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The Effects Of Ammonia On The Functionality Of Bovine Granulosa Cells, Micah Henrie

All Graduate Theses and Dissertations, Fall 2023 to Present

It is common practice in the livestock animal production industries to feed high levels of protein to maximize the yield of each animal in production. However, diets high in protein can cause decreased reproductive success within these animals. The causes behind this decrease are not fully understood. Increased dietary protein leads to increased levels of both ammonia and urea in the blood of the animal. These increased metabolite levels may be a possible explanation for the decline in reproductive success. The goal of this study is to identify how various levels of ammonia affect the function of bovine granulosa cells …

Conservation And Management Of Greater Sage-Grouse In Strawberry Valley: Quantifying Influences On A Traditional Capture Method And Long-Term Trends In Clutch Size, Janae Radke

Theses and Dissertations

The greater sage-grouse (Centrocercus urophasianus) is a species of conservation concern that has undergone serious declines in the last century. The Strawberry Valley sage grouse population in Utah underwent such a decline from an estimated 3,500 sage grouse in the 1930s to 150 sage grouse in the early 2000s. This decline initiated a long-term conservation and monitoring project in Strawberry Valley with the goal of preserving the sage grouse population. As part of this ongoing conservation project, we investigated factors that impact the success of capturing sage grouse using the night-lighting method (Chapter 1). We found that capture success is …

Inclusivity Of In Vitro Fertilization Clinic Websites, Erin Donahue

Honors Theses and Capstones

In the world, many people cannot naturally conceive children. They may be infertile, lack an intimate partner, or be in a same-sex relationship. Artificial reproductive technologies (ARTs) are catered to the aforementioned population. ARTs are fertility treatments that assist people with the fertilization of gametes and/or implantation of embryos. One of the most popular ARTs is in-vitro fertilization (IVF). IVF is a medical procedure in which an egg is fertilized by sperm in a lab so it can develop into an embryo. That embryo can then be implanted directly into the uterus. This quality improvement project aims to determine the …

A More Modern Prometheus: What Frankenstein Tells Us About Genetic Modification, Allison M. Ambrose

Honors College Theses

Mary Shelley’s famous novel, Frankenstein, is often hailed as the first true science fiction novel. In my thesis, I use the premonitive lens towards creation of life provided in Frankenstein to evaluate the morality of genetic modification of children. CRISPR-Cas9 is quickly emerging as the most important development in reprogenetic technology of our time, and many argue for its merits as a method of designing our children. I argue against this trend of “designer babies,” specifically raising questions about the soundness of modifying non-disease traits in future children and encouraging a more cautious attitude in both the scientific and philosophical …

Expression Of Growth Differentiation Factor 15 (Gdf-15) In Placental Tissue Of Full- Term Pregnant Women With Hypertensive Disorders In A Western Australian Population, Elsa H. L. Chew

Theses: Doctorates and Masters

Women who experience hypertensive pregnancy disorders (HPDs) during pregnancy, mainly pre-eclampsia (PE), are four times more likely to develop hypertension after pregnancy and therefore have an increased risk of developing cardiovascular diseases (CVD) in the future. Pregnancy involves a high level of cardiovascular stress and the development of some complications. This has the potential to increase a woman’s susceptibility to vascular or metabolic disease risk in the future. In this research, pre-eclampsia (PE) and pregnancyinduced hypertension (PIH) were representatives of HPDs and were analysed together for the expression of Growth Differentiation Factor-15 (GDF-15) relative to their healthy agematched controls.

GDF-15 …

Spawning Asynchrony Of The Endangered Acropora Cervicornis: Are Light Pollution And Abnormally Warm Temperatures The Culprits?, Krista Laforest

All HCAS Student Capstones, Theses, and Dissertations

The persistence of reefs relies on mature corals spawning synchronously to maximize fertilization and produce larvae to replenish local populations. Corals synchronize the release of gametes by responding to temperature, sun, and moon light cycles; however, abnormalities in these patterns can disrupt synchrony. This study is the first to describe regional asynchronous spawning of Acropora cervicornis by quantifying gamete development and spawning times among two reefs, an in situ nursery off Fort Lauderdale, and an in situ nursery in the Florida Keys. While A. cervicornis in the Florida Keys synchronously spawned within the predicted window of 2-5 days after the …

Impact Of Cadmium Stress On Soil Virus Reproduction And The Persistence Of Viruses Under Abiotic Conditions, Zhibo Cheng

Doctoral Dissertations

Soil viruses are ubiquitous and greatly impact the structure and function of soil microbial communities, with their effects modulated by various environmental factors. This study investigates the inactivation of naturally occurring soil viruses in sterilized soil, as well as the effects of cadmium (Cd) exposure and carbon (C) and nitrogen (N) nutrient availability on the population dynamics of virus-host communities in agricultural soil.

Lab-scale slurry and unsaturated experiments were performed to examine virus inactivation in the absence of host bacteria in sterilized soil. In slurry experiments, virus abundance declined by over 90% after 10 days of incubation. The addition of …

Assessing The Effects Of The Spring Hunting Season Start Date On Wild Turkey Seasonal Productivity And Hunter Behavior, Joseph Quehl

Masters Theses

Many states throughout the Southeast have documented declines in wild turkey (Meleagris gallopavo) harvest and estimates of recruitment in poult-per-hen ratios. Wild turkey populations are driven by seasonal productivity, so the decline in these parameters may indicate a decline in the overall population. One hypothesis as to why we are seeing a reduction in productivity and a potential population decline is that the spring hunting season is disrupting the reproductive cycle by harvesting too many males before they have had the opportunity to breed, or by harvesting dominant males and disrupting the social hierarchy of the flock. Our …

Evaluating The Role Of The Bovine Vaginal Microbiome In Neonatal And Maternal Health Outcomes, Riley D. Messman

Theses and Dissertations

The dam vaginal microbiota is the first major microbial inoculating community within the neonate. The composition of the dam vaginal microbiota has implications in calf commensal microbiota development. Alterations of the dam microbial community prior to parturition could alter inoculating communities and immune responses in both the dam and calf. Thus, authors aimed to elucidate the microbial community composition of the bovine dam vaginal and calf nasal microbiota post-partum after utilizing betadine lavages (BL). The dam vaginal and calf nasal microbial communities and immune responses were evaluated at 0-, 15-, 30- and 60-day post-partum. Microbiota composition of the dam haircoat, …

Unruly Periods: Reproductive Futurities And The Rhetorics Of Menstruation, Hannah Taylor

All Dissertations

“Unruly Periods: Reproductive Temporalities and the Rhetorics of Menstruations” argues that dominant rhetorics of shame and regulation around menstruation work to maintain strict reproductive temporalities that uphold heteropatriarchal norms. Specifically, I draw upon scholarship in queer studies and disability rhetorics to assert that sexual health texts (such as puberty books), menstrual care products (pads and tampons), and technologies of menstruation (period-tracking apps) function as a form of chronobiolitics—a teleological force that seeks to reinforce bodily normalcy. In doing so, these rhetorics of menstruation deny or elide the embodied experiences of diverse, queer, and disabled menstruators, limiting reproductive possibilities. Reproductive justice …

Embodying Abortion: Approaches To Sexual And Reproductive Experiences Of The Cairene Youth., Ruth Morales Cosano

Theses and Dissertations

Whilst abortion in Egypt has been sorely neglected in academic literature, and highly criminalized by state institutions, women in Egypt have by no means ceased to perform it and, what is more, upon reasons that exceed any institutional or legal reasoning. The thesis stems from the need to give voice and continuity to the experiences of women who abort in Cairo. Given the almost complete lack of material showcasing this reality, this research project investigates the meaning-making process of abortion among Cairo's middle and upper classes. Understanding this construction requires studying abortion in its embeddedness in the material, social, and …

Growing Brine Shrimp (Artemia) In Desalination Brine Water, Khaled Madkour

Theses and Dissertations

Water scarcity is one of the main issues in the recent and coming decades that resulted from the growing population, increasing agricultural lands, and other current challenges. This problem led to finding alternatives to provide fresh water for domestic usage. Desalination is one of these solutions, especially in the MENA region, which has more than half of the desalination plants in the world. The desalination process has two main outputs: freshwater and highly concentrated saline water (brine water). The brine water discharge can be done in various approaches, including deep-well injection, surface water disposal, land application, effluent disposal, and evaporation …

Bi Erasure And Bi Invisibility In Media And Medicine: Moving Beyond, Lacy M. Telles

Dissertations, Theses, and Capstone Projects

This project examines the bisexual erasure and invisibility that is found in film, television, literature, and in the field of reproduction. Even though bisexual people make up a large portion of the LGBTQ community, because of bi invisibility, they tend to get lost in the cause. This website offers information about bi invisibility, highlights obstacles faced by bisexuals, provides resources for bisexuals, and includes some fun topics for bisexual people. It also has a page of bisexual testimonies: real bi people with real bi stories. There is evidence of erasure and invisibility, especially in children’s books and the field of …

An Examination Of The Vaginal And Endometrial Microbiota Of Heifers And The Effects Of Selenium Supplementation On Microbiome Composition, Kobe S. Perry

Honors Theses

Selenium, an essential trace element, is involved in many biological processes serving catalytic, structural, and regulatory roles through mechanisms that are not entirely understood. Due to its health effects from either deficiency or toxicity, selenium has been well-studied, with an emphasis towards selenium deficiency which has been shown to cause immune and reproductive issues in animals. To address deficiency, different forms of supplementation have been used, yet few studies have examined selenium’s effects on the reproductive microbiome based on the type of supplementation (i.e., inorganic or organic forms). By examining bovine vaginal and endometrial mucosal samples via 16S gene sequencing, …

Parental Instincts: The Neurological And Biological Factors Associated With Parenthood, Jared Reeder

Undergraduate Honors Theses

The following project involves a systematic review of the scientific literature on neural and biological changes of mothers and fathers in parenthood. Until very recently, little scientific research was devoted to studying how bearing children affects a man or woman’s long-term biology. Over the last twenty years, studies of neuroplastic changes in new mothers show specific neural mechanisms responsible for altering the behaviors of mothers during and after pregnancy. These changes in neuroplasticity alter behavior in such a way that led to mothers requiring less sleep and being more prone to hearing the cries of their children. In addition to …

Detection And Observed Correlations Of Statin And Beta-Blocking Pharmaceuticals In West Virginia Surface Waters And Ichthyofauna, Joseph W. Kingsbury

Graduate Theses, Dissertations, and Problem Reports

Within this thesis the source, presence, and the estimated ecotoxicological effects of cardiovascular medicines, specifically statins and beta-blockers are described within the Buckhannon, Tygart Valley, and West Fork Rivers of West Virginia. Included within this thesis is a general introduction providing key background information and relevant research related to the source, presence, and known toxicity of statins and beta-blockers. Following the general introduction there are 3 distinct chapters. Chapter 1 focuses on the estimated demographic based source loadings and the surface water concentration characteristics of statins and beta-blockers within the three rivers. Chapter 2 investigates the effects of statins and …

Quantifying Life History Energetics Of An Oviparous Elasmobranch Subject To Future Warming Water, Carolyn R. Wheeler

Graduate Doctoral Dissertations

Marine ectothermic fishes – fishes that do not regulate internal body temperature independent from the water temperature – are some of the most vulnerable organisms to rapid and alarming increases in environmental temperature occurring due to climate change mediated-ocean warming. For tropical fish species that live near their thermal maxima, this warming could be of grave consequence to individuals, populations, and whole marine ecosystems. Certain key life stages such as embryos and reproducing adults may be particularly vulnerable to warming given the excess energetic costs of these life stages, which may disallow compensation for extreme changes in temperature. One taxonomic …

Seeing Children’S Futures Through Rose Colored Glasses: Does Optimism Bias Toward Reproduction Discourage Anti-Natalism?, Faith L. Brown

Dissertations

In order to examine the possibility that individuals continue having children and holding positive views toward reproduction regardless of how much their future children might suffer because of an optimism bias, I conducted two experimental studies examining the effect of optimism manipulation on people’s opinions of anti-natalism, the position that it is morally wrong for individuals to reproduce. In Study 1, participants received an optimism (v. pessimism or control) manipulation about either themselves or a future child before being asked to read an essay about anti-natalism and a control essay having to do with parents being involved in school …

Evaluation Of Ocelot And Bobcat General And Reproductive Health In South Texas And Assessment Of Assisted Reproductive Techniques For Wild Felid Conservation, Ashley Reeves

Doctoral Dissertations

Recent studies of wild felid populations in southern Texas have shown loss of genetic variability and inbreeding depression over time, increasing the risk of reduced reproductive and immune fitness leading to an overall decrease in reproductive success, increase in disease susceptibility, and further population demise. Assessment of general health, pathogen exposure, and reproductive health can provide documentation of the possible effects of inbreeding and inform the development of conservation strategies. Assisted reproductive technologies have been used in many other species and can address behavioral or physical incompatibilities among breeding pairs, connect distant populations by transporting frozen gametes, preserve genetic diversity …

An Evaluation Of Bovine Reproductive Function From The Microrna Expression In The Female To Performance Testing In The Male, Erin Amiss

All Theses

Reproductive function is crucial to the success of a beef or dairy herd. Both the female and male play significant roles in the outcome of bovine reproduction. The bull contributes to reproductive success in many ways, such as genetics. The initial role that the bull has in reproductive success is fertilization, whether natural or by artificial insemination. One method of assessing bull performance are central bull test stations, which are traditionally administered by a university, extension center, or are privately owned. Central bull tests assess the performance of bulls from various management systems, breeds, and ages. Some measurements taken during …

Purification And Characterization Of An Anti-Basigin Monoclonal Antibody: Application To The Study Of Human Uterine Cells And Tissues, Jeannine M. Barna

All NMU Master's Theses

Between 1990 and 2017, the global rate of female infertility increased by over 15% (Sun et al., 2019). An embryo's failure to implant into the uterus is the primary cause of early pregnancy loss. Several studies have identified molecules necessary for successful implantation, but their function during implantation remains poorly understood (S. Zhang et al., 2013). The cell surface glycoprotein basigin-2 is necessary for embryo implantation in the mouse and plays a role in several cellular functions, including cell communication and extracellular matrix remodeling (Chen et al., 2009; K. Li & Nowak, 2020). In vitro studies using human uterine cells …

Role Of Nucleobindin-2/Nesfatin-1 In The Regulation Of Energetic Status And Maternal Mouthbrooding In The Cichlid Fish, Astatotilapia Burtoni, Saachi Chugh

LSU Master's Theses

Feeding and energetics are fundamental processes in all species that must also be balanced with reproductive investment. The peptide hormone nesfatin-1 is encoded by the nucleobindin-2(nucb2) gene and has recently emerged as a key player involved in both feeding and reproduction across vertebrates. How nesfatin-1 might help regulate trade-offs between feeding and reproduction, however, are relatively unexplored. The female cichlid fish Astatotilapia burtoniis an ideal system to examine the role of nesfatin-1 in regulating metabolic and reproductive functions because this species cycles between a gravid feeding state while preparing to mate, followed by a forced starvation …

Estimating Age In Mule Deer: Accuracy And Influence On Reproduction, Morgan S. Hinton

Theses and Dissertations

Accurate estimates of parameters and demographic rates of wildlife populations are crucial to management and conservation. One parameter of interest is age because it influences multiple demographic rates and behaviors including reproduction, dispersal, migration, and disease transmission. There are a number of methods used to estimate ages of wildlife. One of the most common methods used for mammals is evaluation of patterns in dental eruption and tooth wear. In the first chapter, we evaluate the accuracy associated with estimates of age from dental eruption and patterns of tooth wear for mule deer (Odocoileus hemionus). In the second chapter, we use …

The Impacts Of Climate Change On Blackeye Goby, Rhinogobiops Nicholsii, Stress Responses, Reproduction, And Offspring Fitness, Alora Yarbrough

Capstone Projects and Master's Theses

Along with warming and sea level rise, the increasing intensity of ocean acidification (OA) and hypoxia events in coastal environments is of large concern as climate change progresses. Weakened immune function, altered reproductive output, reduced aerobic scope, and hyperventilation are just some of the ways OA and hypoxia negatively affect fish. Under stress, such as OA or hypoxia, fish will produce the hormone cortisol to maintain homeostasis, so cortisol concentration can be used to determine the relative stress an animal is experiencing. This study evaluated the stress response of adult female blackeye gobies under both acute and chronic exposure to …

Reproductive Timing In Percina Aurora And Other Percina Species In The Pascagoula River Watershed, Malia Davidson

Honors Theses

The Southeastern United States is home to high levels of aquatic biodiversity and was recently named one of the Earth’s biodiversity hotspots. Within this region, growing human populations continue to place pressure on aquatic ecosystems and the resident species. Darters (Percidae: Percina) represent a large proportion of the freshwater fish diversity in the region. However, for some species the fundamentals of age, growth, reproductive timing and effort, and ecology remain poorly understood. That said, understanding the timing, duration, and effort allocated to reproduction is vital to successful management of any species. The pearl darter, Percina aurora, has been …

Induced Spawning Of Captive Tripletail Lobotes Surinamensis, Nicholas Adams

Master's Theses

The Tripletail, Lobotes surinamensis, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.

Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg^-1 for females, 550 IU.kg^-1 for males) pairs did not spawn. Pairs treated with GnRHa …

Notes From The Underground: An Exploration Of Burying Beetle Thermal Ecology, Sound Production And Reception, And Brain Morphology., Michelle Ward

Doctoral Dissertations

In a climatically and structurally changing world, it is important to understand how different thermal and social environments influence the behavior and physiology of the pillars of terrestrial ecosystems: insects. In this dissertation, I explored how insects, specifically Nicrophorus beetles, respond to biotic and abiotic challenges. Through this research, I addressed 1) how daily fluctuations in temperature influence Nicrophorus americanus reproductive success, 2) the context in which breeding pairs use stridulation during carcass burial, and subsequently how inhibition of stridulation influences reproductive success, 3) if the clypeal membrane on Nicrophorus beetles has the physiological properties of a tympanum, and if …

The Effects Of Roads On Movement, Flight Dynamics, Occupancy, And Productivity In Barn Owls (Tyto Alba), Brian Thomas Busby

Boise State University Theses and Dissertations

Barn Owls (Tyto alba) are a species of conservation concern in many portions of their cosmopolitan range. One important factor contributing to population declines and sometimes local extirpations is roads, which can cause direct mortality through Barn Owl-vehicle collisions, fragment habitat, limit dispersal and movement, and imperil long-term population viability. However, the effects of roads on Barn Owl reproduction are less clear. Further, the cumulative effects of roads on Barn Owls can be dependent on how they respond to them. Road and traffic responses of animals have been classified into four categories: 1) speeders, who increase speed to …

A Study Of The Effect Of Physiological Stressors On Hypothalamic Regulation Of Reproduction Using An In Vitro System, Lisa L. Amelse

Doctoral Dissertations

Stressors have a negative impact on reproductive efficiency in humans and other animals, which has an economic cost due to infertility treatments for humans and reduced income for food producers. We wished to determine the molecular pathways by which metabolic disturbances and low-level inflammation impact the hypothalamic pituitary gonadal (HPG) axis using an in vitro model. To this end, we used the GT1-7 cell line, an immortalized line expressing the Kiss1R receptor that responds to kisspeptin stimulation with the release of GnRH to assess the impact of stressors on the GnRH-releasing cells. Additionally, we used the KTaV-3 and KTaR-1[A1] …

Uno Student Access To Emergency Contraception: A Needs Assessment, Annika Kuchar

Theses/Capstones/Creative Projects

Emergency contraception (EC) is a common method of contraception that helps prevent ovulation before implantation of an egg inside a uterus. Among college students, EC can be utilized to prevent unplanned pregnancies that could potentially prevent someone from completing their degree. College students can face unique barriers when trying to access EC including transportation, privacy, financial, or mental/emotional barriers. The purpose of this study was to determine what barriers students at the University of Nebraska at Omaha (UNO) face when trying to access EC, and what future accommodations they would utilize if given the option. The study surveyed 147 UNO …