Digital Commons Network^™

Mechanistic Examination Of Protist-Mediated Plant Growth Through The Comparative Development Of Medicago Truncatula, Shane Connolly

University Scholar Projects

Protists are known to increase plant growth through two main mechanisms: the microbial loop and the alteration of the root microbiome. The microbial loop is a nutrient recycling method in which protists provide inorganic nitrogen ions to the plant. Alteration of root microbiome leads to the removal of plant pathogens and shifting communities towards plant growth-promoting bacteria (PGPB). This study aimed to elicit which mechanism could produce the largest boost in shoot weight for Medicago truncatula. A series of microcosm experiments were explored in which M. truncatula was grown with variable microbiome structures to allow for mechanism differentiation. The …

Cenabis Bene: A Culinary Odyssey Through Apicius, Kathryn Atkinson

University Scholar Projects

Apicius is the sole surviving cookbook from classical antiquity; as such it is invaluable for what it tells us about ancient feasting customs. Yet the gluttony typically associated with classical antiquity has no place in Apicius beyond the art that is inherently associated with food; we are not so much given a seat at the cena (dinner) as we are led into the kitchen, handed an apron, and instructed to cook. This critical analysis explores each recipe not only on the surface—i.e., examining the ingredients and recreating selected recipes—but also on a deeper level, lifting food above its concrete reality …

Expanding Teacher Diversity And Learning Achievements: Understanding And Supporting The Teaching Career Decision Making Of Minoritized Students, Jannatul Anika

University Scholar Projects

The purpose of this study was to explore how college students of color who are considering (or have considered) the teaching profession describe the internal and external factors that are influencing their career decision. In Connecticut during the 2021-22 academic year, 89.9 percent of public school teachers identified as White, while Connecticut’s population of students of color is more than 45 percent. This project translated the observations and experiences around the lack of diversity in the teaching workforce and aimed to understand the underlying reasons why there is a shortage of teachers of color with the goal of recommending solutions. …

A Zooarchaeological Metadata Analysis Of Animal Domestication In The Neolithic Northern Levant, Ashlyn R. Cartier

University Scholar Projects

This project compiles and examines 17 published faunal assemblages spanning the Epipaleolithic to the Pottery Neolithic in the Northern Levant to uncover early trends in animal management practices. Through a close examination of indices tracking the proportions of low and high-ranked prey, wild versus domestic game, and taxonomic diversity, it is possible to examine the trends in and deduce the approximate timing of the shift toward early animal management practices. To examine the conditions from which this phenomenon emerged, analyses of hunting intensity and taxonomic tradeoffs were implemented. Hunting intensity provides an analysis of low and high-ranked prey to examine …

Shakespearean Constellations, Sarah Bradshaw

University Scholar Projects

Sarah Bradshaw’s thesis argues that Shakespeare's legacy is a fundamentally collaborative product. Rather than viewing Shakespeare’s legacy as the product of a single individual, what "Shakespeare" has come to mean over the past 400 years is altered by those who read, depict, and adapt these texts. Bradshaw presents Shakespeare, Romantic critics, and film adaptors as artists collaborating with their pasts and presents to adapt texts into new environments. By adopting Walter Benjamin’s metaphor of the constellation, Bradshaw theorizes Shakespeare’s legacy as a larger image in which each source, reading, and adaptation operates as a discrete object of study that together …

Mapping Galactic Acceleration With Pulsar Timing, Abigail Moran

University Scholar Projects

We have conducted a cross match of objects in Gaia Early Data Release 3 and millisecond pulsars (MSPs) in the International Pulsar Timing Array’s Data Release 2 (IPTA DR2) to identify binary systems. Gaia has parallax measurements for these optical companions, which we combine with pulsar timing based parallax measurements to calculate new combined MSP distances. Through this crossmatch with IPTA DR2 we improved five distance measurements and found the first parallax measurement for one MSP.

Using this Gaia crossmatch method now with a well-timed subset of the Australia Telescope National Facility’s database, we found three new pulsar distances. We …

Dynamic Transcriptomic Responses Of Circulating Immune Cells In Response To Subsequent Days Of Exercise Heat Stress, Soohyun Oh

University Scholar Projects

Acute exercise and chronic training have diverse effects on immune function that are still not well understood. There are remaining questions about how an acute bout of exercise, competition, or repeated training over a period affects an individual’s immune defense system. In a related study, there is evidence that acute exercise may serve as an adjuvant to enhance response to immune function as well as contradicting evidence, including our preliminary data (unpublished). Understanding the fundamental function and gene expression of circulating immune cells in response to acute exercise and in various contexts such as heat stress is critical in understanding …

A Structured Life Review Intervention To Improve Life Satisfaction In Home Health Service Patients: A Feasibility Study, Leah Graf

University Scholar Projects

Two million older adults in the U.S. suffer with depression. Adults who live with the difficulties of depression concurrently experience dissatisfaction with life. Life review is a systematic recollection of past events. Dr. Robert Butler postulated that, as the elderly and those with terminal illness approach the end of their lives, there is a resurgence of life experiences in the conscience (1963). A successful life review conducted by an active listener assists the individual to process unresolved conflicts and attain ego-integrity in the final stage of life. Home health services have become an increasingly accepted option for managing chronic illnesses …

The Effect Of Fibrodysplasia Ossificans Progressiva On The Tongue, Amy Backal, Amanda Harrop, David J. Goldhamer Dr.

University Scholar Projects

FOP is a rare genetic disorder in which skeletal muscle and associated connective tissue progressively turn to bone through a process called heterotopic ossification (HO). The extra skeletal bone growth is cumulative, eventually trapping patients in a second skeleton that eventually leads to death by asphyxiation. The FOP mutation is autosomal dominant that can be inherited or acquired sporadically. Unfortunately, FOP is currently incurable with no therapeutic options to inhibit bone growth or reduce existing bone nodules. My project intends to further our understanding of the cellular mechanisms of the disease within the tongue muscle. A population of cells known …

Textiles And The Portrayal Of Power: Figuring European-Ottoman Relations, 16th-17th Centuries, Kathryn A. Krocheski

University Scholar Projects

The exploration of the complex relationship between Tudor England and the Ottoman Empire in the sixteenth-century through Turkish textiles and motifs present in Tudor portraiture.

Novel Epigenetic Therapeutics For The Treatment Of Opioid Use Disorder, Suzannah De Almeida

University Scholar Projects

Epigenetic-based therapeutics are promising treatment options for Substance Use Disorder (SUD) due to their ability to influence gene expression and reverse behavioral responses associated with addiction. For example, previous studies have shown that epigenetic reader proteins, called Bromodomains and Extra Terminal Domain (BET), are increased in the nucleus accumbens following cocaine self-administration and that pharmacological inhibition of BET proteins reduced cocaine-seeking behaviors in rodents. Although BET inhibitors reduced cocaine- seeking behaviors in animal models, a role for BET proteins in animal models of Opioid Use Disorder (OUD) remains unknown. To identify a role for BET proteins in OUD, we measured …

Identification Of Translesion Synthesis Inhibitors That Target Rev7/Rev3 Protein-Protein Interactions, Seema Patel

University Scholar Projects

Translesion synthesis (TLS) is a cellular mechanism utilized by cancer cells to tolerate DNA damage caused by chemotherapeutics, like cisplatin, by replicating past unrepaired lesions. This increases the rate of mutations, which leads to the emergence of drug-resistant cancer cells. Preliminary studies have shown that disrupting the protein-protein interactions (PPI) in the TLS heteroprotein complex increases cells’ sensitivity to first-line genotoxic chemotherapy, illustrating how inhibiting TLS assembly and function can significantly increase cancer cell death. These results underscore the therapeutic potential of targeting TLS PPI. My thesis focuses on identifying inhibitors capable of disrupting the Rev7/Rev3 TLS PPI. This study …

Optimization Of Orbital Trajectories Using Neuroevolution Of Augmenting Topologies, Nathan Wetherell

University Scholar Projects

This project aims to determine the feasibility of using NeuroEvolution of Augmenting Topologies (NEAT), an advanced neural network evolution scheme, to optimize orbital transfer trajectories. More specifically, this project compares a genetically evolved neural network to a standard Hohmann transfer between Earth and Mars. To test these two methods, an N-body simulation environment was created to accurately determine the result of gravitational interactions on a theoretical spacecraft when combined with planned engine burns. Once created, this simulation environment was used to train the neural networks created using the NEAT Python module. A genetic algorithm was used to modify the topology …

When Problems Become Solutions: Harnessing The Osteogenic Capacity Of Disease-Causing Stem Cells To Repair Bone Fractures, Mehreen Pasha

University Scholar Projects

While we often perceive disease as negative, there is potential to engineer seemingly negative biological phenomena into therapeutics to treat a variety of human illnesses. Fibrodysplasia ossificans progressiva (FOP) is a genetic disorder involving uncontrolled, widespread, extraskeletal bone growth, or heterotopic ossification (HO). In FOP patients, stem cells called fibro/adipogenic progenitors (FAPs) follow an abnormal, osteogenic pathway. In the present study, we investigate whether we can adapt these Acvr1 mutant FAPs, which are exceptional at producing bone, to repair bone fractures in otherwise normal patients. The primary aims of this study are (1) to devise and optimize a novel method …

Computational Investigations Into Binding Dynamics Of Tau Protein Antibodies: Using Machine Learning And Biophysical Models To Build A Better Reality, Katherine Lee

University Scholar Projects

Misregulation of post-translational modifications of microtubule-associated protein tau is implicated in several neurodegenerative diseases including Alzheimer’s disease. Hyperphosphorylation of tau promotes aggregation of tau monomers into filaments which are common in tau-associated pathologies. Therefore, tau is a promising target for therapeutics and diagnostics. Recently, high-affinity, high-specificity single-chain variable fragment (scFv) antibodies against pThr-231 tau were generated and the most promising variant (scFv 3.24) displayed 20-fold increased binding affinity to pThr-231 tau compared to the wild-type. The scFv 3.24 variant contained five point mutations, and intriguingly none were in the tau binding site. The increased affinity was hypothesized to occur due …

Foe To Friend: Parallel Domestication Of Ophiocordyceps From Fungal Parasite To Beneficial Symbiont In Cicadas, Jason Vailionis, Eric Rl Gordon, Chris Simon

University Scholar Projects

Nutritional symbioses are integral to the survival and diversity of many insects. The majority of herbivorous insects in the order Hemiptera possess stable, inherited symbionts that produce essential amino acids and vitamins. However, instability has been observed in cicadas, with one bacterial symbiont, Hodgkinia cicadicola, being repeatedly replaced by a new fungal symbiont, Ophiocordyceps. The fungal symbionts are thought to be derived from parasitic Ophiocordyceps species, but little is known about these parasitic ancestors or how the transition from parasite to mutualist occurs. We used a combination of targeted amplified genes and metagenomic sequencing to investigate the evolution …

Identifying The Cell Composition And Clonal Diversity Of Supratentorial Ependymoma Using Single Cell Rna-Sequencing, James He

University Scholar Projects

Ependymoma is a primary solid tumor of the central nervous system. Supratentorial ependymoma (ST-EPN), a subtype of ependymomas, is driven by an oncogenic fusion between the ZFTA and RELA genes in 70% of cases. We introduced this fusion into neural progenitor cells of mice embryos via in utero electroporation of a non-viral binary piggyBac transposon system containing ZFTA-RELA. From preliminary data in the LoTurco lab, inducing the expression of ZFTA-RELA into different neural progenitor cells produces tumors of varying lethality and cellular composition. To define the cellular composition and subclonal diversity of ST-EPN tumors, we used single cell RNA-sequencing to …

Linking Human Activities To Water Quality In Coastal New England: Past And Present, Amelia Hurst

University Scholar Projects

This project examines the timing and effect of direct and indirect anthropogenic and natural influences on the marine environment in embayments in southern New England over the past decades to century timescale. We investigated the effects of human land use from colonial through post-industrial times, determined baseline conditions and natural climatic variability, and analyzed the response of marine ecosystems to specific local management actions aimed to improve water quality. A coastal sediment core was taken in Mumford Cove, CT and was analyzed downcore for eutrophication markers (C: N, %C, %N, d¹⁵N, d¹³C) and metals (Hg, Pb, …

Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films, Jayla Millender

University Scholar Projects

One of the most common causes of bone graft rejection is lack of a vascular network connecting the graft to the existing native tissue – allowing for nutrient flow. Under current grafting techniques, the existing blood vessel network in the patient slowly invades the implant in order to supply the injured site with its necessary nutrients. The purpose of this research is to determine if a synthetic bone graft with a stable microvascular network can be developed in vitro. I hypothesize that the use of indirect angiogenic factors such as sonic hedgehog homolog and hypoxia-inducible factor-1 in combination with the …

The Effects Of Mapk Signaling On The Development Of Cerebellar Granule Cells, Kerry Morgan

University Scholar Projects

The granule cells are the most abundant neuronal type in the human brain. Rapid proliferation of granule cell progenitors results in dramatic expansion and folding of the cerebellar cortex during postnatal development. Mis-regulation of this proliferation process causes medulloblastoma, the most prevalent childhood brain tumor. In the developing cerebellum, granule cells are derived from Atoh1-expressing cells, which arise from the upper rhombic lip (the interface between the roof plate and neuroepithelium). In addition to granule cells, the Atoh1 lineage also gives rise to different types of neurons including cerebellar nuclei neurons. In the current study, I have investigated the …

Knowing China, Losing China: Discourse And Power In U.S.-China Relations, Shankara Narayanan

University Scholar Projects

The U.S. government’s 2017 National Security Strategy claimed, “China and Russia challenge American power, influence, and interests, attempting to erode American security and prosperity.”[1] Three years later, the COVID-19 pandemic has accelerated the U.S. foreign policy community’s discursive shift towards Realist competition with China, with officials from the past three presidential administrations coming to view China as a threat to democratic governance and America’s security posture in Asia. The discourse underpinning the U.S.-China relationship, however, remains understudied. During key moments in the relationship, U.S. policymakers’ Realist intellectual frameworks failed to account for Chinese nationalism, suggesting a problem embedded within …

The Generalized Riemann Hypothesis And Applications To Primality Testing, Peter Hall

University Scholar Projects

The Riemann Hypothesis, posed in 1859 by Bernhard Riemann, is about zeros
of the Riemann zeta-function in the complex plane. The zeta-function can be repre-
sented as a sum over positive integers n of terms 1/ns when s is a complex number
with real part greater than 1. It may also be represented in this region as a prod-
uct over the primes called an Euler product. These definitions of the zeta-function
allow us to find other representations that are valid in more of the complex plane,
including a product representation over its zeros. The Riemann Hypothesis says that
all …

The Sdss-Rm Project: Uv/Optical Accretion Disk Measurements For Supermassive Black Holes With Hubble Space Telescope, Megan Sturm

University Scholar Projects

We report accretion-disk structure measurements for eight rapidly accreting supermassive black holes selected from the Sloan Digital Sky Survey Reverberation Mapping project sample. Reverberation mapping uses light echoes to measure disk size from the time lag between variability in the inner/hotter and outer/cooler disk emission. We use Hubble Space Telescope ultraviolet observations coordinated with optical monitoring from the Liverpool Telescope and Las Cumbres Observatory. We find ten significant UV/optical lags for five out of the eight total targets. Through these time lags, we study the accretion disk as a function of disk size, temperature profile and radiative efficiency. We find …

The Enterprise Of Health: An Evaluation Of The Accessibility Of Durable Medical Equipment In Low-Income Households, Simran Sehgal

University Scholar Projects

People with disabilities constitute a marginalized group in the United States and their experiences within the health care system are often unaccounted for in policy recommendations and reforms. This research aimed to provide general insights on the barriers that prevent access to durable medical equipment (DME) for people with disabilities. I developed a qualitative ethnographic study to capture how people with disabilities experience these barriers first-hand. I collected five in-depth semi-structured interviews with people with disabilities in need of DME. The findings are grouped into two large themes, the economic and the social. The goal of the research is to …

Untold Stories Of The African Diaspora: The Lived Experiences Of Black Caribbean Immigrants In The Greater Hartford Area, Shanelle A. Jones

University Scholar Projects

The African Diaspora represents vastly complex migratory patterns. This project studies the journeys of English-speaking Afro-Caribbeans who immigrated to the US for economic reasons between the 1980s-present day. While some researchers emphasize the success of West Indian immigrants, others highlight the issue of downward assimilation many face upon arrival in the US. This paper explores the prospect of economic incorporation into American society for West Indian immigrants. I conducted and analyzed data from an online survey and 10 oral histories of West Indian economic migrants residing in the Greater Hartford Area to gain a broader perspective on the economic attainment …

Study Of A Putative Niche Adapting Operon In Microbes Inhabiting The Gut Of Blood Digesting Animals, Marlene Abouaassi

University Scholar Projects

The sialic acid utilization (SAU) operon is a horizontally acquired gene set that allows bacteria to utilize sialic acid as an alternate source of carbon and nitrogen in the guts of blood eating animals. Sialic acid often occurs as a terminal sugar in complex glycoproteins. It functions in cell signaling and adhesion. Sialic acid is an important component of the cellular envelope of animals. Some microorganisms have evolved to decorate their own surface with sialic acid to evade the host’s immune response (molecular mimicry). The SAU operon encodes enzymes that hydrolyze sialic acid from glycoproteins, transport sialic acid into the …

Cultural Food Habits As A Social Factor Of Health Among Immigrants In New Haven, Connecticut: A Focused Ethnographic Study, Luke Anderson

University Scholar Projects

Diet-related health disparities are well documented in immigrant populations. This study aims to help better inform nutrition interventions. It did so by working with migrant members of the New Haven community to explore their perceptions of the nutrition of the food they eat and relate it to how this food is grounded in their cultural identity and social belonging.

Design Of Versatile Feedback Control System Components For Selective Laser Sintering, Thomas Chessman

University Scholar Projects

Selective laser sintering (SLS) is an additive manufacturing technique that involves using a laser to fuse powdered material together, layer by layer, in order to create a 3-D product. Despite its numerous benefits over traditional methods of manufacturing, including higher efficiency, versatility, and the ability to process many materials, selective laser sintering suffers from its propensity to generate structural errors during operation.

Feedback control has been shown to improve fabrication quality in other laser-based additive manufacturing techniques when implemented properly. Widespread exploration of applying feedback control in SLS might lead to significant performance improvements in this form of manufacturing.

This …

Detection Of Tinnitus In Cba/Caj Mice Using The Active Avoidance Shuttle Box Test, Grace Nichols

University Scholar Projects

Tinnitus is a neurological condition that involves the perception of a sound that is not actually there. Individuals affected with tinnitus describe the condition as a ringing, buzzing, or whooshing sound in their ears. One-third of the population is estimated to have tinnitus, and for many individuals, the condition negatively impacts quality of life. Difficulty falling asleep, trouble with hearing and concentrating, and in some cases, depression, have all been observed to occur with tinnitus.

Tinnitus has been extensively studied in animals, and behavioral tests are the primary method of evaluating the presence of tinnitus in animals. This study evaluates …

Genetic Analysis Of Flower Color Differences Between A Hummingbird-Pollinated And A Self-Pollinated Monkeyflower (Mimulus) Species, Caitlin Foster

University Scholar Projects

Flower color plays an important role in pollinator discrimination and speciation. Understanding the genetic contributions to flower color differences between two closely related species, Mimulus cardinalis and Mimulus parishii, can improve understanding of how they developed different pollination syndromes and diverged from a recent common ancestor. M. cardinalis is hummingbird-pollinated and has large, bright red flowers while M. parishii is self-pollinated and has small, pale pink flowers. An F2 hybrid population between these two species was created to establish a platform for analysis of the genetic architecture controlling the differences in anthocyanin pigmentation. Statistical analysis of anthocyanin concentration distribution …