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Identification Of Micrornas In Bovine Spermatozoa With Implications Of Fertility, Lashonda S (Lashonda Shakita) Robertson Dec 2009

Identification Of Micrornas In Bovine Spermatozoa With Implications Of Fertility, Lashonda S (Lashonda Shakita) Robertson

Theses and Dissertations

MicroRNAs are small RNA molecules that could possibly play a major role in fertility. In the experiment, spermatozoa were extracted from bovine followed by an extraction of total RNA. Bovine spermatozoa were extracted from two bulls of different fertility, high and low fertility. An expression array was done to compare the expression levels of the microRNAs. It was shown that thousands of microRNAs are present in bovine spermatozoa but only a small amount was significantly expressed. The microRNAs from low fertility bulls were more highly expressed than those in high fertility bulls. A Bioanalyzer gel was used to confirm the …


Not Trying: Reconceiving The Motherhood Mandate, Kristin J. Wilson Dec 2009

Not Trying: Reconceiving The Motherhood Mandate, Kristin J. Wilson

Sociology Dissertations

Infertile and childless women think about, live with, and defend their status as mothers and as nonmothers, arguably more so than other women for whom motherhood comes about accidentally or relatively easily in accordance with a plan. Within this group of infertile and childless women are those who are otherwise socially marginalized by factors like class, race, age, marital status, and sexual identity. This dissertation asks about the ways in which marginalized infertile and childless women in America make sense of their situations given the climate of “stratified reproduction” in which the motherhood mandate excludes them or applies to them …


The Effect Of Moderate Exercise On Folliculogenesis, Cortisol, Estradiol And Luteinizing Hormone In Mares, Dale Kelley Dec 2009

The Effect Of Moderate Exercise On Folliculogenesis, Cortisol, Estradiol And Luteinizing Hormone In Mares, Dale Kelley

All Theses

Assisted reproductive technologies allow mares to remain in training and produce offspring; however, limited data regarding the impact of exercise on folliculogenesis exists. Mares in training are reported to have reduced embryo recovery rates and ovulated smaller follicles. The objectives of this study are to evaluate the effect of exercise on folliculogenesis using ultrasound technology. Additionally, cortisol, luteinizing hormone (LH), follicle stimulating hormone (FSH) and estradiol concentrations in mares were evaluated. The exercise period was from April to September 2008 at Latitude: 34¡ 41' 1' N. Thirteen horses were assigned to Exercise (n=6) or Control group (n=7). Mares were exercised …


Changing Fecundity And Reproductive Output In Females Of A Chesapeake Bay Population Of Blue Crab, Callinectes Sapidus, Shannon L. Wells Jul 2009

Changing Fecundity And Reproductive Output In Females Of A Chesapeake Bay Population Of Blue Crab, Callinectes Sapidus, Shannon L. Wells

OES Theses and Dissertations

The Chesapeake Bay blue crab, Callinectes sapidus, is an important species both ecologically and as a fishery. Fishery dependent and independent data indicate that the population declined abruptly in the mid-1990s and has remained low. Previous work prior to the decline in population abundance found a significant relationship between size and fecundity for mature females. As with many heavily fished populations, this population has experienced a reduction in the size at maturity. The objectives of this study were to reassess the fecundity of the population, determine the lipid and protein energy allocated for reproduction, and to examine the concentrations …


The Effects Of Feeding A Ground Raw Soybean Diet On Reproductive Performance And Carcass Quality Of Prepubertal Gilts, Daniel Jason Sykes May 2009

The Effects Of Feeding A Ground Raw Soybean Diet On Reproductive Performance And Carcass Quality Of Prepubertal Gilts, Daniel Jason Sykes

Theses and Dissertations

The administration of raw soybeans to the diet of prepubertal gilts had no effect (p>0.05) on age to puberty, number of corpora lutea, or embryos present. Also, the number of pigs farrowed live and 28d litter weights did not differ between gilts consuming the raw soybean diet and gilts consuming the control diet. There was a reduction (p<0.05) in the number of pigs weaned in the group consuming the raw soybeans but no differences (p>0.05) in weaning weights. Gilts fed the raw soybean diet had a reduction (p=0.05) of hot carcass weights compared to gilts consuming the control diet but exhibited no differences for other carcass parameters measured. Thermal imaging was able to differentiate gilts …


Management Strategies To Improve Development Of Replacement Heifers Utilizing Tall Fescue-Based Systems, Margaret Miller May 2009

Management Strategies To Improve Development Of Replacement Heifers Utilizing Tall Fescue-Based Systems, Margaret Miller

All Theses

Research was conducted to determine the effect of tall fescue and alternative management regimens on reproduction in replacement beef heifers. Angus _ Simmental heifers (n = 48; 318 ± 4.99 kg initial BW) stratified by weight and pubertal status were assigned to one of three replicated grazing treatments: (1) toxic endophyte-infected tall fescue (TF); (2) toxic endophyte-infected tall fescue with a soybean oil supplement (TFO); or (3) mixed winter annual species (cereal rye, annual ryegrass, crimson clover and hybrid turnip) (M). Tall fescue endophyte infection level was between 74 and 94%. Pastures were grazed from December 12 to April 24. …


Influences Of Supplementing A Mannan Oligosaccharide Containing Product To Pig Diets On Sow And Weanling Pig Performance, I-Fen Hung Jan 2009

Influences Of Supplementing A Mannan Oligosaccharide Containing Product To Pig Diets On Sow And Weanling Pig Performance, I-Fen Hung

University of Kentucky Master's Theses

The objective of these studies was to evaluate the effect of supplying mannan oligosaccharides (MOS) to pig diets on both weanling pig and sow performances.

Nursery pigs challenged with LPS to stimulate an immune challenge had increased body temperature and respiratory rate as well as elevated serum cortisol and TNF-α concentration, MOS-supplemented pigs had a lower rectal temperature and respiratory rate which implied that MOS improves some aspect of the immune function of piglets.

MOS supplementation in sow diets during late gestation and lactation had no effect on litter size, but the piglets from MOS-fed sows were heavier at birth …


Age, Growth And Reproduction Of The Bering Skate, Bathyraja Interrupta (Gill & Townsend, 1897), From The Eastern Bering Sea And Gulf Of Mexico, Shaara Michiko Ainsley Jan 2009

Age, Growth And Reproduction Of The Bering Skate, Bathyraja Interrupta (Gill & Townsend, 1897), From The Eastern Bering Sea And Gulf Of Mexico, Shaara Michiko Ainsley

Capstone Projects and Master's Theses

The Bering skate, Bathyraja interrupta, is one of the most common skate species in Alaskan waters. Skates are a regular bycatch in Alaskan commercial long line and bottom trawl fisheries, and there is increasing interest in directed fishing of skates in the region. Until recently, little was known about the life histories of Alaskan species. This is the first study to examine the age, growth and age at maturity of B. interrupta, and one of the few studies to compare life history characteristics of a skate species among management regions. No significant difference between sexes was detected in either region. …


Proactive Procreation : Ethical Implications Of New Genetic Technologies For Parental Obligations To Future Offspring, L Syd M. Johnson Jan 2009

Proactive Procreation : Ethical Implications Of New Genetic Technologies For Parental Obligations To Future Offspring, L Syd M. Johnson

Legacy Theses & Dissertations (2009 - 2024)

This dissertation examines the ethical implications of recent genetic innovations, particularly preimplantation genetic diagnosis, for the obligations and duties of parents to future children. I critique common but uncompelling objections to the use of genetic information, diagnosis, and technology to influence the characteristics of future offspring, and conclude that genetic selection and enhancement are not different in kind or degree from other means of selection, direction, and enhancement that parents engage in to shape the lives of their children. Procreation is morally risky -- it risks imposing substantial burdens on persons who would not otherwise have to bear those burdens, …


The Role Of A Spinothalamic Pathway Regulating Sexual Inhibition And Reward, Allisha M. Bailey Jan 2009

The Role Of A Spinothalamic Pathway Regulating Sexual Inhibition And Reward, Allisha M. Bailey

Digitized Theses

Male sexual behaviour is a rewarding behaviour with ejaculation being the most reinforcing component. However, the neural pathways that process and relay ejaculation-specific sensory information and mediate sexual reward are currently unknown. A candidate spinothalamic pathway has recently been discovered to be in the position to process and relay ejaculatory-related cues from the reproductive organs to the brain. This pathway consists of a population of lumbar spinothalamic cells projecting to an area of the thalamus, known as the medial parvocellular subparafascicular thalamic nucleus, and expresses several neuropeptides, including galanin and cholecystokinin. The current study demonstrates that this spinothalamic pathway and …