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Thermal Inactivation Of Salmonella, Escherichia Coli, And Enterococcus Faecium Nrrl B-2354 In Pasta Matrices, Kristi Shannon Gowans

Theses and Dissertations

Limited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during mixing, extrusion, and drying. These data are essential to pasta producers seeking to be compliant with federal regulations since Salmonella spp. and Escherichia coli demonstrate survival in wheat flour and dried pasta products. This study investigated the heat resistance of Salmonella, pathogenic E. coli, and E. faecium NRRL B-2354 in raw semolina flour and partially dried pasta intermediates via thermal death time (TDT) studies. This study also assessed the appropriateness of E. faecium NRRL B-2354 as …

Isothermal Inactivation Studies Of Listeria Monocytogenes, Salmonella, And Enterococcus Faecium Nrrl B-2354 In Almond, Peanut, And Sunflower Butters, Ruo Fen Liao

Theses and Dissertations

Vegetative, non-sporeforming foodborne pathogens show notable survival and uncanny thermotolerance in low water activity (aw) foods. Controlled studies on Listeria monocytogenes, Salmonella spp., and Enterococcus faecium NRRL B-2354 (a Salmonella surrogate) in a variety of food matrices support thermal process validation studies required to achieve global food safety objectives. In this study, we determined and compared thermal inactivation rates using independent six-strain cocktails of pathogens in three plant-based butters. Direct determinations of decimal reduction times (D-values) for L. monocytogenes, Salmonella, and E. faecium, in corresponding butters were inoculated using peanut oil, almond oil, or sunflower oil. Thermal Death Time (TDT) …

Improving Nutrition And Food Safety Knowledge Of Small-Scale Fish Processors In Delta State, Nigeria, Grace Adeola Adegoye

Theses and Dissertations

Introduction: Fish is an affordable animal source food that provides nutrition and serves as a source of income for many people especially women in Nigeria. Smoking and sun drying are the processing methods in practice that expose the fish products to possible contaminants which may consequentially negate their nutritive value.

Aim: To improve the knowledge of fish processors on nutrition and safe fish handling.

Methodology: A 3-day participatory training was organized to train 122 fish processors, 95 women, and 25 men. The training was conducted in the three senatorial districts in Delta State, Nigeria. Knowledge was assessed using the pre …

An Examination Of Capacity Building For Sanitary And Phytosanitary Measures For Women In Sub-Saharan Africa: Empowerment Theory At The Individual, Organizational, And Community Levels, Lisa De Leon

Theses and Dissertations

Knowledge and application gaps exist for women farmers in Sub-Saharan Africa who are key agricultural players for economic growth and food security. This study examined capacity development for Sanitary and Phytosanitary (SPS) measures and empowerment of women farmers considering Rappaport (1984) and Zimmerman’s (1995, 2000) lenses of empowerment theory. The central research question was, how does capacity development for sanitary and phytosanitary measures empower women at the individual, organizational, and community levels in Sub-Saharan Africa? The study employed an embedded mixed methods design collecting data via an electronic survey from 23 Sub-Saharan women farmers; 22 from Ghana and one from …

Isothermal Inactivation Of Salmonella, Listeria Monocytogenes, And Enterococcus Faecium Nrrl-B 2354 In Peanut Butter, Powder Infant Formula, And Wheat Flour, Adam Robert Quinn

Theses and Dissertations

Pathogens in low-moisture foods are an emerging food safety concern due to increased survival and thermotolerance in matrices with low water activity. However, limited data is publicly available for the thermotolerance of Listeria monocytogenes, Salmonella spp., and Enterococcus faecium NRRL B-2354 (a Salmonella surrogate). The aims of this study were to identify differences in thermal inactivation rates between these organisms in three different low-moisture foods. Three model low-moisture foods (peanut butter, powder infant formula, and wheat flour) were inoculated with either E. faecium, a Salmonella spp. cocktail, or a L. monocytogenes cocktail using a dry inoculation method for a total …

Hospital And Meat Associated Staphylococcus Aureus And Their Biofilm Characteristics, Trevor Michael Wienclaw

Theses and Dissertations

Biofilm phenotypes were studied in 32 Staphylococcus aureus strains isolated from store-bought meats and 22 from diseased patients in hospitals. Of the meat-associated strains, 21 were methicillin-resistant Staphylococcus aureus (MRSA) and 11 were methicillin-susceptible Staphylococcus aureus (MSSA). The hospital-associated strains included 15 MRSAs and 7 MSSAs. We studied the robustness and composition of the biofilms produced by these strains. We found that on average hospital-associated strains form more robust biofilms than meat associated strains. The model often used to describe S. aureus biofilm composition includes two biofilm types defined by the presence or absence of polysaccharide intercellular adhesin (PIA), PIA-dependent …

Evaluation Of Urea Hydrolysis As A Biomarker For Detecting Pathogenic Vibrio Parahaemolyticus In Clinical Isolates And Raw Oysters, Maryam Kate Mohammadi-Aragh

Theses and Dissertations

Vibrio parahaemolyticus (Vp) infection is commonly caused by the consumption of raw or undercooked shellfish. Raw oysters are associated with most Vp outbreaks. Pathogenic Vp express thermostable-direct hemolysin (tdh) and to some extent thermostable-related hemolysin (trh). Additionally, some pathogenic Vp express urease (uh). The objectives of this work were to discern any relationships between urease (uh) expression, tdh/trh expression, and hemolytic activity in pathogenic and non-pathogenic clinical strains, and to compare urease, motility, tdh/trh expression, and hemolytic activity in raw oyster isolates. This information would determine if urease could be used as a biomarker to detect pathogenic Vp. About 80% …

Evaluating The Impact Of Cetylpyridinium Chloride And Peroxyacetic Acid When Applied To Broiler Frames On Salmonella Spp. And The Quality And Sensory Attributes Of Mechanically Deboned Chicken Meat, Saxon Morgaine Perez

Theses and Dissertations

Broiler frames were treated with antimicrobial solutions of 0.5% cetylpyridinium chloride with CitrilowTM (CPC) or 0.1% peroxyacetic acid (PAA) for contact times of 30 to 120 sec and evaluated for their effectiveness at reducing the incidence of Salmonella spp. on broiler frames and decreasing Salmonellae counts on mechanically separated chicken meat (MSC). MSC samples were evaluated for pH, color, cook loss and protein bind. Results from the preliminary study indicated that 0.5% CPC at 60, 90, and 120 sec achieved higher reduction of Salmonellae on whole broiler frames than CPC at 30 sec or any PAA treatments. In contrast, PAA …

Spectral Band Selection For Ensemble Classification Of Hyperspectral Images With Applications To Agriculture And Food Safety, Sathishkumar Samiappan

Theses and Dissertations

In this dissertation, an ensemble non-uniform spectral feature selection and a kernel density decision fusion framework are proposed for the classification of hyperspectral data using a support vector machine classifier. Hyperspectral data has more number of bands and they are always highly correlated. To utilize the complete potential, a feature selection step is necessary. In an ensemble situation, there are mainly two challenges: (1) Creating diverse set of classifiers in order to achieve a higher classification accuracy when compared to a single classifier. This can either be achieved by having different classifiers or by having different subsets of features for …

A Comparison Of Participant Gains In Attitude And Behavior After Experiencing A Food Safety Curriculum In Traditional And Computer Delivered Environments, Jennifer Knowles Schilling

Theses and Dissertations

Child care providers in Mississippi are required by the Mississippi Health Department to obtain food manager’s training and certification. The TummySafe© program satisfies this requirement and is offered in a self-paced computer delivered version and a traditional classroom version. This research explores participant changes in attitude and self-reported behaviors in the two methods of curriculum delivery as well as the correlation of knowledge change with attitude and self-reported behavior change. A quasi-experimental, pre-test/post-test design was used. Attitude change was not significantly different in the two methods. Traditional participants reported a higher change in self-reported behaviors than computer delivered participants. Both …