Digital Commons Network^™

A Label-Free Assay For Aminoacylation Of Trna, Howard Gamper, Ya-Ming Hou

Department of Biochemistry and Molecular Biology Faculty Papers

Aminoacylation of tRNA generates an aminoacyl-tRNA (aa-tRNA) that is active for protein synthesis on the ribosome. Quantification of aminoacylation of tRNA is critical to understand the mechanism of specificity and the flux of the aa-tRNA into the protein synthesis machinery, which determines the rate of cell growth. Traditional assays for the quantification of tRNA aminoacylation involve radioactivity, either with a radioactive amino acid or with a [3'-32P]-labeled tRNA. We describe here a label-free assay that monitors aminoacylation by biotinylation-streptavidin (SA) conjugation to the α-amine or the α-imine of the aminoacyl group on the aa-tRNA. The conjugated aa-tRNA product is readily …

We Are...Marshall, October 7, 2020, Office Of Marshall University Communications

We Are ... Marshall: the Newsletter for Marshall University 1999-2023

No abstract provided.

Cyber Supply Chain Risk Management: Toward An Understanding Of The Antecedents To Demand For Assurance, Clark Hampton, Steve G. Sutton, Vicky Arnold, Deepak Khazanchi

Information Systems and Quantitative Analysis Faculty Publications

Recognizing the need for effective cyber risk management processes across the supply chain, the AICPA issued a new SOC in March 2020 for assuring cyber supply chain risk management (C-SCRM) processes. This study examines supply chain relationship factors and cyber risk issues to better understand the demand for C-SCRM assurance. Resource-Advantage Theory of Competition provides the conceptual foundation for assessing the dual drivers of relationship building and cyber risk management on demand for assurance. We use a field survey to collect data from 205 professionals enabling evaluation of the complex relationships in the theoretical model. Results support all hypotheses, provide …

Change Management: Increasing The Odds Of Adoption, Travis Mcneal

Operations Management Presentations

With rapid changes happening all around, organizations are finding themselves in positions where they need their workforce to adapt to new ways of working (technology, structural, process). Organizations develop strategies, programs and projects intended to address these changes. Unfortunately, history is littered with strategies and programs that fail to live deliver on expected results. This has led to the recent rise and proliferation of Change Management as a practice and skill set. Often times, however, Change Management theory is given preeminence over practicality. This webinar will explore practical and actionable ways you can take to immediately increase the odds …

2019 Pulaski County Education Report Card, Sarah Mckenzie

Education Report Card

Our goal is to help parents, school personnel, community members, and policy makers understand how effectively the students in their community are being served.

Pulaski students are demonstrating similar growth in achievement on the ACT Aspire as students in the state overall, but in achievement, graduation rates, and School Quality and Student Success, Pulaski County schools are performing below the state average.

This report card presents information about these key performance indicators for the districts in Pulaski counties. The Growth, Achievement, School Quality, and Overall scores of the four traditional districts are compared to the average for that group, and …

Overcoming Barriers For Sirna Therapeutics: From Bench To Bedside, Muhammad Imran Sajid, Muhammad Moazzam, Shun Kato, Kayley Yeseom Cho, Rakesh Kumar Tiwari

Pharmacy Faculty Articles and Research

The RNA interference (RNAi) pathway possesses immense potential in silencing any gene in human cells. Small interfering RNA (siRNA) can efficiently trigger RNAi silencing of specific genes. FDA Approval of siRNA therapeutics in recent years garnered a new hope in siRNA therapeutics. However, their therapeutic use is limited by several challenges. siRNAs, being negatively charged, are membrane-impermeable and highly unstable in the systemic circulation. In this review, we have comprehensively discussed the extracellular barriers, including enzymatic degradation of siRNAs by serum endonucleases and RNAases, rapid renal clearance, membrane impermeability, and activation of the immune system. Besides, we have thoroughly described …

Job Description : Board Of Trustees Representative, University Of Maine Student Government, Inc.

General University of Maine Publications

Job description for a one-year appointment to the position of Representative to the Board of Trustees for University of Maine Student Government. Includes list of required tasks and base pay rate.

October 7, 2020: The One Religious Question Barrett Should Not Have To Answer, Bruce Ledewitz

Hallowed Secularism

Blog post, “The One Religious Question Barrett Should Not Have to Answer“ discusses politics, theology and the law in relation to religion and public life in the democratic United States of America.

School Of Nursing News, Georgia Southern University

School of Nursing News (2013-2023)

• RN-BSN Program Receives National Rankings

Restoration Of Seagrass Habitat Leads To Rapid Recovery Of Coastal Ecosystem Services, Robert J. Orth, Jonathan S. Lefcheck, Karen S. Mcglathery, Lillian Aoki, Mark Luckenbach, Kenneth A. Moore, Matthew P.J. Oreska, Richard A. Snyder, David J. Wilcox, Bo Lusk

VIMS Articles

There have been increasing attempts to reverse habitat degradation through active restoration, but few largescale successes are reported to guide these efforts. Here, we report outcomes from a unique and very successful seagrass restoration project: Since 1999, over 70 million seeds of a marine angiosperm, eelgrass (Zostera marina), have been broadcast into mid-western Atlantic coastal lagoons, leading to recovery of 3612 ha of seagrass. Well-developed meadows now foster productive and diverse animal communities, sequester substantial stocks of carbon and nitrogen, and have prompted a parallel restoration for bay scallops (Argopecten irradians). Restored ecosystem services are approaching historic levels, but we …

Periodic Report: Update On Outstanding Lending Facilities Authorized By The Board Under Section 13(3) Of The Federal Reserve Act - October 7, 2020, Federal Reserve System: Board Of Governors

Documents

No abstract provided.

Passing: Dwight Webb, Susan Dumais

UNH Today Archive

No abstract provided.

Flu Clinics At Health & Wellness, Unh Communications And Public Affairs

UNH Today Archive

No abstract provided.

Defrosting The Arctic, Robbin Ray

UNH Today Archive

No abstract provided.

Outdoor Groupies, Jody Record

UNH Today Archive

No abstract provided.

Unh Scientists Receive $2m Grant To Support Organic Dairy Industry, Lori Tyler Gula

UNH Today Archive

No abstract provided.

Electing A President: History Repeats Itself, Jody Record

UNH Today Archive

No abstract provided.

Sb-20f-3515: Changes To Activity & Service Fee Budget Provisionary Language Fiscal Year 2020/2021, Student Government Association University Of North Florida

Legislation

No abstract provided.

Resistive Switching In Fto/Cuo-Cu2o/Au Memory Devices, Amir Shariffar, Haider Salman, Tanveer A. Siddique, Wafaa Gebril, M. Omar Manasreh

Electrical Engineering Faculty Publications and Presentations

Memristors are considered to be next-generation non-volatile memory devices owing to their fast switching and low power consumption. Metal oxide memristors have been extensively investigated and reported to be promising devices, although they still suffer from poor stability and laborious fabrication process. Herein, we report a stable and power-efficient memristor with novel heterogenous electrodes structure and facile fabrication based on CuO-Cu2O complex thin films. The proposed structure of the memristor contains an active complex layer of cupric oxide (CuO) and cuprous oxide (Cu2O) sandwiched between fluorine-doped tin oxide (FTO) and gold (Au) electrodes. The fabricated memristors demonstrate bipolar resistive switching …

Improving The State Of Society And Making Men Happy: Rhetorical Citizenship In Historical And Contemporary Vegetarian/Vegan Discourses, Kristin E. Kondrlik

Sustainability Research & Practice Seminar Presentations

No abstract provided.

The Srg Rat, A Sprague-Dawley Rag2/Il2rg Double-Knockout Validated For Human Tumor Oncology Studies, Fallon K. Noto, Jaya Sangodkar, Bisoye Towobola Adedeji, Sam Moody, Christopher B. Mcclain, Ming Tong, Eric Ostertag, Jack Crawford, Xiaohua Gao, Lauren Hurst, Caitlin M. O'Connor, Erika N. Hanson, Sudeh Izadmehr, Rita Tohmé, Jyothsna Narla, Kristin Lesueur, Kajari Bhattacharya, Amit Rupani, Marwan K. Tayeh, Jeffrey W. Innis, Matthew D. Galsky, B. Mark Evers, Analisa Difeo, Goutham Narla, Tseten Y. Jamling

Markey Cancer Center Faculty Publications

We have created the immunodeficient SRG rat, a Sprague-Dawley Rag2/Il2rg double knockout that lacks mature B cells, T cells, and circulating NK cells. This model has been tested and validated for use in oncology (SRG OncoRat®). The SRG rat demonstrates efficient tumor take rates and growth kinetics with different human cancer cell lines and PDXs. Although multiple immunodeficient rodent strains are available, some important human cancer cell lines exhibit poor tumor growth and high variability in those models. The VCaP prostate cancer model is one such cell line that engrafts unreliably and grows irregularly in …

The Freshman Experience: Establishing Normalcy In Quarantine, Claire Needs

The Spectator Online

No abstract provided.

The Parthenon, October 7, 2020, Douglas Harding, Denise Jackson, Meg Keller, Brittany Hively, Isabella Robinson, Taylor Huddleston, Grant Goodrich, Zach Hiser

The Parthenon

No abstract provided.

Distillers Grains Pre, During, And Post Covid-19: Ongoing Recovery And Structural Demand Implications, Daniel Gertner, Elliott James Dennis

Cornhusker Economics

Prior to the shutdowns caused by the COVID-19 pandemic, distillers grains and other co-products of ethanol production played a crucial role in maintaining the rev-enue stream of dry mill ethanol plants (Irwin 2020). With low crude oil prices over the last half of the decade and corn prices holding steady over the same period of time, the margin for error in the ethanol industry was thin. More than a third of Nebraska’s ethanol plants either temporarily or permanently closed in 2019 (MacroTrends 2020); (York News Times 2019). To prevent similar outcomes, ethanol plants focused on diver-sifying their operations to produce …

Seahawks Stop The Patriots In A Sunday Night Showdown: Another Win 35-30, Tara King Goldman

The Spectator Online

No abstract provided.

Seattle Community Raises $20,000 To Support Laid Off Dining Hall Workers, Logan Gilbert

The Spectator Online

No abstract provided.

Students Adjust To Life On Campus During Covid-19, Michaela Moore

The Spectator Online

No abstract provided.

Differential Survival Of Nursery‐Reared Acropora Cervicornis Outplants Along The Florida Reef Tract, Robert Van Woesik, Raymond B. Banister, Erich Bartels, David S. Gilliam, Elizabeth A. Goergen, Caitlin Lustic, Kerry Maxwell, Amelia Moura, Erinn M. Muller, Stephanie Schopmeyer, R. Scott Winters, Diego Lirman

Marine & Environmental Sciences Faculty Articles

In recent decades, the Florida reef tract has lost over 95% of its coral cover. Although isolated coral assemblages persist, coral restoration programs are attempting to recover local coral populations. Listed as threatened under the Endangered Species Act, Acropora cervicornis is the most widely targeted coral species for restoration in Florida. Yet strategies are still maturing to enhance the survival of nursey‐reared outplants of A. cervicornis colonies on natural reefs. This study examined the survival of 22,634 A. cervicornis colonies raised in nurseries along the Florida reef tract and outplanted to six reef habitats in seven geographical subregions between 2012 …

Cochrane Corner Summary Of Review Titled: “Peer Support For People With Schizophrenia Or Other Serious Mental Illness”, Beverley Ewens, Amanda Barnard-Towell, Sheila Mortimer-Jones, Vivien Kemp, Amanda Cole

Research outputs 2014 to 2021

Serious mental illnesses affect how people feel, think, and behave, and frequently contribute to disability in psychosocial and occupational functioning and quality of life (American Psychiatric Association 2015; Harvey & Strassnig, 2012; Mohamed et al., 2008). Psychosocial interventions designed to support people with schizophrenia and their families have shown to improve the person’s rehabilitation, reintegration into the community, and recovery (The National Collaborating Centre for Mental Health, 2009; Pharoah et al., 2012). Peer workers are defined as individuals with lived experience of mental illness (Aguey-Zinsou et al., 2018), with peer support principles based on shared responsibility and mutual respect (Mead …

A Prognostic Gene Expression Signature For Oropharyngeal Squamous Cell Carcinoma, Xinyi Liu, Ping Liu, Rebecca D Chernock, Krystle A Lang Kuhs, James S Lewis, Jingqin Luo, Hiram A Gay, Wade L Thorstad, Xiaowei Wang

Open Access Publications

BACKGROUND: Robust prognostic stratification of patients with oropharyngeal squamous cell carcinoma (OPSCC) is important for developing individualized treatment plans. This study was conducted to develop and validate a clinically feasible prognostic classifier based on transcriptome-wide gene expression profiles.

METHODS: Tumor tissues were collected from 208 OPSCC patients treated at Washington University in St. Louis and 130 OPSCC patients treated at Vanderbilt University, used for model training and validation, respectively. OPSCC patients (n = 70) from the TCGA cohort were also included for independent validation. Based on RNA-seq profiling data, Cox proportional hazards regression analysis was performed to identify genes associated …