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Genetically Modified Mosquitoes: A New Strategy To Control Mosquito Borne Diseases, Padet Siriyasatien, Usavadee Thavara

The Thai Journal of Veterinary Medicine

Mosquito borne diseases remain a major human and animal health concern in many areas of the world. Chemical control strategy faces a number of problems including the development of insecticide resistance in mosquitoes, the impact of insecticides on human and animal health and the environment and the constant inability to control mosquitoes effectively. Advances in the knowledge about mosquito-pathogen relationships and the molecular biology of mosquitoes make it now possible to produce mosquito strains that are unable to transmit various parasites or viruses. Strategies of using the genetically modified mosquitoes to control mosquito borne diseases are discussed here.

Bovine Immune Response To Shiga-Toxigenic Escherichia Coli O157:H7., Mark A Hoffman, Christian Menge, Thomas A Casey, William Laegreid, Brad T Bosworth, Evelyn A Dean-Nystrom

Manuscripts, Articles, Book Chapters and Other Papers

Although cattle develop humoral immune responses to Shiga-toxigenic (Stx+) Escherichia coli O157:H7, infections often result in long-term shedding of these human pathogenic bacteria. The objective of this study was to compare humoral and cellular immune responses to Stx+ and Stx- E. coli O157:H7. Three groups of calves were inoculated intrarumenally, twice in a 3-week interval, with different strains of E. coli: a Stx2-producing E. coli O157:H7 strain (Stx2+ O157), a Shiga toxin-negative E. coli O157:H7 strain (Stx- O157), or a nonpathogenic E. coli strain (control). Fecal shedding of Stx2+ O157 was significantly higher than that of Stx- O157 or the …

Transfusion-Associated Transmission Of West Nile Virus, United States 2003 Through 2005, Susan P. Montgomery, Jennifer A. Brown, Matthew Kuehnert, Theresa L. Smith, Nicholas Crall, Robert S. Lanciotti, Alexandre Macedo De Oliveira, Thomas Boo, Anthony A. Marfin, 2003 West Nile Virus Transfusion-Associated Transmission Investigation Team

Other Publications in Zoonotics and Wildlife Disease

BACKGROUND: National blood donation screening for West Nile virus (WNV) started in June 2003, after the documentation of WNV transfusion-associated transmission (TAT) in 2002.

STUDY DESIGN AND METHODS: Blood donations were screened with investigational nucleic acid amplification assays in minipool formats. Blood collection agencies (BCAs) reported screening results to state and local public health authorities. Donor test results and demographic information were forwarded to CDC via ArboNET, the national electronic arbovirus surveillance system. State health departments and BCAs also reported suspect WNV TATs to CDC, which investigated these reports to confirm WNV infection in blood transfusion recipients in the absence …

Culex Tritaeniorhynchus Is Unlikely To Be A Vector For The Porcine Reproductive And Respiratory Syndrome Virus (Prrsv), Kidsadagon Pringproa, Sudchit Chungpivat, Rapee Panyathong, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

At least, 4 mosquito species, Culex tritaeniorhynchus, Culex gelidus, Anopheles vagus and Mansonia uniformis were identified on a pig farm in Nakorn Pathom, Thailand between March 2004 to February 2005. C. tritaeniorhynchus was found to be the predominant species (88.387.63%). To determine whether C. tritaeniorhynchus could serve as a mechanical vector for PRRSV transmission, 2 experiments were conducted on: the duration of PRRSV within the mosquitoes and PRRSV transmission from the PRRSV-infected pigs to naive pigs by the infected mosquitoes. PRRSV nucleic acid could be detected in the whole pooled mosquito samples for up to 48 hours post feeding in …

Thoracoscopic-Guided Lung Biopsy In Dogs, Churee Pramatwinai, Atichat Brahmasa, Suwicha Chuthatep, Pasakorn Birksawan, Kiatpicet Komin

The Thai Journal of Veterinary Medicine

The study developed a thoracoscopic-guided lung biopsy technique that was able to be performed on the right or left lung lobes of dogs. Ten experimental dogs weighing 6-20 kilograms were anesthetized, ventilated and positioned in a lateral recumbency. Either the left or right hemithorax was approached. Two instrumental ports were utilized, the first port for a telescope was located at the fifth or sixth intercostal space at a half way distance between the vertebral column and sternum. The second port for the grasping forceps, 12-mm in diameter, was located at either the fourth or sixth or seventh intercostal space at …

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.

Rapid Isolation Of Dna From The Mucus Of Asian Arowana (Scleropages Formosus, Osteoglossidae), Nantarika Chansue

The Thai Journal of Veterinary Medicine

Owing to arowana being listed in CITES, DNA diagnostics should not rely on conventional methods of obtaining tissue samples. A non-invasive method of obtaining material containing DNA has been developed. Mucus samples for DNA isolation can be obtained by cotton swabbing the arowana body surface with subsequent purification of DNA fragments by using phenol/chloroform. DNA sample quality is assessed by UV absorbancy at wave lengths of 260 nm and 280 nm, as well as by calculating the absorbancy ratio involving 260 nm/280 nm results in order to determine DNA sample quality and quantity. Electrophoresis is employed to assess DNA quantity …

Morphological Changes In The Oviduct Of Culling Replacement Gilts, Paisan Tienthai, Kriengyot Sajjarengpong, Padet Talummuk

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the morphological changes of the oviduct from 30 culling replacement gilts and 6 control sows. Blood samples and female genital organs were taken immediately after slaughter. Post-mortem examinations were made on the genital organs and oviductal samples, i.e. utero-tubal junction (UTJ), isthmus and ampulla, were collected and fixed for histological analysis. Observations indicated that the reasons for culling the gilts were 'no estrus' (68%) and 'repeat breeding' (32%). The greatest pathological changes (67%) were found in repeat breeding gilts, whereas 52% of no estrous gilts were at prepubertal phase. The female hormonal …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Anusak Kijtawornrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Editor’S Note, Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

No abstract provided.

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1-Animal Health Surveillance Demands Continue to Grow Diagnostic News [Page] 1- Combined North American/European-like / LV “Multi-PRRSV Real-Time PCR Assay [Page] 2- Antemortem Diagnostics for Bovine Respiratory Disease: Obtaining Bronchoalveolar Lavage Samples [Page] 2- Holiday Hours Extension News [Page] 3- Tularemia Cases Diagnosed at SDSU ADRDL: September 2005 – October 2006 [Page] 4- Process Verified Programs (PVP’s) Available to SD Calf Producers [Page] 5- Pieces and Parts [Page] 8- Calendar of Events

Anti-Capsular Antibodies Activate Killing Of Escherichia Coli O8:K87 By The Alternate Complement Pathway In Porcine Serum, N. M. Clark, E. M. Berberov, M. Wang, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Enterotoxigenic Escherichia coli (ETEC) strains that produce K88 (F4) ⁺ fimbria are important causes of diarrhea and post-diarrheal septicemia in swine. ETEC O8:K87, a serotype represented by a number of these strains, is typically serum resistant. Strain-specific antibodies are known to activate alternative C pathway-mediated killing of other serum-resistant E. coli [Hill, A.W., Shears, A.L., Hibbitt, K.G., 1978. The requirement of specific antibody for the killing of E. coli by the alternate complement pathway in bovine serum. Immunology 34, 131–136], but their antigenic targets have not been determined. We tested the hypothesis that anti-K87 antibodies activate alternative pathway-mediated killing of …

Development Of A Gis-Based, Real-Time Internet Mapping Tool For Rabies Surveillance, Jesse D. Blanton, Arie Manangan, Jamie Manangan, Cathleen A. Hanlon, Dennis Slate, Charles E. Rupprecht

Other Publications in Zoonotics and Wildlife Disease

Background: Oral rabies vaccination programs have been implemented to control the spread of wildlife rabies in the United States. However, current surveillance systems are inadequate for the efficient management and evaluation of these large scale vaccine baiting programs. With this in mind, a GIS-based rabies surveillance database and Internet mapping application was created. This surveillance system, RabID, provides a new resource for the rapid mapping and dissemination of data on animal rabies cases in relation to unaffected, enzootic, and baited areas where current interventions are underway.

Results: RabID is a centralized database for diagnostic and demographic information collected by local, …

Sub-Typing Of Prrsv Isolates By Means Of Measurement Of Cross-Neutralization Reactions, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

The degree of similitude or closeness between two different isolates or strains of PRRSV is very important for deciding which strains should be used for immunization, by either vaccinating with commercial vaccines or attempting to stabilize a herd with planned wt PRRSV infection. As previously known for certain important viral diseases, such as in Foot and Mouth Disease, the reciprocal (cross-) neutralization titers between two strains may be of utmost importance to establish the degree of similarity or difference between these. There is currently a void of methods that would allow distinguishing or grouping strains of PRRSV in a manner …

Identificación Parasitaria De Monos Tití Cabeciblanco Saguinus Oedipus Del Proyecto Tití, Santa Catalina, Bolívar, Diana Marcela Barrera Pinillos

Medicina Veterinaria

Los parásitos de los Saguinus oedipus como los de los primates del nuevo mundo en general, han sido estudiados en primates en cautiverio, pero en vida silvestre son muy poco conocidos, particularmente los que son endémicos de primates en peligro; se presentan algunas limitaciones a la hora de tomar y preservar las muestras las cuales finalmente definen la calidad y confiabilidad de los resultados. Entre estas encontramos, los pequeños volúmenes de las deposiciones en muchas especies, la dificultad de colección dentro del bosque debido a los hábitos arbóreos de los micos, las variaciones de humedad y temperatura en el trópico, …

A Missense Mutation In Pmel17 Is Associated With The Silver Coat Color In The Horse, Emma Brunberg, Leif Andersson, Gus Cothran, Kaj Sandberg, Sofia Mikko, Gabriella Lindgren

Veterinary Science Faculty Publications

BACKGROUND: The Silver coat color, also called Silver dapple, in the horse is characterized by dilution of the black pigment in the hair. This phenotype shows an autosomal dominant inheritance. The effect of the mutation is most visible in the long hairs of the mane and tail, which are diluted to a mixture of white and gray hairs. Herein we describe the identification of the responsible gene and a missense mutation associated with the Silver phenotype.

RESULTS: Segregation data on the Silver locus (Z) were obtained within one half-sib family that consisted of a heterozygous Silver colored stallion with 34 …

Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff

Biological Sciences Faculty Publications

Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …

Preventing Back Pain In Horses, Jenifer Nadeau

Extension Articles

Back pain is a source of poor performance and many different types of horses suffer from back problems. Early recognition of back pain is important, since damage may be cumulative. This information is provided to help horse owners and riders prevent, recognize, and pursue treatment for back pain in their horses.

Comparative Analysis Of Vertebrate Eif2ak2 (Pkr) Genes And Assignment Of The Equine Gene To Eca15q24-Q25 And The Bovine Gene To Bta11q12-Q15, Andrey A. Perelygin, Teri L. Lear, Andrey A. Zharkikh, Margo A Brinton

Veterinary Science Faculty Publications

The structures of the canine, rabbit, bovine and equine EIF2AK2 genes were determined. Each of these genes has a 5' non-coding exon as well as 15 coding exons. All of the canine, bovine and equine EIF2AK2 introns have consensus donor and acceptor splice sites. In the equine EIF2AK2 gene, a unique single nucleotide polymorphism that encoded a Tyr329Cys substitution was detected. Regulatory elements predicted in the promoter region were conserved in ungulates, primates, rodents, Afrotheria (elephant) and Insectifora (shrew). Western clawed frog and fugu EIF2AK2 gene sequences were detected in the USCS Genome Browser and compared to those of other …

An ∼140-Kb Deletion Associated With Feline Spinal Muscular Atrophy Implies An Essential Lix1 Function For Motor Neuron Survival, John C. Fyfe, Marilyn Menotti-Raymond, Victor A. David, Lars Brichta, Alejandro A. Schaffer, R. Agarwala, William J. Murphy, William J. Wedemeyer, Brittany L. Gregory, Bethany G. Buzzell, Meghan C. Drummond, Brunhilde Wirth, Stephen J. O'Brien

Biology Faculty Articles

The leading genetic cause of infant mortality is spinal muscular atrophy (SMA), a clinically and genetically heterogeneous group of disorders. Previously we described a domestic cat model of autosomal recessive, juvenile-onset SMA similar to human SMA type III. Here we report results of a whole-genome scan for linkage in the feline SMA pedigree using recently developed species-specific and comparative mapping resources. We identified a novel SMA gene candidate, LIX1, in an ~140-kb deletion on feline chromosome A1q in a region of conserved synteny to human chromosome 5q15. Though LIX1 function is unknown, the predicted secondary structure is compatible with …

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.

The Study Of The Radiographic Positionings Of The Canine Elbow For Identifying The Medial Coronoid Process, Suwicha Chuthatep, Kongkiat Srisuwatanasagul, Chalika Wangdee, Kumpanart Sunthornwipart, Surassanan Angkana

The Thai Journal of Veterinary Medicine

Twenty cadaver canine forelimbs, 9 right and 11 left, were used to evaluate the most effective radiographic position that can enhance the cranial aspect of the medial coronoid process (MCP) of the ulna. A lead pellet (1 mm) was buried to represent the area representing the cartilegenous fragment in the canine patient in the fragmented medial coronoid process (FMCP). The distance between the pellet and the radial surface and the superimposition of the humeral condyle silhouette on the pellet were used as parameters for analysis. The results showed that the disto45 medialproximolateral oblique (Di45M-PrLO) position with a 40 flexed elbow …

Surveillance Of Classical Swine Fever Virus Antibody Titers In Sows And Their Piglets Under Different Vaccination Programs, Achariya Sailasuta, Rachod Tantilertcharoen, Pornchalit Ausawacheep, Sanipa Suradhat, Praveena Kitikoon, Supot Wattanapunsak, Sawang Kesdangsakonwut, Sumittra Wattanodorn, Supasawat Buranawej, Sudarat Damrongwatanapokin, Dumneon Chaturawitwong

The Thai Journal of Veterinary Medicine

Classical swine fever virus (CSFV), serum neutralizing antibody titers of 854 sows were determined using a neutralizing peroxidase linked assay (NPLA). Blood samples were collected one-week post-partum from 1st- 6th parity sows, from 30 swine fever-free farms. The farms were divided according to their vaccination programs, 10 farms for each program. Program A sows had been vaccinated 3 weeks pre-partum (288 samples). Program B sows had been vaccinated 3 weeks post-partum (276 samples). Program C sows had been vaccinated 3 times/year (290 samples). The means log2 CSFV antibody titer of the sows in programs A, B, C were 6.591.88, 5.722.04 …

Intra And Interspecies Somatic Cell Cloning In Rabbits, Cats And Goats, Mongkol Techakumphu, Pranee Numchaisrika, Ruttachak Rungsiwiwat, Ampika Thongpakdee, Malee Apimeteetumrong, Pramuan Virutamasen

The Thai Journal of Veterinary Medicine

Nuclear transfer from adult somatic cells is an advanced reproductive biotechnology. This technique can be applied in animal production for producing a high genetically identical copy for endangered species conservation and for therapeutic cloning for human medicine. Our three-year preliminary study indicates the possibility of somatic cell cloning by using the rabbit as model of technical development. Later, interspecies cloning in wild cats and domestic cats was performed successfully in terms of cloned embryo production. Meanwhile, goat cloning served as the model for farm animals.

The Use Of Porcine Small Intestinal Submucosa In Ten Cases Of Canine Corneal Staphyloma, Nalinee Tuntivanich, Kumpanart Soontornwipart, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

Porcine small intestinal submucosa biomaterial was used to repair corneal staphyloma in 10 dogs. There was no evidence of leakage of aqueous humor and graft rejection following PSIS grafting. Anterior synechia was presented in 25-50% of the corneal area. Granulation tissue vanished within 50 days of the operation. Even though scar formation and anterior synechia were observed at the end of the study, all dogs had vision.

Progesterone Receptors And Proliferation In The Bitch Uterus During Different Stages Of The Oestrous Cycle, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirivaidyapong

The Thai Journal of Veterinary Medicine

Immunopresence of the progesterone receptors (PR) and proliferative activity in the bitch uterus were studied during different stages of the oestrous cycle. Uterine samples were collected at 4 different stages of the oestrous cycle; prooestrus, oestrus, dioestrus and anoestrus. Immunohistochemistry was used to evaluate the localization and to semiquantitate PR and uterine proliferation by using monoclonal antibodies to the PR and the proliferative marker, Ki-67 respectively. The results showed that PR and Ki-67 levels were highest during prooestrus and oestrus while they were low at other stages. However, a significant difference was observed only in the surface epithelium for Ki-67 …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.

Observations On The Structures Of Red Blood Cells, White Blood Cells And Platelets In Some Felis Spp. And Panthera Spp., Wimon Pothiwong, Kamol Sakulwira, Pakorn Prachammuang, Siripen Vethchagarun

The Thai Journal of Veterinary Medicine

Blood cells and platelets from twenty-seven animals belonging to two genera of the Family Felidae, were studied using both light and transmission electron microscopes (TEM). Generally, blood cells can be categorized into red blood cells, white blood cells and platelets. The red blood cells or erythrocytes from these species were round and had neither a nucleus nor organelles. They showed a central pale area, Heinz bodies and rouleaux formation. The white blood cells could be divided into two groups; granulocytes and agranulocytes. The granulocytes (neutrophils, eosinophils and basophils) were spherical in shape with lobulated nuclei and containing specific granules in …