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A Nuclear Juvenile Hormone-Binding Protein From Larvae Of Manduca Sexta: A Putative Receptor For The Metamorphic Action Of Juvenile Hormone, Subba R. Palli, Kazushige Touhara, Jean-Philippe Charles, Bryony C. Bonning, Jeffrey K. Atkinson, Stephen C. Trowell, Kiyoshi Hiruma, Walter G. Goodman, Themis Kyriakides, Glenn D. Prestwich, Bruce D. Hammock, Lynn M. Ridiford
A Nuclear Juvenile Hormone-Binding Protein From Larvae Of Manduca Sexta: A Putative Receptor For The Metamorphic Action Of Juvenile Hormone, Subba R. Palli, Kazushige Touhara, Jean-Philippe Charles, Bryony C. Bonning, Jeffrey K. Atkinson, Stephen C. Trowell, Kiyoshi Hiruma, Walter G. Goodman, Themis Kyriakides, Glenn D. Prestwich, Bruce D. Hammock, Lynn M. Ridiford
Bryony C. Bonning
A 29-kDa nuclear juvenile hormone (JH)-binding protein from the epidermis of Manduca sexta larvae was purified by using the photoaffinity analog for JH II ([3H]epoxyhomofarnesyldiazoacetate) and partially sequenced. A 1.1-kb cDNA was isolated by using degenerate oligonucleotide primers for PCR based on these sequences. The cDNA encoded a 262-amino acid protein that showed no similarity with other known proteins, except for short stretches of the interphotoreceptor retinoid-binding protein, rhodopsin, and human nuclear protein p68. Recombinant baculovirus containing this cDNA made a 29-kDa protein that was covalently modified by [3H]epoxyhomofarnesyldiazoacetate and specifically bound the natural enantiomer of JH I (Kd = …
Insect Control By Use Of Recombinant Baculoviruses Expressing Juvenile Hormone Esterase, Bryony C. Bonning, Bruce D. Hammock
Insect Control By Use Of Recombinant Baculoviruses Expressing Juvenile Hormone Esterase, Bryony C. Bonning, Bruce D. Hammock
Bryony C. Bonning
Anti-juvenile hormone activity, and the resulting cessation of feeding, has long been considered by the agricultural chemical industry as a means for insect control. Juvenile hormone esterase (JHE) contributes to the decline in JH titer at certain times during larval development. This chapter reviews the efforts made to develop a fast acting recombinant baculovirus insecticide by creating a virus which will express artificially high levels of JHE in the insect at inappropriate times. The findings are discussed in the light of our current understanding of the regulation of larval development and the role of JHE. Prospects for the newly developed, …
Ligand Binding By A Recombinant Insect Juvenile Hormone Binding Protein, Kazushige Touhara, Keith A. Lerro, Bryony C. Bonning, Bruce D. Hammock, Glenn D. Prestwich
Ligand Binding By A Recombinant Insect Juvenile Hormone Binding Protein, Kazushige Touhara, Keith A. Lerro, Bryony C. Bonning, Bruce D. Hammock, Glenn D. Prestwich
Bryony C. Bonning
A cDNA for the hemolymph juvenile hormone binding protein (JHBP) of larval Manduca sexta has been isolated, sequenced, and expressed in an insect cell line. A recombinant baculovirus, containing the JHBP cDNA fused to the p10 promoter of Autographa californica nuclear polyhedrosis virus, was constructed. Insect cells (Sf9) infected with this virus secreted recombinant JHBP (rJHBP) into the medium (>50 pg/mL), and cotranslational removal of an 18 amino acid leader sequence was observed. rJHBP was cross-reactive with an antiserum prepared to the hemolymph JHBP and was specifically labeled by [3H]EHDA, a photoaffinity analog of JH 11, demonstrating that rJHBP …
Interaction Of Insecticide Resistance Genes In Field Populations Of Culex Pipiens (Diptera: Culicidae) From Italy In Response To Changing Insecticide Selection Pressure, Bryony C. Bonning, J. Hemingway, R. Romi, G. Majori
Interaction Of Insecticide Resistance Genes In Field Populations Of Culex Pipiens (Diptera: Culicidae) From Italy In Response To Changing Insecticide Selection Pressure, Bryony C. Bonning, J. Hemingway, R. Romi, G. Majori
Bryony C. Bonning
Culex pipiens Linnaeus larvae were collected from various locations in Italy and colonized as separate strains. These were analysed for elevated nonspecific esterase activity and frequency of altered acetylcholinesterase (AChE) mechanisms of insecticide resistance, and bioassayed, to define the cross-resistance spectra conferred by these to organophosphorus and carbamate insecticides. These mechanisms were further characterized by polyacrylamide gel electrophoresis. Elevated esterase A1 (formerly known as Est 3A) which predominated in C. pipiens from Italy in 1985 had been replaced by two esterases, A2 and B2. Altered acetylcholinesterase was still present at high frequencies. Altered and normal acetylcholinesterase were distinguished by differential …
Possible Selective Advantage Of Anopheles Spp. (Diptera: Culicidae) With The Oxidase- And Acetylcholinesterase-Based Insecticide Resistance Genes After Exposure To Organophosphates Or An Insect Growth Regulator In Sri Lankan Rice Fields, J. Hemingway, Bryony C. Bonning, K. G. I. Jayawardena, I. S. Weerasinghe, P. R. J. Herath, H. Oouchi
Possible Selective Advantage Of Anopheles Spp. (Diptera: Culicidae) With The Oxidase- And Acetylcholinesterase-Based Insecticide Resistance Genes After Exposure To Organophosphates Or An Insect Growth Regulator In Sri Lankan Rice Fields, J. Hemingway, Bryony C. Bonning, K. G. I. Jayawardena, I. S. Weerasinghe, P. R. J. Herath, H. Oouchi
Bryony C. Bonning
Treatment of rice fields in Sri Lanka with monocrotophos at 10 mg/litre or pirimiphos-methyl at 0·1 mg/litre gave a selective advantage to larvae of Anopheles subpictus Grassi and A. nigerrimus Giles carrying the oxidase- and acetylcholinesterase-based resistance genes, respectively. However, this selective advantage was apparent for less than ten days after spraying. There was no mortality of any larval instar with monocrotophos 12 days after spraying, and no fourth-instar larval mortality with pirimiphos-methyl 17 days after spraying. The chemical degredation curves for these compounds indicated that this short duration of efficacy was due to the instability of the compounds in …