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Global Analysis Of Cell Infection And Virus Production On Hiv-1 Dynamics, Jin Xu

Digitized Theses

More than one sub-type of HIVs have been identified. This raises an issue of co-infections by multiple strains of HIVs. In this thesis, we propose two mathematical models, one ignoring intracellular delay and the other incorporating the delay, to describe the interactions of the populations of CD4+cells and two HIV stains. By nature, the two strains compete for CD4+ cells to invade for their own replications. By analyzing the two models, we find that the models demonstrate threshold dynamics: if the overall basic reproduction number Ro < 1, then the infection free equilibrium is globally asymptotically stable; when R0 > 1, then the competition exclusion principle generically holds in the sense that, except for …

Generation Of Long-Lived Dendritic Cells For A Dendritic Cell-Based Therapeutic Hiv Vaccine, Ryan Nicholas Buensuceso

Digitized Theses

Despite advances in therapy, acquired immune deficiency syndrome (AIDS) continues to be a global problem. New therapeutic avenues are being explored, including dendritic cell (DC)-based immunotherapy. While DCs can efficiently promote an immune response, their limited lifespan within the lymph node represents an obstacle to efficient immunotherapy.

;We examined different gene transfer methods, observing lentiviral transduction to be the most effective. Transduction using generated lentiviral transfer vectors encoding M11L and EGFP were used to determine effects on cellular viability. We did not observe significant differences in viability following apoptosis induction in transduced L cells. In primary DC cultures, transduction with …