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The Application Of Weak-Anion Exchange Chromatography For The Analysis Of Organic Zwitterions Using Lc/Ms/Ms, Michael Jason Bishop
The Application Of Weak-Anion Exchange Chromatography For The Analysis Of Organic Zwitterions Using Lc/Ms/Ms, Michael Jason Bishop
Chemistry Theses
A rapid and accurate quantitative method was developed and validated for the analysis of four urinary organic acids with nitrogen containing functional groups, formiminoglutamic acid (FIGLU), pyroglutamic acid (PYRGLU), 5-hydroxyindoleacetic acid (5-HIAA), and 2-methylhippuric acid (2-METHIP) by liquid chromatography tandem mass spectrometry (LC/MS/MS). The chromatography was developed using a weak anion-exchange amino column that provided mixed-mode retention of the analytes. The elution gradient relied on changes in mobile phase pH over a concave gradient, without the use of counter-ions or concentrated salt buffers. A simple sample preparation was used, only requiring the dilution of urine prior to instrumental analysis. The …