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Reproductive Life History And Signal Evolution In A Multi-Species Assemblage Of Electric Fish, Joseph Waddell
Reproductive Life History And Signal Evolution In A Multi-Species Assemblage Of Electric Fish, Joseph Waddell
Electronic Theses and Dissertations
Animals that co-occur in sympatry with multiple closely-related species use reproductive mate attraction signals not only to assess the quality of a potential conspecific mate (sexual selection), but also to discriminate conspecifics from heterospecifics (species recognition). However, the extent to which sexual selection and species recognition may interact, or even conflict, is poorly known. Neotropical electric fish offer unrivaled opportunities for understanding this problem. They generate simple, stereotyped mate attraction signals that are easy to record and quantify, and that are well-understood from the neurobiological perspective. Additionally, they live in electrically-crowded environments, where multiple congeners live and reproduce in close …
The Role Of The Y-Chromosome In The Evolution Of Autosomally Coded Traits, Ian Kutch
The Role Of The Y-Chromosome In The Evolution Of Autosomally Coded Traits, Ian Kutch
Electronic Theses and Dissertations
Recent work indicates that the Y-chromosome of the fruit fly Drosophila melanogaster can influence gene regulation on the autosomes and X chromosome. This newly discovered function of the Y has the potential to dramatically shape the regulatory evolution of numerous genes that reside throughout the genome; even for genes that code for both male and female traits. Given that the mechanism underlying the Y-linked influence on gene expression in D. melanogaster appears to exist in other independently evolved heterogametic sex chromosomes, the evolutionary implications of Y-linked regulatory variation (YRV) deserves to be explored. These implications include the potential for Y-chromosomes …
Using Molecular Genetic And Demographic Tools To Improve Management Of Ex Situ Avian Populations, Gina Ferrie
Using Molecular Genetic And Demographic Tools To Improve Management Of Ex Situ Avian Populations, Gina Ferrie
Electronic Theses and Dissertations
Small populations, specifically those that are isolated from others, are more prone to extinction than larger inter-connected populations. The risks that these small isolated populations face include loss of genetic diversity due to founder effects and inbreeding due to population bottlenecks, as well as demographic uncertainty due to fluctuating fecundity and mortality rates and impacts of external environmental factors. Ex situ populations, including those managed as conservation breeding programs with species recovery aims, as well as those that do not have reintroduction goals but are managed for long term population sustainability, suffer from the same extinction risks as small and …
The Effect Of Parental Population Density On Offspring Immune Function, Dana Davis
The Effect Of Parental Population Density On Offspring Immune Function, Dana Davis
Electronic Theses and Dissertations
It is well known that an individual's environment, genetic code, and gene by environment interactions have an effect on its overall phenotype. However, there is a growing body of work that shows that parents can have an effect on their offspring's phenotype beyond the inherited genetic code. Studies have shown that parents may affect their offspring through physiological mechanisms such as egg provisioning and epigenetic effects and through behavioral mechanisms such as maternal care. In many of these cases, the parental effect is triggered by an environmental cue. Previous work has shown that density can impact immune function and cuticle …
Habitat Distribution And Abundance Of Crayfishes In Two Florida Spring-Fed Rivers, Tiffani Manteuffel
Habitat Distribution And Abundance Of Crayfishes In Two Florida Spring-Fed Rivers, Tiffani Manteuffel
Electronic Theses and Dissertations
Crayfish are an economically and ecologically important invertebrate, however, research on crayfish in native habitats is patchy at best, including in Florida, even though the Southeastern U.S. is one of the most speciose areas globally. This study investigated patterns of abundance and habitat distribution of two crayfishes (Procambarus paeninsulanus and P. fallax) in two Florida spring-fed rivers (Wakulla River and Silver River, respectively). Study sites were surveyed once each season from April 2015 to March 2016 with baited minnow traps checked every other day, four times each survey. Habitat and environmental parameters evaluated included dominant vegetation or bottom type, percent …
Pyroglutamylated Amyloid Beta Peptides Enhance Non-Fibrillogenic Aggregation Of The Unmodified Peptide, Gregory Goldblatt
Pyroglutamylated Amyloid Beta Peptides Enhance Non-Fibrillogenic Aggregation Of The Unmodified Peptide, Gregory Goldblatt
Electronic Theses and Dissertations
Alzheimer's disease (AD) is accompanied by abnormal extracellular deposition of amyloid b (Ab) peptide. This has led to the amyloid cascade hypothesis, causatively relating Ab with AD. While Ab deposits assume a fibrillar cross-b structure, prefibrillar oligomers of Ab have been identified as the main cytotoxic agents in AD. Pyroglutamylated amyloid beta (AbpE) peptides are N-terminally truncated and pyroglutamylated (at Glu3 or Glu11) Ab molecules that display enhanced cytotoxicity and represent up to 50% of total Ab in AD brains. AbpE significantly enhances the toxicity of unmodified Ab by an unknown mechanism. Although in situ Ab populations are heterogeneous, the …
Assessment Of Staphylococcus Aureus Genetics: Clinical Versus Community Epidemiology, Matthew Lawrance
Assessment Of Staphylococcus Aureus Genetics: Clinical Versus Community Epidemiology, Matthew Lawrance
Electronic Theses and Dissertations
Staphylococcus aureus has an historical relationship with anthropogenic environments, particularly hospitals, where infection characteristics differ from community-acquired disease. This has promoted a designation of strains as healthcare or community associated. Despite this affiliation, genetic approaches have failed to support these groupings. In order to establish the genetic relationship between S. aureus from differing anthropogenic environments, I have analyzed the relatedness between three cohorts of S. aureus: nasal carriage isolates from community participants, infectious isolates from hospitals, and a cohort from an uninvestigated environment, an ambulatory clinic. Multilocus Sequence Typing (MLST) and Staphylococcus aureus protein a (spa) repeat regions were analyzed …
Transforming The Aquatic Urban Landscape: Nutrient Status And Management Of Stormwater Basins, Lindsay Skovira
Transforming The Aquatic Urban Landscape: Nutrient Status And Management Of Stormwater Basins, Lindsay Skovira
Electronic Theses and Dissertations
Urbanization is a largely irreversible anthropogenic change that degrades environmental quality, including aquatic ecosystems. Stormwater ponds are a popular best management practice (BMP) to mitigate the effects of urban land use on downstream water bodies and contribute significantly to the total area of aquatic ecosystems in some urban watersheds. My research investigated the distribution of stormwater ponds and examined how different urban land uses influenced biophysicochemical conditions and management of those ponds in a rapidly developing suburban watershed in the Econlockhatchee River basin in Florida, USA. I evaluated limnological and ecological parameters in randomly-selected ponds distributed among three urban land-use …
Role Of Kruppel-Like Factor 8 (Klf8) In Cancer And Cardiomyopathy, Satadru Lahiri
Role Of Kruppel-Like Factor 8 (Klf8) In Cancer And Cardiomyopathy, Satadru Lahiri
Electronic Theses and Dissertations
Cancer and cardiovascular diseases are two most fatal diseases causing innumerable death each year. Understanding the mechanisms underlying these diseases is critical for developing proper therapeutic approach. Kruppel-like factor 8 (KLF8) is a member of Kruppel-like family transcription factors that is overexpressed in many types of cancers. There is no report on role of KLF8 in cardiovascular diseases to date. KLF8 transcriptionally activates or represses a host of target genes to promote cancer cell proliferation, migration, invasion and epithelial to mesenchymal transition during tumor progression. Studies proposed in this thesis identified a novel posttranslational modification of KLF8 essential for its …
Involvement Of Mirnas In The Development Of Androgen Independent Prostate Cancer, Richard Ottman
Involvement Of Mirnas In The Development Of Androgen Independent Prostate Cancer, Richard Ottman
Electronic Theses and Dissertations
Development of resistance to androgen deprivation therapy (ADT) is a major obstacle for the management of advanced prostate cancer. Therapies with androgen receptor (AR) antagonists and androgen withdrawal initially result in tumor regression but development of compensatory mechanisms including AR bypass signaling leads to tumor re-growth, independent of circulating androgens. The result is the emergence of castration resistant prostate cancer (CRPC), a highly morbid disease exhibiting aberrant expression of many protein-coding and non-coding genes. Under the umbrella of non-coding RNAs is a class of small regulatory RNAs referred to as microRNAs (miRNAs). MicroRNAs are believed to function in the maintenance …
Flying Under The Lidar: Relating Forest Structure To Bat Community Diversity, Anna Schneider Swanson
Flying Under The Lidar: Relating Forest Structure To Bat Community Diversity, Anna Schneider Swanson
Electronic Theses and Dissertations
Bats are important to many ecological processes such as pollination, insect (and by proxy, disease) control, and seed dispersal and can be used to monitor ecosystem health. However, they are facing unprecedented extinction risks from habitat degradation as well as pressures from pathogens (e.g., white-nose syndrome) and wind turbines. LiDAR allows ecologists to measure structural variables of forested landscapes with increased precision and accuracy at broader spatial scales than previously possible. This study used airborne LiDAR to classify forest habitat/canopy structure at the Ordway-Swisher Biological Station (OSBS) in north central Florida. LiDAR data were acquired by the National Ecological Observatory …
Pasture Management, The Red Imported Fire Ant (Solenopsis Invicta) And Dung Beetle Mediated Ecosystem Services, Christen Steele
Pasture Management, The Red Imported Fire Ant (Solenopsis Invicta) And Dung Beetle Mediated Ecosystem Services, Christen Steele
Electronic Theses and Dissertations
Grazing animals in Florida deposit over 20 million metric tons of dung per year, making dung a significant non-point source of pollution (extrapolated from Fincher, 1981). Degradation of this dung occurs naturally, primarily due to a diverse group of beetles (Order Coleoptera) primarily in the families Scarabaeidae and Geotrupidae(hereafter dung beetles). Dung that is not degraded becomes a non-point source of pollutants and may be leached into water bodies. Additionally, dung provides an incubation site for the pests and parasites of both humans and livestock. Thus, as dung beetles consume and degrade dung, they provide a multitude of ecosystem services …
From Pet To Pest? The Potential Global Range And Food Web Effects Of A Generalist Carnivore, Hannah Bevan
From Pet To Pest? The Potential Global Range And Food Web Effects Of A Generalist Carnivore, Hannah Bevan
Electronic Theses and Dissertations
The Nile monitor lizard [Varanus niloticus (Linnaeus, 1766)] is a generalist carnivore, native to Sub-Saharan Africa and the Nile River but now established in North America as a result of the pet trade. Once introduced, they are a potential invasive threat to native wildlife. Here, I create ensemble species distribution models (SDMs) to predict the global distribution of this generalist carnivore given current and future climate conditions. I then quantify the monitor's potential effects on 85 food webs representing >900 different species within the projected regions based on stomach content data. Climate, vegetation, and elevation data are used for 507 …
Effects Of Harmful Algal Blooms Caused By Aureoumbra Lagunensis (Brown Tide) On Larval And Juvenile Life Stages Of The Eastern Oyster (Crassostrea Virginica), Panagiota Makris
Effects Of Harmful Algal Blooms Caused By Aureoumbra Lagunensis (Brown Tide) On Larval And Juvenile Life Stages Of The Eastern Oyster (Crassostrea Virginica), Panagiota Makris
Electronic Theses and Dissertations
Harmful algal blooms caused by the marine microalga Aureoumbra lagunensis have been associated with negative impacts on marine fauna, both vertebrate and invertebrate. Within the Indian River Lagoon (IRL) estuary system along Florida's east coast, blooms of A. lagunensis in excess of 1×106 cells mL-1 have occurred along with higher than average salinities (> 35 PSU) during times of peak reproduction and growth for the eastern oyster Crassostrea virginica. Field and laboratory studies were used to investigate the effects of A. lagunensis and high salinities on early life stages of eastern oysters, late pediveliger to early juvenile. Natural recruitment of …
A High-Content Multiplexed Screening Platform For The Evaluation And Manipulation Of Force And Fatigue Of Adult Derived Skeletal Muscle Myotubes In Defined Serum-Free Medium, Christopher Mcaleer
A High-Content Multiplexed Screening Platform For The Evaluation And Manipulation Of Force And Fatigue Of Adult Derived Skeletal Muscle Myotubes In Defined Serum-Free Medium, Christopher Mcaleer
Electronic Theses and Dissertations
The overall focus of this project has two parts: First, was to develop a protocol utilizing serum-free media formulations and defined plating and culture techniques to create functional in vitro myotubes derived from adult skeletal muscle satellite cells. The second was to manipulate the inherent muscle parameters such as force output and fatigue of these myotubes by employing exercise regimes or by small molecule application. The importance of serum-free medium use for in vitro cultures is becoming increasingly important in creating functional systems that can be validated for drug testing by the Food and Drug Administration (FDA). Also, the study …
Identifying Inundation-Driven Effects Among Intertidal Crassostrea Virginica In A Commercially Important Gulf Of Mexico Estuary, Joshua Solomon
Identifying Inundation-Driven Effects Among Intertidal Crassostrea Virginica In A Commercially Important Gulf Of Mexico Estuary, Joshua Solomon
Electronic Theses and Dissertations
Sea level rise and changing storm frequency and intensity resulting from climate change create tremendous amounts of uncertainty for coastal species. Intertidal species may be especially affected since they are dependent on daily inundation and exposure. The eastern oyster Crassostrea virginica is an economically and biologically important sessile intertidal species ranging from Canada to the Gulf of Mexico. Declines and changes in distribution of oyster populations has forced commercial harvesting to spread from subtidal to intertidal reefs. We investigated the potential responses of intertidal C. virginica to sea level rise, and the response of larval settlement to sedimentation which is …
Testing For Isolation In Florida Raccoons (Procyon Lotor) Using Phylogenetics And Population Genetic Structure, Alexa Trujillo
Testing For Isolation In Florida Raccoons (Procyon Lotor) Using Phylogenetics And Population Genetic Structure, Alexa Trujillo
Electronic Theses and Dissertations
Molecular data are useful in determining if populations are isolated and for species delimitation. Researchers and managers currently recognize five subspecies of raccoons (Procyon lotor) in Florida, based largely on perceived geographic isolation due to the island ranges of four subspecies. In this study, I provide the first estimate of phylogenetic relationships and population divergences within Florida raccoons using a molecular dataset. I analyze the mitochondrial control region, cytochrome b gene, and eight nuclear microsatellite loci to test two hypotheses: 1) the five, morphologically and geographically-defined subspecies of raccoon in Florida represent genetically distinct populations and (2) due to differing …
Development Of Human And Rodent Based In Vitro Systems Toward Better Translation Of Bench To Bedside In Vivo Results, Bonnie Berry
Development Of Human And Rodent Based In Vitro Systems Toward Better Translation Of Bench To Bedside In Vivo Results, Bonnie Berry
Electronic Theses and Dissertations
Prospective medicinal compounds progress through multiple testing phases before becoming licensed drugs. Testing of novel compounds includes a preclinical phase where the potential therapeutic is tested in vitro and/or in animal models in vivo to predict its potential efficacy and/or toxicity in humans. The failure of preclinical models to accurately predict human drug responses can lead to potentially dangerous compounds being administered to humans, or potentially beneficial compounds being kept in development abeyance. Moreover, inappropriate choice in model organism for studying disease states may result in pushing forward inappropriate drug targets and/or compounds and wasting valuable time and resources in …
Genetic Structure And Demographic Analysis Of Key Deer (Odocoileus Virginianus Clavium), Vicki Villanova
Genetic Structure And Demographic Analysis Of Key Deer (Odocoileus Virginianus Clavium), Vicki Villanova
Electronic Theses and Dissertations
Recent improvements in genetic analyses have paved the way in using molecular data to answer questions regarding evolutionary history, genetic structure, and demography. Key deer are a federally endangered subspecies assumed to be genetically unique (based on one allozyme study), homogeneous, and have a female-biased population of approximately 900 deer. I used 985bp of the mitochondrial cytochrome b gene and 12 microsatellite loci to test two hypotheses: 1) if the Moser Channel is a barrier to gene flow, I should expect that Key deer are differentiated and have reduced diversity compared to mainland deer and (2) if isolation on islands …
Does The Journey Matter More Than The Destination? The Contribution Of Geospatial And Local Conditions To Invasive Pomacea Maculata Distribution Across Ranchland Wetlands, Steffan Pierre
Electronic Theses and Dissertations
Geospatial characteristics such as isolation and avenues of connectivity influence an invader's pattern of dispersal and distribution. However many examinations of invasion success ignore the contribution of dispersal to patterns of invasion and focus only on the local environmental/habitat factors. This study examines the interaction of geospatial characteristics, that may influence dispersal, and local environmental factors, that may govern successful occupation, on the likelihood of invasion (invasability) of wetlands within an agriculturally modified landscape. I examined the current invasion of seasonal wetlands in south-central Florida ranchland by non-native apple snails (Pomacea maculata (Ampullariidae)) as a model system for understanding this …
A Binary Approach For Selective Recognition Of Nucleic Acids And Proteins, Evan Cornett
A Binary Approach For Selective Recognition Of Nucleic Acids And Proteins, Evan Cornett
Electronic Theses and Dissertations
The design of probes for the selective recognition of biopolymers (nucleic acids and proteins) is a fundamental task for studying, diagnosing, and treating diseases. Traditional methods utilize a single component (small molecule or oligonucleotide) that binds directly to the target biopolymer. However, many biopolymers are unable to be targeted with this approach. The overarching goal of this dissertation is to explore a new, binary approach for designing probes. The binary approach requires two components that cooperatively bind to the target, triggering a recognition event. The requisite binding of two-components allows the probes to have excellent selectivity and modularity. The binary …
Quantifying The Effects Of Boat Wakes On Intertidal Oyster Reefs In A Shallow Estuary, Donna Campbell
Quantifying The Effects Of Boat Wakes On Intertidal Oyster Reefs In A Shallow Estuary, Donna Campbell
Electronic Theses and Dissertations
There have long been concerns about the negative impacts of recreational boating activity in the Indian River Lagoon system (IRL), especially in Mosquito Lagoon (ML), the northernmost part of the IRL. My research is focused on the impacts of boat wakes on intertidal reefs formed by the eastern oyster, Crassostrea virginica. There has been a 24% loss of oyster habitat in ML since 1943, where natural oyster reefs have been replaced by dead oyster reefs which do not serve the same ecological function. While there is anecdotal and correlative evidence that this loss is a result of boat wakes, no …
A Socio-Economic Assessment Of Marine Turtle Eco-Tourism, Kendra Cope
A Socio-Economic Assessment Of Marine Turtle Eco-Tourism, Kendra Cope
Electronic Theses and Dissertations
Marine turtles have historically contributed to economic activity through consumptive harvest for food, tools, and decorative objects. Only recently have they begun to benefit humans economically through non-consumptive roles, primarily as a focal point of educational eco-tourism. In recent years, the annual number of turtle walks conducted around the Archie Carr National Wildlife Refuge (ACNWR) has risen. This expansion contributed to a statistically significant increase in attendance from 2,162 in 2001 to 3,047 participants in 2014. I examined the regional economic impacts of marine turtle eco-tourism around the ACNWR using social surveys and an economic impact analysis tool. IMPLAN, an …
Modeling And Analysis Of A Three-Species Food Web With Facilitated And Intraguild Predation, Joshua Castro
Modeling And Analysis Of A Three-Species Food Web With Facilitated And Intraguild Predation, Joshua Castro
Electronic Theses and Dissertations
Biotic interactions are known to shape natural community assemblages and biodiversity. Positive interactions such as facilitation have recently received attention in ecological food webs. Mechanistic models have improved our understanding of these complex food web interactions. Here, focus is given to a three-species food web system with a beach dune natural community in mind. In the last decade, there has been a series of studies investigating intraguild predation between two major loggerhead sea turtle nest predators, North American raccoons and Atlantic ghost crabs. Studies have also highlighted that ghost crab predation assists raccoons in finding nests (i.e., facilitated predation). However, …
The Effect Of Landscape Variables On Adult Mosquito (Diptera:Culicidae)Diversity And Behavior, Caitlyn Debevec
The Effect Of Landscape Variables On Adult Mosquito (Diptera:Culicidae)Diversity And Behavior, Caitlyn Debevec
Electronic Theses and Dissertations
Diseases vectored by mosquitoes cause millions of deaths each year. In modern times Florida*s disease risk has been reduced due to efforts to lessen the prevalence of mosquitoes through habitat modification of non-adults. With emerging diseases (i.e. Dengue and Chikunguya) encroaching into Florida from the Caribbean, this traditional approach may not be enough. Alternatively, we can better understand the ecology of how disease works in an ecosystem. One possible way is through the Dilution Effect, which states that the more species that are in a system the lower the chance for zoonosis. This project models mosquito diversity across regions, land …
A Time-Course Analysis Of Behavioral Plasticity And Differential Gene Expression Patterns In Response To Density In Schistocerca Americana (Orthoptera: Acrididae), Steven Gotham
Electronic Theses and Dissertations
Phenotypic plasticity is the ability of the genotype to express alternative phenotypes in response to different environmental conditions and this is considered to be an adaptation in which a species can survive and persist in a rapidly changing environment. Some grasshoppers and locusts are capable of expressing an extreme form of density-dependent phenotypic plasticity, known as locust phase polyphenism. At low population density, the individuals typically have a cryptic coloration as nymphs, are less active, and only seek out conspecifics for reproductive purposes. At high density, however, they develop a drastically different phenotype in which they have a conspicuous coloration, …
Effects Of Weaning Age On Body Composition And Growth Of Ex Situ California Sea Lion (Zalophus Californianus) Pups, Brandon Davis
Effects Of Weaning Age On Body Composition And Growth Of Ex Situ California Sea Lion (Zalophus Californianus) Pups, Brandon Davis
Electronic Theses and Dissertations
Pinnipeds exhibit a wide range of lactation strategies that vary from just a few days to nearly three years in duration. Phocids have a relatively short, intense nursing period culminating with weaning after just a few days or weeks, while dependent otariids generally take several months of consuming a combined milk and solid food diet before being completely independent. The transition to nutritional independence can be particularly challenging for newly weaned pups, which must adjust to behavioral, physiological and nutritional changes as a milk diet is replaced with solid food. An interruption in energy resources during this formative stage could …
Integral Projection Models Reveal Interactive Effects Of Biotic Factors And Disturbance On Plant Demography, Matthew Tye
Integral Projection Models Reveal Interactive Effects Of Biotic Factors And Disturbance On Plant Demography, Matthew Tye
Electronic Theses and Dissertations
Understanding factors limiting population growth is crucial to evaluating species persistence in changing environments. I used Integral Projection Models (IPMs) to elucidate the role of biotic interactions and disturbance on population growth rate in two plants: Helianthemum squamatum, a perennial endemic to gypsum habitats in central Spain, and Liatris ohlingerae, a long-lived perennial endemic to the Lake Wales Ridge of central Florida. In H. squamatum, there was a strong positive effect of trampling in the site with the highest plant density and moderate positive effects of seed addition in the site with the lowest plant density. Differences in treatment effectiveness …
Effects Of Biotic Interactions On Coastal Wetland Communities With Applications For Restoration, Melinda Donnelly
Effects Of Biotic Interactions On Coastal Wetland Communities With Applications For Restoration, Melinda Donnelly
Electronic Theses and Dissertations
Coastal wetland plants serve as ecological engineers in a physiologically stressful environment and the loss of coastal wetlands can cause negative effects throughout the estuarine system. Due to increased degradation of coastal habitats worldwide, interest in restoration has increased around the world. An understanding of the biotic processes affecting species distribution and diversity is critical for future conservation, management, and restoration of coastal wetlands. The purpose of my study was to test the effects of biotic interactions on native coastal wetland plants and determine how these interactions may be incorporated into current and future restoration projects. I had three primary …
Using Land Cover Mapping And Landscape Metrics To Evaluate Effects Of Urban Development On Ecological Integrity In Florida, Michael Gilbrook
Using Land Cover Mapping And Landscape Metrics To Evaluate Effects Of Urban Development On Ecological Integrity In Florida, Michael Gilbrook
Electronic Theses and Dissertations
The widespread loss and degradation of habitat constitutes the largest threat to biodiversity in North America. While regulatory programs such as the Endangered Species Act of 1973 and wetland permitting under the Clean Water Act have addressed acute assaults on critical habitat, large areas of unprotected uplands have been lost. Urban development, particularly the advent of lower density suburban and rural sprawl, has greatly diminished the extent of contiguous patches of forest habitat and introduced a host of other undesirable effects on ecosystem function. This study sought to evaluate the extent of urban sprawl and its effects on ecological integrity …