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2018

Legume

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Capturing Variation In Lens (Fabaceae): Development And Utility Of An Exome Capture Array For Lentil, Ezgi Ogutcen, Larissa Ramsay, Eric Bishop Von Wettberg, Kirstin E. Bett Jun 2018

Capturing Variation In Lens (Fabaceae): Development And Utility Of An Exome Capture Array For Lentil, Ezgi Ogutcen, Larissa Ramsay, Eric Bishop Von Wettberg, Kirstin E. Bett

College of Agriculture and Life Sciences Faculty Publications

Premise of the Study: Lentil is an important legume crop with reduced genetic diversity caused by domestication bottlenecks. Due to its large and complex genome, tools for reduced representation sequencing are needed. We developed an exome capture array for use in various genetic diversity studies. Methods: Based on the CDC Redberry draft genome, we developed an exome capture array using multiple sources of transcript resources. The probes were designed to target not only the cultivated lentil, but also wild species. We assessed the utility of the developed method by applying the generated data set to population structure and phylogenetic analyses. …

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Alternative Legume Species Can Reduce The Environmental Impacts Of Cattle, Elizabeth K. Stewart, Jennifer W. Macadam, Juan J. Villalba Jun 2018

Alternative Legume Species Can Reduce The Environmental Impacts Of Cattle, Elizabeth K. Stewart, Jennifer W. Macadam, Juan J. Villalba

All Current Publications

This fact sheet describes how alternative legume species can reduce the environmental impacts of cattle, grass versus legumes as livestock feed, benefits, and producer concerns.

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Genetic And Molecular Mechanisms Underlying Symbiotic Specificity In Legume-Rhizobium Interactions, Qi Wang, Jinge Liu, Hongyan Zhu Mar 2018

Genetic And Molecular Mechanisms Underlying Symbiotic Specificity In Legume-Rhizobium Interactions, Qi Wang, Jinge Liu, Hongyan Zhu

Plant and Soil Sciences Faculty Publications

Legumes are able to form a symbiotic relationship with nitrogen-fixing soil bacteria called rhizobia. The result of this symbiosis is to form nodules on the plant root, within which the bacteria can convert atmospheric nitrogen into ammonia that can be used by the plant. Establishment of a successful symbiosis requires the two symbiotic partners to be compatible with each other throughout the process of symbiotic development. However, incompatibility frequently occurs, such that a bacterial strain is unable to nodulate a particular host plant or forms nodules that are incapable of fixing nitrogen. Genetic and molecular mechanisms that regulate symbiotic specificity …

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Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault Jan 2018

Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Transcriptomic approaches revealed thousands of genes differentially or specifically expressed during nodulation, a biological process resulting from the symbiosis between leguminous plant roots and rhizobia, atmospheric nitrogen-fixing symbiotic bacteria. Ultimately, nodulation will lead to the development of a new root organ, the nodule. Through functional genomic studies, plant transcriptomes have been used by scientists to reveal plant genes potentially controlling nodulation. However, it is important to acknowledge that the physiology, transcriptomic programs, and biochemical properties of the plant cells involved in nodulation are continuously regulated. They also differ between the different cell-types composing the nodules. To generate a more accurate …

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