Individual olfactory receptor neurons can express more than one receptor, with some neurons expressing at least 3 to 4 receptors. These findings correlate with electrophysiological evidence but disagree with conclusions based on in situ hybridization. Receptors similar to odorant receptors are also expressed in taste buds, indicating …
Early Fertilization Events In The Freshwater Zebra Mussel, Dreissena Polymorpha., Michael James Misamore
Early Fertilization Events In The Freshwater Zebra Mussel, Dreissena Polymorpha., Michael James Misamore
LSU Historical Dissertations and Theses
The morphological and functional mechanisms involved in the early fertilization events in the freshwater zebra mussel Dreissena polymorpha (Bivalvia:Veneroida:Dreissenidae) are presented. Mature adults release gametes in response to external applications of 5-hydroxytryptamine. Sperm retain a primitive morphology consisting of a head, midpiece and tail The head is approximately 1.6 $\mu$m in diameter and 4 $\mu$m in length. An acrosomal vesicle is highly compartmentalized with a central axial core containing actin. The midpiece is composed of 4 mitochondria and a pair of centrioles. A single flagellum is elaborated from the distal centriole. The eggs of D. polymorpha are 50-60 $\mu$m in …
Cerebral Hemoglobin Oxygen Saturation In Patients With Restless Legs Syndrome And Periodic Limb Movements In Sleep., Mark Jude Hurry
Cerebral Hemoglobin Oxygen Saturation In Patients With Restless Legs Syndrome And Periodic Limb Movements In Sleep., Mark Jude Hurry
LSU Historical Dissertations and Theses
Pathogenic theories of Restless Legs Syndrome (RLS) and Periodic Limb Movements in Sleep (PLMS) were reviewed and critiqued, and a new theory for the pathogenesis of RLS and PLMS was presented. It was proposed that both disorders are caused by sleep-related hypoperfusion in the area of the cerebral cortex that mediates sensation and movement in the legs. The hypoperfusion was proposed to be caused by high amplitude oscillations in total cerebral blood flow known as B-waves and by flow-reducing turbulence at several of the bifurcations in the anterior cerebral artery. This theory was tested by using near infrared spectroscopy (NIRS) …
The Role Of Curved Dna In Bacillus Subtilis Rna Polymerase-Promoter Interactions., Murad Mohammed Odeh
The Role Of Curved Dna In Bacillus Subtilis Rna Polymerase-Promoter Interactions., Murad Mohammed Odeh
LSU Historical Dissertations and Theses
Intrinsically curved DNA upstream of promoters was shown to affect the Bacillus subtilis RNA polymerase-promoter interactions leading to transcription initiation. The Alu156, a B. subtilis bacteriophage dependent on the curved DNA located immediately upstream of the $-$35 region, and modified forms of Alu156 were used. Promoters were examined using gel retardation and DNase I footprinting assays. DNase I footprinting analysis of the Alu156 promoter demonstrated a unique pattern of DNase I hypersensitive and protected sites characteristic of DNA wrapped around the protein. This wrapping was observed upstream of the curved DNA. To examine the nature of curved DNA, the native …
Dynamics And Management Of Insecticide Resistance In The Horn Fly, Haematobia Irritans Irritans (L.) (Diptera: Muscidae)., Antonio Thadeu Medeiros De Barros
Dynamics And Management Of Insecticide Resistance In The Horn Fly, Haematobia Irritans Irritans (L.) (Diptera: Muscidae)., Antonio Thadeu Medeiros De Barros
LSU Historical Dissertations and Theses
Studies on resistance of the horn fly, Haematobia irritans irritans (L.), to insecticides were conducted in Louisiana from 1989 to 1998. These studies included monitoring resistance and resistance dynamics under different strategies of insecticide use, monitoring the efficacy of insecticide products, establishing a relationship between bioassay findings and product efficacy, and evaluation of a new insecticide class. In four years (1989--1992), efficacy of 20% diazinon-impregnated car tags used yearly was reduced from greater than twenty to just one week of control, and tag failure was observed with resistance ratios (RR) from 1.8 to 5.7. Diazinon resistance developed more slowly and …