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Articles 1 - 21 of 21
Full-Text Articles in Entire DC Network
Biochemical Investigations Of Macular Degeneration: The Significance Of Protein Oxidation Including Novel Methods For Its Study, Sarah Warburton
Biochemical Investigations Of Macular Degeneration: The Significance Of Protein Oxidation Including Novel Methods For Its Study, Sarah Warburton
Theses and Dissertations
The retinal pigment epithelium (RPE) is a monolayer of cells located directly behind the photoreceptor cells in the retina. These cells are involved in a variety of functions that support the visual process in the eye, namely 1) they form a blood-retina barrier which separates the neural retina from the choroid's blood supply, 2) the apical processes of RPE cells diurnally phagocytose the outer segments of photoreceptor cells, and 3) they participate in the renewal of the photopigment 11-cis retinal. Age-related macular degneration (AMD) is the leading cause of blindness in people over the age of 50 years in North …
Fabrication Of Polymeric Microfluidic Devices For Protein Analysis, Jikun Liu
Fabrication Of Polymeric Microfluidic Devices For Protein Analysis, Jikun Liu
Theses and Dissertations
2-Bromoisobutyryl bromide was immobilized on poly(methyl methacrylate) (PMMA) substrates activated using an oxygen plasma. Atom-transfer radical polymerization was then performed to graft poly(ethylene glycol) (PEG) on the PMMA surface. PMMA micro capillary electrophoresis (µCE) devices made with the covalently modified surfaces exhibited substantially reduced electroosmotic flow and nonspecific adsorption of proteins. Both column efficiency and migration time reproducibility were one order of magnitude better with derivatized PMMA µCE devices compared to untreated versions. Fast, reproducible, and efficient separations of proteins and peptides were demonstrated using the PEG-grafted PMMA µCE chips. All analyses were completed in less than 60 seconds, and …
Electrically Charged Sol-Gel Coatings For On-Line Preconcentration And Analysis Of Zwitterionic Biomolecules By Capillary Electrophoresis, Wen Li
USF Tampa Graduate Theses and Dissertations
Novel on-line methods are presented for the extraction, preconcentration and analysis of zwitterionic biomolecules using sol-gel-coated columns coupled to a conventional UV/visible detector. The presented approaches do not require any additional modification of the commercially available standard CE instrument. Extraction, stacking, and focusing techniques were used in the preconcentration procedures. The positively charged sol-gel coatings were created using N-octadecyldimethyl[3-(trimethoxysilyl) proply]ammonium chloride (C18-TMS) in the coating sol solutions. Due to the presence of a positively charged quaternary ammonium moiety in C18-TMS, the resulting sol-gel coating carried a positive charge. The negatively charged sol-gel coatings were due to the presence of sulfonate …
Knowledge Based Structure Modeling Of The Third Hypervariable Region Of Antibodies, Kevin Galens
Knowledge Based Structure Modeling Of The Third Hypervariable Region Of Antibodies, Kevin Galens
Theses
Protein structure prediction has gained increased attention over the past decades in a wide range of biological disciplines. Creating an accurate visual model of a protein can aid in protein engineering; which has implications in the creation of therapeutic molecules as is the case with antibodies. The third complementarity determining region of the heavy chain of antibodies (CDR-H3) is known to show a large degree of variation in sequence and in length, and therefore has provided difficulties for structure prediction. By separating the CDR-H3 into two logical sections, the apex and base, and using a homology modeling techniques for each …
Thermodynamics Of Buried Water In Protein Cavities And Revised Algorithms For Introducing Polarizability To Molecular Dynamics Simulations, Lisa Renee Olano
Thermodynamics Of Buried Water In Protein Cavities And Revised Algorithms For Introducing Polarizability To Molecular Dynamics Simulations, Lisa Renee Olano
University of New Orleans Theses and Dissertations
Free energy calculations for the transfer of a water molecule from the pure liquid to an interior cavity site in a protein are presented. Three different protein cavities, in bovine pancreatic trypsin inhibitor (BPTI), the I106A mutant of lysozyme, and in the I76A mutant of barnase, represent very different environments for the water molecule, one which is polar, forming four water-protein hydrogen bonds, and two which are more hydrophobic, only forming one or two water-protein hydrogen bonds. The calculations give very different free energies for the different cavities, with only the polar BPTI cavity predicted to be hydrated. The corresponding …
In Vitro Expression And Purification Of Class I Mhc Molecules, Loi Cheng
In Vitro Expression And Purification Of Class I Mhc Molecules, Loi Cheng
Honors Scholar Theses
The major histocompatibility complex (MHC) is a gene family responsible for many critical functions of the immune system in most vertebrates. The MHC consists of three classes differentiated by their structure and function, and MHC class I encodes antigen binding proteins as well as chaperone and accessory proteins such as tapasin. The purpose of this project is to reconstitute several human MHC class I molecules in their peptide-filled and peptide-deficient forms, and to purify these proteins for biochemical study. The expressed proteins include wild type and mutant variants of the fusion protein human leukocyte antigen HLA-B*0801-fos, and human beta-2-microglobulin (β2m). …
Membrane-Based Protein Preconcentration Microfluidic Devices, Yi Li
Membrane-Based Protein Preconcentration Microfluidic Devices, Yi Li
Theses and Dissertations
Interest in microchip capillary electrophoresis (CE) is growing due to the rapid analysis times provided and small sample input requirements. However, higher-concentration samples are typically needed because of the small (~pL) detection volumes in these devices. I have made membrane-based protein preconcentration systems in capillary and microchip designs to increase the detectability of low-concentration biological samples. A photopolymerized ion-permeable membrane interfaced with a microchannel in poly(methyl methacrylate) (PMMA) formed the preconcentrator. When a voltage was applied between the sample reservoir and the ionically conductive membrane in a capillary-based system, R-phycoerythrin was concentrated more than 1,000 fold, as determined by laser-induced …
Factors Affecting The Site Of Investment, And The Reliance On Savings For Arctic Breeders: The Capital–Income Dichotomy Revisited, Marcel Klaassen, Kenneth F. Abraham, Robert L. Jefferies, Mark Vrtiska
Factors Affecting The Site Of Investment, And The Reliance On Savings For Arctic Breeders: The Capital–Income Dichotomy Revisited, Marcel Klaassen, Kenneth F. Abraham, Robert L. Jefferies, Mark Vrtiska
Nebraska Game and Parks Commission: Staff Research Publications
The extent to which migratory birds that breed in the Arctic and winter in southern biomes rely on residual body stores for reproduction is unresolved. The short arctic summer and the limited availability of food early in the season constrain the time available for successful reproduction. Birds that are able to bring sufficient endogenous reserves to the breeding ground to meet, at least partially, the demands of egg-laying can initiate clutch production soon after arrival, thereby shortening the length of the breeding season and improving the chances of reproductive success. The amount of reserves available will be influenced by body …
Analytical Potential Of Polymerized Liposomes Bound To Lanthanide Ions For Qualitative And Quantitative Analysis Of Proteins, Marina Santos
Analytical Potential Of Polymerized Liposomes Bound To Lanthanide Ions For Qualitative And Quantitative Analysis Of Proteins, Marina Santos
Electronic Theses and Dissertations
One of the intriguing features of biological systems is the prevalence of highly selective and often very strong interactions among different cellular components. Such interactions play a variety of organizational, mechanical, and physiological roles at the cellular and organism levels. Antigen-antibody complexes are representative examples of highly selective and potent interactions involving proteins. The marked specificity of protein-antibody complexes have led to a wide range of applications in cellular and molecular biology related research. They have become an integral research tool in the present genomic and proteomic era. Unfortunately, the production of selective tools based on antigen-antibody interactions requires cumbersome …
Transposon Disruption Of The Complex I Nadh Oxidoreductase Gene (Snod) In Staphylococcus Aureus Is Associated With Reduced Susceptibility To The Microbicidal Activity Of Thrombin-Induced Platelet Microbicidal Protein 1, Arnold S. Bayer, Peter Mcnamara, Michael R. Yeaman, Natalie Lucindo, Tiffanny Jones, Ambrose L. Cheung
Transposon Disruption Of The Complex I Nadh Oxidoreductase Gene (Snod) In Staphylococcus Aureus Is Associated With Reduced Susceptibility To The Microbicidal Activity Of Thrombin-Induced Platelet Microbicidal Protein 1, Arnold S. Bayer, Peter Mcnamara, Michael R. Yeaman, Natalie Lucindo, Tiffanny Jones, Ambrose L. Cheung
Dartmouth Scholarship
The cationic molecule thrombin-induced platelet microbicidal protein 1 (tPMP-1) exerts potent activity against Staphylococcus aureus. We previously reported that a Tn551 S. aureus transposon mutant, ISP479R, and two bacteriophage back-transductants, TxA and TxB, exhibit reduced in vitro susceptibility to tPMP-1 (tPMP-1(r)) compared to the parental strain, ISP479C (V. Dhawan, M. R. Yeaman, A. L. Cheung, E. Kim, P. M. Sullam, and A. S. Bayer, Infect. Immun. 65:3293-3299, 1997). In the current study, the genetic basis for tPMP-1(r) in these mutants was identified. GenBank homology searches using sequence corresponding to chromosomal DNA flanking Tn551 mutant strains showed that the fourth gene …
Targeting C-Reactive Protein For The Treatment Of Cardiovascular Disease, Mark B. Pepys, Gideon M. Hirschfield, Glenys A. Tennent, J Ruth Gallimore, Melvyn C. Kahan, Vittorio Bellotti, Philip N. Hawkins, Rebecca M. Myers, Martin D. Smith, Alessandra Polara, Alexander J. A Cobb, Steven V. Ley, J. Andrew Aquilina, Carol V. Robinson, Isam Sharif, Gillian A. Gray, Caroline A. Sabin, Michelle C. Jenvey, Simon E. Kolstoe, Darren Thompson, Stephen P. Wood
Targeting C-Reactive Protein For The Treatment Of Cardiovascular Disease, Mark B. Pepys, Gideon M. Hirschfield, Glenys A. Tennent, J Ruth Gallimore, Melvyn C. Kahan, Vittorio Bellotti, Philip N. Hawkins, Rebecca M. Myers, Martin D. Smith, Alessandra Polara, Alexander J. A Cobb, Steven V. Ley, J. Andrew Aquilina, Carol V. Robinson, Isam Sharif, Gillian A. Gray, Caroline A. Sabin, Michelle C. Jenvey, Simon E. Kolstoe, Darren Thompson, Stephen P. Wood
Faculty of Science - Papers (Archive)
Complement-mediated inflammation exacerbates the tissue injury of ischaemic necrosis in heart attacks and strokes, the most common causes of death in developed countries. Large infarct size increases immediate morbidity and mortality and, in survivors of the acute event, larger non-functional scars adversely affect long-term prognosis. There is thus an important unmet medical need for new cardioprotective and neuroprotective treatments. We have previously shown that human C-reactive protein (CRP), the classical acute-phase protein that binds to ligands exposed in damaged tissue and then activates complement1, increases myocardial and cerebral infarct size in rats subjected to coronary or cerebral artery ligation, respectively2,3. …
Identification Of Novel Allosteric Regulators Of Human Erythrocyte Pyruvate Kinase, Shilpa S. Kharalkar
Identification Of Novel Allosteric Regulators Of Human Erythrocyte Pyruvate Kinase, Shilpa S. Kharalkar
Theses and Dissertations
Erythrocyte pyruvate kinase (R-PK) is a key glycolytic enzyme catalyzing the transphosphorylation of phosphoenolpyruvate (PEP) and ADP to pyruvate and ATP respectively3,4. The substrate PEP and product pyruvate of this reaction are involved in a number of energetic and biosynthetic pathways; hence a tight regulation of R-PK activity is crucial not only for glycolysis, but also for the entire cellular metabolism. Deficiency of R-PK is one of the most common enzymatic defects of RBC, and may be caused by mutations of the PK-LR (pyruvate kinase liver red blood cell) gene31, 32. Clinically, R-PK deficiency manifests itself as a chronic life-long …
Importance Of Soy Protein And Isoflavone Intake For Protection Against Heart Disease, A Thorp, J Buckley, A Coates, Trevor A Mori, Jo Hodgson, Jackie Mansour, Peter Howe, Barbara Meyer
Importance Of Soy Protein And Isoflavone Intake For Protection Against Heart Disease, A Thorp, J Buckley, A Coates, Trevor A Mori, Jo Hodgson, Jackie Mansour, Peter Howe, Barbara Meyer
Faculty of Health and Behavioural Sciences - Papers (Archive)
Abstract presented at The 2006 Annual Scientific Meeting of the Nutrition Society of Australia, 29 November - 2 December, Sydney, Australia
Effects Of Corn Processing Method And Protein Concentration In Finishing Diets Containing Wet Corn Gluten Feed On Cattle Performance, C. N. Macken, G. E. Erickson, T. J. Klopfenstein, R. A. Stock
Effects Of Corn Processing Method And Protein Concentration In Finishing Diets Containing Wet Corn Gluten Feed On Cattle Performance, C. N. Macken, G. E. Erickson, T. J. Klopfenstein, R. A. Stock
Department of Animal Science: Faculty Publications
Three hundred twenty crossbred steer calves (308 kg) were used to determine the effects of corn processing and addition of urea on performance with diets containing wet corn gluten feed (WCGF). The treatment design was a 5 × 2 factorial with factors of corn processing (dryrolled, DRC; fine-ground, FGC; rolled high-moisture, RHMC; ground high-moisture, GHMC; or steam-flaked corn, SFC) and CP concentration (14 or 15%) with 4 pens per treatment and 8 steers per pen. The final diet contained 60% corn, 25% WCGF, 10% corn silage, and 5% supplement (DM basis). No significant protein × grain processing interactions occurred for …
Small Molecules Binding To Serpins, Junaid Afridi
Small Molecules Binding To Serpins, Junaid Afridi
Theses and Dissertations
Serpins are a unique breed of proteins due to their enzymatic mechanism. Two systems were closely monitored during fluorescent binding studies, the ACT-CHY along with the AT:TRY interaction. Four different conformational variants of each system were studied including the native, cleaved, latent and complex forms. Three different fluorescent dyes were used to identify the conformations including ANS, TNS, and bis-ANS. SI studies and protease assays utilizing both Suc-AAPF-pNA and L-BAPNA were instrumental in determining conformations along with gel electrophoresis studies. The hydrophobic dyes bound to the different serpins with varying KD and ΔFmax due to structural variations among the conformers …
Roles Of Intra-Fruit Oxygen And Carbon Dioxide In Controlling Pepper (Capsicum Annuum L.) Seed Development And Storage Reserve Deposition, J. Blasiak, Anxiu Kuang, Chakavak S. Farghangi, M. E. Musgrave
Roles Of Intra-Fruit Oxygen And Carbon Dioxide In Controlling Pepper (Capsicum Annuum L.) Seed Development And Storage Reserve Deposition, J. Blasiak, Anxiu Kuang, Chakavak S. Farghangi, M. E. Musgrave
Biology Faculty Publications and Presentations
Seeds developing within a locular space inside hollow fruit experience chronic exposure to a unique gaseous environment. Using two pepper cultivars, `Triton' (sweet) and `PI 140367' (hot), we investigated how the development of seeds is affected by the gases surrounding them. The atmospheric composition of the seed environment was characterized during development by analysis of samples withdrawn from the fruit locule with a gas-tight syringe. As seed weight plateaued during development, the seed environment reached its lowest O2 concentration (19%) and highest CO2 concentration (3%). We experimentally manipulated the seed environment by passing different humidified gas mixtures through the fruit …
Characterization Of The Tcof1 Gene Using A Neuroblastoma Cell Line And A Mouse Model, Lin Li
Characterization Of The Tcof1 Gene Using A Neuroblastoma Cell Line And A Mouse Model, Lin Li
Theses and Dissertations
Treacher Collins syndrome (TCS) is an autosomal dominant craniofacial development disorder and is caused by mutations in the TCOF1 gene. The TCOFl protein treacle is a nucleolar protein and may function in ribosome biogenesis.Previously, we identified downstream candidate genes using microarray analysis after manipulating Tcofl levels in a murine neuroblastoma (NB) cell line. The list of genes includes cell cycle genes as well as the transcription factors Cnbp and Tbx2, which are known to affect the cell cycle through the c-myc and p19-Mdm2-p53-p21 pathways respectively. To further characterize the cellular effects of Tcofl, stably transfected NB cell lines with overexpression …
Dual Regulation Of Telomerase Activity By Hsf1 And Its Role In Prostate Cancer Progression, Keith Douglas Ostergaard Jensen
Dual Regulation Of Telomerase Activity By Hsf1 And Its Role In Prostate Cancer Progression, Keith Douglas Ostergaard Jensen
Theses and Dissertations
It has been shown that the key components of the hsp90 chaperone complex, including hsp90, p23, hsp70, hsp40, and HOP (p60), associate with telomerase; however, their specific roles in telomerase function and tumor progression have not yet been defined. HSF1, the primary mammalian heat shock protein transcription factor, may affect telomerase activity and transformation by regulating the expression of several hsp90 chaperone complex proteins in response to stress as well as regulating the transcription of hTERT, the protein subunit of telomerase.In our in vitro model of prostate cancer progression, as cells progress from immortal but non-tumorigenic (P69) to tumorigenic (M2182) …
Biochemical Responses Of Dimorphic Seeds Of Arthrocnemum Indicum Willd. During Germination, Inhibition, And Alleviation Under Saline And Non-Saline Conditions, Zamin Shaheed Siddiqui
Biochemical Responses Of Dimorphic Seeds Of Arthrocnemum Indicum Willd. During Germination, Inhibition, And Alleviation Under Saline And Non-Saline Conditions, Zamin Shaheed Siddiqui
Turkish Journal of Biology
Dimorphic seeds of Arthrocnemum indicum (a halophytic shrub) exhibit diverse germination responses following salinity treatment and subsequent exposure to growth regulators by adjusting their physiological and biochemical processes. The present study examined the effects of 24-h and 72-h NaCl treatment on germination, germination velocity, and the associated biochemical changes in protein and RNA contents of 2 seed morphs, and compared these effects after transfer into distilled water or 10 mM thiourea solution. Treatment for 24 and 72 h in NaCl solution induced a significant reduction in the rate of germination and final percent germination of both seed morphs. No germination …
Molecular Viewer Using Spiegel, Pavani Baddepudi
Molecular Viewer Using Spiegel, Pavani Baddepudi
Theses
Study of nucleic acids and proteins has evoked great interest for its role in research involving areas such as human genome and drug design. Understanding the core structure and behavior of these protein molecules requires reading and analyzing large amounts of data about atoms derived from a plethora of experiments. In recent years, computer graphics has gained popularity for its ability to translate these large datasets into three dimensional structures providing easy visualization. This project has implemented one such 3-D molecular viewer to display different models of molecules and study their properties. The project was developed in Java3D using the …
Simulating Protein Evolution Via Thermodynamic Models, Yanlong Xu
Simulating Protein Evolution Via Thermodynamic Models, Yanlong Xu
LSU Doctoral Dissertations
Natural proteins are results of evolution and they need to maintain certain thermodynamic stabilities in order to carry out their biological functions. By simulating protein evolution based on thermodynamic rules, we could reconstruct the evolution trajectory and analyze the evolutionary dynamics of a protein population, and further understand the protein sequence-structure-function relationship. In this study, we have used both a simplified lattice model and a high-resolution atomic model to simulate protein evolution processes. With the lattice model, we have investigated general theoretical questions about how protein structural designability would affect protein evolution, particularly how it would affect protein recombination and …