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Cloning And Sequencing Of Glucosyltransferase (Gt) Genes From Grapefruit (Citrus Paradisi) Seedlings, Searching For 7-O-Gt., Mebrahtu Berhane Sibhatu
Cloning And Sequencing Of Glucosyltransferase (Gt) Genes From Grapefruit (Citrus Paradisi) Seedlings, Searching For 7-O-Gt., Mebrahtu Berhane Sibhatu
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Flavonoids play crucial roles in plant life cycles and in human welfare. Grapefruit plants produce several glycosylated flavonoids and the glycosylation reactions are catalyzed by UDP-glucose: glucosyltransferases (GTs). Our objective was to use the SMART RACE RT-PCR strategy to obtain cDNA sequences of putative grapefruit flavonoid GTs. Gene specific primers were designed from the plant secondary product glucosyltransferase (PSPG) box and used to amplify 5’GT clones. Clone-specific primers were designed from 5’clones to amplify 3’ GT clones. We obtained 5 5’ clones and 1 3’ clone as candidate GTs. We used 3 of these clones to predict 2 compiled GT …