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Evaluation Of Chitosan And Collagen As Scaffolding For A Tissue Engineered Aortic Heart Valve, Steven Christopher Waller
Evaluation Of Chitosan And Collagen As Scaffolding For A Tissue Engineered Aortic Heart Valve, Steven Christopher Waller
Theses and Dissertations
Children born with congenital heart valve defects require open-heart surgery to implant an artificial replacement valve. These valves are unable to grow with the developing child and need replacing every 5 years. Tissue engineered heart valves, capable of growing with the patient, would alleviate the need for repeat surgery. I hypothesize chitosan and collagen possess advantageous qualities as scaffolding for a tissue engineered heart valve. This study evaluated chitosan and collagen hydrogels as potential scaffold materials. Chitosan scaffolds had suitable pore size/distribution and scaffold strength; however, they were unable to sustain cell attachment or viability. Collagen gels were assessed for …
Preparation And Characterization Of Particles From Chitosan With Different Molecular Weights And Their Trimethyl Chitosan Derivatives For Nasal Immunization, W Boonyo, H E. Junginger, N Waranuch, A Polnok, T Pitaksuteepong
Preparation And Characterization Of Particles From Chitosan With Different Molecular Weights And Their Trimethyl Chitosan Derivatives For Nasal Immunization, W Boonyo, H E. Junginger, N Waranuch, A Polnok, T Pitaksuteepong
Journal of Metals, Materials and Minerals
According to previous studies, molecular weights (MW) of chitosan presented an influence on the level of immune response to model antigen when administering with chitosan through nasal route. Moreover, trimethyl chitosan (TMC) with a degree of quaternization (DQ) of 40%, chitosan derivatives, was also shown to be the most potent nasal OVA delivery platforms compared to TMC with DQ of 20% and 60% in mice model. The aims of this study were to prepare particles from chitosan with difference MW and their TMC derivatives (DQ = 40%) and to characterize size, zeta potential, loading efficiency and release profile in order …
Preparation Of N-Acetyl-D-Glucosamine Using Enzyme From Aspergillus Sp., P Setthakaset, R Pichyangkura, A Ajavakom, M Sukwattanasinitt
Preparation Of N-Acetyl-D-Glucosamine Using Enzyme From Aspergillus Sp., P Setthakaset, R Pichyangkura, A Ajavakom, M Sukwattanasinitt
Journal of Metals, Materials and Minerals
N-acetyl-D-glucosamine (GlcNAc) is one of the basic constituents of mammal cartilage, which is known to help repair deteriorating cartilage and relieve pain and inflammation in osteoarthritis patients. The preparation of GlcNAc from squid pen β-chitin by using Aspergillus sp. chitinase in enzymatic hydrolysis is studied. The cultivation of the fungus in colloidal chitin minimum media at pH 3.5 and 40°C for 5 days gives the highest chitinolytic activity of 3.1 U/mL. The crude enzyme obtained from the the cultivation is used in the hydrolysis of milled squid pen β-chitin (50μm) at pH 4 (acetic acid 2M) and 45°C using 22 …
Surface Modification Of Bricks By Chitosan Coatings, S Nilpairach, S T. Dubas
Surface Modification Of Bricks By Chitosan Coatings, S Nilpairach, S T. Dubas
Journal of Metals, Materials and Minerals
Bricks were successfully modified by application of a chitosan coating in order to provide their surface with a water repellant character. The chitosan coating was prepared by depositing solutions of variable chitosan concentration (1% or 2.5% w/w) onto the brick surface and let to dry under ambient condition. In order to improve the water repellant property, the films were cross-linked with a 1% solution of sodium citrate, sodium sulfate or sodium tri - polyphosphate. The effect of the chitosan concentration on the morphology of the coating at the brick surface was observed by scanning electron microscope (SEM). It was found …
Improved L-929 Cell Growth From Self Assembled Pdadmac/Gelatin Thin Films, P Kittitheeranun, P Potiyaraj, T Bunaprasert, N Sanchavanakit, S T. Dubas
Improved L-929 Cell Growth From Self Assembled Pdadmac/Gelatin Thin Films, P Kittitheeranun, P Potiyaraj, T Bunaprasert, N Sanchavanakit, S T. Dubas
Journal of Metals, Materials and Minerals
This article demonstrates the advantages of using PDADMAC/gelatin over chitosan/gelatin multilayer thin films to improve L-929 mouse fibroblast growth. Although chitosan-based materials are often preferred in cell culture, we put in evidence the problematic low stability and decomposition of chitosan based film under physiological pH. Two kinds of coating were assembled using either chitosan or Poly(diallyldimethylammonium chloride) (PDADMAC) as polycationic polyelectrolyte and gelatin as the anionic counterpart. The non-toxic nature of each coating was confirmed by MTT assay for cell viability and cell proliferation. Using optical microscopy, it was observed that the L-929 fibroblast spread preferentially on the PDADMAC/gelatin surface …
Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck
Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck
Publications
Turmeric (Curcuma longa L.) plants that were grown in vitro for 17 or 22 weeks as a fed-batch culture in 2.5 L vessels yielded 39 to 43 g and 62 to 70 g of fresh rhizomes per vessel, respectively (95 % confidence interval). The MS liquid medium was maintained at 6 % sucrose through media addition twice during the experiment. Various methods were employed in attempts to increase secondary metabolism. Antioxidant and total phenolics assays were employed to characterize phytochemical activity. A first experiment exposed four clones to phenylalanine and/or methyl jasmonate (MeJa) from week 12 to 17 in …
Process For Formation Of Cationic Poly (Lactic-Co-Glycolic Acid) Nanoparticles Using Static Mixers, Yamuna Reddy Charabudla
Process For Formation Of Cationic Poly (Lactic-Co-Glycolic Acid) Nanoparticles Using Static Mixers, Yamuna Reddy Charabudla
University of Kentucky Master's Theses
Nanoparticles have received special attention over past few years as potential drug carriers for proteins/peptides and genes. Biodegradable polymeric poly (lactic-co-glycolic acid) (PLGA) nanoparticles are being employed as non-viral gene delivery systems for DNA. This work demonstrates a scalable technology for synthesis of nanoparticles capable of gene delivery. Cationic PLGA nanoparticles are produced by emulsiondiffusion- evaporation technique employing polyvinyl alcohol (PVA) as stabilizer and chitosan chloride for surface modification. A sonicator is used for the emulsion step and a static mixer is used for dilution in the diffusion step of the synthesis. A static mixer is considered ideal for the …
Durable And Reusable Antimicrobial Textiles, Leila Elizabeth Bonin
Durable And Reusable Antimicrobial Textiles, Leila Elizabeth Bonin
LSU Master's Theses
Antimicrobial textiles are a large research focus in the textile industry. There is an apparent need for creating reusable and durable antimicrobial textiles. Most of the textiles with antimicrobial properties effective against Staphylococcus aureus are disposable. To address the issue, two types of biopolymer solutions were created in a USDA lab. Both solutions displayed antimicrobial properties. A medium weight, plain weave cotton sheeting was used as the test fabric. Samples of the sheeting were cut, treated, and tested to determine their efficacy as antimicrobial textiles. The tests performed included tensile deformation, bending, shearing, compression, surface friction, surface roughness, and treatment …
The Synthesis Of Water Soluble N-Acyl Chitosan Derivatives For Characterization As Antibacterial Agents, Lakia Monique Champagne
The Synthesis Of Water Soluble N-Acyl Chitosan Derivatives For Characterization As Antibacterial Agents, Lakia Monique Champagne
LSU Doctoral Dissertations
Currently, natural polysaccharides are being utilized increasingly in the markets because they exhibit biodegradability, biocompatibility, versatility, and are found abundant in nature. The diversity of natural polysaccharides provides the chemist with a broad spectrum of raw materials that can be used in many biological applications. Chitosan is a natural polysaccharide that possesses excellent biological properties. It has been recognized for its antibacterial activity, in that it is destructive towards the bacterium Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The antibacterial property of chitosan can be enhanced by changing the hydrophobic/hydrophilic nature of the polysaccharide backbone. This research entails …