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Theses/Dissertations

2019

College of Natural and Health Sciences

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The Effects Of Fish Meal Supplementation On Gene Expression In The Bovine Corpus Luteum Following Low Dose Administration Of Prostaglandin F2a, Jessica Claire Cedillo Aug 2019

The Effects Of Fish Meal Supplementation On Gene Expression In The Bovine Corpus Luteum Following Low Dose Administration Of Prostaglandin F2a, Jessica Claire Cedillo

Master's Theses

The corpus luteum is a transient endocrine gland that develops from the remnants of the ovulatory follicle and secretes the steroid hormone progesterone. Progesterone is essential in the establishment and maintenance of pregnancy in all mammalian species. In the non-pregnant bovine, uterine prostaglandin F2α is secreted in a series of pulses between days 15 and 17 after ovulation, allowing the corpus luteum to undergo functional and structural regression and an opportunity for mating. During early pregnancy, trophoblastic cells of the developing embryonic placenta secrete interferon-tau, which attenuates uterine prostaglandin F2α secretion allowing for maintenance of the corpus luteum during the …


Challenges For Nursing Students In The Clinical Learning Environment, Huyen Thi Tran Aug 2019

Challenges For Nursing Students In The Clinical Learning Environment, Huyen Thi Tran

Master's Theses

The study aimed to survey the problems/challenges nursing students of Hong Bang International University (HIU) reported facing in the clinical environment. These factors might be a barrier or motivation for a student's clinical learning. In addition, collecting the information through this research helped explore the real difficulties that might exist when students practice clinical care. This data will help to develop appropriate recommendations to improve the quality of training as well as reduce the burden on nursing students. A descriptive cross-sectional design was used. Sixty-nine third-year nursing students (58 females and 11 males) of the Bachelor of Nursing Program from …


Comparison Of Socket Suspension Systems During Stair Ascent In Individuals With Transtibial Amputees, Gabrielle Marie Rentuma Aug 2019

Comparison Of Socket Suspension Systems During Stair Ascent In Individuals With Transtibial Amputees, Gabrielle Marie Rentuma

Master's Theses

Socket suspension systems have an important role in an amputee’s ability to perform activities of daily living. There are three common modes of suspension used to attach the prosthetic foot to the residual limb including lock and pin (PIN), suction, and vacuum suspension systems. A new vacuum suspension system has been developed, the PUCK, which has a vacuum system internal to the socket and maintains pressure throughout the residual limb. Previous literature has focused on the role of suspension during over-ground walking but few have examined the effects during a more difficult task such as stair ascent. The purpose of …


Effects Of Creatine Supplementation And Doxorubicin Treatment Of Myostatin And Muscle Ring-Finger Protein-1 Expression In The Rat Diaphragm, Addison M. Shepard Aug 2019

Effects Of Creatine Supplementation And Doxorubicin Treatment Of Myostatin And Muscle Ring-Finger Protein-1 Expression In The Rat Diaphragm, Addison M. Shepard

Master's Theses

Doxorubicin (DOX) is a highly potent chemotherapy drug that impacts the entire body rather than a targeted treatment area. While DOX treatment assists in the disruption and prevention of certain types of cancer growth, it damages many physiological processes in the body that were functioning normally and otherwise healthy prior to its administration. Damage induced by DOX on organ functioning can lead to other malignancies in the body, such as cachexia, cardiotoxicity, and myotoxicity, among others. Skeletal muscle relies on myogenic regulatory factors (MRFs), transcription factors expressed by satellite cells, for positive regulation of phases of muscle development and response …


Assessment Of Patient Waiting Consultation Time In An Primary Healthcare Clinic -- The Outpatient Department Of Cho Ray Hospital, Pham Thi My Nhung Aug 2019

Assessment Of Patient Waiting Consultation Time In An Primary Healthcare Clinic -- The Outpatient Department Of Cho Ray Hospital, Pham Thi My Nhung

Master's Theses

Long patient waiting times in primary healthcare clinics in South Vietnam such as Thong Nhat Hospital and An Giang Hospital are a common phenomenon. In South Vietnam, especially at the Cho Ray Hospital, long patient waiting times were to be expected. Unfortunately, very little knowledge exists regarding potential causes of this problem or how it impacts patients. The purposes of this non-experimental, exploratory field study were to (a) assess the process and outcomes of an outpatient clinic as they related to waiting times, factors contributing to waiting times, and associated factors (outcomes) that influenced patient satisfaction levels in the outpatient …


The Effects Of Exercise Training On Cachexia In Mice Bearing The Colon-26 Carcinoma, Nicole Wood May 2019

The Effects Of Exercise Training On Cachexia In Mice Bearing The Colon-26 Carcinoma, Nicole Wood

Dissertations

Colon cancer is third the most prevalent cancer in the United States in both men and women. However, it is also a largely preventable disease, as colorectal cancer (CRC) risk is increased by environmental factors such as poor diet and physical inactivity. Furthermore, CRC patients are highly susceptible to significant muscle wasting. Cachexia is classified as a multifactorial metabolic syndrome associated with impairments in immune function, fatigue, and overall weakness, that lead to increased morbidity and mortality. To date, multiple studies have shown that both aerobic and resistance training, individually, are highly effective in their ability to attenuate the deleterious …


Examining The Roles Of The Receptor Tyrosine Kinase Pvr And The Bone Morphogenic Protein Receptor Thickvein In Regulating Self-Renewal And Division Frequency In The Cyst Stem Cells Of The Drosophila Testis Niche, Nastaran Soleimanibarzi Mues May 2019

Examining The Roles Of The Receptor Tyrosine Kinase Pvr And The Bone Morphogenic Protein Receptor Thickvein In Regulating Self-Renewal And Division Frequency In The Cyst Stem Cells Of The Drosophila Testis Niche, Nastaran Soleimanibarzi Mues

Dissertations

Adult stem cells live in different tissues, and they support and regenerate both the tissue they reside in and themselves. The stemness behavior is tightly regulated by the niche. The Drosophila testis is a valuable model to study stem cells in their niche. In this niche, there are two populations of stem cells; germline stem cells (GSCs) and somatic cyst stem cells (CySCs). GSCs and CySCs provide the cellular structure required to maintain the production of sperm in Drosophila males. These stem cells co-mingle around a group of non-dividing somatic cells known as the hub, and is the niche that …


Together We Move: Exploring Belonging, Basic Psychological Need Satisfaction And Motivational Factors In A Socially Structured University Physical Activity Program, Jessica B. Kirby May 2019

Together We Move: Exploring Belonging, Basic Psychological Need Satisfaction And Motivational Factors In A Socially Structured University Physical Activity Program, Jessica B. Kirby

Dissertations

Emerging adulthood is a critical developmental life stage for individuals to establish healthy behaviors that enhance wellbeing, including quality relationships and physical activity (PA) engagement. Institutions of higher education are positioned to influence and support the development of these developmental tasks in emerging adults enrolled on their respective campuses. The purpose of this mixed methods study was to explore experiences of belonging, basic psychological need satisfaction (BNS) in PAbased social relationships, and motives to participate in a socially structured PA program among emerging adults on a university campus. Participants: University students who participated in the Fitness Buddies (FB) program (n=10), …


Comparison Of Automated Hearing Testing Approaches For Outpatients Receiving Ototoxic Chemotherapy, Ashley Stumpf May 2019

Comparison Of Automated Hearing Testing Approaches For Outpatients Receiving Ototoxic Chemotherapy, Ashley Stumpf

Capstones & Scholarly Projects

Detection of the highest audible frequency of hearing is used to monitor patients undergoing chemotherapy for ototoxic effects of pharmaceuticals. The current study evaluated the feasibility of utilizing Creare’s (2016) wireless attenuated hearing test system (WAHTS) in two outpatient cancer treatment centers to administer automated hearing tests for the identification of the highest audible frequency. Twenty cancer patients being treated with carboplatin and cisplatin were recruited for hearing testing and eight untrained nurses were recruited to operate the WAHTS. Ambient noise measurements were taken in each treatment center before and after hearing testing and supported the validity of threshold measurements. …


Extended High-Frequency Békésy Audiometry, Katie Ranshaw May 2019

Extended High-Frequency Békésy Audiometry, Katie Ranshaw

Capstones & Scholarly Projects

The purpose of this study was to provide extended high-frequency (EHF) data using two methods of automated Békésy audiometry performed on the MADSEN Astera 2 audiometer. Thirty-two adult participants were divided into 4 age groups: 18-30 (n = 10), 31-40 (n = 9), 41-50 (n = 4), and 51-65 years (n = 9). Participants were selected based on normal hearing (25 dB HL or better for the 250 Hz to 4,000 Hz frequency range) and absence of significant history for middle ear disorders, family hearing-related disease, excessive noise exposure, and use of ototoxic drugs. EHF hearing thresholds were delivered via …


Prevalence Of High-Frequency Notched Audiometric Configurations Among University Music Students And Faculty, Chandra Maas May 2019

Prevalence Of High-Frequency Notched Audiometric Configurations Among University Music Students And Faculty, Chandra Maas

Capstones & Scholarly Projects

The present study determines the prevalence of audiometric notched configurations suggestive of noise-induced hearing loss (NIHL) among students and faculty at the University of Northern Colorado (UNC) School of Music. The prevalence of NIHL was determined by analyzing the results of 233 hearing evaluations that were administered during Fall semester music student orientation. High-frequency notches were characterized by evaluating the hearing thresholds at 3, 4, or 6 kHz being 10 dB or worse than the thresholds at 2 and 8 kHz, as defined by Wilson and McArdle, (2013). The prevalence of high-frequency audiometric notches was examined in terms of notch …


Can Performance On A Speech-In-Quiet Monosyllabic Word List Predict Speech-In-Noise Capabilities?, Ellyn Mary Kuehne May 2019

Can Performance On A Speech-In-Quiet Monosyllabic Word List Predict Speech-In-Noise Capabilities?, Ellyn Mary Kuehne

Capstones & Scholarly Projects

The purpose of this capstone research project was to determine if the Hurley and Sells (2003) speech-in-quiet test NU-6 10- and 25-word lists could be used as predictors for speech-in-noise (SIN) abilities gauged by the quick speech-in-noise test (QuickSIN; Etymotic Research Inc., 2001) or Bamford-Kowal-Bench speech-in-noise test (BKB-SIN; Etymotic Research Inc., 2005). Most audiologists use monosyllabic speech-in-quiet word lists to predict speech recognition abilities and hearing aid candidacy (Lindley, 2015). By not evaluating speech in noise, many hearing aid users are fit with hearing aids and counseled incorrectly (Mueller, Ricketts, & Bentler, 2014). If a modified monosyllabic speech-in-quiet word list …


Cervical Cancer Screening Management In Primary Care: A Quality Improvement Project, Amanda Lynn Miller May 2019

Cervical Cancer Screening Management In Primary Care: A Quality Improvement Project, Amanda Lynn Miller

Capstones & Scholarly Projects

Cervical cancer screening has evolved throughout the years into the current, very effective, algorithms for screening and management. The success of improved early detection of cervical cancer has saved many lives (Lees, Erickson, & Huh, 2016). The addition of human papillomavirus testing and genotyping has allowed for more efficient, and less invasive, management of cervical cancer screening (Cox, 2009). While there are significant advantages to these new guidelines, there are barriers to applying them in practice. The clinical site for the project was identified to be in need of a quality improvement project aimed at creating an improved patient notification, …


Human Immunodeficiency Virus Screening: Knowledge, Attitudes, Perceived Norms, And Control Beliefs Of Advanced Practice Registered Nurses In Colorado, Jennifer Berry May 2019

Human Immunodeficiency Virus Screening: Knowledge, Attitudes, Perceived Norms, And Control Beliefs Of Advanced Practice Registered Nurses In Colorado, Jennifer Berry

Capstones & Scholarly Projects

The purpose of this evidence-based practice project was to increase the knowledge base to address the barriers preventing routine screening of the human immunodeficiency virus (HIV). The project consisted of a statewide survey to assess the knowledge, attitudes, perceived norms, control beliefs, barriers, and facilitators of advanced practice registered nurses (APRNs) across Colorado. A statewide assessment is important in the state of Colorado because APRNs are the primary providers in many rural areas. There were 66 responses to the survey. Knowledge of the Centers for Disease Control and Prevention (CDC; 2018) guideline for HIV screening was lacking. About one-third of …


Diabetes Education Referral From The Emergency Department To Improve Patient Care And Health Literacy, Chelsea D. Lovelace May 2019

Diabetes Education Referral From The Emergency Department To Improve Patient Care And Health Literacy, Chelsea D. Lovelace

Capstones & Scholarly Projects

Diabetes is a complex disease that can lead to increased morbidity and mortality. Inadequate disease management is considered when patients present to the emergency department (ED) for care related to this disease. Once out of the hospital or ED, it is imperative the patient receives timely follow-up care to effectively manage the disease to reduce morbidity and mortality and to decrease return visits to the ED. Primary care across the nation is facing a national shortage with provider time spent with patients decreasing and patients’ length of time spent waiting to get an appointment increasing (Medscape, 2017; Merritt Hawkins, 2017 …


Waveform Characteristics Of The Canine Click Evoked Brainstem Auditory Evoked Response Across Multiple Test Sessions, Hayden H. Bruce May 2019

Waveform Characteristics Of The Canine Click Evoked Brainstem Auditory Evoked Response Across Multiple Test Sessions, Hayden H. Bruce

Capstones & Scholarly Projects

The purpose of this capstone research project was to investigate the consistency of waveform characteristics of the canine click evoked brainstem auditory evoked response (BAER) across multiple test sessions. Six canines were recruited to participate in the study. Brainstem auditory evoked response recordings were measured in each ear of the canines using a click stimulus at 102 dB peSPL, 82 dB peSPL and 62 dB peSPL. Canines included in the study were between the ages of one and seven to avoid both maturation and aging affects. Characteristics assessed throughout this study at each intensity level were absolute latencies of waves …


Acute Care Nurses’ Attitudes Toward Nursing Students With Disabilities, Kristy Lynn Calloway May 2019

Acute Care Nurses’ Attitudes Toward Nursing Students With Disabilities, Kristy Lynn Calloway

Dissertations

The purpose of this study was to describe acute care nurses’ attitudes toward nursing students with disabilities, how these attitudes were impacted by the acute care culture, and what significance they had for behavior. No studies have been done within the United States in relation to attitudes acute care nurses hold toward nursing students with disabilities practicing and learning in their clinical settings. In this focused ethnography, semi-structured interviews were conducted with 20 acute care nurses who had at least two years’ experience precepting nursing students. While exploring acute care nurses’ attitudes toward nursing students with disabilities, six themes emerged: …


Developing Support Mechanisms For Comprehensive School Physical Activity Program Leaders, Peter Teake Stoepker May 2019

Developing Support Mechanisms For Comprehensive School Physical Activity Program Leaders, Peter Teake Stoepker

Dissertations

In this dissertation, support mechanisms for comprehensive school physical activity program (CSPAP) leaders were investigated. In the first study, a systematic review was completed that explored the effectiveness of CSPAP professional development/training. In total, 8,982 records were screened and two articles matched the eligibility criteria and were included within the review. One study examined effectiveness using qualitative methods (Centeio, Erwin, & Castelli, 2014) and the other using quantitative methods (Carson, Castelli, Pulling Kugh, et al., 2014). Due to the limited number of articles that met the search criteria, it can be concluded that there is limited evidence to fully understand …


Effectiveness Of Simulation In Addressing Stigma, Sundeep Kaur Varaich May 2019

Effectiveness Of Simulation In Addressing Stigma, Sundeep Kaur Varaich

Dissertations

Mental health stigma hinders quality nursing care. The aim of this quasi-experimental study was to test if simulation was effective for addressing stigma in nursing education and evaluating student attitudes towards psychiatric conditions. A sample of eight-nine undergraduate nursing students were assigned to a control or treatment group and participated in either a chronic health challenge scenario or a mental health scenario to test the effectiveness of using a mental health simulation to address stigmatizing attitudes. Day’s Mental Illness Stigma Scale was used as the data collection tool for the post-test to measure students’ stigmatizing perceptions in relation to their …


Measuring Impact Noise With Smartphone Apps, Jacob Page Leons May 2019

Measuring Impact Noise With Smartphone Apps, Jacob Page Leons

Capstones & Scholarly Projects

The ability of smartphone apps to measure impact noise has not been evaluated. This study was designed to explore the feasibility of using smartphone apps as a means to evaluate impact noise levels in industrial settings. Impact noise was generated by dropping a 4 Kg shotput onto a .5” thick steel plate at heights ranging from 6.5 to 102 cm. Two iPhones and two Android phones were tested with three apps each using both the phone’s built-in microphone and an external microphone. Sound level measurements of each drop were simultaneously recorded by a calibrated smartphone and a gold standard system …


Recognizing Possible Schizophrenia In The Primary Care Setting: A Brief Information Sheet, Susan H. Winchester May 2019

Recognizing Possible Schizophrenia In The Primary Care Setting: A Brief Information Sheet, Susan H. Winchester

Capstones & Scholarly Projects

Schizophrenia occurs in about 1.1% of the population in the United States--more than 2.2 million. Over 100,000 patients will be diagnosed with schizophrenia in the United States this year. As of 2016, there is no cure for schizophrenia but the treatment success is high (over 50% completely recover or are much improved with relative independence). The cost of schizophrenia is estimated to be over $6.2 billion. Most people with schizophrenia are seen by a primary care provider before they receive referral to a mental health professional. In fact, primary care visits are six times higher in the six years before …


Current Perspectives On The Gestalt Of Nursing Curricula, Brandi Lynne Venvertloh May 2019

Current Perspectives On The Gestalt Of Nursing Curricula, Brandi Lynne Venvertloh

Dissertations

This research study aimed to investigate the current gestalt of the Bachelor of Science in Nursing (BSN) curricula in the United States. Presently there is an emphasis in nursing education on the practice–education gap in order to ensure new graduate Registered Nurses are adequately prepared for entry into practice in a dynamic and complex healthcare environment. There has also been movement in nursing education to remove nursing theory from BSN curriculum guiding frameworks and replace them with essential educational standards. The purpose of this qualitative phenomenological research study was to explore the lived experiences and perspectives of faculty curriculum leaders …


Blooming Where One Is Planted: Understanding Flourishing Among Rural Nurses, Marnee Lynne Crawford May 2019

Blooming Where One Is Planted: Understanding Flourishing Among Rural Nurses, Marnee Lynne Crawford

Dissertations

The reality of the challenges experienced in rural hospital-based care leave sparsely populated areas to face critical losses in nurses and healthcare services within already underserved regions of the nation. Nursing education is uniquely poised to serve as a mitigating agent against shortages in rural nursing workforce by preparing rural graduates to bloom where they are planted. This qualitative interpretive phenomenological research study explored the meaning of human flourishing as it is lived among rural nurses in the northwest region of Wyoming. The research was framed using social constructivism and the lens of rural nursing theory. Data were collected and …


The Lived Experience Of Underrepresented Pre-Nursing Students: An Exploration To Diversify The Nursing Workforce, Katie Bagley May 2019

The Lived Experience Of Underrepresented Pre-Nursing Students: An Exploration To Diversify The Nursing Workforce, Katie Bagley

Dissertations

Understanding about the experience of underrepresented students making the decision to choose nursing as a career would improve the ability of nursing programs to recruit applicants from a more diverse background. The purpose of this research was to explore the experience of underrepresented students who are considering nursing as a career but have not yet applied to a nursing program. This research was conducted using a descriptive phenomenological research method. Thirteen participants participated in the study. The participant interviews were interpreted as an expression of pre-nursing students from racially or ethnically underrepresented backgrounds who are feeling they are in a …


Magnetic Minerals Of The Lower Poleslide Member Of The Brule Formation, Badlands National Park, Rebecca V. Kipf May 2019

Magnetic Minerals Of The Lower Poleslide Member Of The Brule Formation, Badlands National Park, Rebecca V. Kipf

Master's Theses

The White River Group in Badlands National Park is significant because it contains abundant mammal fossils. Many of these fossils are unique to the Great Plains and one of the major challenges has been to learn where these fossils fit in the geologic global time scale. Paleomagnetic studies have been instrumental in correlating and dating these faunas. The purpose of this study is to look at the specific magnetic mineralogy for a complete stratigraphic column of the lower Poleslide Member of the Brule Formation at Cedar Pass. This study attempts to determine what minerals provide the paleomagnetic remanence in these …


The Impact Of Shod Versus Unshod Walking On Center Of Pressure Variability, Zachary Barrons May 2019

The Impact Of Shod Versus Unshod Walking On Center Of Pressure Variability, Zachary Barrons

Master's Theses

The purpose of the present investigation was to examine the influence of footwear on walking stability. Twenty healthy women walked at 1.3 m/s on an instrumented treadmill. One hundred steps (50-right, 50-left) were analyzed from two walking conditions (shod, unshod). The variability of the center of pressure (COP) for each step was calculated for quadrants of the contact period. Significant differences in variability were seen between shod and unshod conditions in all quadrants as well as differences between left and right feet in quadrants one and four. Restricted foot motion while shod may explain the differences seen in COP variability. …


Effects Of Exercise Training On The Blood Lactate Response To Acute Exercise In Cancer Survivors, Arjun Ramani May 2019

Effects Of Exercise Training On The Blood Lactate Response To Acute Exercise In Cancer Survivors, Arjun Ramani

Master's Theses

Otto Warburg, a German physiologist, deduced that cancer cells exhibit a remarkably different metabolic profile in comparison to normal healthy cells characterized by increased rates of glycolysis and lactate fermentation, even in the presence of adequate oxygen content. Through the respective gain of function/loss of function of oncogenes and tumor suppressor genes, three major genes emerge as hallmarks of carcinogenic metabolic reconfiguration; HIF-1a, c-Myc oncogenes, and p53 tumor-suppressor genes. A result of their respective gain/loss of function are the upregulation of several glycolytic proteins and subsequent high rates of glucose influx into the tumor microenvironment, as well as lactate production, …


Application Of The Wireless Audiometric Testing System At A Refugee Center With A Multilingual Population, Jennifer Ruths May 2019

Application Of The Wireless Audiometric Testing System At A Refugee Center With A Multilingual Population, Jennifer Ruths

Capstones & Scholarly Projects

The purpose of this study was to assess the state of hearing health in the immigrant and refugee community of northern Colorado, while also determining if the new wireless automated hearing test system (WAHTS) technology is a feasible means of bringing entry-level care to this multilingual population. Twenty adult participants were recruited from various cultural/lingual groups including: Rohingya, Karenni, Spanish, and Somali. Data were analyzed for 19 participants, 57.9% of who were female and 42.1% of who were male. Mean age of participants was 52.3 years (SD= 16.05). Audiometric thresholds were obtained at .5 to 8 kHz in both ears …


A Multifaceted Approach To Address Variation In Cannabis Sativa, Anna Louise Schwabe May 2019

A Multifaceted Approach To Address Variation In Cannabis Sativa, Anna Louise Schwabe

Dissertations

For thousands of years, humans have cultivated and dispersed Cannabis sativa L. across the globe. Although Cannabis has been largely illegal worldwide for decades, public perceptions and attitudes are changing. Increasing interest in potential Cannabis usage worldwide and nationwide is leading to less restrictions to make way for an expanding and lucrative industry with numerous applications. Although only one species is formally recognized in the Cannabis genus, thousands of years of artificial selection for diverse phenotypes and uses have resulted in two major usage groups; hemp-types which are defined worldwide as having very low levels of THC (< 1.0%), and drug-types which exceed a specified level of THC that varies among nations. The drug-type category includes three commonly used subcategories including Sativa, Indica and Hybrid types, and newly developed high CBD varieties that have more THC than hemp-types but are not bred for high THC. The quality of federally produced Cannabis for medical studies in the U.S. has recently been brought into question, and we included samples to determine the genetic relationship to these groups. Phenotypic variation in Cannabis gives rise to commonly referenced categories, but sources of variation are unclear and understudied. Phenotypes are observable characteristics that results from a combination of both genotype and the environment. The preferred method of propagation for Cannabis is cloning, and therefore variation within varietals should be from differences in environmental factors. Ten microsatellite markers were developed de-novo to investigate four aims: (1) genetic variation within strains, (2) genetic relationships among the common categories, (3) if genetic variation is detectable through olfactory sensation, and (4) how genetic variation is reflected in phytochemical levels. This dissertation includes four manuscript chapters representing each aim and uses a genetic basis for a multifaceted approach to investigate variation in Cannabis sativa. Substantial genetic variation was found within strains from obtained from different facilities. Genetic divergence between hemp and drug-types was genetically supported, but the Sativa, Indica, and Hybrid subcategories were not genetically well defined. The high CBD strains appear to bridge the genetic gap between hemp and drug-types, and federally grown research grade marijuana was genetically more similar to hemp than Cannabis available through the legal cannabis market. Genetic imposters within a strain had measurable aromatic differences, but there was considerable variation in aromas among samples with identical genetic identity. Analyses of both terpene and cannabinoid profiles among individuals with identical genotypes acquired from different sources varied considerably indicating environmental variation has a substantial impact on phenotype in Cannabis. Together these results show a need for the Cannabis industry to implement regulatory checks in the form of genetic testing in order to provide consistency, especially for medical applications. These results demonstrate the need for genotyping in order for phenotypic consistency to be achieved if standard growing conditions can be established. When genetic verification and standard protocols are established, deviations in phenotypic changes can be identified and disclosed to consumers so they are aware that there may be abnormal effects. This investigation highlights the need for additional research to provide consistent products, which is especially important for medical marijuana flower products. In order to provide consumers consistent products, it is imperative to understand sources of variation. Consumers deserve to be provided with quality consistent products as the industry continues to grow on a global scale.