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Evaluation Of Fourier Transform Infrared Spectroscopy Measurements Of Glucose And Xylose In Biomass Hydrolyzate, Czarena L. Crofcheck, Michael D. Montross
Evaluation Of Fourier Transform Infrared Spectroscopy Measurements Of Glucose And Xylose In Biomass Hydrolyzate, Czarena L. Crofcheck, Michael D. Montross
Biosystems and Agricultural Engineering Faculty Publications
Measurement of sugars using traditional spectroscopic (UV/Vis) assays or high performance liquid chromatography (HPLC) can be time consuming and expensive. Alternative methods for measuring sugars after enzymatic hydrolysis of biomass would be convenient for screening potential biomass feedstocks and pretreatment methods. Fourier transform infrared spectroscopy (FTIR) has been utilized for measuring composition of various aqueous solutions and is evaluated here as an alternative to UV/Vis and HPLC assays. Solutions of glucose and xylose with concentrations between 0 and 1.5% w/v (total sugar content between 0 and 3.0% w/v) were used to build calibration curves for all three methods. A validation …